at2 (Alomone Labs)


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At2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/at2/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)"
Article Title: Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)
Journal: Cellular signalling
doi: 10.1016/j.cellsig.2010.07.012

Figure Legend Snippet: Regulation of VEGF, fibronectin and laminin β1 expression in kidneys from hyperglycemic AT2KO mice A. AT2 protein level was measured by immunoblot using a specific antibody. The lower panel shows combined data obtained on kidneys from 5 individual mice for each experimental condition. B. VEGF protein (B) and mRNA (C) levels were measured by immunoblot and RT-qPCR, respectively, on the same number of kidneys as in panel A. Identical experiments were performed for the protein and mRNA levels of fibronectin (D - E) and laminin β1 (F - G). **p
Techniques Used: Expressing, Mouse Assay, Quantitative RT-PCR

Figure Legend Snippet: VEGF mRNA translation in hyperglycemic kidneys A. VEGF mRNA translation was studied by polysome assay. The shift of VEGF mRNA toward the heaviest fractions in diabetic kidneys indicates active translation, and is reversed by the AT2 but not the AT1 antagonist. Note that the distribution of GAPDH mRNA is unaffected by the various treatments. The figure is representative of experiments performed on kidneys from 3 individual mice. B. Association of hnRNP K with VEGF mRNA was studied by immunoprecipitation with anti-hnRNP K antibody followed by extraction of associated RNA associated and RT-PCR. Note that hnRNP K is constitutively associated with both VEGF and GAPDH mRNAs and that only the association with VEGF mRNA is increased in diabetic kidneys. The figure is representative of experiments performed on kidneys from 3 individual mice. **p
Techniques Used: Mouse Assay, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction

Figure Legend Snippet: Angiotensin receptors are expressed in normal murine kidneys AT1 (A) and AT2 (B) were detected in kidney slices as described in the methods section. Arrows indicate localization of AT1 and AT2. Magnification = 20X. AT1 is strongly expressed in the apical and lateral membranes of the tubules (thin arrows), and in mesangial cells (thick arrow),.AT2 is expressed mostly in glomerular endothelial cells (thick arrow) and in the proximal tubules (thin arrow). The pictures are representative of kidneys from 2 individual mice. C) detection of AT1 and AT2 by western blot in kidney cortex homogenates using the same antibodies as in A and B.
Techniques Used: Mouse Assay, Western Blot

Figure Legend Snippet: Signaling pathways activated by AT1 and AT2 in diabetic kidneys The diagram depicts a summary of the data obtained in this study. AT1 activation by hyperglycemia activates mTOR independently of Akt and leads to inactivation of eEF2K but is not sufficient to cause the dephosphorylation and activation of eEF2. Activation of AT2 stimulates the Akt-mTOR pathway that inactivates eEF2K and activates PP2A that dephosphorylates eEF2; AT2 also mediates hyperglycemia-induced increment in eEF1A. Activation of eEF2 and up-regulation of eEF1A stimulate elongation phase of mRNA translation, leading to increased translation of VEGF mRNA.
Techniques Used: Activation Assay, De-Phosphorylation Assay
2) Product Images from "Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)"
Article Title: Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)
Journal: Cellular signalling
doi: 10.1016/j.cellsig.2010.07.012

Figure Legend Snippet: Regulation of VEGF, fibronectin and laminin β1 expression in kidneys from hyperglycemic AT2KO mice A. AT2 protein level was measured by immunoblot using a specific antibody. The lower panel shows combined data obtained on kidneys from 5 individual mice for each experimental condition. B. VEGF protein (B) and mRNA (C) levels were measured by immunoblot and RT-qPCR, respectively, on the same number of kidneys as in panel A. Identical experiments were performed for the protein and mRNA levels of fibronectin (D - E) and laminin β1 (F - G). **p
Techniques Used: Expressing, Mouse Assay, Quantitative RT-PCR

Figure Legend Snippet: VEGF mRNA translation in hyperglycemic kidneys A. VEGF mRNA translation was studied by polysome assay. The shift of VEGF mRNA toward the heaviest fractions in diabetic kidneys indicates active translation, and is reversed by the AT2 but not the AT1 antagonist. Note that the distribution of GAPDH mRNA is unaffected by the various treatments. The figure is representative of experiments performed on kidneys from 3 individual mice. B. Association of hnRNP K with VEGF mRNA was studied by immunoprecipitation with anti-hnRNP K antibody followed by extraction of associated RNA associated and RT-PCR. Note that hnRNP K is constitutively associated with both VEGF and GAPDH mRNAs and that only the association with VEGF mRNA is increased in diabetic kidneys. The figure is representative of experiments performed on kidneys from 3 individual mice. **p
Techniques Used: Mouse Assay, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction

Figure Legend Snippet: Angiotensin receptors are expressed in normal murine kidneys AT1 (A) and AT2 (B) were detected in kidney slices as described in the methods section. Arrows indicate localization of AT1 and AT2. Magnification = 20X. AT1 is strongly expressed in the apical and lateral membranes of the tubules (thin arrows), and in mesangial cells (thick arrow),.AT2 is expressed mostly in glomerular endothelial cells (thick arrow) and in the proximal tubules (thin arrow). The pictures are representative of kidneys from 2 individual mice. C) detection of AT1 and AT2 by western blot in kidney cortex homogenates using the same antibodies as in A and B.
Techniques Used: Mouse Assay, Western Blot

Figure Legend Snippet: Signaling pathways activated by AT1 and AT2 in diabetic kidneys The diagram depicts a summary of the data obtained in this study. AT1 activation by hyperglycemia activates mTOR independently of Akt and leads to inactivation of eEF2K but is not sufficient to cause the dephosphorylation and activation of eEF2. Activation of AT2 stimulates the Akt-mTOR pathway that inactivates eEF2K and activates PP2A that dephosphorylates eEF2; AT2 also mediates hyperglycemia-induced increment in eEF1A. Activation of eEF2 and up-regulation of eEF1A stimulate elongation phase of mRNA translation, leading to increased translation of VEGF mRNA.
Techniques Used: Activation Assay, De-Phosphorylation Assay