arcaine sulfate  (Alomone Labs)


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    Structured Review

    Alomone Labs arcaine sulfate
    LBD mutations do not affect spermine binding site. ( a ) Representative traces from oocytes co-expressing hGluN1a and hGluN2B- wt (blue) or GluN2B-G689C (cyan) or G689S (red) in response to block by <t>arcaine</t> (200 μM), after receptors have been activated by saturating glycine (100 μM) and glutamate (5 mM) at pH 6.5. Then, application of incrementing concentrations of spermine (concentration indicated next to plot; in μM) are added in the presence of arcaine, showing increases of the current despite antagonist. Arrowhead indicates I basal at pH 6.5, prior block by arcaine. ( b ) Summary of arcaine-dependent antagonism of the current. ( c ) Summary of spermine potentiation at pH=6.5 in the presence of 200 μM arcaine. ( d ) Summary of I basal at pH=6.5, showing that 2B wt receptors exhibit smallest I basal . *, p
    Arcaine Sulfate, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arcaine sulfate/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    arcaine sulfate - by Bioz Stars, 2022-05
    93/100 stars

    Images

    1) Product Images from "Two de novo GluN2B mutations affect multiple NMDAR-functions and instigate severe pediatric encephalopathy"

    Article Title: Two de novo GluN2B mutations affect multiple NMDAR-functions and instigate severe pediatric encephalopathy

    Journal: eLife

    doi: 10.7554/eLife.67555

    LBD mutations do not affect spermine binding site. ( a ) Representative traces from oocytes co-expressing hGluN1a and hGluN2B- wt (blue) or GluN2B-G689C (cyan) or G689S (red) in response to block by arcaine (200 μM), after receptors have been activated by saturating glycine (100 μM) and glutamate (5 mM) at pH 6.5. Then, application of incrementing concentrations of spermine (concentration indicated next to plot; in μM) are added in the presence of arcaine, showing increases of the current despite antagonist. Arrowhead indicates I basal at pH 6.5, prior block by arcaine. ( b ) Summary of arcaine-dependent antagonism of the current. ( c ) Summary of spermine potentiation at pH=6.5 in the presence of 200 μM arcaine. ( d ) Summary of I basal at pH=6.5, showing that 2B wt receptors exhibit smallest I basal . *, p
    Figure Legend Snippet: LBD mutations do not affect spermine binding site. ( a ) Representative traces from oocytes co-expressing hGluN1a and hGluN2B- wt (blue) or GluN2B-G689C (cyan) or G689S (red) in response to block by arcaine (200 μM), after receptors have been activated by saturating glycine (100 μM) and glutamate (5 mM) at pH 6.5. Then, application of incrementing concentrations of spermine (concentration indicated next to plot; in μM) are added in the presence of arcaine, showing increases of the current despite antagonist. Arrowhead indicates I basal at pH 6.5, prior block by arcaine. ( b ) Summary of arcaine-dependent antagonism of the current. ( c ) Summary of spermine potentiation at pH=6.5 in the presence of 200 μM arcaine. ( d ) Summary of I basal at pH=6.5, showing that 2B wt receptors exhibit smallest I basal . *, p

    Techniques Used: Binding Assay, Expressing, Blocking Assay, Concentration Assay

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    • cleavage resistant prongf  (Alomone Labs)
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    Alomone Labs cleavage resistant prongf
    <t>Overexpression</t> of <t>proNGF</t> induced glial Müller activation and inflammation. Immunohistochemical analysis of retinas was performed 4 weeks after intravitreal injections of pGFP alone (control) ( a–e ), proNGF plus scrambled p75 NTR shRNA (proNGF+Scr)
    Cleavage Resistant Prongf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleavage resistant prongf/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cleavage resistant prongf - by Bioz Stars, 2022-05
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    Overexpression of proNGF induced glial Müller activation and inflammation. Immunohistochemical analysis of retinas was performed 4 weeks after intravitreal injections of pGFP alone (control) ( a–e ), proNGF plus scrambled p75 NTR shRNA (proNGF+Scr)

    Journal: Diabetologia

    Article Title: Modulation of p75NTR prevents diabetes- and proNGF-induced retinal inflammation and blood–retina barrier breakdown in mice and rats

    doi: 10.1007/s00125-013-2998-6

    Figure Lengend Snippet: Overexpression of proNGF induced glial Müller activation and inflammation. Immunohistochemical analysis of retinas was performed 4 weeks after intravitreal injections of pGFP alone (control) ( a–e ), proNGF plus scrambled p75 NTR shRNA (proNGF+Scr)

    Article Snippet: Representative blots ( ) and statistical analysis show that overexpression of cleavage-resistant proNGF plus scrambled shRNA induced a 2.5-fold increase in proNGF , a 1.8-fold increase in p75 , a 3.6-fold increase in NFκB , a 1.7-fold increase in TNF-α ( ) and a 2.3-fold increase in IL-1β ( ).

    Techniques: Over Expression, Activation Assay, Immunohistochemistry, shRNA

    ProNGF activates JNK and NF-κB in primary VS cultures. A . Western blots of protein lysates from primary VS cultures treated Ad5.empty vector (Ad.EV) or Ad5.merlin (Ad.merlin) and probed with anti-phosphorylated JNK antibodies. The blots were stripped

    Journal: Glia

    Article Title: p75NTR is highly expressed in vestibular schwannomas and promotes cell survival by activating NF-κB

    doi: 10.1002/glia.22709

    Figure Lengend Snippet: ProNGF activates JNK and NF-κB in primary VS cultures. A . Western blots of protein lysates from primary VS cultures treated Ad5.empty vector (Ad.EV) or Ad5.merlin (Ad.merlin) and probed with anti-phosphorylated JNK antibodies. The blots were stripped

    Article Snippet: Primary human VS cultures and mouse sciatic nerve Schwann cells maintained in the presence or absence I-JIP or SP600125 (JNK inhibitors) were treated with cleavage resistant proNGF (Alomone Labs, Jerusalem, Israel).

    Techniques: Western Blot, Plasmid Preparation

    ProNGF protects VS cells from apoptosis due to JNK inhibition. A-D . Primary VS cultures were maintained in the presence of the JNK inhibitors, I-JIP or SP600125, with or without treatment with proNGF (3 nM) (A) of proBDNF (3 nM) (D). Cultures were immunostained

    Journal: Glia

    Article Title: p75NTR is highly expressed in vestibular schwannomas and promotes cell survival by activating NF-κB

    doi: 10.1002/glia.22709

    Figure Lengend Snippet: ProNGF protects VS cells from apoptosis due to JNK inhibition. A-D . Primary VS cultures were maintained in the presence of the JNK inhibitors, I-JIP or SP600125, with or without treatment with proNGF (3 nM) (A) of proBDNF (3 nM) (D). Cultures were immunostained

    Article Snippet: Primary human VS cultures and mouse sciatic nerve Schwann cells maintained in the presence or absence I-JIP or SP600125 (JNK inhibitors) were treated with cleavage resistant proNGF (Alomone Labs, Jerusalem, Israel).

    Techniques: Inhibition

    ProNGF fails to induce apoptosis in primary vestibular schwannoma cultures. A . Primary VS cultures immunostained with anti-p75 NTR antibody (green). Nuclei are labeled with DAPI (blue). B,C . Primary human VS cultures were treated with escalating doses

    Journal: Glia

    Article Title: p75NTR is highly expressed in vestibular schwannomas and promotes cell survival by activating NF-κB

    doi: 10.1002/glia.22709

    Figure Lengend Snippet: ProNGF fails to induce apoptosis in primary vestibular schwannoma cultures. A . Primary VS cultures immunostained with anti-p75 NTR antibody (green). Nuclei are labeled with DAPI (blue). B,C . Primary human VS cultures were treated with escalating doses

    Article Snippet: To determine whether VS cells are likewise susceptible to proNGF-mediated apoptosis, we treated primary VS cultures with escalating doses of cleavage-resistant proNGF.

    Techniques: Labeling