at1r  (Alomone Labs)

Journal: Asian Journal of Andrology

Article Title: Local renin angiotensin system and sperm DNA fragmentation

doi: 10.4103/aja202150

Figure Lengend Snippet: Schematic representation of the sperm renin angiotensin system, with the alterations (in bold) that occur in its axes in spermatozoa with DNA fragmentation. Due to the principal location of the enzymes and receptors altered in this principal axis of renin angiotensin system (RAS) (the tail of spermatozoa), it could be suggested that the spermatic motility is modified in DNA fragmented cells. ACE: angiotensin-converting enzyme; NEP: neutral endopeptidase; PEP: prolyl endopeptidase; MasR: angiotensin (A) 1–7 or Mas receptor; APN: aminopeptidase N; APA: aminopeptidase A; APB: aminopeptidase B; AT1R: A II receptor type 1; AT2R: A II receptor type 2; AT4R: A IV receptor; PRR: (pro)renin receptor.

Article Snippet: The samples were incubated in blocking buffer (PBS with 10% [w /v] fetal bovine serum [FBS]; Biochrom, Cambridge, UK) for 30 min and then with the following primary antibodies: anti-AT1R (extracellular) antibody (rabbit polyclonal antibody to AT1R; AAR011; Alomone Labs, Jerusalem, Israel), anti-AT2R antibody (rabbit polyclonal; ab19134; Abcam, Cambridge, UK), anti-AT4R antibody (Insulin-regulated aminopeptidase [IRAP]; rabbit polyclonal; clone H-133, sc-135229; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-renin receptor (rabbit polyclonal; clone H-85, sc-67390; Santa Cruz Biotechnology), anti-MasR (rabbit polyclonal; AAR-013; Alomone Labs), PE-labeled human ACE monoclonal phycoerythrin (PE mouse anti-human CD143; 344204; BioLegend, San Diego, CA, USA), PE anti-human CD13 (PE mouse anti-human CD13; 555394; BD Biosciences), and PE mouse anti-human CD10 (PE mouse anti-human CD10; 555375; BD Biosciences).

Techniques: Modification

Journal: Therapeutic Advances in Endocrinology and Metabolism

Article Title: The role of the renin–angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data

doi: 10.1177/2042018820920560

Figure Lengend Snippet: Expression of AT1-R and AT2-R in the peri-implantation endometrium. Representative micrographs of immunohistochemical stain of (a) AT1-R in fertile control, (b) AT1-R in RM, (c) AT2-R in fertile control, (d) AT2-R in RM. Magnification ×200. Scale bar = 200 μm. AT1-R, angiotensin type 1 receptor; AT2-R, angiotensin type 2 receptor; BV, blood vessels; GE, glandular epithelium; LE, luminal epithelium; RM, recurrent miscarriage; ST, stroma.

Article Snippet: The primary antibodies were rabbit anti-human angiotensin II receptor type-1 polyclonal antibody (AAR-011, Alomone Labs, Jerusalem, Israel) at a dilution of 1:100 and rabbit anti-human angiotensin II type 2 receptor antibody (ab19134, AbCam, UK) at a dilution of 1:100.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Therapeutic Advances in Endocrinology and Metabolism

Article Title: The role of the renin–angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data

doi: 10.1177/2042018820920560

Figure Lengend Snippet: The possible pathways of Ang II-mediated angiogenesis. ANG II stimulates the generation of ROS through membrane NAD(P)H oxidases in VSMCs after binding to AT1-R. ROS are involved in many Ang II mediated effects, including production of HIF-1α in vascular cells, activation of p38MAPK, and transcription factor NF-kB. Interactions between Ang II and ROS are critical in vascular physiology and pathology in terms of regulating vascular structure and functions. Ang-Tie could also trigger the production of ROS through NADPH oxidase. Ang II, angiotensin II; Ang-Tie, angiopoietin-Tie; AT1-R, angiotensin II type 1 (AT1) receptor; HIF-1α, hypoxia inducible factor-1α; NF-kB, nuclear factor kappa AB; ROS, reactive oxygen species; VSMCs, vascular smooth muscle cells.

Article Snippet: The primary antibodies were rabbit anti-human angiotensin II receptor type-1 polyclonal antibody (AAR-011, Alomone Labs, Jerusalem, Israel) at a dilution of 1:100 and rabbit anti-human angiotensin II type 2 receptor antibody (ab19134, AbCam, UK) at a dilution of 1:100.

Techniques: Binding Assay, Activation Assay