guinea pig polyclonal antikir4 1 antibody  (Alomone Labs)


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    Alomone Labs guinea pig polyclonal antikir4 1 antibody
    Guinea Pig Polyclonal Antikir4 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    guinea pig polyclonal antikir4 1 antibody - by Bioz Stars, 2023-01
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    guinea pig polyclonal antikir4 1 antibody  (Alomone Labs)


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    Alomone Labs guinea pig polyclonal antikir4 1 antibody
    Guinea Pig Polyclonal Antikir4 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig polyclonal antikir4 1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    guinea pig polyclonal antikir4 1 antibody - by Bioz Stars, 2023-01
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    anti kir4 1 antibody produced in guinea pig  (Alomone Labs)


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    Alomone Labs anti kir4 1 antibody produced in guinea pig
    List of primary antibodies used
    Anti Kir4 1 Antibody Produced In Guinea Pig, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir4 1 antibody produced in guinea pig/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir4 1 antibody produced in guinea pig - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain"

    Article Title: Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain

    Journal: Journal of Neuroscience Research

    doi: 10.1002/jnr.24967

    List of primary antibodies used
    Figure Legend Snippet: List of primary antibodies used

    Techniques Used: Concentration Assay, Produced, Recombinant, Purification, Derivative Assay, Transduction

    Antibodies used and amino acid conservation between species
    Figure Legend Snippet: Antibodies used and amino acid conservation between species

    Techniques Used:

    Heterogeneity of cortical astrocytes. Hierarchical clustering algorithm identified astrocytic clusters based on the KS distance displayed in the dendrogram (left panel on each row) for EAAT2 (A), GAT3 (B), and Kir4.1 (C). Distance measurements reveal significant separation of astrocytes into distinct clusters. Three different images from two different animals were analyzed for each marker. The spatial organization of astrocytes within clusters is represented a, b, and c. Each astrocyte was identified using GS expression and it was assigned to a region from the pial surface (Region 1, R1) to inner cortical layers (R2, R3, and R4). Scale bars: 50 μm. Each astrocyte was identified with a number, R1 and R3 numbers are indicated in white and R2 and R4 numbers indicated in yellow. Analysis of GS can be found in Figure . Every cluster was identified by a number and particular color and the percentage of cells in each cluster in regions 1–4 is shown in a′–c′
    Figure Legend Snippet: Heterogeneity of cortical astrocytes. Hierarchical clustering algorithm identified astrocytic clusters based on the KS distance displayed in the dendrogram (left panel on each row) for EAAT2 (A), GAT3 (B), and Kir4.1 (C). Distance measurements reveal significant separation of astrocytes into distinct clusters. Three different images from two different animals were analyzed for each marker. The spatial organization of astrocytes within clusters is represented a, b, and c. Each astrocyte was identified using GS expression and it was assigned to a region from the pial surface (Region 1, R1) to inner cortical layers (R2, R3, and R4). Scale bars: 50 μm. Each astrocyte was identified with a number, R1 and R3 numbers are indicated in white and R2 and R4 numbers indicated in yellow. Analysis of GS can be found in Figure . Every cluster was identified by a number and particular color and the percentage of cells in each cluster in regions 1–4 is shown in a′–c′

    Techniques Used: Marker, Expressing

    Heterogeneity of hippocampal astrocytes. Hierarchical clustering algorithm identified different astrocytic clusters based on the KS distance displayed in the dendrogram (left panel on each row) for GFAP (A), AQP4 (B), and Kir4.1 (C). The spatial organization of astrocytes within clusters is represented at the right side of each image (a–c). Scale bars: 50 μm. Each astrocyte was detected using GS expression and it was assigned in S. radiatum, S. pyramidale, and S. oriens. Each astrocyte was identified with a number, S. radiatum and S. oriens in white and S. pyramidale in yellow. Analysis of GS, EAAT2, and GAT3 can be found in Figure . Every astrocyte within a cluster was identified and assigned a number with a particular color. The percentage of cells in each cluster in the S. oriens (Ori), S. pyramidale (Pyr), and S. radiatum (Rad) is shown in a′–c′
    Figure Legend Snippet: Heterogeneity of hippocampal astrocytes. Hierarchical clustering algorithm identified different astrocytic clusters based on the KS distance displayed in the dendrogram (left panel on each row) for GFAP (A), AQP4 (B), and Kir4.1 (C). The spatial organization of astrocytes within clusters is represented at the right side of each image (a–c). Scale bars: 50 μm. Each astrocyte was detected using GS expression and it was assigned in S. radiatum, S. pyramidale, and S. oriens. Each astrocyte was identified with a number, S. radiatum and S. oriens in white and S. pyramidale in yellow. Analysis of GS, EAAT2, and GAT3 can be found in Figure . Every astrocyte within a cluster was identified and assigned a number with a particular color. The percentage of cells in each cluster in the S. oriens (Ori), S. pyramidale (Pyr), and S. radiatum (Rad) is shown in a′–c′

    Techniques Used: Expressing

    GFAP labels marmoset cortical astrocytic populations including complex interlaminar astrocytes. (A–E) Sparsely labeled GFAP‐positive astrocytes across cortical layers. The approximate location of cortical layers indicated by Roman numerals I to IV (yellow). Orange arrowheads show interlaminar processes and green arrowheads define protoplasmic astrocytes (A). Detailed images of an interlaminar astrocyte (B), protoplasmic astrocyte (C), astrocytic endfeet (D), and white matter astrocytes (E). DAPI (blue) is used as a nuclear marker and AQP4 (blue) as an endfoot marker. Scale bar: 50 μm (A), 20 μm (B, C, and E), and 5 μm (D). Subtypes of interlaminar astrocytes (ILA). Marmoset pial ILAs (F–J) and subpial ILAs (K–O) labeled by S100β (F, K), GS (G, L), Sox9 (H, M) and specific astrocytic markers such as Kir4.1 (I, N) and AQP4 (J, O). Yellow arrowheads show astrocytic soma and processes in F–O. Orange arrowheads describe a punctate AQP4 staining. Scale bars: 20 μm (F–O)
    Figure Legend Snippet: GFAP labels marmoset cortical astrocytic populations including complex interlaminar astrocytes. (A–E) Sparsely labeled GFAP‐positive astrocytes across cortical layers. The approximate location of cortical layers indicated by Roman numerals I to IV (yellow). Orange arrowheads show interlaminar processes and green arrowheads define protoplasmic astrocytes (A). Detailed images of an interlaminar astrocyte (B), protoplasmic astrocyte (C), astrocytic endfeet (D), and white matter astrocytes (E). DAPI (blue) is used as a nuclear marker and AQP4 (blue) as an endfoot marker. Scale bar: 50 μm (A), 20 μm (B, C, and E), and 5 μm (D). Subtypes of interlaminar astrocytes (ILA). Marmoset pial ILAs (F–J) and subpial ILAs (K–O) labeled by S100β (F, K), GS (G, L), Sox9 (H, M) and specific astrocytic markers such as Kir4.1 (I, N) and AQP4 (J, O). Yellow arrowheads show astrocytic soma and processes in F–O. Orange arrowheads describe a punctate AQP4 staining. Scale bars: 20 μm (F–O)

    Techniques Used: Labeling, Marker, Staining

    Expression of glutamate and GABA transporters, and the potassium channel Kir4.1 in the marmoset cerebral cortex. Double immunostaining of excitatory amino acid transport 2 (EAAT2) and GS (A), GABA transporter 3 (GAT3) and GS (B) and inward rectifier‐type potassium channel Kir4.1 and GS (C) in coronal sections from marmoset temporal cortex. GS was used as a counter‐label due to its more homogenous distribution in cortical layers. a′–c′ show higher magnifications of white rectangle areas of merged images. Right panels show a heat map of each specific marker showing its expression density through cortical layers. Purple‐to‐blue color indicates lower intensity signal, while the red‐to‐yellow color means higher intensity signal. Scale bars: 50 μm in A–C and 20 μm in a′–c′. bv, blood vessel
    Figure Legend Snippet: Expression of glutamate and GABA transporters, and the potassium channel Kir4.1 in the marmoset cerebral cortex. Double immunostaining of excitatory amino acid transport 2 (EAAT2) and GS (A), GABA transporter 3 (GAT3) and GS (B) and inward rectifier‐type potassium channel Kir4.1 and GS (C) in coronal sections from marmoset temporal cortex. GS was used as a counter‐label due to its more homogenous distribution in cortical layers. a′–c′ show higher magnifications of white rectangle areas of merged images. Right panels show a heat map of each specific marker showing its expression density through cortical layers. Purple‐to‐blue color indicates lower intensity signal, while the red‐to‐yellow color means higher intensity signal. Scale bars: 50 μm in A–C and 20 μm in a′–c′. bv, blood vessel

    Techniques Used: Expressing, Double Immunostaining, Marker

    Expression of glutamate and GABA transporters, Kir4.1, and AQP4 in the marmoset hippocampus. Labeling of coronal sections of marmoset hippocampus with astrocytic markers associated with subpopulations of astrocytes. Double immunolabeling of EAAT2 and GS (A), GAT3 and GS (B), Kir4.1 and GS (C) and water channel Aquaporin 4 (AQP4) and GS (D). The third panel of each row shows a merged image and the fourth panel is a heat map showing the density of expression of each specific marker. A purple–blue color indicates lower intensity signal and red–yellow color means higher intensity signal. White arrows highlight areas of particularly high signal intensity. Scale bars: 50 μm
    Figure Legend Snippet: Expression of glutamate and GABA transporters, Kir4.1, and AQP4 in the marmoset hippocampus. Labeling of coronal sections of marmoset hippocampus with astrocytic markers associated with subpopulations of astrocytes. Double immunolabeling of EAAT2 and GS (A), GAT3 and GS (B), Kir4.1 and GS (C) and water channel Aquaporin 4 (AQP4) and GS (D). The third panel of each row shows a merged image and the fourth panel is a heat map showing the density of expression of each specific marker. A purple–blue color indicates lower intensity signal and red–yellow color means higher intensity signal. White arrows highlight areas of particularly high signal intensity. Scale bars: 50 μm

    Techniques Used: Expressing, Labeling, Immunolabeling, Marker

    Interactions of marmoset astrocytes with capillaries, neurons, and microglia. (A) Schema showing the gliovascular unit and blood vessel‐associated microglia. (B) GFAP and S100β labeling in astrocytic processes and endfeet surrounding a capillary in the marmoset cortex. Yellow circle shows high S100β expression and red circles highlight low S100β expression. Scale bar: 20 µm. (C) Cross‐sectional view of a capillary in the marmoset hippocampus displaying expression of AQP4 and GFAP at the astrocyte endfeet. Yellow arrowheads indicate GFAP expression at endfeet and white arrowheads indicate areas of co‐localization between GFAP and AQP4. Laminin (Lam) and DAPI labels show the basement membrane and nuclei of endothelial cells and pericytes (green arrowheads), respectively. Scale bars: 5 µm. (D) Cross‐sectional view of a capillary showing expression of Kir4.1 and GFAP at astrocyte endfeet and DAPI in endothelial cell and pericyte nuclei. Yellow arrowheads indicate GFAP expression, white arrowheads show areas of co‐localization between GFAP and Kir4.1, and green arrowheads highlight nuclei. Scale bars: 5 µm. (E–I) Co‐labeling of GFAP with neuronal markers including MAP2 (E, temporal cortex; G, hippocampus), Kv2.1 (F, temporal cortex; H hippocampus), and Calbindin (I, cerebellum). Higher magnification images are shown to the right of each panel (panels e′–i′). White arrowheads highlight the tight spatial neuronal‐astrocyte interaction. (J–L) Co‐labeling of GFAP with the microglial marker Iba1 in temporal cortex (J and K) and hippocampus (L). Images to the right of each panel (panels j′–l′) show higher magnifications. (J) Vessel‐associated microglia. Higher magnification images show microglia around the blood vessel (orange arrowheads) and microglia attached at the capillary (white arrowheads) (panels j′, j″). White arrowheads highlight the close spatial microglial–astrocyte interaction. Scale bars in panels E–L are 50 µm and e′–l′ are 20 µm. bv, blood vessel
    Figure Legend Snippet: Interactions of marmoset astrocytes with capillaries, neurons, and microglia. (A) Schema showing the gliovascular unit and blood vessel‐associated microglia. (B) GFAP and S100β labeling in astrocytic processes and endfeet surrounding a capillary in the marmoset cortex. Yellow circle shows high S100β expression and red circles highlight low S100β expression. Scale bar: 20 µm. (C) Cross‐sectional view of a capillary in the marmoset hippocampus displaying expression of AQP4 and GFAP at the astrocyte endfeet. Yellow arrowheads indicate GFAP expression at endfeet and white arrowheads indicate areas of co‐localization between GFAP and AQP4. Laminin (Lam) and DAPI labels show the basement membrane and nuclei of endothelial cells and pericytes (green arrowheads), respectively. Scale bars: 5 µm. (D) Cross‐sectional view of a capillary showing expression of Kir4.1 and GFAP at astrocyte endfeet and DAPI in endothelial cell and pericyte nuclei. Yellow arrowheads indicate GFAP expression, white arrowheads show areas of co‐localization between GFAP and Kir4.1, and green arrowheads highlight nuclei. Scale bars: 5 µm. (E–I) Co‐labeling of GFAP with neuronal markers including MAP2 (E, temporal cortex; G, hippocampus), Kv2.1 (F, temporal cortex; H hippocampus), and Calbindin (I, cerebellum). Higher magnification images are shown to the right of each panel (panels e′–i′). White arrowheads highlight the tight spatial neuronal‐astrocyte interaction. (J–L) Co‐labeling of GFAP with the microglial marker Iba1 in temporal cortex (J and K) and hippocampus (L). Images to the right of each panel (panels j′–l′) show higher magnifications. (J) Vessel‐associated microglia. Higher magnification images show microglia around the blood vessel (orange arrowheads) and microglia attached at the capillary (white arrowheads) (panels j′, j″). White arrowheads highlight the close spatial microglial–astrocyte interaction. Scale bars in panels E–L are 50 µm and e′–l′ are 20 µm. bv, blood vessel

    Techniques Used: Labeling, Expressing, Marker

    guinea pig polyclonal  (Alomone Labs)


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    Alomone Labs guinea pig polyclonal
    Guinea Pig Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    anti kir4 1 guinea pig polyclonal antibody  (Alomone Labs)


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    Alomone Labs anti kir4 1 guinea pig polyclonal antibody
    A. Protein levels of <t>Kir4.1</t> measured by Western blot in astrocytes grown in DMEM containing normal glucose and transfected with miRNA-205 mimic (mimic 205) were significantly down-regulated compared to mock-transfected astrocytes (61.4 ± 6.62%; n=5; *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.
    Anti Kir4 1 Guinea Pig Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir4 1 guinea pig polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir4 1 guinea pig polyclonal antibody - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Kir4.1 potassium channel regulation via miR-205 in astrocytes exposed to hyperglycemic conditions."

    Article Title: Kir4.1 potassium channel regulation via miR-205 in astrocytes exposed to hyperglycemic conditions.

    Journal: Neuroreport

    doi: 10.1097/WNR.0000000000001427

    A. Protein levels of Kir4.1 measured by Western blot in astrocytes grown in DMEM containing normal glucose and transfected with miRNA-205 mimic (mimic 205) were significantly down-regulated compared to mock-transfected astrocytes (61.4 ± 6.62%; n=5; *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.
    Figure Legend Snippet: A. Protein levels of Kir4.1 measured by Western blot in astrocytes grown in DMEM containing normal glucose and transfected with miRNA-205 mimic (mimic 205) were significantly down-regulated compared to mock-transfected astrocytes (61.4 ± 6.62%; n=5; *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.

    Techniques Used: Western Blot, Transfection, Incubation, Staining, Molecular Weight

    A. Proteins levels of Kir.4.1 measured by Western blot in astrocytes grown in DMEM containing high glucose and transfected with miRNA-205 inhibitor (Inhibitor 205) were significantly up-regulated compare to mock-transfected astrocytes (147.4 ± 8.6%; n=5, *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.
    Figure Legend Snippet: A. Proteins levels of Kir.4.1 measured by Western blot in astrocytes grown in DMEM containing high glucose and transfected with miRNA-205 inhibitor (Inhibitor 205) were significantly up-regulated compare to mock-transfected astrocytes (147.4 ± 8.6%; n=5, *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.

    Techniques Used: Western Blot, Transfection, Incubation, Staining, Molecular Weight

    anti kir4 1 guinea pig polyclonal antibody  (Alomone Labs)


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    Alomone Labs anti kir4 1 guinea pig polyclonal antibody
    A. Protein levels of <t>Kir4.1</t> measured by Western blot in astrocytes grown in DMEM containing normal glucose and transfected with miRNA-205 mimic (mimic 205) were significantly down-regulated compared to mock-transfected astrocytes (61.4 ± 6.62%; n=5; *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.
    Anti Kir4 1 Guinea Pig Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir4 1 guinea pig polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir4 1 guinea pig polyclonal antibody - by Bioz Stars, 2023-01
    93/100 stars

    Images

    1) Product Images from "Kir4.1 potassium channel regulation via miR-205 in astrocytes exposed to hyperglycemic conditions."

    Article Title: Kir4.1 potassium channel regulation via miR-205 in astrocytes exposed to hyperglycemic conditions.

    Journal: Neuroreport

    doi: 10.1097/WNR.0000000000001427

    A. Protein levels of Kir4.1 measured by Western blot in astrocytes grown in DMEM containing normal glucose and transfected with miRNA-205 mimic (mimic 205) were significantly down-regulated compared to mock-transfected astrocytes (61.4 ± 6.62%; n=5; *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.
    Figure Legend Snippet: A. Protein levels of Kir4.1 measured by Western blot in astrocytes grown in DMEM containing normal glucose and transfected with miRNA-205 mimic (mimic 205) were significantly down-regulated compared to mock-transfected astrocytes (61.4 ± 6.62%; n=5; *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.

    Techniques Used: Western Blot, Transfection, Incubation, Staining, Molecular Weight

    A. Proteins levels of Kir.4.1 measured by Western blot in astrocytes grown in DMEM containing high glucose and transfected with miRNA-205 inhibitor (Inhibitor 205) were significantly up-regulated compare to mock-transfected astrocytes (147.4 ± 8.6%; n=5, *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.
    Figure Legend Snippet: A. Proteins levels of Kir.4.1 measured by Western blot in astrocytes grown in DMEM containing high glucose and transfected with miRNA-205 inhibitor (Inhibitor 205) were significantly up-regulated compare to mock-transfected astrocytes (147.4 ± 8.6%; n=5, *p<0.05). B. Representative picture of the membrane blotting after Kir4.1 antibody incubation with is corresponding India ink stained membrane for loading control calculations. In B the molecular weight of Kir4.1 was detected at ~43kDa.

    Techniques Used: Western Blot, Transfection, Incubation, Staining, Molecular Weight

    anti kir4 1 guinea pig polyclonal antibody  (Alomone Labs)


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    Alomone Labs anti kir4 1 guinea pig polyclonal antibody
    Anti Kir4 1 Guinea Pig Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir4 1 guinea pig polyclonal antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir4 1 guinea pig polyclonal antibody - by Bioz Stars, 2023-01
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    guinea pig polyclonal antibody against kir4 1  (Alomone Labs)


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    Alomone Labs guinea pig polyclonal antibody against kir4 1
    <t>Kir4.1</t> potassium channel protein levels are lower in the hippocampal region of brains from diabetic mice as compared with non-diabetic control mice. ( A ) Kir4.1 potassium channel protein levels (total monomer of both glycosylated and unglycosylated forms of Kir4.1) measured by Western Blot in hippocampus from diabetic mice were significantly downregulated (68.3 ± 2.6%, n = 3) as compared to control. Data are expressed as % of control with control being hippocampus from non-diabetic mice and * indicating a significant difference from control group ( p < 0.05; Student’s t -Test for independent samples). Above the graph in ( A ) are representative bands from the Western blot showing Kir4.1 protein levels from a control and a diabetic mouse. The band detected at ~37 kDa corresponds to the unglycosylated form, whereas the band detected at ~43 corresponds to the glycosylated form of the Kir4.1 monomer. ( B ) The India ink-stained membrane showing the total protein of the samples shown in ( A ) that was used for loading control calculations.
    Guinea Pig Polyclonal Antibody Against Kir4 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig polyclonal antibody against kir4 1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig polyclonal antibody against kir4 1 - by Bioz Stars, 2023-01
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    Images

    1) Product Images from "Downregulation of Astrocytic Kir4.1 Potassium Channels Is Associated with Hippocampal Neuronal Hyperexcitability in Type 2 Diabetic Mice"

    Article Title: Downregulation of Astrocytic Kir4.1 Potassium Channels Is Associated with Hippocampal Neuronal Hyperexcitability in Type 2 Diabetic Mice

    Journal: Brain Sciences

    doi: 10.3390/brainsci10020072

    Kir4.1 potassium channel protein levels are lower in the hippocampal region of brains from diabetic mice as compared with non-diabetic control mice. ( A ) Kir4.1 potassium channel protein levels (total monomer of both glycosylated and unglycosylated forms of Kir4.1) measured by Western Blot in hippocampus from diabetic mice were significantly downregulated (68.3 ± 2.6%, n = 3) as compared to control. Data are expressed as % of control with control being hippocampus from non-diabetic mice and * indicating a significant difference from control group ( p < 0.05; Student’s t -Test for independent samples). Above the graph in ( A ) are representative bands from the Western blot showing Kir4.1 protein levels from a control and a diabetic mouse. The band detected at ~37 kDa corresponds to the unglycosylated form, whereas the band detected at ~43 corresponds to the glycosylated form of the Kir4.1 monomer. ( B ) The India ink-stained membrane showing the total protein of the samples shown in ( A ) that was used for loading control calculations.
    Figure Legend Snippet: Kir4.1 potassium channel protein levels are lower in the hippocampal region of brains from diabetic mice as compared with non-diabetic control mice. ( A ) Kir4.1 potassium channel protein levels (total monomer of both glycosylated and unglycosylated forms of Kir4.1) measured by Western Blot in hippocampus from diabetic mice were significantly downregulated (68.3 ± 2.6%, n = 3) as compared to control. Data are expressed as % of control with control being hippocampus from non-diabetic mice and * indicating a significant difference from control group ( p < 0.05; Student’s t -Test for independent samples). Above the graph in ( A ) are representative bands from the Western blot showing Kir4.1 protein levels from a control and a diabetic mouse. The band detected at ~37 kDa corresponds to the unglycosylated form, whereas the band detected at ~43 corresponds to the glycosylated form of the Kir4.1 monomer. ( B ) The India ink-stained membrane showing the total protein of the samples shown in ( A ) that was used for loading control calculations.

    Techniques Used: Western Blot, Staining

    guinea pig polyclonal  (Alomone Labs)


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    Alomone Labs guinea pig polyclonal
    Guinea Pig Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig polyclonal/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig polyclonal - by Bioz Stars, 2023-01
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    guinea pig  (Alomone Labs)


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  • 93

    Structured Review

    Alomone Labs guinea pig
    Guinea Pig, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig - by Bioz Stars, 2023-01
    93/100 stars

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    guinea pig  (Alomone Labs)


    Bioz Verified Symbol Alomone Labs is a verified supplier
    Bioz Manufacturer Symbol Alomone Labs manufactures this product  
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  • 93

    Structured Review

    Alomone Labs guinea pig
    Guinea Pig, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    guinea pig - by Bioz Stars, 2023-01
    93/100 stars

    Images