Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Upregulation of N-Type Voltage-Gated Calcium Channels Induces Neuropathic Pain in Experimental Autoimmune Neuritis
Figure Lengend Snippet: Effect of Cav2.2 on rat inflammatory response. (a) ELISA-based measurement of IL-6 and TNF- α expression in rat serum; (b) qRT-PCR-based measurement of mRNA expression of iNOS, MCP-1, and Cav2.2 in rat sciatic nerve; (c) western blot-based measurement of protein expression of iNOS, MCP-1, and Cav2.2 in rat sciatic nerve. ∗ P
Article Snippet: On completion of a 1 h blocking step with 5% skimmed milk at ambient temperature, primary antibodies iNOS (ab49999, Abcam, UK), MCP-1 (ab7202, Abcam, UK), and Cav2.2 (ACC-002, AlomoneLabs, Israel) were served for overnight coincubation with the membrane at 4°C.
Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot