antibodies against nav β1  (Alomone Labs)


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    Alomone Labs antibodies against nav β1
    Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking <t>β1</t> subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P
    Antibodies Against Nav β1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against nav β1/product/Alomone Labs
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    antibodies against nav β1 - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons"

    Article Title: Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.01510

    Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P
    Figure Legend Snippet: Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P

    Techniques Used: Blocking Assay, Co-Immunoprecipitation Assay, Binding Assay

    2) Product Images from "Tanshinone II-A sodium sulfonate (DS-201) enhances human BKCa channel activity by selectively targeting the pore-forming α subunit"

    Article Title: Tanshinone II-A sodium sulfonate (DS-201) enhances human BKCa channel activity by selectively targeting the pore-forming α subunit

    Journal: Acta Pharmacologica Sinica

    doi: 10.1038/aps.2014.85

    DS-201 upregulated the expression of the BK Ca channel α subunit but did not affect the expression of the β1 subunit. (A and B) Western blotting showing the effects of 80 μmol/L DS-201 on the total and membranous protein expression levels of the α and β1 subunits in HEK293 cells. Endogenous β-actin and Na + /K + -ATPase (NKA) served as loading controls, respectively. (C) Statistical results showing the enhancing effects of 80 μmol/L DS-201 on the membranous and total expressions of the α subunit but not of the β1 subunit in HEK293 cells. The signal intensity of each band was normalized to the band of β-actin or NKA. The effects of DS-201 were expressed as fold changes compared with control. (D) Effects of 80 μmol/L DS-201 on the total protein expression levels of the α and β subunits in cultured VSMCs. (E) Confocal images showing that DS-201 enhanced the expression of the BK Ca α subunit (upper, green) but did not affect the expression of the β1 subunit (lower, red) in cultured VSMCs. Scale bar=50 μm. (F) Co-IP assay showing the effects of DS-201 on the interaction between the α (Flag-tagged) and β1 (His-tagged) subunits in HEK293 cells. The statistical results (lower) showed that DS-201 did not affect the ratio of hβ1 to hSlo or of hSlo to hβ1, when either anti-Flag or anti-His was used as the bait. Mean±SEM. a P > 0.05, b P
    Figure Legend Snippet: DS-201 upregulated the expression of the BK Ca channel α subunit but did not affect the expression of the β1 subunit. (A and B) Western blotting showing the effects of 80 μmol/L DS-201 on the total and membranous protein expression levels of the α and β1 subunits in HEK293 cells. Endogenous β-actin and Na + /K + -ATPase (NKA) served as loading controls, respectively. (C) Statistical results showing the enhancing effects of 80 μmol/L DS-201 on the membranous and total expressions of the α subunit but not of the β1 subunit in HEK293 cells. The signal intensity of each band was normalized to the band of β-actin or NKA. The effects of DS-201 were expressed as fold changes compared with control. (D) Effects of 80 μmol/L DS-201 on the total protein expression levels of the α and β subunits in cultured VSMCs. (E) Confocal images showing that DS-201 enhanced the expression of the BK Ca α subunit (upper, green) but did not affect the expression of the β1 subunit (lower, red) in cultured VSMCs. Scale bar=50 μm. (F) Co-IP assay showing the effects of DS-201 on the interaction between the α (Flag-tagged) and β1 (His-tagged) subunits in HEK293 cells. The statistical results (lower) showed that DS-201 did not affect the ratio of hβ1 to hSlo or of hSlo to hβ1, when either anti-Flag or anti-His was used as the bait. Mean±SEM. a P > 0.05, b P

    Techniques Used: Expressing, Western Blot, Cell Culture, Co-Immunoprecipitation Assay

    3) Product Images from "Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field"

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    Journal: Frontiers in Neuroanatomy

    doi: 10.3389/fnana.2020.574130

    Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.
    Figure Legend Snippet: Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.

    Techniques Used: Expressing, Staining, Labeling

    Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.
    Figure Legend Snippet: Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.

    Techniques Used: Expressing

    Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p
    Figure Legend Snippet: Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p

    Techniques Used: Expressing, Marker, Labeling

    Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p
    Figure Legend Snippet: Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p

    Techniques Used: Expressing, Labeling

    Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.
    Figure Legend Snippet: Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.

    Techniques Used: Expressing

    4) Product Images from "Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons"

    Article Title: Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.01510

    Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P
    Figure Legend Snippet: Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P

    Techniques Used: Blocking Assay, Co-Immunoprecipitation Assay, Binding Assay

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  • 93
    Alomone Labs antibodies against nav β1
    Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking <t>β1</t> subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P
    Antibodies Against Nav β1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against nav β1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against nav β1 - by Bioz Stars, 2022-08
    93/100 stars
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    Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons

    doi: 10.3389/fphar.2018.01510

    Figure Lengend Snippet: Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P

    Article Snippet: The cryostat sections (12 μm) were blocked with 3% donkey serum in 0.3% Triton X-100 for 1 h at room temperature and incubated in primary antibodies against Nav β1 (1:200, rabbit, Alomone Labs, Israel), Nav 1.8 (1:200, mouse, Abcam, United Kingdom) at 4°C overnight.

    Techniques: Blocking Assay, Co-Immunoprecipitation Assay, Binding Assay

    DS-201 upregulated the expression of the BK Ca channel α subunit but did not affect the expression of the β1 subunit. (A and B) Western blotting showing the effects of 80 μmol/L DS-201 on the total and membranous protein expression levels of the α and β1 subunits in HEK293 cells. Endogenous β-actin and Na + /K + -ATPase (NKA) served as loading controls, respectively. (C) Statistical results showing the enhancing effects of 80 μmol/L DS-201 on the membranous and total expressions of the α subunit but not of the β1 subunit in HEK293 cells. The signal intensity of each band was normalized to the band of β-actin or NKA. The effects of DS-201 were expressed as fold changes compared with control. (D) Effects of 80 μmol/L DS-201 on the total protein expression levels of the α and β subunits in cultured VSMCs. (E) Confocal images showing that DS-201 enhanced the expression of the BK Ca α subunit (upper, green) but did not affect the expression of the β1 subunit (lower, red) in cultured VSMCs. Scale bar=50 μm. (F) Co-IP assay showing the effects of DS-201 on the interaction between the α (Flag-tagged) and β1 (His-tagged) subunits in HEK293 cells. The statistical results (lower) showed that DS-201 did not affect the ratio of hβ1 to hSlo or of hSlo to hβ1, when either anti-Flag or anti-His was used as the bait. Mean±SEM. a P > 0.05, b P

    Journal: Acta Pharmacologica Sinica

    Article Title: Tanshinone II-A sodium sulfonate (DS-201) enhances human BKCa channel activity by selectively targeting the pore-forming α subunit

    doi: 10.1038/aps.2014.85

    Figure Lengend Snippet: DS-201 upregulated the expression of the BK Ca channel α subunit but did not affect the expression of the β1 subunit. (A and B) Western blotting showing the effects of 80 μmol/L DS-201 on the total and membranous protein expression levels of the α and β1 subunits in HEK293 cells. Endogenous β-actin and Na + /K + -ATPase (NKA) served as loading controls, respectively. (C) Statistical results showing the enhancing effects of 80 μmol/L DS-201 on the membranous and total expressions of the α subunit but not of the β1 subunit in HEK293 cells. The signal intensity of each band was normalized to the band of β-actin or NKA. The effects of DS-201 were expressed as fold changes compared with control. (D) Effects of 80 μmol/L DS-201 on the total protein expression levels of the α and β subunits in cultured VSMCs. (E) Confocal images showing that DS-201 enhanced the expression of the BK Ca α subunit (upper, green) but did not affect the expression of the β1 subunit (lower, red) in cultured VSMCs. Scale bar=50 μm. (F) Co-IP assay showing the effects of DS-201 on the interaction between the α (Flag-tagged) and β1 (His-tagged) subunits in HEK293 cells. The statistical results (lower) showed that DS-201 did not affect the ratio of hβ1 to hSlo or of hSlo to hβ1, when either anti-Flag or anti-His was used as the bait. Mean±SEM. a P > 0.05, b P

    Article Snippet: Samples containing approximately 50 μg of total protein were separated with SDS-PAGE and were transferred to PVDF membrane, followed by blocking and incubation with anti-Slo (1:500, Alomone, Jerusalem, Israel) or anti-β1 (1:500, Alomone, Jerusalem, Israel) primary antibodies at 4 °C overnight.

    Techniques: Expressing, Western Blot, Cell Culture, Co-Immunoprecipitation Assay

    Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing, Staining, Labeling

    Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing

    Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing, Marker, Labeling

    Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing, Labeling

    Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing

    Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P

    Journal: Frontiers in Pharmacology

    Article Title: Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons

    doi: 10.3389/fphar.2018.01510

    Figure Lengend Snippet: Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P

    Article Snippet: The cryostat sections (12 μm) were blocked with 3% donkey serum in 0.3% Triton X-100 for 1 h at room temperature and incubated in primary antibodies against Nav β1 (1:200, rabbit, Alomone Labs, Israel), Nav1.8 (1:200, mouse, Abcam, United Kingdom) at 4°C overnight.

    Techniques: Blocking Assay, Co-Immunoprecipitation Assay, Binding Assay