azd 1332  (Alomone Labs)


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    Structured Review

    Alomone Labs azd 1332
    The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or <t>AZD-1332</t> (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.
    Azd 1332, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azd 1332/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azd 1332 - by Bioz Stars, 2023-09
    93/100 stars

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    1) Product Images from "Therapeutic potential of a TrkB agonistic antibody for Alzheimer's disease"

    Article Title: Therapeutic potential of a TrkB agonistic antibody for Alzheimer's disease

    Journal: Theranostics

    doi: 10.7150/thno.44165

    The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or AZD-1332 (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.
    Figure Legend Snippet: The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or AZD-1332 (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay, Cell Culture, Incubation, Western Blot, Activation Assay

    Cell survival function of AS86. ( A ) Attenuation of serum-deprivation induced cell death by AS86. Different doses of BDNF or AS86 were applied to serum-deprived cultures of hTrkB-PC12 cells in the absence or presence of Trk inhibitors (300 nM K252a or 50 nM AZD-1332) for 16 hours, and the levels of apoptosis were determined by the ratio of the number of caspase 3 positive cells to total number of cells, using a caspase 3-substrate kit. Survival rates were measured by the decreased apoptotic levels normalized to that of vehicle treatment (n = 3). ( B ) Attenuation of Aβ induced cell death by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (5 µM). Cell viability was analyzed with ATP level quantification assay 48 hours later (N = 2 independent experiments, n = 6 samples). ( C, D ) Inhibition of Aβ induced apoptotic signals by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (15 µM) for 24 hours. Total and cleaved caspase3 levels were measured using Western blotting (N = 3 independent experiments, n = 3 replicates). Representative Western blots (D) and quantitative plots (C) are presented.
    Figure Legend Snippet: Cell survival function of AS86. ( A ) Attenuation of serum-deprivation induced cell death by AS86. Different doses of BDNF or AS86 were applied to serum-deprived cultures of hTrkB-PC12 cells in the absence or presence of Trk inhibitors (300 nM K252a or 50 nM AZD-1332) for 16 hours, and the levels of apoptosis were determined by the ratio of the number of caspase 3 positive cells to total number of cells, using a caspase 3-substrate kit. Survival rates were measured by the decreased apoptotic levels normalized to that of vehicle treatment (n = 3). ( B ) Attenuation of Aβ induced cell death by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (5 µM). Cell viability was analyzed with ATP level quantification assay 48 hours later (N = 2 independent experiments, n = 6 samples). ( C, D ) Inhibition of Aβ induced apoptotic signals by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (15 µM) for 24 hours. Total and cleaved caspase3 levels were measured using Western blotting (N = 3 independent experiments, n = 3 replicates). Representative Western blots (D) and quantitative plots (C) are presented.

    Techniques Used: Inhibition, Western Blot

    amino acid residues 416 495  (Alomone Labs)


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    Alomone Labs amino acid residues 416 495
    Amino Acid Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    gst fusion protein amino acid 416 495  (Alomone Labs)


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    Structured Review

    Alomone Labs gst fusion protein amino acid 416 495
    Primary antibodies used.
    Gst Fusion Protein Amino Acid 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gst fusion protein amino acid 416 495/product/Alomone Labs
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    1) Product Images from "Expression and Localization of Kv1.1 and Kv3.1b Potassium Channels in the Cochlear Nucleus and Inferior Colliculus after Long-Term Auditory Deafferentation"

    Article Title: Expression and Localization of Kv1.1 and Kv3.1b Potassium Channels in the Cochlear Nucleus and Inferior Colliculus after Long-Term Auditory Deafferentation

    Journal: Brain Sciences

    doi: 10.3390/brainsci10010035


    Figure Legend Snippet: Primary antibodies used.

    Techniques Used: Recombinant

    residues 416 495  (Alomone Labs)


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    Alomone Labs residues 416 495
    Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    amino acid residues 416 495  (Alomone Labs)


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    Alomone Labs amino acid residues 416 495
    Amino Acid Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    residues 416 495  (Alomone Labs)


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    Alomone Labs residues 416 495
    Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit against residues 416 495  (Alomone Labs)


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    Alomone Labs rabbit against residues 416 495
    Rabbit Against Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    amino acid residues 416 495  (Alomone Labs)


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    Alomone Labs amino acid residues 416 495
    Amino Acid Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    amino acid residues 416 495  (Alomone Labs)


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    Alomone Labs amino acid residues 416 495
    Amino Acid Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs azd 1332
    The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or <t>AZD-1332</t> (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.
    Azd 1332, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs amino acid residues 416 495
    The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or <t>AZD-1332</t> (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.
    Amino Acid Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amino acid residues 416 495/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amino acid residues 416 495 - by Bioz Stars, 2023-09
    86/100 stars
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    86
    Alomone Labs gst fusion protein amino acid 416 495
    Primary antibodies used.
    Gst Fusion Protein Amino Acid 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gst fusion protein amino acid 416 495/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    gst fusion protein amino acid 416 495 - by Bioz Stars, 2023-09
    86/100 stars
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    86
    Alomone Labs residues 416 495
    Primary antibodies used.
    Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/residues 416 495/product/Alomone Labs
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    residues 416 495 - by Bioz Stars, 2023-09
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    86
    Alomone Labs rabbit against residues 416 495
    Primary antibodies used.
    Rabbit Against Residues 416 495, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or AZD-1332 (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.

    Journal: Theranostics

    Article Title: Therapeutic potential of a TrkB agonistic antibody for Alzheimer's disease

    doi: 10.7150/thno.44165

    Figure Lengend Snippet: The specificity of TrkB agonistic antibody AS86. (A) AS86 at different concentrations (0.1 nM, 1 nM and 10 nM) was added to the plates coated with different proteins (0.1 μg TrkA, TrkB, TrkC, or p75 respectively), and ELISA was used to examine the binding capacity of AS86. (B, C) Cultured hippocampal neurons (DIV10) were pretreated with the Trk inhibitors k252a (300 nM) or AZD-1332 (100 nM) for 60 min before incubation with mIgG (3 nM), BDNF (1 nM), or AS86 (3 nM) for 15 min (N = 2, n = 3). The Western blots of TrkB Y515 and Y816 sites activation (B) and the quantitative plots (C) are presented. Unless specifically indicated otherwise, statistical analyses in this and all other figures were carried out using one-way ANOVA followed by post hoc test. Symbols for P values (for both ANOVA and Student's t -test): *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: P < 0.0001. The quantification data in this and all other figures were presented as mean ± SEM.

    Article Snippet: The reagents included BDNF protein (Sino Biological Inc, 50240-MNAS), Mouse IgG (YEASEN, 3611ES10), and β-Amyloid protein (25-35) (Synpeptide), K252a (Bio Vision, 2013-500), AZD-1332 (Alomeone labs, A-495).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Cell Culture, Incubation, Western Blot, Activation Assay

    Cell survival function of AS86. ( A ) Attenuation of serum-deprivation induced cell death by AS86. Different doses of BDNF or AS86 were applied to serum-deprived cultures of hTrkB-PC12 cells in the absence or presence of Trk inhibitors (300 nM K252a or 50 nM AZD-1332) for 16 hours, and the levels of apoptosis were determined by the ratio of the number of caspase 3 positive cells to total number of cells, using a caspase 3-substrate kit. Survival rates were measured by the decreased apoptotic levels normalized to that of vehicle treatment (n = 3). ( B ) Attenuation of Aβ induced cell death by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (5 µM). Cell viability was analyzed with ATP level quantification assay 48 hours later (N = 2 independent experiments, n = 6 samples). ( C, D ) Inhibition of Aβ induced apoptotic signals by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (15 µM) for 24 hours. Total and cleaved caspase3 levels were measured using Western blotting (N = 3 independent experiments, n = 3 replicates). Representative Western blots (D) and quantitative plots (C) are presented.

    Journal: Theranostics

    Article Title: Therapeutic potential of a TrkB agonistic antibody for Alzheimer's disease

    doi: 10.7150/thno.44165

    Figure Lengend Snippet: Cell survival function of AS86. ( A ) Attenuation of serum-deprivation induced cell death by AS86. Different doses of BDNF or AS86 were applied to serum-deprived cultures of hTrkB-PC12 cells in the absence or presence of Trk inhibitors (300 nM K252a or 50 nM AZD-1332) for 16 hours, and the levels of apoptosis were determined by the ratio of the number of caspase 3 positive cells to total number of cells, using a caspase 3-substrate kit. Survival rates were measured by the decreased apoptotic levels normalized to that of vehicle treatment (n = 3). ( B ) Attenuation of Aβ induced cell death by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (5 µM). Cell viability was analyzed with ATP level quantification assay 48 hours later (N = 2 independent experiments, n = 6 samples). ( C, D ) Inhibition of Aβ induced apoptotic signals by AS86. Hippocampal neurons were pretreated with AS86 or BDNF for 30 minutes, followed by treatment with Aβ (25-35) (15 µM) for 24 hours. Total and cleaved caspase3 levels were measured using Western blotting (N = 3 independent experiments, n = 3 replicates). Representative Western blots (D) and quantitative plots (C) are presented.

    Article Snippet: The reagents included BDNF protein (Sino Biological Inc, 50240-MNAS), Mouse IgG (YEASEN, 3611ES10), and β-Amyloid protein (25-35) (Synpeptide), K252a (Bio Vision, 2013-500), AZD-1332 (Alomeone labs, A-495).

    Techniques: Inhibition, Western Blot

    Journal: Brain Sciences

    Article Title: Expression and Localization of Kv1.1 and Kv3.1b Potassium Channels in the Cochlear Nucleus and Inferior Colliculus after Long-Term Auditory Deafferentation

    doi: 10.3390/brainsci10010035

    Figure Lengend Snippet: Primary antibodies used.

    Article Snippet: Kv1.1 , GST fusion protein amino acid 416–495 (Intracellular C-terminus) of mouse Kv1.1 , Polyclonal rabbit, APC009, Alomone (RRID: AB_2040144) , IHC 1:500 WB 1:300.

    Techniques: Recombinant