trpc3  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Alomone Labs trpc3
    Trpc3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trpc3/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    trpc3 - by Bioz Stars, 2022-12
    95/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Alomone Labs anti trpc3 antibody
    Expression levels of store-operated Ca 2+ entry channels in REM sleep deprivation with and without lithium treatment. Compared with the REM sleep-deprived hearts ( n = 5), the lithium-treated REM sleep-deprived hearts ( n = 5) had lower expression levels of calcium release-activated calcium channel protein 1 (Orai1), transient receptor potential canonical (TRPC) 1 channel, and <t>TRPC3</t> channel. * p
    Anti Trpc3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpc3 antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpc3 antibody - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs antibody against trpc3
    Canagliflozin reduced vasoconstriction by inhibiting high‐salt‐induced <t>TRPC3‐reverse</t> mode NCX1 complexes. A , Effects of SN6 (NCX1 pan inhibitor, 10 μmol/L) and pyr3 on U46619‐ and PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type mice. n=8. B , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3‐oe and si‐TRPC3 plasmids and treated with control, HS, SN6 (10 μmol/L SN6), and HS+SN6 (20 mmol/L Nacl+10 μmol/L SN6). The quantitative results are shown on the right. n=12. C , The relative protein expression levels of NCX1 in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe and si‐TRPC3 plasmids and treated with control (Con), HS, and HS+canagliflozin (HS+Cana). D , Effects of SN6 and KB‐R7943 (reverse mode NCX1 inhibitor, 10 μmol/L) on U46619‐ or PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type mice fed with high‐salt diet. n=8. E and F , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3oe and si‐TRPC3 plasmids and treated with control, HS, KB‐R7943 (10 μmol/L KB‐R7943), and HS+KB‐R7943 (20 mmol/L NaCL+10 μmol/L KB‐R7943). The quantitative results are shown on the right. n=12. G and H , Effects of SN6 and KB‐R7943 on U46619‐ and PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type and TRPC3 −/− mice fed with high‐salt diet. n=8. I , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs treated with HS, HS+canagliflozin, pyr3+canagliflozin (20 mmol/L NaCL+10 μmol/L pyr3+10 μmol/L canagliflozin), KB‐R7943+canagliflozin (20 mmol/L NaCL+10 μmol/L KB‐R7943+10 μmol/L canagliflozin), and pyr3+KB‐R7943 (20 mmol/L NaCL+10 μmol/L pyr3+10 μmol/L KB‐R7943). The quantitative results are shown on the right. n=12. J , The relative mRNA expression levels of TRPC3 and NCX1 in VSMCs treated with control, HS, HS+canagliflozin, HS+pyr3, HS+SN6, and HS+KB‐R7943. n=8. K , Input and Immunoprecipitation of TRPC3 and NCX1 in VSMCs and immunoblotted by TRPC3 and NCX1 <t>antibody.</t> n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P
    Antibody Against Trpc3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against trpc3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against trpc3 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    Expression levels of store-operated Ca 2+ entry channels in REM sleep deprivation with and without lithium treatment. Compared with the REM sleep-deprived hearts ( n = 5), the lithium-treated REM sleep-deprived hearts ( n = 5) had lower expression levels of calcium release-activated calcium channel protein 1 (Orai1), transient receptor potential canonical (TRPC) 1 channel, and TRPC3 channel. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Lithium Treatment Improves Cardiac Dysfunction in Rats Deprived of Rapid Eye Movement Sleep

    doi: 10.3390/ijms231911226

    Figure Lengend Snippet: Expression levels of store-operated Ca 2+ entry channels in REM sleep deprivation with and without lithium treatment. Compared with the REM sleep-deprived hearts ( n = 5), the lithium-treated REM sleep-deprived hearts ( n = 5) had lower expression levels of calcium release-activated calcium channel protein 1 (Orai1), transient receptor potential canonical (TRPC) 1 channel, and TRPC3 channel. * p

    Article Snippet: Blots were probed with the following antibodies against regulator proteins involved in cardiac fibrogenesis: TGF-β (1:2000, polyclonal, #3711; Cell Signaling Technology, Beverly, MA, USA), TGF-β RI (1:1000, polyclonal, #sc-398; Santa Cruz Biotechnology, Santa Cruz, CA, USA), total Smad 2/3 (1:2000, monoclonal, #8685; Cell Signaling Technology), phosphorylated Smad 2/3 (1:2000, monoclonal, #8828; Cell Signaling Technology), α-SMA (1:5000, monoclonal, #ab7817; Abcam, Cambridge, UK), AGTR1 (1:2000, polyclonal, #AAR-011; Alomone Labs, Jerusalem, Israel), total NF-κB p65 (1:2000, monoclonal, #8242; Cell Signaling Technology), phosphorylated NF-κB p65 (1:2000, monoclonal, #3033; Cell Signaling Technology), Orai1 (1:2000, polyclonal, #4281; ProSci Incorporated, Poway, CA, USA), STIM1 (1:2000, monoclonal, #610954; BD Transduction Laboratories, San Diego, CA, USA), TRPC 1 channel (1:2000, polyclonal, #ACC-010; Alomone Labs), TRPC3 (1:5000, polyclonal, #ACC-016; Alomone Labs), and TRPC6 (1:2000, polyclonal, #ACC-017; Alomone Labs).

    Techniques: Expressing

    Schematic illustration of the proposed mechanisms for cardioprotection of lithium in REM sleep deprivation. Lithium may improve ventricular dysfunction and cardiac fibrosis in REM sleep-deprived hearts via the downregulation of the transforming growth factor beta signaling pathway, angiotensin II cascade, and store-operated Ca 2+ entry. Abbreviations: α-SMA = α-smooth muscle actin; AGTR1 = angiotensin II receptor type 1; NF-κB = nuclear factor kappa B; Orai1 = calcium release-activated calcium channel protein 1; REM = rapid eye movement; TGF-β = transforming growth factor beta; TGF-β R1 = transforming growth factor beta receptor 1; TRPC1 = transient receptor potential canonical 1 channel; TRPC3 = transient receptor potential canonical 1 channel.

    Journal: International Journal of Molecular Sciences

    Article Title: Lithium Treatment Improves Cardiac Dysfunction in Rats Deprived of Rapid Eye Movement Sleep

    doi: 10.3390/ijms231911226

    Figure Lengend Snippet: Schematic illustration of the proposed mechanisms for cardioprotection of lithium in REM sleep deprivation. Lithium may improve ventricular dysfunction and cardiac fibrosis in REM sleep-deprived hearts via the downregulation of the transforming growth factor beta signaling pathway, angiotensin II cascade, and store-operated Ca 2+ entry. Abbreviations: α-SMA = α-smooth muscle actin; AGTR1 = angiotensin II receptor type 1; NF-κB = nuclear factor kappa B; Orai1 = calcium release-activated calcium channel protein 1; REM = rapid eye movement; TGF-β = transforming growth factor beta; TGF-β R1 = transforming growth factor beta receptor 1; TRPC1 = transient receptor potential canonical 1 channel; TRPC3 = transient receptor potential canonical 1 channel.

    Article Snippet: Blots were probed with the following antibodies against regulator proteins involved in cardiac fibrogenesis: TGF-β (1:2000, polyclonal, #3711; Cell Signaling Technology, Beverly, MA, USA), TGF-β RI (1:1000, polyclonal, #sc-398; Santa Cruz Biotechnology, Santa Cruz, CA, USA), total Smad 2/3 (1:2000, monoclonal, #8685; Cell Signaling Technology), phosphorylated Smad 2/3 (1:2000, monoclonal, #8828; Cell Signaling Technology), α-SMA (1:5000, monoclonal, #ab7817; Abcam, Cambridge, UK), AGTR1 (1:2000, polyclonal, #AAR-011; Alomone Labs, Jerusalem, Israel), total NF-κB p65 (1:2000, monoclonal, #8242; Cell Signaling Technology), phosphorylated NF-κB p65 (1:2000, monoclonal, #3033; Cell Signaling Technology), Orai1 (1:2000, polyclonal, #4281; ProSci Incorporated, Poway, CA, USA), STIM1 (1:2000, monoclonal, #610954; BD Transduction Laboratories, San Diego, CA, USA), TRPC 1 channel (1:2000, polyclonal, #ACC-010; Alomone Labs), TRPC3 (1:5000, polyclonal, #ACC-016; Alomone Labs), and TRPC6 (1:2000, polyclonal, #ACC-017; Alomone Labs).

    Techniques:

    Canagliflozin reduced vasoconstriction by inhibiting high‐salt‐induced TRPC3‐reverse mode NCX1 complexes. A , Effects of SN6 (NCX1 pan inhibitor, 10 μmol/L) and pyr3 on U46619‐ and PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type mice. n=8. B , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3‐oe and si‐TRPC3 plasmids and treated with control, HS, SN6 (10 μmol/L SN6), and HS+SN6 (20 mmol/L Nacl+10 μmol/L SN6). The quantitative results are shown on the right. n=12. C , The relative protein expression levels of NCX1 in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe and si‐TRPC3 plasmids and treated with control (Con), HS, and HS+canagliflozin (HS+Cana). D , Effects of SN6 and KB‐R7943 (reverse mode NCX1 inhibitor, 10 μmol/L) on U46619‐ or PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type mice fed with high‐salt diet. n=8. E and F , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3oe and si‐TRPC3 plasmids and treated with control, HS, KB‐R7943 (10 μmol/L KB‐R7943), and HS+KB‐R7943 (20 mmol/L NaCL+10 μmol/L KB‐R7943). The quantitative results are shown on the right. n=12. G and H , Effects of SN6 and KB‐R7943 on U46619‐ and PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type and TRPC3 −/− mice fed with high‐salt diet. n=8. I , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs treated with HS, HS+canagliflozin, pyr3+canagliflozin (20 mmol/L NaCL+10 μmol/L pyr3+10 μmol/L canagliflozin), KB‐R7943+canagliflozin (20 mmol/L NaCL+10 μmol/L KB‐R7943+10 μmol/L canagliflozin), and pyr3+KB‐R7943 (20 mmol/L NaCL+10 μmol/L pyr3+10 μmol/L KB‐R7943). The quantitative results are shown on the right. n=12. J , The relative mRNA expression levels of TRPC3 and NCX1 in VSMCs treated with control, HS, HS+canagliflozin, HS+pyr3, HS+SN6, and HS+KB‐R7943. n=8. K , Input and Immunoprecipitation of TRPC3 and NCX1 in VSMCs and immunoblotted by TRPC3 and NCX1 antibody. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Sodium‐Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt‐Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling

    doi: 10.1161/JAHA.121.025328

    Figure Lengend Snippet: Canagliflozin reduced vasoconstriction by inhibiting high‐salt‐induced TRPC3‐reverse mode NCX1 complexes. A , Effects of SN6 (NCX1 pan inhibitor, 10 μmol/L) and pyr3 on U46619‐ and PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type mice. n=8. B , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3‐oe and si‐TRPC3 plasmids and treated with control, HS, SN6 (10 μmol/L SN6), and HS+SN6 (20 mmol/L Nacl+10 μmol/L SN6). The quantitative results are shown on the right. n=12. C , The relative protein expression levels of NCX1 in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe and si‐TRPC3 plasmids and treated with control (Con), HS, and HS+canagliflozin (HS+Cana). D , Effects of SN6 and KB‐R7943 (reverse mode NCX1 inhibitor, 10 μmol/L) on U46619‐ or PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type mice fed with high‐salt diet. n=8. E and F , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3oe and si‐TRPC3 plasmids and treated with control, HS, KB‐R7943 (10 μmol/L KB‐R7943), and HS+KB‐R7943 (20 mmol/L NaCL+10 μmol/L KB‐R7943). The quantitative results are shown on the right. n=12. G and H , Effects of SN6 and KB‐R7943 on U46619‐ and PE‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type and TRPC3 −/− mice fed with high‐salt diet. n=8. I , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs treated with HS, HS+canagliflozin, pyr3+canagliflozin (20 mmol/L NaCL+10 μmol/L pyr3+10 μmol/L canagliflozin), KB‐R7943+canagliflozin (20 mmol/L NaCL+10 μmol/L KB‐R7943+10 μmol/L canagliflozin), and pyr3+KB‐R7943 (20 mmol/L NaCL+10 μmol/L pyr3+10 μmol/L KB‐R7943). The quantitative results are shown on the right. n=12. J , The relative mRNA expression levels of TRPC3 and NCX1 in VSMCs treated with control, HS, HS+canagliflozin, HS+pyr3, HS+SN6, and HS+KB‐R7943. n=8. K , Input and Immunoprecipitation of TRPC3 and NCX1 in VSMCs and immunoblotted by TRPC3 and NCX1 antibody. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P

    Article Snippet: For western blot assays, the primary antibody against TRPC3 was purchased from Alomone (Alomone Labs), and the primary antibody against MYPT1 and phosphorylated MYPT1 was purchased from Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Isolation, Mouse Assay, Expressing, Immunoprecipitation

    Schematic diagram of this work. TRPC3 and NCX1 maintain dynamic calcium balance and participate in vascular activities under physiological conditions. However, upregulation of TRPC3 by high salt leads to intracellular Na + accumulation and then activated reverse mode of NCX1, which promotes sustained vasoconstriction caused by increased cytosolic calcium concentration. This might be an important reason for salt‐sensitive hypertension. The antihypertensive effects of canagliflozin are mediated to decreasing cytosolic calcium concentration and vasoconstriction through inhibition of TRPC3. NCX1 indicates sodium‐calcium exchanger 1; and TRPC3, transient receptor potential channels 3.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Sodium‐Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt‐Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling

    doi: 10.1161/JAHA.121.025328

    Figure Lengend Snippet: Schematic diagram of this work. TRPC3 and NCX1 maintain dynamic calcium balance and participate in vascular activities under physiological conditions. However, upregulation of TRPC3 by high salt leads to intracellular Na + accumulation and then activated reverse mode of NCX1, which promotes sustained vasoconstriction caused by increased cytosolic calcium concentration. This might be an important reason for salt‐sensitive hypertension. The antihypertensive effects of canagliflozin are mediated to decreasing cytosolic calcium concentration and vasoconstriction through inhibition of TRPC3. NCX1 indicates sodium‐calcium exchanger 1; and TRPC3, transient receptor potential channels 3.

    Article Snippet: For western blot assays, the primary antibody against TRPC3 was purchased from Alomone (Alomone Labs), and the primary antibody against MYPT1 and phosphorylated MYPT1 was purchased from Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Concentration Assay, Inhibition

    Canagliflozin regulated calcium influx in vascular smooth muscle cells mediated by TRPC3. A , Representative images of the immunofluorescence staining of TRPC3, NCX1, and DAPI using mesenteric arteries isolated from Dahl salt‐sensitive and Dahl salt‐insensitive rats. Bar represents 25 μm. Quantification of the intensities in each graph was presented in the right 2 panels. n=8. B , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe, and si‐TRPC3 plasmids and treated with control, HS, and HS+canagliflozin. The quantitative results are shown on the right. n=12. C , Cytosolic calcium imaging of VSMCs transferred with TRPC3‐nc, TRPC3‐oe, and si‐TRPC3 plasmids and treated with control, HS, and HS+canagliflozin, The relative fluorescence intensity of VSMCs were quantified. n=12. D , Effect of acute Pyr3 (TRPC3 inhibitor, 10 μmol/L) preincubation on PE‐ and U46619‐induced vasoconstriction of the mesenteric artery rings from Dahl salt‐sensitive fed with ND and HS. n=8. E , Representative western blots of TRPC3, CamkII, and phosphorylation of CamkII in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe, and si‐TRPC3 plasmids and treated with control (Con), HS, and HS+canagliflozin (HS+Cana). β‐actin served as a loading control. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Sodium‐Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt‐Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling

    doi: 10.1161/JAHA.121.025328

    Figure Lengend Snippet: Canagliflozin regulated calcium influx in vascular smooth muscle cells mediated by TRPC3. A , Representative images of the immunofluorescence staining of TRPC3, NCX1, and DAPI using mesenteric arteries isolated from Dahl salt‐sensitive and Dahl salt‐insensitive rats. Bar represents 25 μm. Quantification of the intensities in each graph was presented in the right 2 panels. n=8. B , [Ca 2+ ] cyt in ER Ca 2+ release and Ca 2+ uptake in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe, and si‐TRPC3 plasmids and treated with control, HS, and HS+canagliflozin. The quantitative results are shown on the right. n=12. C , Cytosolic calcium imaging of VSMCs transferred with TRPC3‐nc, TRPC3‐oe, and si‐TRPC3 plasmids and treated with control, HS, and HS+canagliflozin, The relative fluorescence intensity of VSMCs were quantified. n=12. D , Effect of acute Pyr3 (TRPC3 inhibitor, 10 μmol/L) preincubation on PE‐ and U46619‐induced vasoconstriction of the mesenteric artery rings from Dahl salt‐sensitive fed with ND and HS. n=8. E , Representative western blots of TRPC3, CamkII, and phosphorylation of CamkII in mouse VSMCs transferred with TRPC3‐nc, TRPC3‐oe, and si‐TRPC3 plasmids and treated with control (Con), HS, and HS+canagliflozin (HS+Cana). β‐actin served as a loading control. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P

    Article Snippet: For western blot assays, the primary antibody against TRPC3 was purchased from Alomone (Alomone Labs), and the primary antibody against MYPT1 and phosphorylated MYPT1 was purchased from Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Immunofluorescence, Staining, Isolation, Imaging, Fluorescence, Western Blot

    Reduction of vasoconstriction by canagliflozin was dependent on TRPC3. A , The time course of SBP in wild‐type and TRPC3 −/− mice fed with ND, HS, and HS+canagliflozin for 24 weeks. n=8. B , The 24th week of SBP in wild‐type and TRPC3 −/− mice fed with ND, HS, and HS+canagliflozin for 24 weeks. N=8. C , Representative images of hematoxylin–eosin staining cross‐sections of mesenteric arteries isolated from wild‐type and TRPC3 −/− mice. Statistical analysis of mesenteric artery wall thickness, lumen diameter, and the ratio of artery wall to the lumen. Scale bar denotes 25 μm. N=8. D and E , PE‐ and U46619‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type and TRPC3 −/− mice. n=8. F , Representative images of the immunofluorescence staining of TRPC3, NCX1, and DAPI using mesenteric arteries from wild‐type and TRPC3 −/− mice. Bar represents 25 μm. Quantification of the intensities in each graph was presented in the right 2 panels. n=8. G and H , Representative western blots of TRPC3, NCX1, CamkII, MYPT‐1, p‐MYPT‐1, MLC2, and p‐MLC2 in carotid artery from wild‐type and TRPC3 −/− mice fed with ND, HS, and HS+canagliflozin (HS+Cana). β‐actin served as a loading control. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Sodium‐Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt‐Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling

    doi: 10.1161/JAHA.121.025328

    Figure Lengend Snippet: Reduction of vasoconstriction by canagliflozin was dependent on TRPC3. A , The time course of SBP in wild‐type and TRPC3 −/− mice fed with ND, HS, and HS+canagliflozin for 24 weeks. n=8. B , The 24th week of SBP in wild‐type and TRPC3 −/− mice fed with ND, HS, and HS+canagliflozin for 24 weeks. N=8. C , Representative images of hematoxylin–eosin staining cross‐sections of mesenteric arteries isolated from wild‐type and TRPC3 −/− mice. Statistical analysis of mesenteric artery wall thickness, lumen diameter, and the ratio of artery wall to the lumen. Scale bar denotes 25 μm. N=8. D and E , PE‐ and U46619‐induced vasoconstriction of the mesenteric artery rings isolated from wild‐type and TRPC3 −/− mice. n=8. F , Representative images of the immunofluorescence staining of TRPC3, NCX1, and DAPI using mesenteric arteries from wild‐type and TRPC3 −/− mice. Bar represents 25 μm. Quantification of the intensities in each graph was presented in the right 2 panels. n=8. G and H , Representative western blots of TRPC3, NCX1, CamkII, MYPT‐1, p‐MYPT‐1, MLC2, and p‐MLC2 in carotid artery from wild‐type and TRPC3 −/− mice fed with ND, HS, and HS+canagliflozin (HS+Cana). β‐actin served as a loading control. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferroni's posttest for multiple comparisons. * P

    Article Snippet: For western blot assays, the primary antibody against TRPC3 was purchased from Alomone (Alomone Labs), and the primary antibody against MYPT1 and phosphorylated MYPT1 was purchased from Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Mouse Assay, Staining, Isolation, Immunofluorescence, Western Blot

    Canagliflozin inhibited the vascular contraction and calcium channel‐related protein pathways. A , Venn diagram in groups between upregulated proteins of ND vs HS and downregulated proteins of HS vs HS+canagliflozin. B , The KEGG pathways enriched by differentially expressed proteins in groups between upregulated proteins of ND vs HS and downregulated proteins of HS vs HS+canagliflozin. C and D , The vascular‐associated biological process terms (GO) enriched by differentially expressed proteins proteins in groups between upregulated proteins of ND vs HS and down‐regulated proteins of HS vs HS+canagliflozin. E and F , Representative western blots of TRPC3, NCX1, CamkII, MYPT‐1, MLC2, and phosphorylation of MYPT‐1 and MLC2 (p‐MYPT‐1 and p‐MLC2) in carotid artery from Dahl salt‐sensitive and Dahl salt‐insensitive rats. β‐actin served as a loading control. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferron’'s posttest for multiple comparisons. A 2‐tailed Fishe’'s exact test was used in the GO KEGG enrichment analyses. * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Sodium‐Glucose Cotransporter 2 Inhibitor Canagliflozin Antagonizes Salt‐Sensitive Hypertension Through Modifying Transient Receptor Potential Channels 3 Mediated Vascular Calcium Handling

    doi: 10.1161/JAHA.121.025328

    Figure Lengend Snippet: Canagliflozin inhibited the vascular contraction and calcium channel‐related protein pathways. A , Venn diagram in groups between upregulated proteins of ND vs HS and downregulated proteins of HS vs HS+canagliflozin. B , The KEGG pathways enriched by differentially expressed proteins in groups between upregulated proteins of ND vs HS and downregulated proteins of HS vs HS+canagliflozin. C and D , The vascular‐associated biological process terms (GO) enriched by differentially expressed proteins proteins in groups between upregulated proteins of ND vs HS and down‐regulated proteins of HS vs HS+canagliflozin. E and F , Representative western blots of TRPC3, NCX1, CamkII, MYPT‐1, MLC2, and phosphorylation of MYPT‐1 and MLC2 (p‐MYPT‐1 and p‐MLC2) in carotid artery from Dahl salt‐sensitive and Dahl salt‐insensitive rats. β‐actin served as a loading control. n=8. Results are expressed as mean±SEM. Statistical significance was determined by unpaired Student t test for comparisons between 2 groups and 1‐way ANOVA followed by Bonferron’'s posttest for multiple comparisons. A 2‐tailed Fishe’'s exact test was used in the GO KEGG enrichment analyses. * P

    Article Snippet: For western blot assays, the primary antibody against TRPC3 was purchased from Alomone (Alomone Labs), and the primary antibody against MYPT1 and phosphorylated MYPT1 was purchased from Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Western Blot