α2b (Alomone Labs)

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Alomone Labs α2b
α2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/α2b/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

α2b - by Bioz Stars, 2022-05

93/100 stars

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anti adra2b (Alomone Labs)

Alomone Labs anti adra2b

EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and Feature plots (right) showing individual cellular mRNA expression of (A) Klf1, (B) Epor, and (C) <t>Adra2b</t> superimposed on the clusters.

Anti Adra2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti adra2b/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti adra2b - by Bioz Stars, 2022-05

93/100 stars

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antibodies against α2a (Alomone Labs)

Alomone Labs antibodies against α2a

The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not <t>α2A</t> receptor proteins in the mouse left ventricular tissue.

Antibodies Against α2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against α2a/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99

antibodies against α2a - by Bioz Stars, 2022-05

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Journal: bioRxiv

Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

doi: 10.1101/2020.12.23.424143

Figure Lengend Snippet: EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and Feature plots (right) showing individual cellular mRNA expression of (A) Klf1, (B) Epor, and (C) Adra2b superimposed on the clusters.

Article Snippet: Flow CytometrySuspended cells from fetal livers were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-Adducinβ (Santa Cruz # sc-376063), and anti-Spectrinβ1 (Santa Cruz # sc-374309).

Techniques: Expressing

EKLF dependent signature genes in F4/80+ FL macrophages. (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Fig. 1E ) that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).

Journal: bioRxiv

Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

doi: 10.1101/2020.12.23.424143

Figure Lengend Snippet: EKLF dependent signature genes in F4/80+ FL macrophages. (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Fig. 1E ) that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).

Techniques: Flow Cytometry, Staining, Immunostaining

EKLF-dependent signature genes in F4/80+ fetal liver (FL) macrophages. ( A ) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Figure 1E ) that are also enriched in F4/80+ EKLF/GFP+ cells (source data: Figure 5—figure supplement 2—source data 1 ). ( B ) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/- (source data: Figure 5—figure supplement 2—source data 2 ). Red boxes depict the EKLF-dependent signature genes that are common to both ( A ) and ( B ). ( C ) Density plot of flow cytometry analysis of E13.5 FLs stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double-positive Adra2b+ and F4/80-hi cells, compared to total Adra2b+ cells, is indicated. ( D ) Representative pictures of erythroblastic islands from E13.5 FLs are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue). Expression values of signature genes from Figure 1E that are significantly enriched in EKLF/GFP+ cells. Expression of signature genes from Figure 1E that are significantly downregulated in EKLF-/- cells compared to WT.

Journal: eLife

Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

doi: 10.7554/eLife.61070

Figure Lengend Snippet: EKLF-dependent signature genes in F4/80+ fetal liver (FL) macrophages. ( A ) Heatmap of log2 FPKM values of F4/80+ FL signature genes ( Figure 1E ) that are also enriched in F4/80+ EKLF/GFP+ cells (source data: Figure 5—figure supplement 2—source data 1 ). ( B ) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/- (source data: Figure 5—figure supplement 2—source data 2 ). Red boxes depict the EKLF-dependent signature genes that are common to both ( A ) and ( B ). ( C ) Density plot of flow cytometry analysis of E13.5 FLs stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double-positive Adra2b+ and F4/80-hi cells, compared to total Adra2b+ cells, is indicated. ( D ) Representative pictures of erythroblastic islands from E13.5 FLs are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue). Expression values of signature genes from Figure 1E that are significantly enriched in EKLF/GFP+ cells. Expression of signature genes from Figure 1E that are significantly downregulated in EKLF-/- cells compared to WT.

Article Snippet: Flow cytometrySuspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

Techniques: Flow Cytometry, Staining, Immunostaining, Expressing

Journal: eLife

Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

doi: 10.7554/eLife.61070

Figure Lengend Snippet: EKLF/Klf1-expressing clusters in F4/80+ fetal liver macrophages. Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

doi: 10.3390/ijms22084135

Figure Lengend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

Article Snippet: Western BlotProteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

Journal: International Journal of Molecular Sciences

Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

doi: 10.3390/ijms22084135

Figure Lengend Snippet: BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

Techniques: Inhibition