Rabbit Anti A2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti a2a/product/Alomone Labs
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1) Product Images from "A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence"
Article Title: A2A-D2 Heteromers on Striatal Astrocytes: Biochemical and Biophysical Evidence
Journal: International Journal of Molecular Sciences
Figure Legend Snippet: Interaction between A2A and D2 on rat striatal astrocyte processes: co-immunoprecipitation. ( A ) Aliquots (300 µg) of Triton X-100-soluble proteins obtained from gliosomes were immunoprecipitated with 1 µg of anti-A2A antibody as described in Methods. Immunoprecipitated (IP) and not immunoprecipitated (Output) materials were analyzed by immunoblotting using the anti-A2A antibody. IP and Output were also analyzed using anti-D2 antibody. A representative blot (of three) is shown. D2 immunoreactive bands were quantified and the data were reported in the graph. Values are means ± SEM ( n = 3). ( B ) Aliquots (300 µg) of Triton X-100-soluble proteins obtained from gliosomes were immunoprecipitated with 1 µg of anti-D2 antibody as described in Methods. IP and Output were analyzed by immunoblotting using the anti-D2 antibody. IP and Output were also analyzed using anti-A2A antibody. A representative blot (of three) is shown. A2A immunoreactive bands were quantified and the data were reported in the graph. Values are means ± SEM ( n = 3).
Techniques Used: Immunoprecipitation
Figure Legend Snippet: A2A-D2 heterodimers on rat striatal astrocytes: proximity ligation assay. Detection of in situ PLA A2A-D2 heteroreceptor complexes was carried out with primary antibodies (rabbit polyclonal anti-A2AR, mouse monoclonal anti-D2R and goat polyclonal anti-GFAP) in rat striatal slices. ( A ) The merge of the maximum intensity projections of a representative field (240x240 µm; z 10 µm) is shown; GFAP (red), DAPI (blue), PLA for A2A-D2 heteroreceptor complexes appears as yellow clusters. The boxed region (i) is shown at a higher magnification in ( B ). ( C ) Merged confocal images of a single z stack of the boxed region (ii) at a higher magnification: double immunolabeling for GFAP (red) and PLA A2A-D2 heteroreceptor complexes (green). ( D ) Colocalized map of the boxed region (ii); the colocalization analysis plugins—colocalization threshold with GFAP as ROI—was used to create the colocalized map; ImageJ Fiji software. A complete lack of stain for PLA A2A-D2 heteroreceptor complexes was obtained in the negative control experiments, performed avoiding the conjugation of a primary antibody with the Duolink Probes. In the figure the merges of the maximum intensity projections of two representative fields are shown: PLA for A2A-D2. heteroreceptor complexes without the primary anti-A2A antibody ( E ) or the primary anti-D2 antibody ( F ). Scale bar 25 µm or 10 µm
Techniques Used: Proximity Ligation Assay, In Situ, Immunolabeling, Software, Staining, Negative Control, Conjugation Assay