glua2 (Alomone Labs)


Structured Review

Glua2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glua2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly"
Article Title: α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly
Journal: Cell reports
doi: 10.1016/j.celrep.2021.109396

Figure Legend Snippet: α2δ-1 reduces surface and synaptic expression of GluA2 and heteromeric GluA1/GluA2 receptors (A) Original blots and quantification show the protein levels of GluA1 and GluA2 in HEK293 cells cotransfected with GluA1/GluA2 and control vector (pcDNA3, P3), α2δ-1, α2δ-2, or α2δ-3 (n = 4 per group). ***p
Techniques Used: Expressing, Plasmid Preparation

Figure Legend Snippet: α2δ-1 disrupts the heteromeric assembly of GluA1/GluA2 and increases GluA2 retention in the endoplasmic reticulum (A and B) Original blots (A) and quantification (B) show that α2δ-1 coexpression diminishes heteromeric GluA1/GluA2 receptors in the ER of HEK293 cells. n = 5 per group. *p
Techniques Used:

Figure Legend Snippet: α2δ-1 physically interacts with GluA1 and GluA2 in vitro and in vivo (A) CoIP shows the interaction between α2δ-1 and GluA1 and GluA2 in HEK293 cells. Cells cotransfected with GFP-tagged α2δ-1 and GluA1, GluA2, GluA1/GluA2, or FLAG-stargazin (STG). (B) CoIP shows the interaction of α2δ-1 with homomeric GluA1 or GluA2 in HEK293 cells. P3, control vector. (C) CoIP shows the interaction of α2δ-1 with heteromeric GluA1/GluA2 in HEK293 cells. (D) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 in the dorsal spinal cord of rats subjected to a sham procedure (S) or spinal nerve ligation (L). (E) CoIP shows the interaction of α2δ-1 with GluA1 and GluA2 subunits in the normal spinal cord tissue of two human donors (S1 and S2). (F) α2δ-1 interacts with GluA1 and GluA2 subunits via its C terminus. HEK293 cells were cotransfected with GluA1/GluA2 and various PC-tagged α2δ-1 constructs. δ-1ΔCT, δ-1 without the C terminus; CT, the C terminus of δ-1; VWA, von Willebrand factor type A domain. (G) CoIP shows that α2δ-1CT-Tat peptide disrupts the α2δ-1 interaction with GluA1 and GluA2 in HEK293 cells. Cell were cotransfected with GluA1/GluA2 and α2δ-1 or FLAG-α2δ-1 and were treated with 1 μM α2δ-1CT-Tat peptide (Pept) or 1 μM Tat-fused Cont peptide for 30 min. ***p
Techniques Used: In Vitro, In Vivo, Co-Immunoprecipitation Assay, Plasmid Preparation, Ligation, Construct

Figure Legend Snippet: Gabapentin and the α2δ-1CT-Tat peptide normalize synaptic expression of GluA2-containing AMPARs in the spinal cord diminished in neuropathic pain (A and B) Original blots and quantification show the effect of gabapentin and α2δ-1CT-Tat peptide on the protein levels of GluA1 and GluA2 in the spinal cord synaptosome (A) and the ER (B) of sham and SNL rats (n = 6 rats per group). Spinal cord slices were treated with vehicle (Cont), 100 μM GBP, 1 μM control peptide (P(−)), or 1 μM α2δ-1CT-Tat peptide (P(+)). **p
Techniques Used: Expressing

Figure Legend Snippet: Gabapentin and the α2δ-1CT-Tat C terminus peptide restore heteromeric GluA1/GluA2 receptors diminished by α2δ-1 coexpression (A and B) Original GCaMP images and signals show intracellular Ca 2+ changes in response to 5 mM glutamate (Glut) in HEK293 cells transfected with GluA1/GluA2 (A) or GluA1/GluA2/α2δ-1 (B). (C) Mean data show effects of treatment with vehicle (n = 54 cells), gabapentin (100 μM, n = 28 cells), α2δ-1CT-Tat peptide (1 μM, n = 26 cells), or control peptide (1 μM, n = 21 cells) on the ratio (ΔF/F0) of GCaMP signals elicited by glutamate. ***p
Techniques Used: Transfection