anti bestrophin 1 extracellular antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti bestrophin 1 extracellular antibody
    <t>Bestrophin-1</t> is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 <t>(Best-1)</t> at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or <t>antibodies,</t> respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Anti Bestrophin 1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bestrophin 1 extracellular antibody/product/Alomone Labs
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti bestrophin 1 extracellular antibody - by Bioz Stars, 2022-10
    85/100 stars

    Images

    1) Product Images from "Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats"

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    Journal: Molecular Pain

    doi: 10.1186/s12990-015-0042-1

    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Figure Legend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p
    Figure Legend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Techniques Used: Expressing, Ligation

    2) Product Images from "Chloride channels mediate sodium sulphide-induced relaxation in rat uteri"

    Article Title: Chloride channels mediate sodium sulphide-induced relaxation in rat uteri

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.13161

    (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass
    Figure Legend Snippet: (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

    3) Product Images from "Chloride channels mediate sodium sulphide-induced relaxation in rat uteri"

    Article Title: Chloride channels mediate sodium sulphide-induced relaxation in rat uteri

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.13161

    (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass
    Figure Legend Snippet: (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot

    4) Product Images from "Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats"

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    Journal: Molecular Pain

    doi: 10.1186/s12990-015-0042-1

    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Figure Legend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p
    Figure Legend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Techniques Used: Expressing, Ligation

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  • 85
    Alomone Labs anti bestrophin 1 extracellular antibody
    <t>Bestrophin-1</t> is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 <t>(Best-1)</t> at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or <t>antibodies,</t> respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.
    Anti Bestrophin 1 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bestrophin 1 extracellular antibody/product/Alomone Labs
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    anti bestrophin 1 extracellular antibody - by Bioz Stars, 2022-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Journal: Molecular Pain

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    doi: 10.1186/s12990-015-0042-1

    Figure Lengend Snippet: Bestrophin-1 is expressed in the spinal cord and DRG. RT-PCR ( a , b ) and western blot ( c , d ) analysis of bestrophin-1 (Best-1) at the ipsilateral dorsal portion of the spinal cord (SC) and DRG obtained from naïve (Na), sham (S) and spinal nerve ligated (SNL) rats. Insets show a representative band of the PCR product ( upper panels ) and immunoblot ( lower panels ) obtained with the specific bestrophin-1 and β-actin primers or antibodies, respectively. Data were normalized against β-actin and are expressed as the mean ± SEM of three independent rats. Note that spinal nerve injury did not modify Best-1 mRNA and protein expression.

    Article Snippet: Membranes were blocked with 5% non-fat milk in phosphate-buffered saline at pH 7.4 containing (in mM) (137 NaCl, 2.7 KCl, 10 Na2 HPO4 and 2 KH2 PO4) with Tween 0.05% and they were incubated at 4°C overnight with rabbit anti-bestrophin-1 (ABC-001, 1:200; Alomone Labs, Jerusalem, Israel) or rabbit anti-anoctamin-1 (ACL-011, 1:100; Alomone Labs, Jerusalem, Israel).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction, Expressing

    Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Journal: Molecular Pain

    Article Title: Role of anoctamin-1 and bestrophin-1 in spinal nerve ligation-induced neuropathic pain in rats

    doi: 10.1186/s12990-015-0042-1

    Figure Lengend Snippet: Intrathecal administration of selective antibody anti-bestrophin-1 or anti-anoctamin-1 reverses established allodynia and reduces protein expression. Time-course of the antiallodynic effect of antibodies against bestrophin-1 (Best-1 ab, 2 μg/6 h × 30 h; a ) or anoctamin-1 (Ano-1 ab, 2 μg/6 h x 30 h; d ) in rats subjected to L5/L6 spinal nerve ligation. Paw withdrawal threshold was assessed every 6 h starting 12 days after spinal nerve ligation. Data are presented as the mean ± SEM for six animals. *Significantly different from the vehicle group (p

    Article Snippet: Membranes were blocked with 5% non-fat milk in phosphate-buffered saline at pH 7.4 containing (in mM) (137 NaCl, 2.7 KCl, 10 Na2 HPO4 and 2 KH2 PO4) with Tween 0.05% and they were incubated at 4°C overnight with rabbit anti-bestrophin-1 (ABC-001, 1:200; Alomone Labs, Jerusalem, Israel) or rabbit anti-anoctamin-1 (ACL-011, 1:100; Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Ligation

    (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass

    Journal: British Journal of Pharmacology

    Article Title: Chloride channels mediate sodium sulphide-induced relaxation in rat uteri

    doi: 10.1111/bph.13161

    Figure Lengend Snippet: (A) RT-PCR data showing quantitative evidence of BEST-1 in rat uterus in oestrus compared with endogenous controls (GAPDH, actin, cyclin A and 18S). (B) Representative Western blot demonstrating bands (four different animals) of expected molecular mass

    Article Snippet: Membranes were blocked for 1 h with 2% non-fat dry milk dissolved in PBS at room temperature and incubated overnight at 4°C with anti-BEST-1 primary antibody (CAT, 1:2000, Alomone Laboratories, Jerusalem, Israel).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot