a23187  (Alomone Labs)


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    Name:
    A23187
    Description:
    A23187 is a mobile carrier Ca2 ionophore and can cause cell activation differentiation or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors Furthermore A23187 induces in a concentration and time dependent manner Ca2 triggered cell apoptosis
    Catalog Number:
    A-600
    Price:
    300.0
    Category:
    Small Molecule
    Source:
    Streptomyces chartreusis.
    Applications:
    0
    Purity:
    >98%
    Size:
    1 Vials containing 5 mg each
    Format:
    Lyophilized/solid.
    Formula:
    C29H37N3O6
    Molecular Weight:
    523.6
    Molecule Name:
    5-(Methylamino)-2-[[2R,3R,6S,8S,9R,11R)-3,9,11- trimethy l-8-[(1S)-1-methyl-2-oxo-2-(1H-pyrrol-2-yl)-ethyl]-1,7- dioxaspiro[5.5]undec-2-yl]methyl]-4-benzoxazolecarboxylic acid.
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    Structured Review

    Alomone Labs a23187
    A23187
    A23187 is a mobile carrier Ca2 ionophore and can cause cell activation differentiation or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors Furthermore A23187 induces in a concentration and time dependent manner Ca2 triggered cell apoptosis
    https://www.bioz.com/result/a23187/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a23187 - by Bioz Stars, 2021-09
    91/100 stars

    Images

    1) Product Images from "MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis"

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064551

    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p
    Figure Legend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Techniques Used: Labeling, Incubation

    2) Product Images from "MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis"

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064551

    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p
    Figure Legend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Techniques Used: Labeling, Incubation

    3) Product Images from "Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation"

    Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

    Journal: Experimental Animals

    doi: 10.1538/expanim.15-0004

    Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.
    Figure Legend Snippet: Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.

    Techniques Used:

    Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P
    Figure Legend Snippet: Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P

    Techniques Used: Permeability, Injection

    4) Product Images from "Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation"

    Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation

    Journal: Experimental Animals

    doi: 10.1538/expanim.15-0004

    Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.
    Figure Legend Snippet: Illustration of main pathways to skin response via mast cells based on results shown in Fig. 4 . Nonresponder dogs showed skin responses to HCO-60, histamine dihydrochloride, concanavalin A, and A23187 comparable to those of wild-type dogs but no response to compound 48/80; the dysfunctional route in NR dogs would be downstream of the GPCR.

    Techniques Used:

    Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P
    Figure Legend Snippet: Vascular permeability to various compounds. Compound 48/80 (A), HCO-60 (B), histamine dihydrochloride (C), concanavalin A (D), and A23187 (E) at 0.01–100 mg/ml ID injected at 0.05 ml/site into the shaved thorax of anesthetized wild-type (WT) and nonresponder (NR) dogs; Evans blue (1 mg/kg) dissolved in saline IV injected just prior to ID injections; and 10 min later, ID injection sites collected for determination of pigment leakage. The significance of the differences between WT and NR groups was determined by Student’s t -test. ** P

    Techniques Used: Permeability, Injection

    Related Articles

    other:

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis
    Article Snippet: When data were normalized against MARCKS, similar results were obtained (see for the effect of A23187; the results for PMA and progesterone are not shown).

    Article Title: Novel phenotype in beagle dogs characterized by skin response to compound 48/80 focusing on skin mast cell degranulation
    Article Snippet: ChemicalsCompound 48/80 was purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA), HCO-60 was purchased from Nihon Surfactant Kogyo K.K. (Tokyo, Japan), histamine dihydrochloride was purchased from Nacalai Tesque, Inc. (Kyoto, Japan), Concanavalin A was purchased from Seikagaku Corp. (Tokyo, Japan), and A23187 was purchased from Alomone Labs, Ltd.. (Jerusalem, Israel).

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  • 85
    Alomone Labs αa conotoxin piva
    The relative effect of specific α7-nAChR antagonists to block ACh (10 −5 M) elicited responses. A- αBgTx attenuated the ACh evoked contractions in a concentration dependent manner. The blocking effects of 50 nM and 100 nM toxin were statistically significant. The recovery is almost complete. B- The block by 50 nM <t>αA-CTx</t> <t>PIVA</t> was not significant, while the α-CTx ImI at 100 nM blocked almost completely the ACh elicited contraction. The recovery from block was partial. n = number of muscles. Asterisks indicate a significant difference from the control value: *P
    αa Conotoxin Piva, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/αa conotoxin piva/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    αa conotoxin piva - by Bioz Stars, 2021-09
    85/100 stars
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    93
    Alomone Labs anti trpc1
    CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), <t>TRPC1</t> (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p
    Anti Trpc1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpc1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti trpc1 - by Bioz Stars, 2021-09
    93/100 stars
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    91
    Alomone Labs a23187
    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore <t>A23187</t> (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p
    A23187, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a23187/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a23187 - by Bioz Stars, 2021-09
    91/100 stars
      Buy from Supplier

    94
    Alomone Labs rabbit anti mglu3 receptor
    Effects of pharmacological <t>mGlu3</t> receptor modulation and Grm3 knock-down on microglial Ccl9 expression in vitro. a , c mRNA expression of Ccl9 under the pro-inflammatory condition (IL-1β + IFNγ) in the presence of LY 379268 (1 μM) + Ro 64-5229 (25 μM) in CTRL and LPD/IL-1β cultured microglia at P4 ( a ) and at P7 ( c ). Data (mean ± SEM) are relative to the gene expression under basal CTRL conditions. Two-way ANOVA followed by the Newman-Keuls multiple comparison; * p
    Rabbit Anti Mglu3 Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mglu3 receptor/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    Image Search Results


    The relative effect of specific α7-nAChR antagonists to block ACh (10 −5 M) elicited responses. A- αBgTx attenuated the ACh evoked contractions in a concentration dependent manner. The blocking effects of 50 nM and 100 nM toxin were statistically significant. The recovery is almost complete. B- The block by 50 nM αA-CTx PIVA was not significant, while the α-CTx ImI at 100 nM blocked almost completely the ACh elicited contraction. The recovery from block was partial. n = number of muscles. Asterisks indicate a significant difference from the control value: *P

    Journal: PLoS ONE

    Article Title: Nicotinic Acetylcholine Receptors Containing the α7-Like Subunit Mediate Contractions of Muscles Responsible for Space Positioning of the Snail, Helix pomatia L. Tentacle

    doi: 10.1371/journal.pone.0109538

    Figure Lengend Snippet: The relative effect of specific α7-nAChR antagonists to block ACh (10 −5 M) elicited responses. A- αBgTx attenuated the ACh evoked contractions in a concentration dependent manner. The blocking effects of 50 nM and 100 nM toxin were statistically significant. The recovery is almost complete. B- The block by 50 nM αA-CTx PIVA was not significant, while the α-CTx ImI at 100 nM blocked almost completely the ACh elicited contraction. The recovery from block was partial. n = number of muscles. Asterisks indicate a significant difference from the control value: *P

    Article Snippet: The α-bungarotoxin (αBgTx), α-conotoxin ImI (α-CTx IMI) and αA-conotoxin PIVA (αA-CTx PIVA) were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Blocking Assay, Concentration Assay

    CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p

    Journal: The Journal of allergy and clinical immunology

    Article Title: Mast Cell CRF2 Suppresses Mast Cell Degranulation and Limits the Severity of Anaphylaxis and Stress-Induced Intestinal Permeability

    doi: 10.1016/j.jaci.2018.08.053

    Figure Lengend Snippet: CRF2−/− BMMCs exhibit heightened intracellular Ca 2+ store release and expression of SOCE channels. BMMCs derived from WT and CRF 2 −/− mice were loaded with Fluo 4, and intracellular Ca 2+ levels were measured following stimulation with IgE/DNP. A,B: Representative intracellular Ca 2+ traces for experiments conducted under Ca 2+ -replete (A) or Ca 2+ -free (1 mM EDTA; B) conditions. C: Mean peak change in fluorescence following IgE/DNP stimulus presented as ∆ peak fluorescence. D-I: Representative Western blots and densitometry analysis for STIM1 (D, G), TRPC1 (E, H), and Orai (F, I) in WT and CRF 2 −/− BMMCs. *Significance between groups was determined by an unpaired two-tailed t-test (C, G-I),*p

    Article Snippet: The membranes were blocked with 5% w/v BSA in Tris-buffered saline (TBS) with 0.1% Tween 20 (TBS-T) for 1 hour at room temperature, washed in TBS-T, incubated with the following Rabbit Anti-Human antibodies: STIM1 (alomone labs #ACC-063;1:500 dilution), Anti-TRPC1 (alomone labs #ACC-010;1:600 dilution), Anti-Orai1 (alomone labs #ACC-060;1:400 dilution), and β-actin (cell signaling, 4970, 1:1000) diluted in 5% w/v BSA in TBS-T for overnight at 4°C or 2 hours at room temperature, and then washed with TBS-T.

    Techniques: Expressing, Derivative Assay, Mouse Assay, Fluorescence, Western Blot, Two Tailed Test

    Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Journal: PLoS ONE

    Article Title: MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis

    doi: 10.1371/journal.pone.0064551

    Figure Lengend Snippet: Assessing MARCKS function on acrosomal exocytosis in non-permeabilized sperm. ( A ) Capacitated and non-permeabilized sperm were treated for 30 minutes at 37°C with increasing concentrations of a permeable tetramethylrhodamine-labeled MARCKS ED peptide (ED-TMR). Acrosomal exocytosis was then initiated by adding 10 µM calcium ionophore A23187 (circles), 200 nM PMA (squares) or 15 µM progesterone (triangles), and the incubation continued for 15 minutes. The percentage of reacted sperm was normalized as described in Materials and Methods . The data represent the means±SEM of at least three independent experiments. The asterisks indicate significant differences from similar conditions without ED-TMR (*, p

    Article Snippet: When data were normalized against MARCKS, similar results were obtained (see for the effect of A23187; the results for PMA and progesterone are not shown).

    Techniques: Labeling, Incubation

    Effects of pharmacological mGlu3 receptor modulation and Grm3 knock-down on microglial Ccl9 expression in vitro. a , c mRNA expression of Ccl9 under the pro-inflammatory condition (IL-1β + IFNγ) in the presence of LY 379268 (1 μM) + Ro 64-5229 (25 μM) in CTRL and LPD/IL-1β cultured microglia at P4 ( a ) and at P7 ( c ). Data (mean ± SEM) are relative to the gene expression under basal CTRL conditions. Two-way ANOVA followed by the Newman-Keuls multiple comparison; * p

    Journal: Journal of Neuroinflammation

    Article Title: mGlu3 receptor regulates microglial cell reactivity in neonatal rats

    doi: 10.1186/s12974-020-02049-z

    Figure Lengend Snippet: Effects of pharmacological mGlu3 receptor modulation and Grm3 knock-down on microglial Ccl9 expression in vitro. a , c mRNA expression of Ccl9 under the pro-inflammatory condition (IL-1β + IFNγ) in the presence of LY 379268 (1 μM) + Ro 64-5229 (25 μM) in CTRL and LPD/IL-1β cultured microglia at P4 ( a ) and at P7 ( c ). Data (mean ± SEM) are relative to the gene expression under basal CTRL conditions. Two-way ANOVA followed by the Newman-Keuls multiple comparison; * p

    Article Snippet: Blots were then incubated overnight with rabbit anti-mGlu3 receptor (1:600; AGC-012, Alomone Labs, Jerusalem, Israël) in blocking solution at 4 °C.

    Techniques: Expressing, In Vitro, Cell Culture

    Developmental expression of mGlu3 receptors following the LPD/IL-1β double hit in rat. a , b Grm3 mRNA expression in cortex ( a ) and in microglia ( b ) sorted from CTRL and LPD/IL-1β animals. Data (mean ± SEM) are relative to Grm3 expression in sorted cells of CTRL cortex and CTRL microglia at PND1. Two-way ANOVA followed by the Newman-Keuls multiple comparison test; ** p

    Journal: Journal of Neuroinflammation

    Article Title: mGlu3 receptor regulates microglial cell reactivity in neonatal rats

    doi: 10.1186/s12974-020-02049-z

    Figure Lengend Snippet: Developmental expression of mGlu3 receptors following the LPD/IL-1β double hit in rat. a , b Grm3 mRNA expression in cortex ( a ) and in microglia ( b ) sorted from CTRL and LPD/IL-1β animals. Data (mean ± SEM) are relative to Grm3 expression in sorted cells of CTRL cortex and CTRL microglia at PND1. Two-way ANOVA followed by the Newman-Keuls multiple comparison test; ** p

    Article Snippet: Blots were then incubated overnight with rabbit anti-mGlu3 receptor (1:600; AGC-012, Alomone Labs, Jerusalem, Israël) in blocking solution at 4 °C.

    Techniques: Expressing

    Pharmacological mGlu3 receptor blockade and microglial reactivity in response to inflammatory stimulation in vitro. a , b Microglial cells sorted from P4 pups were stained with Iba1 (green) and DAPI (blue) under basal conditions ± LY 2389575 (5 μM). Representative photomicrographs at × 40 magnification are shown in a (scale bar = 50 μm). The cell area, cell perimeter, and cell circularity were assessed in b (mean cell number, 162 ± 10). Data (mean ± SEM). Unpaired t test; ** p

    Journal: Journal of Neuroinflammation

    Article Title: mGlu3 receptor regulates microglial cell reactivity in neonatal rats

    doi: 10.1186/s12974-020-02049-z

    Figure Lengend Snippet: Pharmacological mGlu3 receptor blockade and microglial reactivity in response to inflammatory stimulation in vitro. a , b Microglial cells sorted from P4 pups were stained with Iba1 (green) and DAPI (blue) under basal conditions ± LY 2389575 (5 μM). Representative photomicrographs at × 40 magnification are shown in a (scale bar = 50 μm). The cell area, cell perimeter, and cell circularity were assessed in b (mean cell number, 162 ± 10). Data (mean ± SEM). Unpaired t test; ** p

    Article Snippet: Blots were then incubated overnight with rabbit anti-mGlu3 receptor (1:600; AGC-012, Alomone Labs, Jerusalem, Israël) in blocking solution at 4 °C.

    Techniques: In Vitro, Staining

    mGlu3 receptor activation and microglial reactivity in CTRL and LPD/IL-1β cultured microglia at P4. a , b Microglial cells were stained with Iba1 (green) and DAPI (blue) under basal and challenged conditions (IL-1β + IFNγ) ± LY 379268 (1 μM) + Ro 64-5229 (25 μM). Representative photomicrographs at × 40 magnification are shown in a (scale bar = 50 μm). Four cell-culture wells for each condition were analyzed (mean cell number, 165 ± 1 7) and the cell area, cell perimeter, and cell circularity were assessed in b . Data (mean ± SEM). Two-way ANOVA followed by the Newman-Keuls multiple comparison test; * p

    Journal: Journal of Neuroinflammation

    Article Title: mGlu3 receptor regulates microglial cell reactivity in neonatal rats

    doi: 10.1186/s12974-020-02049-z

    Figure Lengend Snippet: mGlu3 receptor activation and microglial reactivity in CTRL and LPD/IL-1β cultured microglia at P4. a , b Microglial cells were stained with Iba1 (green) and DAPI (blue) under basal and challenged conditions (IL-1β + IFNγ) ± LY 379268 (1 μM) + Ro 64-5229 (25 μM). Representative photomicrographs at × 40 magnification are shown in a (scale bar = 50 μm). Four cell-culture wells for each condition were analyzed (mean cell number, 165 ± 1 7) and the cell area, cell perimeter, and cell circularity were assessed in b . Data (mean ± SEM). Two-way ANOVA followed by the Newman-Keuls multiple comparison test; * p

    Article Snippet: Blots were then incubated overnight with rabbit anti-mGlu3 receptor (1:600; AGC-012, Alomone Labs, Jerusalem, Israël) in blocking solution at 4 °C.

    Techniques: Activation Assay, Cell Culture, Staining