swell1  (Alomone Labs)


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    Structured Review

    Alomone Labs swell1
    Caveolar macromolecular mechanosensitive complex. Immunofluorescent analysis of colocalized expression of ClC‐3 (left), ClC‐2 (middle), and SWELL 1 (right) chloride channels with caveolae scaffolding protein Cav3 (caveolin 3) in nonstretched rat pulmonary vein distal ( PV dis ; A ) and human left atrium ( B ) myocardium. For colocalization analysis, intensity level of 30% was used as a threshold. Cav3 indicates caveolin 3; ClC, chloride channel; <t>SWELL1,</t> also known as LRRC8A (leucine rich repeat containing protein 8A).
    Swell1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/swell1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    swell1 - by Bioz Stars, 2022-07
    94/100 stars

    Images

    1) Product Images from "Caveolae‐Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis"

    Article Title: Caveolae‐Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.119.012748

    Caveolar macromolecular mechanosensitive complex. Immunofluorescent analysis of colocalized expression of ClC‐3 (left), ClC‐2 (middle), and SWELL 1 (right) chloride channels with caveolae scaffolding protein Cav3 (caveolin 3) in nonstretched rat pulmonary vein distal ( PV dis ; A ) and human left atrium ( B ) myocardium. For colocalization analysis, intensity level of 30% was used as a threshold. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).
    Figure Legend Snippet: Caveolar macromolecular mechanosensitive complex. Immunofluorescent analysis of colocalized expression of ClC‐3 (left), ClC‐2 (middle), and SWELL 1 (right) chloride channels with caveolae scaffolding protein Cav3 (caveolin 3) in nonstretched rat pulmonary vein distal ( PV dis ; A ) and human left atrium ( B ) myocardium. For colocalization analysis, intensity level of 30% was used as a threshold. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Techniques Used: Expressing, Scaffolding

    Co‐IP Western blots showing degree of association of Cav3 and chloride channels ClC‐2, ClC‐3, and SWELL 1 in rat pulmonary vein ( PV ; n=2). IB indicates immunoblot; IP , immunoprecipitation. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).
    Figure Legend Snippet: Co‐IP Western blots showing degree of association of Cav3 and chloride channels ClC‐2, ClC‐3, and SWELL 1 in rat pulmonary vein ( PV ; n=2). IB indicates immunoblot; IP , immunoprecipitation. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Techniques Used: Co-Immunoprecipitation Assay, Western Blot, Immunoprecipitation

    Molecular and structural remodeling of caveolar mechanosensitive complex. A , Immunoblots for ClC‐2, ClC‐3, SWELL 1, and Cav3 from Wistar ( WT ; n=4) and spontaneously hypertensive rat ( SHR ; n=4) pulmonary vein distal ( PV dis ) tissues. Expression levels were normalized to GAPDH . P values were determined by unpaired Student t test. B , Downregulation of Cav3 correlates with elevated membrane tension and disruption of caveolae structures during hypertension. Rep resentative composite electron micrographs showing the lateral sarcolemmal membranes of cardiomyocytes from nonstretched WT and SHR PV dis tissues. Blue arrowheads denote caveolae connected to plasma membrane (scale bars=200 nm). C and D , Quantification of caveolae density, normalized to membrane length ( C ), and membrane convolution index (L/L o −1) ( D ), n=40 cells per group. Box plots show medians with interquartile range; whiskers represent fifth and 95th percentile; each point represents 1 micrograph. P values were determined by unpaired Student t test. Cav3 indicates caveolin 3; ClC, chloride channel; L, length of membrane contour; L o , shortest length connecting end points of membrane segment; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).
    Figure Legend Snippet: Molecular and structural remodeling of caveolar mechanosensitive complex. A , Immunoblots for ClC‐2, ClC‐3, SWELL 1, and Cav3 from Wistar ( WT ; n=4) and spontaneously hypertensive rat ( SHR ; n=4) pulmonary vein distal ( PV dis ) tissues. Expression levels were normalized to GAPDH . P values were determined by unpaired Student t test. B , Downregulation of Cav3 correlates with elevated membrane tension and disruption of caveolae structures during hypertension. Rep resentative composite electron micrographs showing the lateral sarcolemmal membranes of cardiomyocytes from nonstretched WT and SHR PV dis tissues. Blue arrowheads denote caveolae connected to plasma membrane (scale bars=200 nm). C and D , Quantification of caveolae density, normalized to membrane length ( C ), and membrane convolution index (L/L o −1) ( D ), n=40 cells per group. Box plots show medians with interquartile range; whiskers represent fifth and 95th percentile; each point represents 1 micrograph. P values were determined by unpaired Student t test. Cav3 indicates caveolin 3; ClC, chloride channel; L, length of membrane contour; L o , shortest length connecting end points of membrane segment; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Techniques Used: Western Blot, Expressing

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    Alomone Labs swell1
    Caveolar macromolecular mechanosensitive complex. Immunofluorescent analysis of colocalized expression of ClC‐3 (left), ClC‐2 (middle), and SWELL 1 (right) chloride channels with caveolae scaffolding protein Cav3 (caveolin 3) in nonstretched rat pulmonary vein distal ( PV dis ; A ) and human left atrium ( B ) myocardium. For colocalization analysis, intensity level of 30% was used as a threshold. Cav3 indicates caveolin 3; ClC, chloride channel; <t>SWELL1,</t> also known as LRRC8A (leucine rich repeat containing protein 8A).
    Swell1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/swell1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    swell1 - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

    94
    Alomone Labs lrrc8a subunit
    <t>LRRC8A</t> protein is increased in the hippocampus of KA + SAL group. ( A ) Sample film, ( B ) Calibration plot (lines fitted by linear least-squares regression) and ( C ) Mean levels (mean ± sem). Each lane corresponds to one sample. Lanes 1–5 are from CON + SAL group; lanes 6–10 from KA + SAL group and lanes 11–15 from KA + MI group. Lanes IS-1-IS4 internal standards containing, respectively, 15, 30, 45, and 60 μg protein. The details of statistical analysis are provided in the manuscript.
    Lrrc8a Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lrrc8a subunit/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lrrc8a subunit - by Bioz Stars, 2022-07
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    93
    Alomone Labs l type ca2 channel α1c subunit
    <t>LRRC8A</t> protein is increased in the hippocampus of KA + SAL group. ( A ) Sample film, ( B ) Calibration plot (lines fitted by linear least-squares regression) and ( C ) Mean levels (mean ± sem). Each lane corresponds to one sample. Lanes 1–5 are from CON + SAL group; lanes 6–10 from KA + SAL group and lanes 11–15 from KA + MI group. Lanes IS-1-IS4 internal standards containing, respectively, 15, 30, 45, and 60 μg protein. The details of statistical analysis are provided in the manuscript.
    L Type Ca2 Channel α1c Subunit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type ca2 channel α1c subunit/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type ca2 channel α1c subunit - by Bioz Stars, 2022-07
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    Image Search Results


    Caveolar macromolecular mechanosensitive complex. Immunofluorescent analysis of colocalized expression of ClC‐3 (left), ClC‐2 (middle), and SWELL 1 (right) chloride channels with caveolae scaffolding protein Cav3 (caveolin 3) in nonstretched rat pulmonary vein distal ( PV dis ; A ) and human left atrium ( B ) myocardium. For colocalization analysis, intensity level of 30% was used as a threshold. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Caveolae‐Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis

    doi: 10.1161/JAHA.119.012748

    Figure Lengend Snippet: Caveolar macromolecular mechanosensitive complex. Immunofluorescent analysis of colocalized expression of ClC‐3 (left), ClC‐2 (middle), and SWELL 1 (right) chloride channels with caveolae scaffolding protein Cav3 (caveolin 3) in nonstretched rat pulmonary vein distal ( PV dis ; A ) and human left atrium ( B ) myocardium. For colocalization analysis, intensity level of 30% was used as a threshold. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Article Snippet: Immunohistochemistry Masson's trichrome staining and double‐immunolabeling for ClC‐2 (goat polyclonal; SAB2501373, Sigma‐Aldrich), ClC‐3 (rabbit polyclonal; ACL‐001, Alomone Labs), SWELL1 (anti‐LRRC8A, rabbit polyclonal; AAC‐001, Alomone Labs), and Cav3 (mouse monoclonal; 610421, BD Biosciences; rabbit polyclonal; ab2912, Abcam) were performed on paraffin‐embedded sections of unstretched PV preparations.

    Techniques: Expressing, Scaffolding

    Co‐IP Western blots showing degree of association of Cav3 and chloride channels ClC‐2, ClC‐3, and SWELL 1 in rat pulmonary vein ( PV ; n=2). IB indicates immunoblot; IP , immunoprecipitation. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Caveolae‐Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis

    doi: 10.1161/JAHA.119.012748

    Figure Lengend Snippet: Co‐IP Western blots showing degree of association of Cav3 and chloride channels ClC‐2, ClC‐3, and SWELL 1 in rat pulmonary vein ( PV ; n=2). IB indicates immunoblot; IP , immunoprecipitation. Cav3 indicates caveolin 3; ClC, chloride channel; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Article Snippet: Immunohistochemistry Masson's trichrome staining and double‐immunolabeling for ClC‐2 (goat polyclonal; SAB2501373, Sigma‐Aldrich), ClC‐3 (rabbit polyclonal; ACL‐001, Alomone Labs), SWELL1 (anti‐LRRC8A, rabbit polyclonal; AAC‐001, Alomone Labs), and Cav3 (mouse monoclonal; 610421, BD Biosciences; rabbit polyclonal; ab2912, Abcam) were performed on paraffin‐embedded sections of unstretched PV preparations.

    Techniques: Co-Immunoprecipitation Assay, Western Blot, Immunoprecipitation

    Molecular and structural remodeling of caveolar mechanosensitive complex. A , Immunoblots for ClC‐2, ClC‐3, SWELL 1, and Cav3 from Wistar ( WT ; n=4) and spontaneously hypertensive rat ( SHR ; n=4) pulmonary vein distal ( PV dis ) tissues. Expression levels were normalized to GAPDH . P values were determined by unpaired Student t test. B , Downregulation of Cav3 correlates with elevated membrane tension and disruption of caveolae structures during hypertension. Rep resentative composite electron micrographs showing the lateral sarcolemmal membranes of cardiomyocytes from nonstretched WT and SHR PV dis tissues. Blue arrowheads denote caveolae connected to plasma membrane (scale bars=200 nm). C and D , Quantification of caveolae density, normalized to membrane length ( C ), and membrane convolution index (L/L o −1) ( D ), n=40 cells per group. Box plots show medians with interquartile range; whiskers represent fifth and 95th percentile; each point represents 1 micrograph. P values were determined by unpaired Student t test. Cav3 indicates caveolin 3; ClC, chloride channel; L, length of membrane contour; L o , shortest length connecting end points of membrane segment; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Caveolae‐Mediated Activation of Mechanosensitive Chloride Channels in Pulmonary Veins Triggers Atrial Arrhythmogenesis

    doi: 10.1161/JAHA.119.012748

    Figure Lengend Snippet: Molecular and structural remodeling of caveolar mechanosensitive complex. A , Immunoblots for ClC‐2, ClC‐3, SWELL 1, and Cav3 from Wistar ( WT ; n=4) and spontaneously hypertensive rat ( SHR ; n=4) pulmonary vein distal ( PV dis ) tissues. Expression levels were normalized to GAPDH . P values were determined by unpaired Student t test. B , Downregulation of Cav3 correlates with elevated membrane tension and disruption of caveolae structures during hypertension. Rep resentative composite electron micrographs showing the lateral sarcolemmal membranes of cardiomyocytes from nonstretched WT and SHR PV dis tissues. Blue arrowheads denote caveolae connected to plasma membrane (scale bars=200 nm). C and D , Quantification of caveolae density, normalized to membrane length ( C ), and membrane convolution index (L/L o −1) ( D ), n=40 cells per group. Box plots show medians with interquartile range; whiskers represent fifth and 95th percentile; each point represents 1 micrograph. P values were determined by unpaired Student t test. Cav3 indicates caveolin 3; ClC, chloride channel; L, length of membrane contour; L o , shortest length connecting end points of membrane segment; SWELL1, also known as LRRC8A (leucine rich repeat containing protein 8A).

    Article Snippet: Immunohistochemistry Masson's trichrome staining and double‐immunolabeling for ClC‐2 (goat polyclonal; SAB2501373, Sigma‐Aldrich), ClC‐3 (rabbit polyclonal; ACL‐001, Alomone Labs), SWELL1 (anti‐LRRC8A, rabbit polyclonal; AAC‐001, Alomone Labs), and Cav3 (mouse monoclonal; 610421, BD Biosciences; rabbit polyclonal; ab2912, Abcam) were performed on paraffin‐embedded sections of unstretched PV preparations.

    Techniques: Western Blot, Expressing

    LRRC8A protein is increased in the hippocampus of KA + SAL group. ( A ) Sample film, ( B ) Calibration plot (lines fitted by linear least-squares regression) and ( C ) Mean levels (mean ± sem). Each lane corresponds to one sample. Lanes 1–5 are from CON + SAL group; lanes 6–10 from KA + SAL group and lanes 11–15 from KA + MI group. Lanes IS-1-IS4 internal standards containing, respectively, 15, 30, 45, and 60 μg protein. The details of statistical analysis are provided in the manuscript.

    Journal: International Journal of Molecular Sciences

    Article Title: Myo-Inositol Limits Kainic Acid-Induced Epileptogenesis in Rats

    doi: 10.3390/ijms23031198

    Figure Lengend Snippet: LRRC8A protein is increased in the hippocampus of KA + SAL group. ( A ) Sample film, ( B ) Calibration plot (lines fitted by linear least-squares regression) and ( C ) Mean levels (mean ± sem). Each lane corresponds to one sample. Lanes 1–5 are from CON + SAL group; lanes 6–10 from KA + SAL group and lanes 11–15 from KA + MI group. Lanes IS-1-IS4 internal standards containing, respectively, 15, 30, 45, and 60 μg protein. The details of statistical analysis are provided in the manuscript.

    Article Snippet: Antibodies against Glial fibrillary acidic protein (GFAP), LRRC8A subunit of volume regulated anionic channel (VRAC), protein tyrosine phosphatase receptor type R (PTPRR) and Doublecortin reacted with the protein bands of the following molecular weight 50 KDa, 75 KDa, 75 KDa and 45 KDa correspondingly ( , , and ).

    Techniques:

    LRRC8A protein is increased in the hippocampus of KA + SAL group. ( A ) Sample film, ( B ) Calibration plot (lines fitted by linear least-squares regression) and ( C ) Mean levels (mean ± sem). Each lane corresponds to one sample. Lanes 1–5 are from CON + SAL group; lanes 6–10 from KA + SAL group and lanes 11–15 from KA + MI group. Lanes IS-1-IS4 internal standards containing, respectively, 15, 30, 45, and 60 μg protein. The details of statistical analysis are provided in the manuscript.

    Journal: International Journal of Molecular Sciences

    Article Title: Myo-Inositol Limits Kainic Acid-Induced Epileptogenesis in Rats

    doi: 10.3390/ijms23031198

    Figure Lengend Snippet: LRRC8A protein is increased in the hippocampus of KA + SAL group. ( A ) Sample film, ( B ) Calibration plot (lines fitted by linear least-squares regression) and ( C ) Mean levels (mean ± sem). Each lane corresponds to one sample. Lanes 1–5 are from CON + SAL group; lanes 6–10 from KA + SAL group and lanes 11–15 from KA + MI group. Lanes IS-1-IS4 internal standards containing, respectively, 15, 30, 45, and 60 μg protein. The details of statistical analysis are provided in the manuscript.

    Article Snippet: Optical density was proportional to the amounts of GFAP, LRRC8A subunit of VRAC, PTPRR and Doublecortin (see B,E, B, B and B).

    Techniques: