rabbit anti ova igg (Worthington Biochemical)

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Name:

Deoxyribonuclease I

Description:

Chromatographically purified A lyophilized powder with glycine as a stabilizer

Catalog Number:

ls002004

Price:

Size:

5 mg

Source:

Bovine Pancreas

Cas Number:

9003.98.9

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Structured Review

Worthington Biochemical rabbit anti ova igg

Mφ-II up-regulate SPHK1 and TNFSF14/LIGHT. (A) Relative SPHK1 mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + <t>IgG-OVA</t> IC (▲), or IL-4 (○). (B) Relative LIGHT mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). Calculation of fold values was detailed in Materials and Methods. (C) Immunoprecipitation of soluble LIGHT (sLIGHT) from 6 h cell supernatants of unstimulated Mφ, Ca-Mφ, and Mφ-II. Western blot analysis for LIGHT using a rat α-LIGHT mAb, showing the soluble form of LIGHT present as a 20- to 23-kD protein. Figures are representative of at least three independent experiments.

Chromatographically purified A lyophilized powder with glycine as a stabilizer
https://www.bioz.com/result/rabbit anti ova igg/product/Worthington Biochemical
Average 88 stars, based on 1824 article reviews
Price from $9.99 to $1999.99

rabbit anti ova igg - by Bioz Stars, 2020-08

88/100 stars

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Images

1) Product Images from "Biochemical and functional characterization of three activated macrophage populations"

Article Title: Biochemical and functional characterization of three activated macrophage populations

Journal: Journal of leukocyte biology

doi: 10.1189/jlb.0406249

Mφ-II up-regulate SPHK1 and TNFSF14/LIGHT. (A) Relative SPHK1 mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). (B) Relative LIGHT mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). Calculation of fold values was detailed in Materials and Methods. (C) Immunoprecipitation of soluble LIGHT (sLIGHT) from 6 h cell supernatants of unstimulated Mφ, Ca-Mφ, and Mφ-II. Western blot analysis for LIGHT using a rat α-LIGHT mAb, showing the soluble form of LIGHT present as a 20- to 23-kD protein. Figures are representative of at least three independent experiments.

Figure Legend Snippet: Mφ-II up-regulate SPHK1 and TNFSF14/LIGHT. (A) Relative SPHK1 mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). (B) Relative LIGHT mRNA as measured by real-time PCR after Mφ activation by LPS (●), LPS + IgG-OVA IC (▲), or IL-4 (○). Calculation of fold values was detailed in Materials and Methods. (C) Immunoprecipitation of soluble LIGHT (sLIGHT) from 6 h cell supernatants of unstimulated Mφ, Ca-Mφ, and Mφ-II. Western blot analysis for LIGHT using a rat α-LIGHT mAb, showing the soluble form of LIGHT present as a 20- to 23-kD protein. Figures are representative of at least three independent experiments.

Techniques Used: Real-time Polymerase Chain Reaction, Activation Assay, Immunoprecipitation, Western Blot

2) Product Images from "Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity"

Article Title: Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

Journal: Immunity

doi: 10.1016/j.immuni.2013.10.006

Figure Legend Snippet: PLA2 induces a Th2 response through cleavage of membrane phospholipids to produce lysophospholipids

Techniques Used:

Produced:

Article Title: Novel High-Throughput Deoxyribonuclease 1 Assay
Article Snippet: .. The percentage of DNase I activity was calculated using Equation 1: DNase\u00a0I\u00a0activity (%) =\u00a0 (mean\u00a0velocity\u00a0of\u00a0a\u00a0compound/mean\u00a0velocity\u00a0of\u00a0DMSO)\u00a0\u00d7\u00a0100 (1) In similar assays, recombinant murine EndoG (produced in-house) was used at a concentration of 0.14 μM in 0.1 mM MgCl2 , 10 mM Tris-HCl, pH 7.4; and DNase II (Worthington, Lakewood, NJ) (3.32 nM) was tested in 100 mM sodium citrate buffer, pH 5.0. .. For evaluation of the quality of the assay, Z’ values were calculated using Equation 2: Z\u2019 =\u00a01\u00a0\u2212\u00a0(3SDC +\u00a03SDB )/(MC \u00a0\u2212\u00a0MB ) (2) where M = mean value; SD = standard deviation; C = control; and B = background.

Concentration Assay:

other:

Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
Article Snippet: The role of DNase I in SEL and AMI has been previously investigated, however at present to the best of our knowledge, no correlative studies on its association with type 2 diabetes have been conducted ( , , ).

Activity Assay:

Expressing:

Article Title: DNase I aggravates islet β-cell apoptosis in type 2 diabetes
Article Snippet: .. In order to confirm that apoptosis was correlated with DNase I expression, siRNA was used to knock down DNase I. .. It was observed that a reduction in DNase I expression resulted in significant reductions in apoptotic rate and caspase-3 protein levels, even in the presence of high glucose.

Staining:

Article Title: Constitutive Nucleosome Depletion and Ordered Factor Assembly at the GRP78 Promoter Revealed by Single Molecule Footprinting
Article Snippet: .. These were then digested at 37 °C for 15 min using various concentrations of DNase I (Worthington, San Francisco, California, United States) to obtain a suitable range of digestion of genomic DNA as revealed by EtBr staining. .. Digested genomic DNA was purified, redigested by RsaI, resolved on a 1.5% agarose gel, and Southern blotted.