Journal: Asian Journal of Andrology
Article Title: Local renin angiotensin system and sperm DNA fragmentation
Figure Lengend Snippet: Schematic representation of the sperm renin angiotensin system, with the alterations (in bold) that occur in its axes in spermatozoa with DNA fragmentation. Due to the principal location of the enzymes and receptors altered in this principal axis of renin angiotensin system (RAS) (the tail of spermatozoa), it could be suggested that the spermatic motility is modified in DNA fragmented cells. ACE: angiotensin-converting enzyme; NEP: neutral endopeptidase; PEP: prolyl endopeptidase; MasR: angiotensin (A) 1–7 or Mas receptor; APN: aminopeptidase N; APA: aminopeptidase A; APB: aminopeptidase B; AT1R: A II receptor type 1; AT2R: A II receptor type 2; AT4R: A IV receptor; PRR: (pro)renin receptor.
Article Snippet: The samples were incubated in blocking buffer (PBS with 10% [w /v] fetal bovine serum [FBS]; Biochrom, Cambridge, UK) for 30 min and then with the following primary antibodies: anti-AT1R (extracellular) antibody (rabbit polyclonal antibody to AT1R; AAR011; Alomone Labs, Jerusalem, Israel), anti-AT2R antibody (rabbit polyclonal; ab19134; Abcam, Cambridge, UK), anti-AT4R antibody (Insulin-regulated aminopeptidase [IRAP]; rabbit polyclonal; clone H-133, sc-135229; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-renin receptor (rabbit polyclonal; clone H-85, sc-67390; Santa Cruz Biotechnology), anti-MasR (rabbit polyclonal; AAR-013; Alomone Labs), PE-labeled human ACE monoclonal phycoerythrin (PE mouse anti-human CD143; 344204; BioLegend, San Diego, CA, USA), PE anti-human CD13 (PE mouse anti-human CD13; 555394; BD Biosciences), and PE mouse anti-human CD10 (PE mouse anti-human CD10; 555375; BD Biosciences).