Journal: Evidence-based Complementary and Alternative Medicine : eCAM
Article Title: Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABAA Receptors in Rat Spinal Cord
Figure Lengend Snippet: Expression levels of GABA A , A1R, TRPV1, TRPV4, and mGluR3 receptors in rat SC. (a) GABA A , (b) A1R, (c) TRPV1, (d) TRPV4, and (e) mGluR3 expression levels in the SC of the rats from the sham, 2-Hz EA, and 15-Hz EA groups (from left to right). Sham: neuropathic pain rats with sham EA; 2 Hz: neuropathic pain rats that received 2-Hz EA; 15 Hz: neuropathic pain rats that received 15-Hz EA. ∗p
Article Snippet: The membrane was blocked with 5% nonfat milk in a TBST buffer (10 mmol/L Tris-buffered saline, pH 7.5; 100 mmol/L NaCl; and 0.1% Tween 20) and incubated overnight at 4°C with the anti-GABAA antibody (1:1000, Alomone, Israel), anti-A1R antibody (1:1000, Alomone), anti-TRPV1 (1:1000, Alomone), anti-TRPV4 antibody (1:1000, Alomone), and anti-GluR3 antibody (1:1000, Alomone) in TBST containing bovine serum albumin.