nt3 (Alomone Labs)


Structured Review

Nt3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nt3/product/Alomone Labs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs"
Article Title: Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs
Journal: The Journal of Cell Biology
doi: 10.1083/jcb.200703209

Figure Legend Snippet: Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.
Techniques Used: Real-time Polymerase Chain Reaction
2) Product Images from "Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs"
Article Title: Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs
Journal: The Journal of Cell Biology
doi: 10.1083/jcb.200703209

Figure Legend Snippet: Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.
Techniques Used: Real-time Polymerase Chain Reaction
3) Product Images from "Degeneration in the Ventral Cochlear Nucleus after Severe Noise Damage in Mice"
Article Title: Degeneration in the Ventral Cochlear Nucleus after Severe Noise Damage in Mice
Journal: Journal of Neuroscience Research
doi: 10.1002/jnr.22793

Figure Legend Snippet: Landscape presentation of immunofluorescent double labeling in PVCN-A of control and noise exposed mice at 1, 2, 4, and 8 weeks. UPPER PANEL: NT3 (green) with SV2 (red); yellow is co-localization. By the eighth week the decrease in green and yellow suggests
Techniques Used: Labeling, Mouse Assay

Figure Legend Snippet: Immunofluorescent imaging of SV2, NT3 and GLT1 in PVCN-A of control and noise exposed mice at 1, 2, 4, and 8 weeks. By 8 weeks SV2 and NT3 reached the lowest level, as degeneration attained its peak. For other changes see Results. Scale: 20 μm.
Techniques Used: Imaging, Mouse Assay