sphk1 antibody (Boster Bio)
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94
Structured Review
Boster Bio
sphk1 antibody
Sphk1 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk1 antibody/product/Boster Bio
Average 94 stars, based on 6 article reviews
Sphk1 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk1 antibody/product/Boster Bio
Average 94 stars, based on 6 article reviews
sphk1 antibody - by Bioz Stars,
2026-02
94/100 stars
Images
1) Product Images from "Sphingosine kinase 1 promotes M2 macrophage infiltration and enhances glioma cell migration via the JAK2/STAT3 pathway"
Article Title: Sphingosine kinase 1 promotes M2 macrophage infiltration and enhances glioma cell migration via the JAK2/STAT3 pathway
Journal: Scientific Reports
doi: 10.1038/s41598-025-88328-2
Figure Legend Snippet: Overall survival curves of SPHK1 in different databases. ( A – I ) Aberrant high expression of SPHK1 leads to a worse prognosis. ( J ) A meta-analysis of these databases yielded mean HR values for SPHK1. The experiment was performed thrice, and the results are presented as mean and standard deviation. Statistical significance was determined at p < 0.05.
Techniques Used: Expressing, Standard Deviation
Figure Legend Snippet: Correlation analysis between SPHK1 and clinical indicators. These clinical indicators were WHO Grade, 2021 Classification, IDH status, and 1p19q status, where ( A ) analyzes TCGA, ( B ) CGGA, and ( C ) Gravendeel databases. Statistical significance was determined at p < 0.05.
Techniques Used:
Figure Legend Snippet: Univariate and multivariate prognostic analysis of SPHK1. ( A – B ) TCGA, ( C – D ) CGGA, and ( E – F ) Gravendeel databases. Statistical significance was determined at p < 0.05.
Techniques Used:
Figure Legend Snippet: Relationship between SPHK1 and the immune microenvironment of gliomas. ( A ) Correlation of SPHK1 with 24 immune cells. ( B ) SPHK1 expression is associated with multiple immune cell types. ( C ) Relationship between SPHK1 and macrophages. ( D ) SPHK1 and neutrophils. ( E ) SPHK1 and Th2 cells. ( F ) Relationship between SPHK1 and immunity in innate, adaptive, and cancer-immunity cycle. ( G ) Enrichment scores of the cancer-immunity cycle and SPHK1 expression levels. Statistical significance was determined at p < 0.05.
Techniques Used: Expressing
Figure Legend Snippet: Determination of SPHK1 expression levels and knockdown versus overexpression. ( A ) Immunohistochemistry reveals SPHK1 expression levels in glioma tissues of different WHO grades. ( B ) Expression levels of SPHK1 in HA versus different glioma cell lines. ( C ) Knockdown and overexpression of SPHK1 in U251 and T98G cell lines. The experiment was performed thrice, and the results are presented as mean and standard deviation. Statistical significance was determined at p < 0.05.
Techniques Used: Expressing, Knockdown, Over Expression, Immunohistochemistry, Standard Deviation
Figure Legend Snippet: Expression level of SPHK1 affects the migration ability of glioma cells. ( A ) Transwell assay reveals SPHK1 affects glioma cell migration. ( B ) Wound healing assay reveals SPHK1 affects glioma cell migration. The experiment was performed thrice, and the results are presented as mean and standard deviation. Statistical significance was determined at p < 0.05.
Techniques Used: Expressing, Migration, Transwell Assay, Wound Healing Assay, Standard Deviation
Figure Legend Snippet: SPHK1 affects M2 macrophage infiltration in the glioma microenvironment. ( A ) Morphologic changes in the induction of THP-1 cells to M2 macrophages. ( B ) RT-qPCR validation of markers for M2 macrophages. ( C ) Schematic representation of the M2 macrophage infiltration assay. ( D ) SPHK1 expression level affects the number of M2 macrophage infiltrations. ( E ) JAK2/STAT3 pathway is inhibited upon SPHK1 knockdown and activated upon SPHK1 overexpression. The experiment was performed thrice, and the results are presented as mean and standard deviation. Statistical significance was determined at p < 0.05.
Techniques Used: Quantitative RT-PCR, Biomarker Discovery, Expressing, Knockdown, Over Expression, Standard Deviation
Figure Legend Snippet: SPHK1 facilitates glioma progress by the JAK2/STAT3 pathway. ( A – B ) The JAK2/STAT3 pathway inhibitor AG490 reverses the overactivation of glioma cell migration ability due to SPHK1 overexpression. ( C ) AG490 reversed the abnormal elevation of p-JAK2/JAK2 and p-STAT3/STAT3 induced by SPHK1 overexpression. The experiment was performed thrice, and the results are presented as mean and standard deviation. Statistical significance was determined at p < 0.05.
Techniques Used: Migration, Over Expression, Standard Deviation
Figure Legend Snippet: In vivo experiment. ( A – B ) Results in nude mice after anesthesia for tumor formation and after tumor stripping. ( C ) Volume and weight were measured after tumor removal. ( D ) Western blotting experiments in vivo illustrated that AG490 reversed the SPHK1 overexpression-induced elevation of p-JAK2/JAK2 and p-STAT3/STAT3. ( E ) CD206 and TGF-β1 expression level were detected after extracting RNA from tumor tissues. The experiment was performed thrice, and the results are presented as mean and standard deviation. Statistical significance was determined at p < 0.05.
Techniques Used: In Vivo, Stripping Membranes, Western Blot, Over Expression, Expressing, Standard Deviation