α1i  (Alomone Labs)


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    Structured Review

    Alomone Labs α1i
    α1i, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α1i/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α1i - by Bioz Stars, 2022-07
    94/100 stars

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    Alomone Labs anti cav3 3 cacna1i antibody
    Low-voltage activated calcium channel family (Cav3) subunits in the abducens and trochlear nucleus. a Consecutive coronal paraffin sections through the abducens nucleus (nVI) ( a ) depicting the immunolabeling for Cav3.1 (first panel), Cav3.2 (third panel) and <t>Cav3.3</t> (fourth panel) subunits with combined immunostaining for ChAT (brown) and ACAN (black) (second panel) as reference. b, c Close-up of Cav subunit expression in nVI MIF (red arrowheads) and SIF motoneurons (MNs) (green arrows) and INTs (blue arrow). d Consecutive coronal paraffin sections through the trochlear nucleus (nVI) depicting the immunolabeling for Cav3.1 (first panel), Cav3.2 (second panel) and Cav3.3 (fourth panel) subunits with combined immunostaining for ChAT (brown) and ACAN (black) (third panel) as reference. Thin dashed lines in d indicate the border of nIV and thick dashed lines indicate the boundary between the MIF and SIF MNs. e Close-up of Cav subunit expression in MIF (red arrowheads) and SIF MNs (green arrow) on different sections as those illustrated in d . Note the weak Cav3.1 expression along the membrane of some SIF MNs (left column, black star). Red dashed lines indicate the tentative position of the border delineating the dorsal cap of nIV. f Cerebellar Purkinje cells located on the same consecutive sections as nVI as controls for immunopositivity of the different Cav subunits. Scale bar indicates 100 μm in a, d, f and 50 μm in b, c, e
    Anti Cav3 3 Cacna1i Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav3 3 cacna1i antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav3 3 cacna1i antibody - by Bioz Stars, 2022-07
    94/100 stars
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    Low-voltage activated calcium channel family (Cav3) subunits in the abducens and trochlear nucleus. a Consecutive coronal paraffin sections through the abducens nucleus (nVI) ( a ) depicting the immunolabeling for Cav3.1 (first panel), Cav3.2 (third panel) and Cav3.3 (fourth panel) subunits with combined immunostaining for ChAT (brown) and ACAN (black) (second panel) as reference. b, c Close-up of Cav subunit expression in nVI MIF (red arrowheads) and SIF motoneurons (MNs) (green arrows) and INTs (blue arrow). d Consecutive coronal paraffin sections through the trochlear nucleus (nVI) depicting the immunolabeling for Cav3.1 (first panel), Cav3.2 (second panel) and Cav3.3 (fourth panel) subunits with combined immunostaining for ChAT (brown) and ACAN (black) (third panel) as reference. Thin dashed lines in d indicate the border of nIV and thick dashed lines indicate the boundary between the MIF and SIF MNs. e Close-up of Cav subunit expression in MIF (red arrowheads) and SIF MNs (green arrow) on different sections as those illustrated in d . Note the weak Cav3.1 expression along the membrane of some SIF MNs (left column, black star). Red dashed lines indicate the tentative position of the border delineating the dorsal cap of nIV. f Cerebellar Purkinje cells located on the same consecutive sections as nVI as controls for immunopositivity of the different Cav subunits. Scale bar indicates 100 μm in a, d, f and 50 μm in b, c, e

    Journal: Brain Structure & Function

    Article Title: Transmitter and ion channel profiles of neurons in the primate abducens and trochlear nuclei

    doi: 10.1007/s00429-021-02315-7

    Figure Lengend Snippet: Low-voltage activated calcium channel family (Cav3) subunits in the abducens and trochlear nucleus. a Consecutive coronal paraffin sections through the abducens nucleus (nVI) ( a ) depicting the immunolabeling for Cav3.1 (first panel), Cav3.2 (third panel) and Cav3.3 (fourth panel) subunits with combined immunostaining for ChAT (brown) and ACAN (black) (second panel) as reference. b, c Close-up of Cav subunit expression in nVI MIF (red arrowheads) and SIF motoneurons (MNs) (green arrows) and INTs (blue arrow). d Consecutive coronal paraffin sections through the trochlear nucleus (nVI) depicting the immunolabeling for Cav3.1 (first panel), Cav3.2 (second panel) and Cav3.3 (fourth panel) subunits with combined immunostaining for ChAT (brown) and ACAN (black) (third panel) as reference. Thin dashed lines in d indicate the border of nIV and thick dashed lines indicate the boundary between the MIF and SIF MNs. e Close-up of Cav subunit expression in MIF (red arrowheads) and SIF MNs (green arrow) on different sections as those illustrated in d . Note the weak Cav3.1 expression along the membrane of some SIF MNs (left column, black star). Red dashed lines indicate the tentative position of the border delineating the dorsal cap of nIV. f Cerebellar Purkinje cells located on the same consecutive sections as nVI as controls for immunopositivity of the different Cav subunits. Scale bar indicates 100 μm in a, d, f and 50 μm in b, c, e

    Article Snippet: Voltage-dependent T-type calcium channel subunits Cav3.1 (CACNA1G, α1G; Cat #: ACC-021; RRID: AB_2039779), Cav3.2 (CACNA1H, α1H; Cat #: ACC-025; RRID: AB_2039781) and Cav3.3 (CACNA1I, α1H; Cat #: ACC-009; RRID: AB_2039783) were detected with polyclonal rabbit antibodies from (Alomone Labs, Jerusalem, ISRAEL).

    Techniques: Immunolabeling, Immunostaining, Expressing

    ChC axonal arborization requires Cav3.3-T-type VDCC genes in a dosage sensitive manner. ( A and B ) Confocal images of EP13 transplanted ZsGreen-labeled ChCs transfected with LacZ sgRNAs (A, left) or Cav3.3 sgRNAs (A, right) as well as EP13 transplanted GFP-labeled ChCs originating from Cav3.3 germline KO mice (B). Single optical sections indicating synaptic cartridges apposed to AnkG-labeled AISs are shown in the middle panels. The areas in white boxes with arrowheads are enlarged and overlaid with the AnkG channel. Bottom panels indicate reconstructed axonal arbors in the area defined in Fig. 4A . ( C ) The number of branch points in LacZ sgRNA –transfected ChCs ( n = 5 cells from three mice), Cav3.3 sgRNA– transfected homozygous KO ChCs ( n = 5 cells from three mice), transplanted ChCs originating from Cav3.3 homozygous KO mice ( n = 6 cells from three mice), and transplanted ChCs originating from Cav3.3 heterozygous KO mice ( n = 5 cells from three mice). One-way ANOVA, * P

    Journal: Science Advances

    Article Title: Neuromodulatory control of inhibitory network arborization in the developing postnatal neocortex

    doi: 10.1126/sciadv.abe7192

    Figure Lengend Snippet: ChC axonal arborization requires Cav3.3-T-type VDCC genes in a dosage sensitive manner. ( A and B ) Confocal images of EP13 transplanted ZsGreen-labeled ChCs transfected with LacZ sgRNAs (A, left) or Cav3.3 sgRNAs (A, right) as well as EP13 transplanted GFP-labeled ChCs originating from Cav3.3 germline KO mice (B). Single optical sections indicating synaptic cartridges apposed to AnkG-labeled AISs are shown in the middle panels. The areas in white boxes with arrowheads are enlarged and overlaid with the AnkG channel. Bottom panels indicate reconstructed axonal arbors in the area defined in Fig. 4A . ( C ) The number of branch points in LacZ sgRNA –transfected ChCs ( n = 5 cells from three mice), Cav3.3 sgRNA– transfected homozygous KO ChCs ( n = 5 cells from three mice), transplanted ChCs originating from Cav3.3 homozygous KO mice ( n = 6 cells from three mice), and transplanted ChCs originating from Cav3.3 heterozygous KO mice ( n = 5 cells from three mice). One-way ANOVA, * P

    Article Snippet: The following primary antibodies were used: goat anti-ChAT (1:200; Millipore, catalog no. AB144P), rabbit anti-Cav3.1 (1:100; Alomone Labs, catalog no.ACC-021), rabbit anti-Cav3.2 (1:100; Alomone Labs, catalog no. ACC-025), rabbit anti-Cav3.3 (1:100; Alomone Labs, catalog no. ACC-009), guinea pig polyclonal anti-PV (1:2000; Swant, PVG-213), rat anti-hemagglutinin (1:500; Roche, catalog no.11-867-423-001), chicken anti-GFP (1:800; Abcam, catalog no.ab13970), mouse anti-AnkG (1:500; UC Davis/NIH Neuromab, catalog no. clone N106/36 75-146), rabbit anti-ZsGreen (1:500; Clontech, catalog no. 632474), and rabbit anti-RFP (1:800; Rockland, catalog no. 600-401-379).

    Techniques: Labeling, Transfection, Mouse Assay

    Activation of NmbR stimulates recombinant Cav3.2 channels heterologously expressed in HEK293 cells. A, western blot analysis of NmbR in HEK293 cells transiently transfected with NmbR cDNA. Blots depicted are representative of three independent experiments. B, membrane localization of NmbR in transfected HEK293 cells. Alphabets a through c in the diagram indicate the differential interference contrast (DIC, a ), the EGFP fluorescent signals of NmbR ( b ), and the merged image ( c ), respectively. C, exemplary current traces show the effect of Nmb (100 nM) on Cav3.1 (α1G), Cav3.2 (α1H) and Cav3.3 (α1I) channel currents. Currents were elicited by a 100 ms depolarizing step pulse from the holding potential of -110 mV to -30 mV. D, summary data show the effect of 100 nM Nmb on Cav3.1 ( n = 10), Cav3.2 ( n = 9) and Cav3.3 ( n = 7) channel currents. ** p

    Journal: Theranostics

    Article Title: Neuromedin B receptor stimulation of Cav3.2 T-type Ca2+ channels in primary sensory neurons mediates peripheral pain hypersensitivity

    doi: 10.7150/thno.62255

    Figure Lengend Snippet: Activation of NmbR stimulates recombinant Cav3.2 channels heterologously expressed in HEK293 cells. A, western blot analysis of NmbR in HEK293 cells transiently transfected with NmbR cDNA. Blots depicted are representative of three independent experiments. B, membrane localization of NmbR in transfected HEK293 cells. Alphabets a through c in the diagram indicate the differential interference contrast (DIC, a ), the EGFP fluorescent signals of NmbR ( b ), and the merged image ( c ), respectively. C, exemplary current traces show the effect of Nmb (100 nM) on Cav3.1 (α1G), Cav3.2 (α1H) and Cav3.3 (α1I) channel currents. Currents were elicited by a 100 ms depolarizing step pulse from the holding potential of -110 mV to -30 mV. D, summary data show the effect of 100 nM Nmb on Cav3.1 ( n = 10), Cav3.2 ( n = 9) and Cav3.3 ( n = 7) channel currents. ** p

    Article Snippet: The membranes were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) and probed with antibodies against Nmb (rabbit, 1:1000, Abcam, Cat. No. ab191499), Cav3.1 (rabbit, 1:1000, Alomone, Cat. No. ACC-021), Cav3.2 (mouse, 1:1000, Novus, Cat. No. NBP1-22444), Cav3.3 (rabbit, 1:1000, Alomone, Cat. No. ACC-009), NmbR (rabbit, 1:500, Sigma, Cat. No. SAB4502914), phospho-AMPKα1 (rabbit, 1:500, Abcam, Cat. No. ab92701), AMPKα1 (rabbit, 1:600, Abcam, Cat. No. ab110036), Gαq/11 (mouse, 1:600, Santa Cruz Biotechnology, Cat. No. SC-365906), and Gβ (mouse, 1:500, Santa Cruz Biotechnology, Cat. No. SC-515822).

    Techniques: Activation Assay, Recombinant, Western Blot, Transfection

    In vivo and in vitro expression of Cav3.3: Immunostaining of Cav3.3 in dissociated PCs at 2div ( A – D ), and in cryosections of rat cerebella at p0 ( E – H ) and p9 ( I – L ). Cav3.3 was stained green, calbindin red, and nuclear staining was performed with Hoechst (blue). In the PC culture, Cav3.3 was localized at the soma and along the dendrites or axons, whereas in cryosections of p0, cerebella staining of Cav3.3 was visible mainly near to the soma. In 9-day-old PCs, the Cav3.3-antibody strongly marked the ML, where the PC dendrites are localized. eGCL = external granular cell layer, ML = molecular layer, PCL = PC layer, iGCL = internal granular cell layer. Scale bar: 20 µm ( A , B , E , F , I , J ) and 2 µm ( C , D , G , H , K , L ).

    Journal: Cells

    Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

    doi: 10.3390/cells10092277

    Figure Lengend Snippet: In vivo and in vitro expression of Cav3.3: Immunostaining of Cav3.3 in dissociated PCs at 2div ( A – D ), and in cryosections of rat cerebella at p0 ( E – H ) and p9 ( I – L ). Cav3.3 was stained green, calbindin red, and nuclear staining was performed with Hoechst (blue). In the PC culture, Cav3.3 was localized at the soma and along the dendrites or axons, whereas in cryosections of p0, cerebella staining of Cav3.3 was visible mainly near to the soma. In 9-day-old PCs, the Cav3.3-antibody strongly marked the ML, where the PC dendrites are localized. eGCL = external granular cell layer, ML = molecular layer, PCL = PC layer, iGCL = internal granular cell layer. Scale bar: 20 µm ( A , B , E , F , I , J ) and 2 µm ( C , D , G , H , K , L ).

    Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.

    Techniques: In Vivo, In Vitro, Expressing, Immunostaining, Staining

    In vivo expression of Cacna1g , Cacna1h , and Cacna1i : Examination of the relative mRNA expression pattern of Cacna1g , Cacna1h , and Cacna1i in PCs was performed at p9 and p30 via qPCR of LMD samples. The values were normalized against the values of the PCs of p9, and Gapdh was the housekeeping gene. Note that 1,000,000 μm 2 of enriched PCs from 20 different rat cerebella were collected. The analysis was performed with the help of the 2 −ΔΔct method. Significant differences were indicated by *** p

    Journal: Cells

    Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

    doi: 10.3390/cells10092277

    Figure Lengend Snippet: In vivo expression of Cacna1g , Cacna1h , and Cacna1i : Examination of the relative mRNA expression pattern of Cacna1g , Cacna1h , and Cacna1i in PCs was performed at p9 and p30 via qPCR of LMD samples. The values were normalized against the values of the PCs of p9, and Gapdh was the housekeeping gene. Note that 1,000,000 μm 2 of enriched PCs from 20 different rat cerebella were collected. The analysis was performed with the help of the 2 −ΔΔct method. Significant differences were indicated by *** p

    Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.

    Techniques: In Vivo, Expressing, Real-time Polymerase Chain Reaction, Laser Capture Microdissection

    Effect of VEGF on Cacna1g , Cacna1h , and Cacna1i expression: The graphs show the relative mRNA expression of Cacna1g , Cacna1h , and Cacna1i with or without VEGF treatment for 24 h and 48 h. The values were normalised against the control group and the housekeeping gene was Gapdh . n = 8, significant differences are indicated by * p

    Journal: Cells

    Article Title: Expression Pattern of T-Type Ca2+ Channels in Cerebellar Purkinje Cells after VEGF Treatment

    doi: 10.3390/cells10092277

    Figure Lengend Snippet: Effect of VEGF on Cacna1g , Cacna1h , and Cacna1i expression: The graphs show the relative mRNA expression of Cacna1g , Cacna1h , and Cacna1i with or without VEGF treatment for 24 h and 48 h. The values were normalised against the control group and the housekeeping gene was Gapdh . n = 8, significant differences are indicated by * p

    Article Snippet: On the next day, the samples were washed with PBS three times for 10 min and incubated with secondary anti-mouse TRITC antibody (goat, 1:1000 T5393, Sigma-Aldrich) at room temperature for 2 h, respectively, 2.5 h. After additional washing, the second primary antibody anti-Cav3.1 (rabbit, 1:1500 ACC21, Alomone Labs), anti-Cav3.2 (Rabbit, 1:1500 ACC25, Alomone Labs) or anti-Cav3.3 (rabbit, 1:1000 ACC-009, Alomone Labs) was applied at 4 °C overnight.

    Techniques: Expressing