anti cav1 2  (Alomone Labs)


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    Structured Review

    Alomone Labs anti cav1 2
    The splicing factor RNA-binding motif 20 (RBM20) regulates the inclusion of alternatively spliced exon 9*. ( A ) The scheme represents the <t>α1C</t> subunit of l -type voltage calcium channels. CaV1.2 (α1C) is composed of four homologous regions consisting of six transmembrane domains (S1–S6) that form the pore of the channel. (+) indicates voltage sensors on S4. There are two types of alternatively spliced exons on CaV1.2: [ 1 ] Mutually exclusive exons—1/1a, 8/8a, 21/22, and 31/32 (the corresponding region is shown in dark)—and [ 2 ] optional exons—9* and 33 (shown as dotted lines). ( B ) The graphs show RBM20 expression measured by quatitative RT-PCR. The left graph is the expression of RBM20 measured in the control, Green Fluorescent protein (GFP)-overexpressing, and RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 10 total RNA extracts from 10 cardiomyocyte isolations (gray circle). The right graph shows the expression in the control, luciferase-1-targeting siRNA (siLuc) tranfected cardiomyocytes, and siRNA-targeting RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 5 total RNA extracts from 5 cardiomyocyte isolations (gray circle). * means p
    Anti Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav1 2/product/Alomone Labs
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    anti cav1 2 - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes"

    Article Title: RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20225591

    The splicing factor RNA-binding motif 20 (RBM20) regulates the inclusion of alternatively spliced exon 9*. ( A ) The scheme represents the α1C subunit of l -type voltage calcium channels. CaV1.2 (α1C) is composed of four homologous regions consisting of six transmembrane domains (S1–S6) that form the pore of the channel. (+) indicates voltage sensors on S4. There are two types of alternatively spliced exons on CaV1.2: [ 1 ] Mutually exclusive exons—1/1a, 8/8a, 21/22, and 31/32 (the corresponding region is shown in dark)—and [ 2 ] optional exons—9* and 33 (shown as dotted lines). ( B ) The graphs show RBM20 expression measured by quatitative RT-PCR. The left graph is the expression of RBM20 measured in the control, Green Fluorescent protein (GFP)-overexpressing, and RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 10 total RNA extracts from 10 cardiomyocyte isolations (gray circle). The right graph shows the expression in the control, luciferase-1-targeting siRNA (siLuc) tranfected cardiomyocytes, and siRNA-targeting RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 5 total RNA extracts from 5 cardiomyocyte isolations (gray circle). * means p
    Figure Legend Snippet: The splicing factor RNA-binding motif 20 (RBM20) regulates the inclusion of alternatively spliced exon 9*. ( A ) The scheme represents the α1C subunit of l -type voltage calcium channels. CaV1.2 (α1C) is composed of four homologous regions consisting of six transmembrane domains (S1–S6) that form the pore of the channel. (+) indicates voltage sensors on S4. There are two types of alternatively spliced exons on CaV1.2: [ 1 ] Mutually exclusive exons—1/1a, 8/8a, 21/22, and 31/32 (the corresponding region is shown in dark)—and [ 2 ] optional exons—9* and 33 (shown as dotted lines). ( B ) The graphs show RBM20 expression measured by quatitative RT-PCR. The left graph is the expression of RBM20 measured in the control, Green Fluorescent protein (GFP)-overexpressing, and RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 10 total RNA extracts from 10 cardiomyocyte isolations (gray circle). The right graph shows the expression in the control, luciferase-1-targeting siRNA (siLuc) tranfected cardiomyocytes, and siRNA-targeting RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 5 total RNA extracts from 5 cardiomyocyte isolations (gray circle). * means p

    Techniques Used: RNA Binding Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Luciferase

    2) Product Images from "RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes"

    Article Title: RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20225591

    The splicing factor RNA-binding motif 20 (RBM20) regulates the inclusion of alternatively spliced exon 9*. ( A ) The scheme represents the α1C subunit of l -type voltage calcium channels. CaV1.2 (α1C) is composed of four homologous regions consisting of six transmembrane domains (S1–S6) that form the pore of the channel. (+) indicates voltage sensors on S4. There are two types of alternatively spliced exons on CaV1.2: [ 1 ] Mutually exclusive exons—1/1a, 8/8a, 21/22, and 31/32 (the corresponding region is shown in dark)—and [ 2 ] optional exons—9* and 33 (shown as dotted lines). ( B ) The graphs show RBM20 expression measured by quatitative RT-PCR. The left graph is the expression of RBM20 measured in the control, Green Fluorescent protein (GFP)-overexpressing, and RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 10 total RNA extracts from 10 cardiomyocyte isolations (gray circle). The right graph shows the expression in the control, luciferase-1-targeting siRNA (siLuc) tranfected cardiomyocytes, and siRNA-targeting RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 5 total RNA extracts from 5 cardiomyocyte isolations (gray circle). * means p
    Figure Legend Snippet: The splicing factor RNA-binding motif 20 (RBM20) regulates the inclusion of alternatively spliced exon 9*. ( A ) The scheme represents the α1C subunit of l -type voltage calcium channels. CaV1.2 (α1C) is composed of four homologous regions consisting of six transmembrane domains (S1–S6) that form the pore of the channel. (+) indicates voltage sensors on S4. There are two types of alternatively spliced exons on CaV1.2: [ 1 ] Mutually exclusive exons—1/1a, 8/8a, 21/22, and 31/32 (the corresponding region is shown in dark)—and [ 2 ] optional exons—9* and 33 (shown as dotted lines). ( B ) The graphs show RBM20 expression measured by quatitative RT-PCR. The left graph is the expression of RBM20 measured in the control, Green Fluorescent protein (GFP)-overexpressing, and RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 10 total RNA extracts from 10 cardiomyocyte isolations (gray circle). The right graph shows the expression in the control, luciferase-1-targeting siRNA (siLuc) tranfected cardiomyocytes, and siRNA-targeting RBM20-overexpressing cardiomyocytes. Data are the mean and SEM (black circle) of n = 5 total RNA extracts from 5 cardiomyocyte isolations (gray circle). * means p

    Techniques Used: RNA Binding Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Luciferase

    3) Product Images from "The Oxidative Stress-Induced Increase in the Membrane Expression of the Water-Permeable Channel Aquaporin-4 in Astrocytes Is Regulated by Caveolin-1 Phosphorylation"

    Article Title: The Oxidative Stress-Induced Increase in the Membrane Expression of the Water-Permeable Channel Aquaporin-4 in Astrocytes Is Regulated by Caveolin-1 Phosphorylation

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2017.00412

    Cav1 Y14 phosphorylation enhances AQP4 expression at the cell surface in MDA-435 cells. Immunoblot demonstrating the expression of the VSV-AQP4 transgene in MDA-435 cell lines carrying an empty vector, or expressing wild-type (WT), Y14F (dominant-negative) and Y14D (phosphomimetic) variants of Cav1, with β-actin shown as a loading control (A) . Cell-surface, intracellular, and total AQP4 levels in MDA-435 cells expressing WT, Y14F and Y14D Cav1 (B) . Quantification of the above ( C ; n = 3 independent experiments; * p
    Figure Legend Snippet: Cav1 Y14 phosphorylation enhances AQP4 expression at the cell surface in MDA-435 cells. Immunoblot demonstrating the expression of the VSV-AQP4 transgene in MDA-435 cell lines carrying an empty vector, or expressing wild-type (WT), Y14F (dominant-negative) and Y14D (phosphomimetic) variants of Cav1, with β-actin shown as a loading control (A) . Cell-surface, intracellular, and total AQP4 levels in MDA-435 cells expressing WT, Y14F and Y14D Cav1 (B) . Quantification of the above ( C ; n = 3 independent experiments; * p

    Techniques Used: Expressing, Multiple Displacement Amplification, Plasmid Preparation, Dominant Negative Mutation

    The Src kinase inhibitor 4-Amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2) suppresses the H 2 O 2 -induced increase in AQP4 expression at the cell surface. Immunoblots comparing the level of caveolin-1 (Cav1) Y14 phosphorylation in astrocytes exposed to various concentrations of H 2 O 2 for 1 h (A) , or 200 μM H 2 O 2 for increasing lengths of time (B) . Immunoblot depicting the effects of 1 h pre-incubation with increasing concentrations of PP2 prior to an hour-long treatment with 200 μM H 2 O 2 on Cav1 Y14 phosphorylation (C) . Cell-surface, intracellular, and total AQP4 levels in control and PP2-pre-treated astrocytes in the presence and absence of a 1 h-long H 2 O 2 treatment (D) , and a histogram depicting the relative, input-normalized cell-surface AQP4 amounts under these various conditions ( E ; n = 3 independent experiments; * p
    Figure Legend Snippet: The Src kinase inhibitor 4-Amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2) suppresses the H 2 O 2 -induced increase in AQP4 expression at the cell surface. Immunoblots comparing the level of caveolin-1 (Cav1) Y14 phosphorylation in astrocytes exposed to various concentrations of H 2 O 2 for 1 h (A) , or 200 μM H 2 O 2 for increasing lengths of time (B) . Immunoblot depicting the effects of 1 h pre-incubation with increasing concentrations of PP2 prior to an hour-long treatment with 200 μM H 2 O 2 on Cav1 Y14 phosphorylation (C) . Cell-surface, intracellular, and total AQP4 levels in control and PP2-pre-treated astrocytes in the presence and absence of a 1 h-long H 2 O 2 treatment (D) , and a histogram depicting the relative, input-normalized cell-surface AQP4 amounts under these various conditions ( E ; n = 3 independent experiments; * p

    Techniques Used: Expressing, Western Blot, Incubation

    Loss of Cav1 inhibits the H 2 O 2 -induced increase in AQP4 cell-surface expression in astrocytes. Cav1 expression in control- (siCtl) and Cav1-siRNA (siCav1)-transfected astrocytes (A) . Quantification of the above ( B ; n = 3 independent experiments; * p
    Figure Legend Snippet: Loss of Cav1 inhibits the H 2 O 2 -induced increase in AQP4 cell-surface expression in astrocytes. Cav1 expression in control- (siCtl) and Cav1-siRNA (siCav1)-transfected astrocytes (A) . Quantification of the above ( B ; n = 3 independent experiments; * p

    Techniques Used: Expressing, Transfection

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  • 94
    Alomone Labs anti cav1 2
    Anti Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav1 2/product/Alomone Labs
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti cav1 2 - by Bioz Stars, 2022-12
    94/100 stars
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