a23187  (Alomone Labs)


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    Alomone Labs a23187
    Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM <t>A23187.</t> After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P
    A23187, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a23187/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a23187 - by Bioz Stars, 2022-07
    90/100 stars

    Images

    1) Product Images from "A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells"

    Article Title: A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S168149

    Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P
    Figure Legend Snippet: Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P

    Techniques Used: Conditioned Place Preference, Incubation, Recombinant

    2) Product Images from "MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis"

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064551

    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p
    Figure Legend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Techniques Used: Labeling, Incubation

    3) Product Images from "MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis"

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064551

    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p
    Figure Legend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Techniques Used: Labeling, Incubation

    4) Product Images from "Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation"

    Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

    Journal: Experimental Animals

    doi: 10.1538/expanim.15-0004

    Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.
    Figure Legend Snippet: Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.

    Techniques Used:

    Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P
    Figure Legend Snippet: Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P

    Techniques Used: Permeability, Injection

    5) Product Images from "A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells"

    Article Title: A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S168149

    Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P
    Figure Legend Snippet: Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P

    Techniques Used: Incubation, Recombinant

    6) Product Images from "Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation"

    Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

    Journal: Experimental Animals

    doi: 10.1538/expanim.15-0004

    Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.
    Figure Legend Snippet: Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.

    Techniques Used:

    Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P
    Figure Legend Snippet: Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P

    Techniques Used: Permeability, Injection

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    Alomone Labs αa conotoxin piva
    The relative effect of specific α7-nAChR antagonists to block ACh (10 −5 M) elicited responses. A- αBgTx attenuated the ACh evoked contractions in a concentration dependent manner. The blocking effects of 50 nM and 100 nM toxin were statistically significant. The recovery is almost complete. B- The block by 50 nM <t>αA-CTx</t> <t>PIVA</t> was not significant, while the α-CTx ImI at 100 nM blocked almost completely the ACh elicited contraction. The recovery from block was partial. n = number of muscles. Asterisks indicate a significant difference from the control value: *P
    αa Conotoxin Piva, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/αa conotoxin piva/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    αa conotoxin piva - by Bioz Stars, 2022-07
    85/100 stars
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    90
    Alomone Labs a23187
    Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM <t>A23187.</t> After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P
    A23187, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a23187/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a23187 - by Bioz Stars, 2022-07
    90/100 stars
      Buy from Supplier

    94
    Alomone Labs anti trpc1
    CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), <t>TRPC1</t> (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p
    Anti Trpc1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpc1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpc1 - by Bioz Stars, 2022-07
    94/100 stars
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    Image Search Results


    The relative effect of specific α7-nAChR antagonists to block ACh (10 −5 M) elicited responses. A- αBgTx attenuated the ACh evoked contractions in a concentration dependent manner. The blocking effects of 50 nM and 100 nM toxin were statistically significant. The recovery is almost complete. B- The block by 50 nM αA-CTx PIVA was not significant, while the α-CTx ImI at 100 nM blocked almost completely the ACh elicited contraction. The recovery from block was partial. n = number of muscles. Asterisks indicate a significant difference from the control value: *P

    Journal: PLoS ONE

    Article Title: Nicotinic Acetylcholine Receptors Containing the α7-Like Subunit Mediate Contractions of Muscles Responsible for Space Positioning of the Snail, Helix pomatia L. Tentacle

    doi: 10.1371/journal.pone.0109538

    Figure Lengend Snippet: The relative effect of specific α7-nAChR antagonists to block ACh (10 −5 M) elicited responses. A- αBgTx attenuated the ACh evoked contractions in a concentration dependent manner. The blocking effects of 50 nM and 100 nM toxin were statistically significant. The recovery is almost complete. B- The block by 50 nM αA-CTx PIVA was not significant, while the α-CTx ImI at 100 nM blocked almost completely the ACh elicited contraction. The recovery from block was partial. n = number of muscles. Asterisks indicate a significant difference from the control value: *P

    Article Snippet: The α-bungarotoxin (αBgTx), α-conotoxin ImI (α-CTx IMI) and αA-conotoxin PIVA (αA-CTx PIVA) were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Blocking Assay, Concentration Assay

    Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P

    Journal: International Journal of Nanomedicine

    Article Title: A TEM-traceable physiologically functional gold nanoprobe that permeates non-endocytic cells

    doi: 10.2147/IJN.S168149

    Figure Lengend Snippet: Membrane-permeable CPP-MT and CPP-MT-AuNP inhibit acrosomal exocytosis in sperm. Notes: Human sperm were incubated for 1 hour at 37°C in HTF medium with increasing concentrations of recombinant CPP-MT unconjugated ( A ) or conjugated to AuNPs ( B ). Afterward, the cells were incubated for 15 minutes without stimulus (control) or with 10 µM A23187. After incubation, the samples were fixed and the acrosomal exocytosis index was evaluated using PSL-FITC and calculated as detailed in section “Materials and methods”. The data represent mean values ± SEM and were calculated from three independent experiments. Two-way ANOVA shows statistically significant difference (*** P

    Article Snippet: A23187 was from Alomone Labs.

    Techniques: Conditioned Place Preference, Incubation, Recombinant

    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Journal: PLoS ONE

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

    doi: 10.1371/journal.pone.0064551

    Figure Lengend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Article Snippet: When data were normalized against MARCKS, similar results were obtained (see for the effect of A23187; the results for PMA and progesterone are not shown).

    Techniques: Labeling, Incubation

    CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p

    Journal: The Journal of allergy and clinical immunology

    Article Title: Mast Cell CRF2 Suppresses Mast Cell Degranulation and Limits the Severity of Anaphylaxis and Stress-Induced Intestinal Permeability

    doi: 10.1016/j.jaci.2018.08.053

    Figure Lengend Snippet: CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p

    Article Snippet: The membranes were blocked with 5% w/v BSA in Tris-buffered saline (TBS) with 0.1% Tween 20 (TBS-T) for 1 hour at room temperature, washed in TBS-T, incubated with the following Rabbit Anti-Human antibodies: STIM1 (alomone labs #ACC-063;1:500 dilution), Anti-TRPC1 (alomone labs #ACC-010;1:600 dilution), Anti-Orai1 (alomone labs #ACC-060;1:400 dilution), and β-actin (cell signaling, 4970, 1:1000) diluted in 5% w/v BSA in TBS-T for overnight at 4°C or 2 hours at room temperature, and then washed with TBS-T.

    Techniques: Expressing, Derivative Assay, Mouse Assay, Fluorescence, Western Blot, Two Tailed Test