P2x7r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "P2X7 Receptor Mediates Spinal Microglia Activation of Visceral Hyperalgesia in a Rat Model of Chronic Pancreatitis"
Article Title: P2X7 Receptor Mediates Spinal Microglia Activation of Visceral Hyperalgesia in a Rat Model of Chronic Pancreatitis
Journal: Cellular and Molecular Gastroenterology and Hepatology
Figure Legend Snippet: The abdominal reflex upon von Frey filament (VFF) stimuli in baseline showed no significant difference among the four groups (mixed-design two-way analysis of variance) in ( A ) brilliant blue G (BBG) treatment experiments ( P = .792); ( B ) BBG prevention experiments ( P = .401); and ( C ) P2X7R small-interfering RNA (siRNA) treatment experiments ( P = .247) .
Techniques Used: Small Interfering RNA
Figure Legend Snippet: Brilliant blue G (BBG) treatment attenuated P2X7R expression in the spinal cord. ( A ) The expression level of P2X7R in the spinal cord was significantly increased in the trinitrobenzene sulfonic acid (TNBS)-treated rats (T + V) compared with the control rats (V + V). P2X7R expression was alleviated by intrathecal (IT) BBG treatment (T + B) ( P = .005, one-way analysis of variance [ANOVA]). * P
Techniques Used: Expressing
Figure Legend Snippet: Brilliant blue G (BBG) administration prevents visceral hyperalgesia via the inhibition of P2X7R. ( A ) The mean abdominal reflexes in response to von Frey filament (VFF) stimulation in trinitrobenzene sulfonic acid (TNBS)-treated rats (V + T) were significantly higher than in the control rats (V + V). This effect was prevented by intrathecal (IT) BBG pretreatment (B + T) ( P
Techniques Used: Inhibition
Figure Legend Snippet: P2X7R expression with OX-42-positive cells in dorsal horn of the spinal cord. In trinitrobenzene sulfonic acid (TNBS)-treated rats, P2X7R ( red ) staining colocalized with OX-42-positive cells (green) ( A, B ) but not with NeuN-positive cells ( green ) ( C, D ) or GFAP-positive cells (green) ( E, F ). Double immunofluorescence labeling for P2X7R and for cell-type specific markers: NeuN for neuron, GFAP for astrocyte, and OX42 for microglia. DAPI (4′,6-diamidino-2-phenylindole) staining identifies all cell nuclei ( blue ). Scale bar: 50 μm ( A, C, E ); 20 μm ( B, D, F ). The arrows indicate the colocalization of P2X7R expression with the microglia marker.
Techniques Used: Expressing, Staining, Immunofluorescence, Labeling, Marker
Figure Legend Snippet: P2X7R small-interfering RNA (siRNA) treatment experiment. ( A , B , D ) Within-group analysis in the brilliant blue G (BBG) treatment experiments showed the abdominal reflexes upon von Frey filament (VFF) stimuli were similar before and after P2X7R siRNA/nontargeted siRNA treatment. ( C ) In the trinitrobenzene sulfonic acid (TNBS)-induced chronic pancreatitis (CP) rats, the abdominal reflexes upon VFF stimuli were significantly increased after nontargeted siRNA treatment. Analysis by two-way repeated measurement analysis of variance. ( A ) P = .391; ( B ) P = .069; ( C ) * P = .007; ( D ) P = .222.
Techniques Used: Small Interfering RNA
Figure Legend Snippet: Pancreatic histology in chronic pancreatitis (CP) rats treated with vehicle ( A ), brilliant blue G (BBG) ( B ), and P2X7R small-interfering RNA (siRNA) ( C ). All groups of rats showed markedly inflammatory cells infiltration, large regions of acinar loss and periductular and intralobular fibrosis, and lost acinar tissue replaced with tubular structures. No significant morphologic changes could be observed after either BBG or siRNA treatment in CP rats. H E stain; scale bar: 100 μm.
Techniques Used: Small Interfering RNA, Staining
Figure Legend Snippet: Increased P2X7R expression levels in the spinal cord after chronic pancreatitis (CP). ( A ) Immunohistochemistry of the spinal cord dorsal horn in vehicle-treated rats. ( B ) Three weeks after induction of CP, P2X7R expression was increased. ( C ) Representative Western blots of P2X7R expression in the thoracic spinal cord. The P2X7R expression level was significantly increased after CP. ( D ) The P2X7R mRNA expression level of was also significantly increased after CP. Scale bar = 100 μm in A and B . * P
Techniques Used: Expressing, Immunohistochemistry, Western Blot
Figure Legend Snippet: Intrathecal (IT) administration of P2X7R-targeted small-interfering RNA (siRNA) ameliorated the chronic pancreatitis (CP)-related nocifensive behaviors. ( A ) The abdominal reflex in response to von Frey filament (VFF) stimulation in rats after intraductal infusion of TNBS and nontargeted siRNA treatment (T + N) was significantly increased compared with the control rats (V + N). This hyperalgesia was ameliorated by IT injection of P2X7R siRNA (T + KD) ( P
Techniques Used: Small Interfering RNA, Injection