rabbit anti ca v 1 2 polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit anti ca v 1 2 polyclonal antibody
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Rabbit Anti Ca V 1 2 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ca v 1 2 polyclonal antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti ca v 1 2 polyclonal antibody - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart"

    Article Title: The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074719

    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Figure Legend Snippet: A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..

    Techniques Used: Western Blot


    Figure Legend Snippet: Comparison of Key Ca 2+ Handling Mechanisms in Hearts and Cardiomyocytes From Young Adult OVX and Aged OVX Female Mice.

    Techniques Used: In Vivo, Activity Assay, Expressing

    rabbit anti ca v 1 2 polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit anti ca v 1 2 polyclonal antibody
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Rabbit Anti Ca V 1 2 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ca v 1 2 polyclonal antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti ca v 1 2 polyclonal antibody - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart"

    Article Title: The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074719

    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Figure Legend Snippet: A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..

    Techniques Used: Western Blot


    Figure Legend Snippet: Comparison of Key Ca 2+ Handling Mechanisms in Hearts and Cardiomyocytes From Young Adult OVX and Aged OVX Female Mice.

    Techniques Used: In Vivo, Activity Assay, Expressing

    cav1 2 rabbit polyclonal  (Alomone Labs)


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    Alomone Labs cav1 2 rabbit polyclonal
    Cav1 2 Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acc 003 ag  (Alomone Labs)


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    Alomone Labs acc 003 ag
    Acc 003 Ag, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acc 003 ag - by Bioz Stars, 2023-01
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    acc 003 ag  (Alomone Labs)


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    Alomone Labs acc 003 ag
    Acc 003 Ag, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ca 2 channel anti cav1.2  (Alomone Labs)


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    Alomone Labs ca 2 channel anti cav1.2
    Ca 2 Channel Anti Cav1.2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit pab cav1 2  (Alomone Labs)


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    Alomone Labs rabbit pab cav1 2
    Rabbit Pab Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti cav1 2 atto 488  (Alomone Labs)


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    Alomone Labs anti cav1 2 atto 488
    Anti Cav1 2 Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti cav1 2 a1c l channel  (Alomone Labs)


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    Alomone Labs anti cav1 2 a1c l channel
    Anti Cav1 2 A1c L Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbs  (Alomone Labs)


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    Alomone Labs pbs
    Pbs, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    affinity purified rabbit antibody  (Alomone Labs)


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    Alomone Labs affinity purified rabbit antibody
    Affinity Purified Rabbit Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    • rabbit anti ca v 1 2 polyclonal antibody  (Alomone Labs)
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    Alomone Labs rabbit anti ca v 1 2 polyclonal antibody
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Rabbit Anti Ca V 1 2 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ca v 1 2 polyclonal antibody/product/Alomone Labs
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    rabbit anti ca v 1 2 polyclonal antibody - by Bioz Stars, 2023-01
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    cav1 2 rabbit polyclonal  (Alomone Labs)
    94
    Alomone Labs cav1 2 rabbit polyclonal
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Cav1 2 Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acc 003 ag  (Alomone Labs)
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    Alomone Labs acc 003 ag
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
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    ca 2 channel anti cav1.2  (Alomone Labs)
    94
    Alomone Labs ca 2 channel anti cav1.2
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
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    rabbit pab cav1 2  (Alomone Labs)
    94
    Alomone Labs rabbit pab cav1 2
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
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    94
    Alomone Labs anti cav1 2 atto 488
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Anti Cav1 2 Atto 488, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti cav1 2 a1c l channel  (Alomone Labs)
    94
    Alomone Labs anti cav1 2 a1c l channel
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Anti Cav1 2 A1c L Channel, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbs  (Alomone Labs)
    94
    Alomone Labs pbs
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Pbs, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    affinity purified rabbit antibody  (Alomone Labs)
    94
    Alomone Labs affinity purified rabbit antibody
    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..
    Affinity Purified Rabbit Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified rabbit antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity purified rabbit antibody - by Bioz Stars, 2023-01
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..

    Journal: PLoS ONE

    Article Title: The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart

    doi: 10.1371/journal.pone.0074719

    Figure Lengend Snippet: A . Detection of ~240 kDa bands corresponding to Ca V 1.2 in ventricular tissue from sham and OVX mice. Na/K ATPase (110 kDa band) was used as a loading control (15 µg of protein were loaded in each lane). Average Ca V 1.2 band intensity was lower in OVX ventricular tissue compared to sham controls (n=3 hearts/group). B . Representative immunoblot illustrating ~116 kDa bands corresponding to NCX in sham and OVX ventricle. The loading control was Na-K ATPase as in A. Mean normalized NCX band intensity was similar in ventricular tissue from sham and OVX mice (n=3 hearts in each group). C . Detection of ~110 kDa bands corresponding to SERCA2 in the ventricles of sham and OVX mice. Amido black was used as a loading control (60 µg of protein were loaded in each lane). Average intensity of the SERCA2 bands was similar in sham and OVX ventricles (n=3 hearts in each group). In experiments where Na-K ATPase was used as a loading control, there was no significant difference in Na-K ATPase protein levels between sham and OVX (t-test, p=0.164) (*denotes p<0.05; t-test)..

    Article Snippet: Antibodies used were rabbit anti- Ca v 1.2 polyclonal antibody (Alomone, ACC-003-AG; 1:2000), mouse anti-NCX monoclonal antibody (SWANT, R3F1; 1:1000), mouse anti-SERCA2 monoclonal antibody (Affinity Bioreagents, MA3-919; 1:2000) and rabbit anti-Na/KATPase polyclonal antibody (Abcam; 1:2000).

    Techniques: Western Blot

    Journal: PLoS ONE

    Article Title: The Impact of Ovariectomy on Calcium Homeostasis and Myofilament Calcium Sensitivity in the Aging Mouse Heart

    doi: 10.1371/journal.pone.0074719

    Figure Lengend Snippet: Comparison of Key Ca 2+ Handling Mechanisms in Hearts and Cardiomyocytes From Young Adult OVX and Aged OVX Female Mice.

    Article Snippet: Antibodies used were rabbit anti- Ca v 1.2 polyclonal antibody (Alomone, ACC-003-AG; 1:2000), mouse anti-NCX monoclonal antibody (SWANT, R3F1; 1:1000), mouse anti-SERCA2 monoclonal antibody (Affinity Bioreagents, MA3-919; 1:2000) and rabbit anti-Na/KATPase polyclonal antibody (Abcam; 1:2000).

    Techniques: In Vivo, Activity Assay, Expressing