cacna1b  (Alomone Labs)


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    Alomone Labs cacna1b
    Cacna1b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cacna1b/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cacna1b - by Bioz Stars, 2022-08
    94/100 stars

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    Alomone Labs cav2 2
    DISC1 regulates <t>Cav2.2-dependent</t> SV exocytosis. (A) Average vGpH traces in scr (101 boutons, 6 fields, 2 exps) and DISC1-E (87 boutons, 5 fields, 2 exps) shRNA-expressing neurons during consecutive trains of APs (300 AP, 10 Hz) in the absence or presence of the Cav2.2 blocker ω-Conotoxin GVIA (125 nM). (B) Boxplot of SV exocytic rates before and after ω-Conotoxin GVIA application. Exocytic rates were measured by linear fitting of the first six time points of the vGpH response. (C) Average vGpH traces in scr (1294 boutons, 9 fields, 3 exps) and DISC1-E (1282 boutons, 9 fields, 3 exps) shRNA-expressing neurons during consecutive trains of APs (300 AP, 10 Hz) in the absence or presence of the Cav2.1 blocker ω-Agatoxin TK (125 nM). (D) Boxplot of SV exocytic rates before and after ω-Agatoxin TK application. (E) Table showing the percentage of inhibition of exocytosis rate by DISC1 knockdown before and after Cav2.2- or Cav2.1 blockade.
    Cav2 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav2 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cav2 2 - by Bioz Stars, 2022-08
    94/100 stars
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    DISC1 regulates Cav2.2-dependent SV exocytosis. (A) Average vGpH traces in scr (101 boutons, 6 fields, 2 exps) and DISC1-E (87 boutons, 5 fields, 2 exps) shRNA-expressing neurons during consecutive trains of APs (300 AP, 10 Hz) in the absence or presence of the Cav2.2 blocker ω-Conotoxin GVIA (125 nM). (B) Boxplot of SV exocytic rates before and after ω-Conotoxin GVIA application. Exocytic rates were measured by linear fitting of the first six time points of the vGpH response. (C) Average vGpH traces in scr (1294 boutons, 9 fields, 3 exps) and DISC1-E (1282 boutons, 9 fields, 3 exps) shRNA-expressing neurons during consecutive trains of APs (300 AP, 10 Hz) in the absence or presence of the Cav2.1 blocker ω-Agatoxin TK (125 nM). (D) Boxplot of SV exocytic rates before and after ω-Agatoxin TK application. (E) Table showing the percentage of inhibition of exocytosis rate by DISC1 knockdown before and after Cav2.2- or Cav2.1 blockade.

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Stimulation of Synaptic Vesicle Exocytosis by the Mental Disease Gene DISC1 is Mediated by N-Type Voltage-Gated Calcium Channels

    doi: 10.3389/fnsyn.2016.00015

    Figure Lengend Snippet: DISC1 regulates Cav2.2-dependent SV exocytosis. (A) Average vGpH traces in scr (101 boutons, 6 fields, 2 exps) and DISC1-E (87 boutons, 5 fields, 2 exps) shRNA-expressing neurons during consecutive trains of APs (300 AP, 10 Hz) in the absence or presence of the Cav2.2 blocker ω-Conotoxin GVIA (125 nM). (B) Boxplot of SV exocytic rates before and after ω-Conotoxin GVIA application. Exocytic rates were measured by linear fitting of the first six time points of the vGpH response. (C) Average vGpH traces in scr (1294 boutons, 9 fields, 3 exps) and DISC1-E (1282 boutons, 9 fields, 3 exps) shRNA-expressing neurons during consecutive trains of APs (300 AP, 10 Hz) in the absence or presence of the Cav2.1 blocker ω-Agatoxin TK (125 nM). (D) Boxplot of SV exocytic rates before and after ω-Agatoxin TK application. (E) Table showing the percentage of inhibition of exocytosis rate by DISC1 knockdown before and after Cav2.2- or Cav2.1 blockade.

    Article Snippet: The rabbit polyclonal Abs against Cav2.1 (#ACC-001) and Cav2.2 (#ACC-002) were from Alomone Labs.

    Techniques: shRNA, Expressing, Inhibition

    DISC1 enhances Cav2.2 and Cav2.1 currents. (A) Western blot showing expression of ectopic (human) DISC1 in HEK293 cells. (B,C) Cav2.2 Current-voltage (I-V) curves for hDISC1-expressing and control cells. (B) Stimulation protocol and individual current responses shown at three different voltages (−30, 0 and 30 mV) (C) . Average I-V plots for hDISC1 (peak = 54.2 ± 4.5 pA/pF, 13 cells) and control (peak = 39.2 ± 4.9 pA/pF, 12 cells), p = 0.033. (D–F) Cav2.2 activation curves in response to the tail protocol. (D) Illustration of the tail protocol and individual tail currents measured at −50 mV after three different voltage steps (−20, 0 and 40 mV). (E) Average current density based on tail currents for DISC1 (164.3 ± 7.3 pA/pF, 12 cells) and control (110.5 ± 6.3 pA/pF, 12 cells), * p

    Journal: Frontiers in Synaptic Neuroscience

    Article Title: Stimulation of Synaptic Vesicle Exocytosis by the Mental Disease Gene DISC1 is Mediated by N-Type Voltage-Gated Calcium Channels

    doi: 10.3389/fnsyn.2016.00015

    Figure Lengend Snippet: DISC1 enhances Cav2.2 and Cav2.1 currents. (A) Western blot showing expression of ectopic (human) DISC1 in HEK293 cells. (B,C) Cav2.2 Current-voltage (I-V) curves for hDISC1-expressing and control cells. (B) Stimulation protocol and individual current responses shown at three different voltages (−30, 0 and 30 mV) (C) . Average I-V plots for hDISC1 (peak = 54.2 ± 4.5 pA/pF, 13 cells) and control (peak = 39.2 ± 4.9 pA/pF, 12 cells), p = 0.033. (D–F) Cav2.2 activation curves in response to the tail protocol. (D) Illustration of the tail protocol and individual tail currents measured at −50 mV after three different voltage steps (−20, 0 and 40 mV). (E) Average current density based on tail currents for DISC1 (164.3 ± 7.3 pA/pF, 12 cells) and control (110.5 ± 6.3 pA/pF, 12 cells), * p

    Article Snippet: The rabbit polyclonal Abs against Cav2.1 (#ACC-001) and Cav2.2 (#ACC-002) were from Alomone Labs.

    Techniques: Western Blot, Expressing, Activation Assay

    Effect of Cav2.2 on rat inflammatory response. (a) ELISA-based measurement of IL-6 and TNF- α expression in rat serum; (b) qRT-PCR-based measurement of mRNA expression of iNOS, MCP-1, and Cav2.2 in rat sciatic nerve; (c) western blot-based measurement of protein expression of iNOS, MCP-1, and Cav2.2 in rat sciatic nerve. ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Upregulation of N-Type Voltage-Gated Calcium Channels Induces Neuropathic Pain in Experimental Autoimmune Neuritis

    doi: 10.1155/2022/8547095

    Figure Lengend Snippet: Effect of Cav2.2 on rat inflammatory response. (a) ELISA-based measurement of IL-6 and TNF- α expression in rat serum; (b) qRT-PCR-based measurement of mRNA expression of iNOS, MCP-1, and Cav2.2 in rat sciatic nerve; (c) western blot-based measurement of protein expression of iNOS, MCP-1, and Cav2.2 in rat sciatic nerve. ∗ P

    Article Snippet: On completion of a 1 h blocking step with 5% skimmed milk at ambient temperature, primary antibodies iNOS (ab49999, Abcam, UK), MCP-1 (ab7202, Abcam, UK), and Cav2.2 (ACC-002, AlomoneLabs, Israel) were served for overnight coincubation with the membrane at 4°C.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

    Effect of Cav2.2 on clinical score and sciatic nerve tissue of rats. (a) Clinical score record of rats during 18 days of immunization; (b) changes in body weight of rats during 18 days of immunization; (c) luxol fast blue (LFB) staining-based observation of the degree of cell infiltration and demyelination in sciatic nerve tissue of rats on the 18 th day of immunization. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Upregulation of N-Type Voltage-Gated Calcium Channels Induces Neuropathic Pain in Experimental Autoimmune Neuritis

    doi: 10.1155/2022/8547095

    Figure Lengend Snippet: Effect of Cav2.2 on clinical score and sciatic nerve tissue of rats. (a) Clinical score record of rats during 18 days of immunization; (b) changes in body weight of rats during 18 days of immunization; (c) luxol fast blue (LFB) staining-based observation of the degree of cell infiltration and demyelination in sciatic nerve tissue of rats on the 18 th day of immunization. ∗∗ P

    Article Snippet: On completion of a 1 h blocking step with 5% skimmed milk at ambient temperature, primary antibodies iNOS (ab49999, Abcam, UK), MCP-1 (ab7202, Abcam, UK), and Cav2.2 (ACC-002, AlomoneLabs, Israel) were served for overnight coincubation with the membrane at 4°C.

    Techniques: Staining

    Effect of Cav2.2 on allodynia in rats. (a–d) Measurement of withdrawal threshold to mechanical (a), thermal (b), and cold (c) stimulation, and mechanical hyperalgesia threshold (d) during 18 days of immunization. ∗∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Upregulation of N-Type Voltage-Gated Calcium Channels Induces Neuropathic Pain in Experimental Autoimmune Neuritis

    doi: 10.1155/2022/8547095

    Figure Lengend Snippet: Effect of Cav2.2 on allodynia in rats. (a–d) Measurement of withdrawal threshold to mechanical (a), thermal (b), and cold (c) stimulation, and mechanical hyperalgesia threshold (d) during 18 days of immunization. ∗∗ P

    Article Snippet: On completion of a 1 h blocking step with 5% skimmed milk at ambient temperature, primary antibodies iNOS (ab49999, Abcam, UK), MCP-1 (ab7202, Abcam, UK), and Cav2.2 (ACC-002, AlomoneLabs, Israel) were served for overnight coincubation with the membrane at 4°C.

    Techniques: