Anti Adra1b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor"
Article Title: L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor
Journal: JCI Insight
Figure Legend Snippet: Interaction between GPR143 and ADRA1B. ( A ) Co-immunoprecipitation (Co-IP) of ADRA1B-Myc and GPR143-HA. Bands were detected by anti-Myc (left) or by anti-HA (right) antibodies. ( B ) Protein levels of pERK and whole ERK 10 minutes after the treatment with phenylephrine. ( C ) Summarized data of pERK/ERK (% of control) in HEK293 cells coexpressing ADRA1B-Myc and free-EGFP or ADRA1B-Myc and GPR143-EGFP ( F 1,32 = 7.899, P
Techniques Used: Immunoprecipitation, Co-Immunoprecipitation Assay
Figure Legend Snippet: Immunohistochemical staining of endogenous GPR143 and ADRA1B. Expression of GPR143 ( A ) and ADRA1B ( C ) in the liver (arrowheads). No GPR143 signals were observed in the liver of Gpr143 –/y mice ( B ). No ADRA1B signals were observed in sections incubated with the antigenic peptide–preadsorbed antibody ( D ). Scale bar: 100 μm. Endogenous interaction between GPR143 and ADRA1B. ( E ) The GPR143-ADRA1B interacting signals (green) were detected by in situ proximity ligation assay technology in WT liver (arrowheads). ( F ) No interacting signals were observed in Gpr143 –/y liver ( n = 2, independent experiments). The nucleus was counterstained by DAPI (blue). Scale bars: 5 μm.
Techniques Used: Immunohistochemistry, Staining, Expressing, Mouse Assay, Incubation, In Situ, Proximity Ligation Assay