rabbit anti ova igg (Worthington Biochemical)

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Name:

Papain Dissociation System

Description:

Set of five single use vials of papain and five single use vials of DNase 100 ml of Earle s balanced salt solution EBSS and an inhibitor vial for use in the tissue dissociation method of Huettner J and Baughman R J Neuroscience 6 3044 1986 Use tested by Worthington using new born rat pup spinal cord The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0 3 0 4 cm3 each

Catalog Number:

lk003150

Price:

240

Size:

1 bx

Cas Number:

see components

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Structured Review

Worthington Biochemical rabbit anti ova igg

Mφ-II up-regulate SPHK1 and TNFSF14/LIGHT. (A) Relative SPHK1 mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + <t>IgG-OVA</t> IC (▲), or IL-4 (○). (B) Relative LIGHT mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). Calculation of fold values was detailed in Materials and Methods. (C) Immunoprecipitation of soluble LIGHT (sLIGHT) from 6 h cell supernatants of unstimulated Mφ, Ca-Mφ, and Mφ-II. Western blot analysis for LIGHT using a rat α-LIGHT mAb, showing the soluble form of LIGHT present as a 20- to 23-kD protein. Figures are representative of at least three independent experiments.

rabbit anti ova igg - by Bioz Stars, 2020-05

88/100 stars

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Images

1) Product Images from "Biochemical and functional characterization of three activated macrophage populations"

Article Title: Biochemical and functional characterization of three activated macrophage populations

Journal: Journal of leukocyte biology

doi: 10.1189/jlb.0406249

Mφ-II up-regulate SPHK1 and TNFSF14/LIGHT. (A) Relative SPHK1 mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). (B) Relative LIGHT mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). Calculation of fold values was detailed in Materials and Methods. (C) Immunoprecipitation of soluble LIGHT (sLIGHT) from 6 h cell supernatants of unstimulated Mφ, Ca-Mφ, and Mφ-II. Western blot analysis for LIGHT using a rat α-LIGHT mAb, showing the soluble form of LIGHT present as a 20- to 23-kD protein. Figures are representative of at least three independent experiments.

Figure Legend Snippet: Mφ-II up-regulate SPHK1 and TNFSF14/LIGHT. (A) Relative SPHK1 mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). (B) Relative LIGHT mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). Calculation of fold values was detailed in Materials and Methods. (C) Immunoprecipitation of soluble LIGHT (sLIGHT) from 6 h cell supernatants of unstimulated Mφ, Ca-Mφ, and Mφ-II. Western blot analysis for LIGHT using a rat α-LIGHT mAb, showing the soluble form of LIGHT present as a 20- to 23-kD protein. Figures are representative of at least three independent experiments.

Techniques Used: Real-time Polymerase Chain Reaction, Activation Assay, Immunoprecipitation, Western Blot

2) Product Images from "Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity"

Article Title: Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

Journal: Immunity

doi: 10.1016/j.immuni.2013.10.006

Figure Legend Snippet: PLA2 induces a Th2 response through cleavage of membrane phospholipids to produce lysophospholipids

Techniques Used:

Flow Cytometry:

Article Title: Analysis of Notch Signaling-Dependent Gene Expression in Developing Airways Reveals Diversity of Clara Cells
Article Snippet: .. Flow Cytometry and Cell Sorting Tracheas and lungs from B1-GFP mice were separated, cut into pieces and then incubated in papain dissociation solution according to the manual (LK003153, Worthington Biochemical Corporation). ..

Selection:

Article Title: Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration
Article Snippet: .. To isolate rod or cone photoreceptors from the retina, we used the Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ) following a previously described modified protocol , followed by EasySep Mouse PE or FITC positive selection kit (Stem Cell Technologies, Vancouver, Canada), respectively, following the manufacturer’s protocol. .. We used 1 μg/ml of PE-conjugated anti-CD73 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) ( ) for rod photoreceptors and 5 μg/ml of fluorescein peanut agglutinin (Vector Laboratories, Burlingame, CA) ( ) for cone photoreceptors, respectively.

Fluorescence:

Article Title: Brain Endothelial Cells Control Fertility through Ovarian-Steroid-Dependent Release of Semaphorin 3A
Article Snippet: .. Fluorescence-Activated Cell-Sorting Analysis MEs from female P90 rats (n = 3) were microdissected and enzymatically dissociated using a Papain Dissociation System (Worthington, Lakewood, NJ) to obtain single-cell suspensions. .. Subsequently, dissociated cells were resuspended in Hanks' balanced salt solution (HBSS; Invitrogen) containing 1% BSA.

Cytometry:

Mouse Assay:

Incubation:

Article Title: Alteration of genic 5-hydroxymethylcytosine patterning in olfactory neurons correlates with changes in gene expression and cell identity
Article Snippet: .. MOE were dissected into Earl’s Buffered Saline Solution, minced in Papain dissociation solution (Worthington), and incubated at 37 °C for 45 min. ..