anti α2a adrenergic receptor  (Alomone Labs)


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    Alomone Labs anti α2a adrenergic receptor
    Expression of adrenergic receptors in FEF. From left to right: expression of α1A, <t>α2A,</t> β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.
    Anti α2a Adrenergic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti α2a adrenergic receptor/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti α2a adrenergic receptor - by Bioz Stars, 2022-05
    94/100 stars

    Images

    1) Product Images from "Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field"

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    Journal: Frontiers in Neuroanatomy

    doi: 10.3389/fnana.2020.574130

    Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.
    Figure Legend Snippet: Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.

    Techniques Used: Expressing, Staining, Labeling

    Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.
    Figure Legend Snippet: Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.

    Techniques Used: Expressing

    Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p
    Figure Legend Snippet: Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p

    Techniques Used: Expressing, Marker, Labeling

    Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p
    Figure Legend Snippet: Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p

    Techniques Used: Expressing, Labeling

    Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.
    Figure Legend Snippet: Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.

    Techniques Used: Expressing

    2) Product Images from "Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes"

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22084135

    The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.
    Figure Legend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.
    Figure Legend Snippet: BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

    Techniques Used: Inhibition

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    Alomone Labs anti α2a adrenergic receptor
    Expression of adrenergic receptors in FEF. From left to right: expression of α1A, <t>α2A,</t> β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.
    Anti α2a Adrenergic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti α2a adrenergic receptor/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti α2a adrenergic receptor - by Bioz Stars, 2022-05
    94/100 stars
      Buy from Supplier

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    Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors in FEF. From left to right: expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) in macaque FEF. Images show a cross-section of all layers of cortex and are oriented with the pial surface at the top and white matter at the bottom. The α2A and β2 adrenergic receptors had strong, punctate staining of cell bodies, with little to no background labeling of processes. While the α1A and β1 adrenergic receptors also had strong, punctate staining of cell bodies, there was also staining of the surrounding processes (dendrites and axons), which resulted in a higher amount of background signal. Scale bar = 100 μm for all panels.

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing, Staining, Labeling

    Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors across cell types and layers. (A) The number of different classes of cell types per mm 2 across FEF layers. General classes of pyramidal neurons (neurogranin and RP) are more abundant than any other class of neuron across layers II through VI. There is low expression of all receptor classes in layer I where there are few neurons. (B) For each of the four adrenergic receptors (pale to dark: α1A, α2A, β1, β2) we quantified the proportion of each cell type that expressed that receptor across all cortical layers in the FEF. We found that expression was very consistent for any given receptor/cell type pair.

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing

    Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors on pyramidal neurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with pyramidal neuron markers (RP, neurogranin, and SMI-32) from left to right. RP and neurogranin are both putative general markers of pyramidal neurons and SMI-32 is a marker for putative long-range projecting pyramidal neurons. All adrenergic receptors are labeled in green, and all pyramidal neurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. All four adrenergic receptors were expressed significantly more highly on long-range projecting pyramidal neurons than either class of general pyramidal neuron. Significance levels are noted as *** p

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing, Marker, Labeling

    Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Expression of adrenergic receptors on inhibitory interneurons. (A) Panels show expression of α1A, α2A, β1, and β2 adrenergic receptors (α1AR, α2AR, β1R, and β2R, respectively) from top to bottom with inhibitory interneuron markers (parvalbumin, calbindin and calretinin) from left to right. All adrenergic receptors are labeled in green, and all inhibitory interneurons are labeled in magenta. (B) Quantification of the proportion of each neuron class that expressed each receptor class. Chi-squared tests were performed using pooled neuron counts across all animals. Lines above the bars show the significance of different comparisons. Black lines indicate significant differences between the expression of different receptors within a neuron class; gray lines indicate significant differences of expression of a specific receptor across different neuron classes. The shade of gray indicates which receptor class is being compared and matches the shading of the bars: from light to dark—α1AR, α2AR, β1R, and β2R. Significance levels are noted as *** p

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing, Labeling

    Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.

    Journal: Frontiers in Neuroanatomy

    Article Title: Differences in Noradrenaline Receptor Expression Across Different Neuronal Subtypes in Macaque Frontal Eye Field

    doi: 10.3389/fnana.2020.574130

    Figure Lengend Snippet: Density of adrenergic receptors across different layers of the FEF. The number of neurons per mm 2 that express a given receptor across FEF layers. α2A adrenergic receptors (α2ARs) and β2 adrenergic receptors (β2Rs) are more abundant than either α1A adrenergic receptors (α1ARs) or β1 adrenergic receptors (β1Rs) across layers II through V. There are no obvious differences in expression across layers other than the predictably low expression of all receptor classes in layer I where there are few neurons.

    Article Snippet: Membranes were blocked with Intercept Blocking Buffer (LiCor 927-70001) and then incubated overnight with anti-α1A adrenergic receptor (Alomone Labs AAR-015), anti-α2A adrenergic receptor (Alomone Labs AAR-020), anti-β1 adrenergic receptor (Alomone Labs AAR-023), or anti-β2 adrenergic receptor (Alomone Labs AAR-016) pre-incubated with peptide plus 1% BSA, or pre-incubated with 1% BSA alone.

    Techniques: Expressing

    The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

    doi: 10.3390/ijms22084135

    Figure Lengend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Article Snippet: Western BlotProteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca2+ Current in Mouse Cardiac Myocytes

    doi: 10.3390/ijms22084135

    Figure Lengend Snippet: BRL 44408, a specific antagonist of the α2A isoform, did not affect the guanabenz-induced suppression of I CaL . ( a , b ) Representative I CaL recordings and time-course of peak I CaL density values in the presence of guanabenz (guan) and BRL 44408. Horizontal bars denote the protocol of the drug applications. ( c ) BRL 44408 did not induce a significant rightward shift of dose-dependent inhibition of I CaL by guanabenz. Curves represent the Hill’s fits with parameters indicated in the text.

    Article Snippet: Western BlotProteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

    Techniques: Inhibition