α1i  (Alomone Labs)


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    Structured Review

    Alomone Labs α1i
    α1i, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1i/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    α1i - by Bioz Stars, 2022-11
    94/100 stars

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    Alomone Labs anti cav3 3 cacna1i antibody
    ChC axonal arborization requires <t>Cav3.3-T-type</t> VDCC genes in a dosage sensitive manner. ( A and B ) Confocal images of EP13 transplanted ZsGreen-labeled ChCs transfected with LacZ sgRNAs (A, left) or Cav3.3 sgRNAs (A, right) as well as EP13 transplanted GFP-labeled ChCs originating from Cav3.3 germline KO mice (B). Single optical sections indicating synaptic cartridges apposed to AnkG-labeled AISs are shown in the middle panels. The areas in white boxes with arrowheads are enlarged and overlaid with the AnkG channel. Bottom panels indicate reconstructed axonal arbors in the area defined in Fig. 4A . ( C ) The number of branch points in LacZ sgRNA –transfected ChCs ( n = 5 cells from three mice), Cav3.3 sgRNA– transfected homozygous KO ChCs ( n = 5 cells from three mice), transplanted ChCs originating from Cav3.3 homozygous KO mice ( n = 6 cells from three mice), and transplanted ChCs originating from Cav3.3 heterozygous KO mice ( n = 5 cells from three mice). One-way ANOVA, * P
    Anti Cav3 3 Cacna1i Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav3 3 cacna1i antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav3 3 cacna1i antibody - by Bioz Stars, 2022-11
    94/100 stars
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    ChC axonal arborization requires Cav3.3-T-type VDCC genes in a dosage sensitive manner. ( A and B ) Confocal images of EP13 transplanted ZsGreen-labeled ChCs transfected with LacZ sgRNAs (A, left) or Cav3.3 sgRNAs (A, right) as well as EP13 transplanted GFP-labeled ChCs originating from Cav3.3 germline KO mice (B). Single optical sections indicating synaptic cartridges apposed to AnkG-labeled AISs are shown in the middle panels. The areas in white boxes with arrowheads are enlarged and overlaid with the AnkG channel. Bottom panels indicate reconstructed axonal arbors in the area defined in Fig. 4A . ( C ) The number of branch points in LacZ sgRNA –transfected ChCs ( n = 5 cells from three mice), Cav3.3 sgRNA– transfected homozygous KO ChCs ( n = 5 cells from three mice), transplanted ChCs originating from Cav3.3 homozygous KO mice ( n = 6 cells from three mice), and transplanted ChCs originating from Cav3.3 heterozygous KO mice ( n = 5 cells from three mice). One-way ANOVA, * P

    Journal: Science Advances

    Article Title: Neuromodulatory control of inhibitory network arborization in the developing postnatal neocortex

    doi: 10.1126/sciadv.abe7192

    Figure Lengend Snippet: ChC axonal arborization requires Cav3.3-T-type VDCC genes in a dosage sensitive manner. ( A and B ) Confocal images of EP13 transplanted ZsGreen-labeled ChCs transfected with LacZ sgRNAs (A, left) or Cav3.3 sgRNAs (A, right) as well as EP13 transplanted GFP-labeled ChCs originating from Cav3.3 germline KO mice (B). Single optical sections indicating synaptic cartridges apposed to AnkG-labeled AISs are shown in the middle panels. The areas in white boxes with arrowheads are enlarged and overlaid with the AnkG channel. Bottom panels indicate reconstructed axonal arbors in the area defined in Fig. 4A . ( C ) The number of branch points in LacZ sgRNA –transfected ChCs ( n = 5 cells from three mice), Cav3.3 sgRNA– transfected homozygous KO ChCs ( n = 5 cells from three mice), transplanted ChCs originating from Cav3.3 homozygous KO mice ( n = 6 cells from three mice), and transplanted ChCs originating from Cav3.3 heterozygous KO mice ( n = 5 cells from three mice). One-way ANOVA, * P

    Article Snippet: The following primary antibodies were used: goat anti-ChAT (1:200; Millipore, catalog no. AB144P), rabbit anti-Cav3.1 (1:100; Alomone Labs, catalog no.ACC-021), rabbit anti-Cav3.2 (1:100; Alomone Labs, catalog no. ACC-025), rabbit anti-Cav3.3 (1:100; Alomone Labs, catalog no. ACC-009), guinea pig polyclonal anti-PV (1:2000; Swant, PVG-213), rat anti-hemagglutinin (1:500; Roche, catalog no.11-867-423-001), chicken anti-GFP (1:800; Abcam, catalog no.ab13970), mouse anti-AnkG (1:500; UC Davis/NIH Neuromab, catalog no. clone N106/36 75-146), rabbit anti-ZsGreen (1:500; Clontech, catalog no. 632474), and rabbit anti-RFP (1:800; Rockland, catalog no. 600-401-379).

    Techniques: Labeling, Transfection, Mouse Assay

    Hypofunction of T-Ca 2+ channels and decreased CaV3.3 expression in TRN neurons of adult Gclm -KO mice, resulting in alteration of their bursting profile. A Smaller proportion of TRN neurons generating burst firing at resting membrane potential (RMP) in KO as compared to WT mice (WT n = 13; KO n = 12; Fisher exact test, p = 0.02). B Weaker density of T-Ca 2+ currents activated at RMP in TRN neurons of KO ( n = 11) as compared to WT ( n = 10) mice ( p = 0.034, one-tailed t -test). C Representative recording of single bursting in a TRN neuron. D Threshold for the initial membrane potential required to induce single bursting upon depolarization. E Representative recording of repetitive bursting in a TRN neuron. F Threshold for the initial membrane potential required to induce repetitive bursting upon depolarization. Note that KO ( n = 6) TRN neurons require a more hyperpolarized membrane potential, particularly for exhibiting repetitive bursts, as compared to WT mice ( n = 8). G , H Top: Representative recordings of T-Ca 2+ and SK currents induced by a short constant depolarization step (from −110 to −40 mV), with their amplitudes increasing with greater hyperpolarizing initial membrane potential (going from −30 mV to −110 mV). Bottom: Density of T-Ca 2+ ( G ) and SK currents ( H ) activated from each of the initial membrane potentials. Compared to WT mice, TRN neurons in KO display overall smaller T-Ca 2+ ( F = 31.92 DF n = 1 DFd = 289; p

    Journal: Molecular Psychiatry

    Article Title: Developmental oxidative stress leads to T-type Ca2+ channel hypofunction in thalamic reticular nucleus of mouse models pertinent to schizophrenia

    doi: 10.1038/s41380-021-01425-2

    Figure Lengend Snippet: Hypofunction of T-Ca 2+ channels and decreased CaV3.3 expression in TRN neurons of adult Gclm -KO mice, resulting in alteration of their bursting profile. A Smaller proportion of TRN neurons generating burst firing at resting membrane potential (RMP) in KO as compared to WT mice (WT n = 13; KO n = 12; Fisher exact test, p = 0.02). B Weaker density of T-Ca 2+ currents activated at RMP in TRN neurons of KO ( n = 11) as compared to WT ( n = 10) mice ( p = 0.034, one-tailed t -test). C Representative recording of single bursting in a TRN neuron. D Threshold for the initial membrane potential required to induce single bursting upon depolarization. E Representative recording of repetitive bursting in a TRN neuron. F Threshold for the initial membrane potential required to induce repetitive bursting upon depolarization. Note that KO ( n = 6) TRN neurons require a more hyperpolarized membrane potential, particularly for exhibiting repetitive bursts, as compared to WT mice ( n = 8). G , H Top: Representative recordings of T-Ca 2+ and SK currents induced by a short constant depolarization step (from −110 to −40 mV), with their amplitudes increasing with greater hyperpolarizing initial membrane potential (going from −30 mV to −110 mV). Bottom: Density of T-Ca 2+ ( G ) and SK currents ( H ) activated from each of the initial membrane potentials. Compared to WT mice, TRN neurons in KO display overall smaller T-Ca 2+ ( F = 31.92 DF n = 1 DFd = 289; p

    Article Snippet: We carried out CaV3.2/PV and CaV3.3/PV immunohistology as indicated in using the following primary [rabbit anti-CaV3.2 (Santa Cruz Biotechnology, USA); rabbit anti-CaV3.3 (Alomone labs, Israel), sheep anti-PV (R & D systems)] and secondary [goat anti-rabbit AF488 and donkey anti-sheep AF594 (Abcam, UK)] antibodies.

    Techniques: Expressing, Mouse Assay, One-tailed Test