α2b  (Alomone Labs)


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    Alomone Labs α2b
    α2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α2b/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    α2b - by Bioz Stars, 2022-12
    93/100 stars

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    • antibodies against α2a  (Alomone Labs)
    94
    Alomone Labs antibodies against α2a
    The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not <t>α2A</t> receptor proteins in the mouse left ventricular tissue.
    Antibodies Against α2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against α2a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against α2a - by Bioz Stars, 2022-12
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    anti adra2b  (Alomone Labs)
    93
    Alomone Labs anti adra2b
    EKLF-dependent signature genes in F4/80+ fetal liver (FL) macrophages. ( A ) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Figure 1E ) that are also enriched in F4/80+ EKLF/GFP+ cells (source data: Figure 5—figure supplement 2—source data 1 ). ( B ) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/- (source data: Figure 5—figure supplement 2—source data 2 ). Red boxes depict the EKLF-dependent signature genes that are common to both ( A ) and ( B ). ( C ) Density plot of flow cytometry analysis of E13.5 FLs stained with F4/80 and <t>Adra2b</t> antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double-positive Adra2b+ and F4/80-hi cells, compared to total Adra2b+ cells, is indicated. ( D ) Representative pictures of erythroblastic islands from E13.5 FLs are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue). Expression values of signature genes from Figure 1E that are significantly enriched in EKLF/GFP+ cells. Expression of signature genes from Figure 1E that are significantly downregulated in EKLF-/- cells compared to WT.
    Anti Adra2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti alpha2b adrenergic receptor extracellular antibody  (Alomone Labs)
    92
    Alomone Labs anti alpha2b adrenergic receptor extracellular antibody
    EKLF-dependent signature genes in F4/80+ fetal liver (FL) macrophages. ( A ) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Figure 1E ) that are also enriched in F4/80+ EKLF/GFP+ cells (source data: Figure 5—figure supplement 2—source data 1 ). ( B ) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/- (source data: Figure 5—figure supplement 2—source data 2 ). Red boxes depict the EKLF-dependent signature genes that are common to both ( A ) and ( B ). ( C ) Density plot of flow cytometry analysis of E13.5 FLs stained with F4/80 and <t>Adra2b</t> antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double-positive Adra2b+ and F4/80-hi cells, compared to total Adra2b+ cells, is indicated. ( D ) Representative pictures of erythroblastic islands from E13.5 FLs are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue). Expression values of signature genes from Figure 1E that are significantly enriched in EKLF/GFP+ cells. Expression of signature genes from Figure 1E that are significantly downregulated in EKLF-/- cells compared to WT.
    Anti Alpha2b Adrenergic Receptor Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti alpha2b adrenergic receptor extracellular antibody - by Bioz Stars, 2022-12
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    The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

    doi: 10.3390/ijms22084135

    Figure Lengend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Article Snippet: Western BlotProteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

    doi: 10.3390/ijms22084135

    Figure Lengend Snippet: BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

    Article Snippet: Western BlotProteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

    Techniques: Inhibition

    EKLF-dependent signature genes in F4/80+ fetal liver (FL) macrophages. ( A ) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Figure 1E ) that are also enriched in F4/80+ EKLF/GFP+ cells (source data: Figure 5—figure supplement 2—source data 1 ). ( B ) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/- (source data: Figure 5—figure supplement 2—source data 2 ). Red boxes depict the EKLF-dependent signature genes that are common to both ( A ) and ( B ). ( C ) Density plot of flow cytometry analysis of E13.5 FLs stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double-positive Adra2b+ and F4/80-hi cells, compared to total Adra2b+ cells, is indicated. ( D ) Representative pictures of erythroblastic islands from E13.5 FLs are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue). Expression values of signature genes from Figure 1E that are significantly enriched in EKLF/GFP+ cells. Expression of signature genes from Figure 1E that are significantly downregulated in EKLF-/- cells compared to WT.

    Journal: eLife

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    doi: 10.7554/eLife.61070

    Figure Lengend Snippet: EKLF-dependent signature genes in F4/80+ fetal liver (FL) macrophages. ( A ) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Figure 1E ) that are also enriched in F4/80+ EKLF/GFP+ cells (source data: Figure 5—figure supplement 2—source data 1 ). ( B ) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/- (source data: Figure 5—figure supplement 2—source data 2 ). Red boxes depict the EKLF-dependent signature genes that are common to both ( A ) and ( B ). ( C ) Density plot of flow cytometry analysis of E13.5 FLs stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double-positive Adra2b+ and F4/80-hi cells, compared to total Adra2b+ cells, is indicated. ( D ) Representative pictures of erythroblastic islands from E13.5 FLs are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue). Expression values of signature genes from Figure 1E that are significantly enriched in EKLF/GFP+ cells. Expression of signature genes from Figure 1E that are significantly downregulated in EKLF-/- cells compared to WT.

    Article Snippet: Flow cytometrySuspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

    Techniques: Flow Cytometry, Staining, Immunostaining, Expressing

    EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

    Journal: eLife

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    doi: 10.7554/eLife.61070

    Figure Lengend Snippet: EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

    Article Snippet: Flow cytometrySuspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

    Techniques: Expressing

    EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and Feature plots (right) showing individual cellular mRNA expression of (A) Klf1, (B) Epor, and (C) Adra2b superimposed on the clusters.

    Journal: bioRxiv

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    doi: 10.1101/2020.12.23.424143

    Figure Lengend Snippet: EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and Feature plots (right) showing individual cellular mRNA expression of (A) Klf1, (B) Epor, and (C) Adra2b superimposed on the clusters.

    Article Snippet: Flow CytometrySuspended cells from fetal livers were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-Adducinβ (Santa Cruz # sc-376063), and anti-Spectrinβ1 (Santa Cruz # sc-374309).

    Techniques: Expressing

    EKLF dependent signature genes in F4/80+ FL macrophages. (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Fig. 1E ) that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).

    Journal: bioRxiv

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    doi: 10.1101/2020.12.23.424143

    Figure Lengend Snippet: EKLF dependent signature genes in F4/80+ FL macrophages. (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Fig. 1E ) that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).

    Article Snippet: Flow CytometrySuspended cells from fetal livers were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-Adducinβ (Santa Cruz # sc-376063), and anti-Spectrinβ1 (Santa Cruz # sc-374309).

    Techniques: Flow Cytometry, Staining, Immunostaining