nt3  (Alomone Labs)


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    Alomone Labs nt3
    Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or <t>NT3</t> treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.
    Nt3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nt3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nt3 - by Bioz Stars, 2022-05
    94/100 stars

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    1) Product Images from "Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs"

    Article Title: Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.200703209

    Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.
    Figure Legend Snippet: Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.

    Techniques Used: Real-time Polymerase Chain Reaction

    2) Product Images from "Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs"

    Article Title: Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.200703209

    Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.
    Figure Legend Snippet: Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.

    Techniques Used: Real-time Polymerase Chain Reaction

    3) Product Images from "Degeneration in the Ventral Cochlear Nucleus after Severe Noise Damage in Mice"

    Article Title: Degeneration in the Ventral Cochlear Nucleus after Severe Noise Damage in Mice

    Journal: Journal of Neuroscience Research

    doi: 10.1002/jnr.22793

    Landscape presentation of immunofluorescent double labeling in PVCN-A of control and noise exposed mice at 1, 2, 4, and 8 weeks. UPPER PANEL: NT3 (green) with SV2 (red); yellow is co-localization. By the eighth week the decrease in green and yellow suggests
    Figure Legend Snippet: Landscape presentation of immunofluorescent double labeling in PVCN-A of control and noise exposed mice at 1, 2, 4, and 8 weeks. UPPER PANEL: NT3 (green) with SV2 (red); yellow is co-localization. By the eighth week the decrease in green and yellow suggests

    Techniques Used: Labeling, Mouse Assay

    Immunofluorescent imaging of SV2, NT3 and GLT1 in PVCN-A of control and noise exposed mice at 1, 2, 4, and 8 weeks. By 8 weeks SV2 and NT3 reached the lowest level, as degeneration attained its peak. For other changes see Results. Scale: 20 μm.
    Figure Legend Snippet: Immunofluorescent imaging of SV2, NT3 and GLT1 in PVCN-A of control and noise exposed mice at 1, 2, 4, and 8 weeks. By 8 weeks SV2 and NT3 reached the lowest level, as degeneration attained its peak. For other changes see Results. Scale: 20 μm.

    Techniques Used: Imaging, Mouse Assay

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    Alomone Labs nt3
    Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or <t>NT3</t> treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.
    Nt3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nt3/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    nt3 - by Bioz Stars, 2022-05
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    Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.

    Journal: The Journal of Cell Biology

    Article Title: Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs

    doi: 10.1083/jcb.200703209

    Figure Lengend Snippet: Axonal stimulation with growth-promoting and growth-inhibiting stimuli alters axonal mRNA localization. (A and B) The changes in axonal levels of representative mRNAs in response to NGF, BDNF, and NT-3 (A) or MAG-Fc and Sema3A-AP (B) from Table I are graphically illustrated. Positive values indicate an increase in axonal mRNA content, and negative values indicate a decrease in axonal mRNA content detected by qPCR. The axonal mRNA values for neurotrophins are expressed relative to axons treated with BSA. Values for MAG are expressed relative to human IgG Fc, and those for Sema3A are expressed relative to AP (there were no statistically significant differences between the Fc and AP controls; not depicted). Error bars represent the SD of three replicate experiments with each sample measured in quadruplicate. Significance was calculated based on P ≤ 0.01 by a student Newman-Keul test compared with the control. Note that the neurotrophins, MAG, and Sema3A selectively increase or decrease the transport of individual mRNAs. No statistically significant changes were seen in axonal levels of αB crystallin, grp78/BiP, or HCN4 mRNAs with NGF, BDNF, or NT3 treatments. No statistically significant changes were seen in axonal levels of GAP43, Kv3.1a, or peripherin mRNAs with MAG or Sema3A treatments.

    Article Snippet: Pharmacological reagentsNGF (Harlan), BDNF, NT3 (Alomone Labs), MAG-Fc (R & D Systems), and Sema3A-AP ( ) were covalently coupled to 15-μm-diameter polystyrene microparticles according to the manufacturer's instructions (Polysciences).

    Techniques: Real-time Polymerase Chain Reaction

    K V current for low-frequency E18 NM neurons (E18 LF ). (A-B) Representative voltage-clamp traces to somatic current injections from −90pA to +30pA in +5pA increments from the control (A) and experimental (B) neurons. Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Averaged IV curve of the control (black triangles) and the experimental (white circles) NM neuron population. Each point is mean ± SEM. (D-G) K V current when the voltage is held at −60, −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 10 for control, N = 11 for NT-3.

    Journal: Neuroscience Insights

    Article Title: Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure

    doi: 10.1177/2633105520980442

    Figure Lengend Snippet: K V current for low-frequency E18 NM neurons (E18 LF ). (A-B) Representative voltage-clamp traces to somatic current injections from −90pA to +30pA in +5pA increments from the control (A) and experimental (B) neurons. Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Averaged IV curve of the control (black triangles) and the experimental (white circles) NM neuron population. Each point is mean ± SEM. (D-G) K V current when the voltage is held at −60, −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 10 for control, N = 11 for NT-3.

    Article Snippet: NT-3 was obtained from Alomone Labs (Jerusalem, Israel).

    Techniques:

    K V current for high-frequency E18 NM neurons (E18 HF ). (A-B) Representative voltage-clamp traces to somatic current injections ranging from −90pA to +30pA in +5pA increments from (A) control and (B) experimental (+NT-3) neurons. Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Population averaged I/V curves of the control (black triangles) and experimental (white circles) NM neurons. Data represents the mean ± SEM. (D-G) K V currents when the membrane voltage is held at −60, −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 11 for control, N = 9 for NT-3.

    Journal: Neuroscience Insights

    Article Title: Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure

    doi: 10.1177/2633105520980442

    Figure Lengend Snippet: K V current for high-frequency E18 NM neurons (E18 HF ). (A-B) Representative voltage-clamp traces to somatic current injections ranging from −90pA to +30pA in +5pA increments from (A) control and (B) experimental (+NT-3) neurons. Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Population averaged I/V curves of the control (black triangles) and experimental (white circles) NM neurons. Data represents the mean ± SEM. (D-G) K V currents when the membrane voltage is held at −60, −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 11 for control, N = 9 for NT-3.

    Article Snippet: NT-3 was obtained from Alomone Labs (Jerusalem, Israel).

    Techniques:

    K V current for high-frequency E13 NM neurons (E13 HF ). (A-B) Representative voltage-clamp traces to somatic current injections ranging from −90 pA to +30 pA in +5 pA increments from (A) control and (B) experimental (+NT-3) NM neurons. Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Population averaged I/V curves of control (black triangles) and the experimental (white circles) NM neurons. Data represents the mean ± SEM. (D-G) K V currents when the membrane voltage is held at −60, −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 11 for control, N = 9 for NT-3.

    Journal: Neuroscience Insights

    Article Title: Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure

    doi: 10.1177/2633105520980442

    Figure Lengend Snippet: K V current for high-frequency E13 NM neurons (E13 HF ). (A-B) Representative voltage-clamp traces to somatic current injections ranging from −90 pA to +30 pA in +5 pA increments from (A) control and (B) experimental (+NT-3) NM neurons. Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Population averaged I/V curves of control (black triangles) and the experimental (white circles) NM neurons. Data represents the mean ± SEM. (D-G) K V currents when the membrane voltage is held at −60, −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 11 for control, N = 9 for NT-3.

    Article Snippet: NT-3 was obtained from Alomone Labs (Jerusalem, Israel).

    Techniques:

    K V current for low-frequency E13 NM neurons (E13 LF ). (A-B) Representative voltage-clamp traces to somatic current injections ranging from −90pA to +30pA in +5pA increments from the control (A) and experimental neurons (B). Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Population averaged I/V curve of the control (black triangles) and experimental (white circles) NM neurons. Data represents the mean ± SEM. (D-G) K V currents when the membrane voltage is held at −60 (inset denotes smaller scale), −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 9 for control, N = 17 for NT-3. In this and the subsequent figures, P -values as indicated: *

    Journal: Neuroscience Insights

    Article Title: Effects of Neurotrophin-3 on Intrinsic Neuronal Properties at a Central Auditory Structure

    doi: 10.1177/2633105520980442

    Figure Lengend Snippet: K V current for low-frequency E13 NM neurons (E13 LF ). (A-B) Representative voltage-clamp traces to somatic current injections ranging from −90pA to +30pA in +5pA increments from the control (A) and experimental neurons (B). Symbols above current traces indicate region sampled to obtain steady state potassium currents. (C) Population averaged I/V curve of the control (black triangles) and experimental (white circles) NM neurons. Data represents the mean ± SEM. (D-G) K V currents when the membrane voltage is held at −60 (inset denotes smaller scale), −30, 0, and 30 mV. Each bar represents the mean value + SEM. N = 9 for control, N = 17 for NT-3. In this and the subsequent figures, P -values as indicated: *

    Article Snippet: NT-3 was obtained from Alomone Labs (Jerusalem, Israel).

    Techniques:

    NT3 staining of PV. Perisomatic endings are stained at all ages. Same symbols for cell types as in . Cochlear axons at P30 are heavily stained (thin arrow) along with clusters of nerve terminals at P30 and P8 and the incoming terminals at P14

    Journal: Journal of neuroscience research

    Article Title: Postnatal Development of NT3 and TrkC in Mouse Ventral Cochlear Nucleus

    doi: 10.1002/jnr.22179

    Figure Lengend Snippet: NT3 staining of PV. Perisomatic endings are stained at all ages. Same symbols for cell types as in . Cochlear axons at P30 are heavily stained (thin arrow) along with clusters of nerve terminals at P30 and P8 and the incoming terminals at P14

    Article Snippet: Anti-NT3 antibody was from Alomone Laboratories (Jerusalem, Israel), anti-TrkC was from R & D Systems (Min-neapolis, MN), and anti-SV2 was from the Developmental Studies Hybridoma Bank (Department of Biological Sciences, Iowa City, IA).

    Techniques: Staining

    Fluoresecent double labeling for NT3 (green) and SV2 (red) to show colocalization (yellow) in the PV region (P60). Left arrow, axons abundant with NT3; right arrow, NT3 in the perisomatic region. SV2 labels nerve terminals (double arrows); many of them

    Journal: Journal of neuroscience research

    Article Title: Postnatal Development of NT3 and TrkC in Mouse Ventral Cochlear Nucleus

    doi: 10.1002/jnr.22179

    Figure Lengend Snippet: Fluoresecent double labeling for NT3 (green) and SV2 (red) to show colocalization (yellow) in the PV region (P60). Left arrow, axons abundant with NT3; right arrow, NT3 in the perisomatic region. SV2 labels nerve terminals (double arrows); many of them

    Article Snippet: Anti-NT3 antibody was from Alomone Laboratories (Jerusalem, Israel), anti-TrkC was from R & D Systems (Min-neapolis, MN), and anti-SV2 was from the Developmental Studies Hybridoma Bank (Department of Biological Sciences, Iowa City, IA).

    Techniques: Labeling

    Comparison of perisomatic staining of NT3 and perikaryal location of TrkC in globular bushy cells (thick arrows; P30). Images taken at high magnification (×63 oil). Astrocytes are also stained for TrkC (thin arrows). Inset: A cell with granular

    Journal: Journal of neuroscience research

    Article Title: Postnatal Development of NT3 and TrkC in Mouse Ventral Cochlear Nucleus

    doi: 10.1002/jnr.22179

    Figure Lengend Snippet: Comparison of perisomatic staining of NT3 and perikaryal location of TrkC in globular bushy cells (thick arrows; P30). Images taken at high magnification (×63 oil). Astrocytes are also stained for TrkC (thin arrows). Inset: A cell with granular

    Article Snippet: Anti-NT3 antibody was from Alomone Laboratories (Jerusalem, Israel), anti-TrkC was from R & D Systems (Min-neapolis, MN), and anti-SV2 was from the Developmental Studies Hybridoma Bank (Department of Biological Sciences, Iowa City, IA).

    Techniques: Staining