radicicol  (Alomone Labs)


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    Structured Review

    Alomone Labs radicicol
    Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with <t>radicicol</t> (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx
    Radicicol, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/radicicol/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    radicicol - by Bioz Stars, 2022-07
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    1) Product Images from "Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression"

    Article Title: Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0809669106

    Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx
    Figure Legend Snippet: Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx

    Techniques Used: Inhibition

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    Alomone Labs radicicol
    HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), <t>radicicol,</t> FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3
    Radicicol, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 1 article reviews
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    Alomone Labs isopimaric acid
    ( a ) Time course of the inhibitory effect of simvastatin (10 μ M ) on the BK Ca amplitude. Calibration bars: 1000 pA and 100 ms. ( b ) Time course of the effect of <t>isopimaric</t> acid (10 μ M ), with and without simvastatin
    Isopimaric Acid, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), radicicol, FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3

    Journal: Epigenetics & Chromatin

    Article Title: Pho dynamically interacts with Spt5 to facilitate transcriptional switches at the hsp70 locus

    doi: 10.1186/s13072-017-0166-9

    Figure Lengend Snippet: HSF recruitment to chromatin is necessary and sufficient for the dynamic protein–protein interaction between Pho and Spt5. a ChIP-qPCR measurements for the occupancy levels of HSF at the hsp70 locus in S2 DRSC cells upon HS (heat shock), radicicol, FP + HS (flavopiridol + heat shock) and Na Sal (Sodium Salicylate) treatment conditions, respectively. In case of radicicol treatment, the control condition was DMSO treatment. For FP + HS, the control condition was FP control and for Na Sal, the control condition was nuclease-free water. Distance of the location of the primers used from the hsp70 TSS is − 85 bp and has been depicted in the form of a cartoon below the data figure. b qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HS, radicicol, FP + HS and Na Sal treatment conditions, respectively. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. c co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). The S2 DRSC cells were subjected to HS, radicicol, FP + HS and Na Sal, respectively; cell lysates were used for pull-downs with an anti-FLAG antibody and later probed by Western blot with an anti-HA antibody. d Quantification of the co-IP assays in c . Details of the procedure used for quantification can be found in Materials and Methods. e co-IP assays of S2 DRSC cells transiently transfected with plasmids expressing FLAG-tagged Spt5 (FLAG-Spt5) and HA-tagged Pho (HA-Pho). Cells were either treated with HSF or LacZ RNAi for 4 days, then either maintained at control conditions or heat shocked, cell lysates were prepared and used for pull-downs using an anti-FLAG antibody and later probed by Western blot using an anti-HA antibody. f qRT-PCR measurements of hsp70 transcript levels in S2 DRSC cells under HSF and LacZ RNAi conditions. Distance of the location of the primers used from the hsp70 TSS is + 1492 bp and has been depicted in the form of a cartoon below the data figure. Data information: In a and b , data are presented as mean ± SEM for n = 2, while in e , data are presented as mean ± SEM for n = 3

    Article Snippet: Chemicals and antibodiesThe following chemicals were used in this study: flavopiridol (F3055, Sigma-Aldrich), radicicol (R-370, Alomone), sodium salicylate (S3007, Sigma-Aldrich), copper sulphate (102790, Merck).

    Techniques: Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Co-Immunoprecipitation Assay, Transfection, Expressing, Western Blot

    Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression

    doi: 10.1073/pnas.0809669106

    Figure Lengend Snippet: Hsp90 is required for the stability of the Trx protein. ( A ) In Drosophila Kc cells, inhibition of Hsp90 with radicicol (Radic) resulted in depletion of Trx protein. After 4 h of Radic treatment with 20 μM (lane 2) and 40 μM (lane 4), Trx

    Article Snippet: A total of 3 × 106 /ml cells per well in a 6-well plate were seeded 1 day before drug treatment and treated with different concentrations (3, 5, 10, 20, 40, and 80 μM) of radicicol dissolved in DMSO (Alomone labs) ( A and data not shown) for 4 h. The Kc cells treated with equivalent amounts of DMSO vehicle were used as mock-treated cells.

    Techniques: Inhibition

    ( a ) Time course of the inhibitory effect of simvastatin (10 μ M ) on the BK Ca amplitude. Calibration bars: 1000 pA and 100 ms. ( b ) Time course of the effect of isopimaric acid (10 μ M ), with and without simvastatin

    Journal:

    Article Title: Modulation by simvastatin of iberiotoxin-sensitive, Ca2+-activated K+ channels of porcine coronary artery smooth muscle cells

    doi: 10.1038/sj.bjp.0707327

    Figure Lengend Snippet: ( a ) Time course of the inhibitory effect of simvastatin (10 μ M ) on the BK Ca amplitude. Calibration bars: 1000 pA and 100 ms. ( b ) Time course of the effect of isopimaric acid (10 μ M ), with and without simvastatin

    Article Snippet: Iberiotoxin and isopimaric acid were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques:

    Effect of ( a ) NS 1619 (10 μ M ), ( b ) isopimaric acid (10 μ M ) and ( c ) iberiotoxin (100 n M ) on the whole-cell, outward K + currents of pig coronary artery smooth muscle cells. Representative whole-cell, outward

    Journal:

    Article Title: Modulation by simvastatin of iberiotoxin-sensitive, Ca2+-activated K+ channels of porcine coronary artery smooth muscle cells

    doi: 10.1038/sj.bjp.0707327

    Figure Lengend Snippet: Effect of ( a ) NS 1619 (10 μ M ), ( b ) isopimaric acid (10 μ M ) and ( c ) iberiotoxin (100 n M ) on the whole-cell, outward K + currents of pig coronary artery smooth muscle cells. Representative whole-cell, outward

    Article Snippet: Iberiotoxin and isopimaric acid were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: