Journal: International Journal of Molecular Sciences
Article Title: Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer’s Disease Brains
Figure Lengend Snippet: Different microglial morphologies associated with P2RY12 expression. Representative immunohistochemistry results of tissue sections stained to identify P2RY12 (purple) alone and Aβ (brown), IBA-1 or phosphorylated tau. ( A ). Ramified microglia in LPND case. ( B , C ). Microglia with fragmented morphology in HPND cases. ( C ). Fragmented microglia associated with diffuse Aβ plaques. ( D ). P2RY12 microglia with tufted morphology in AD case. ( E , F ). Colocalization of P2RY12 and IBA-1. ( E ). Rod shaped IBA-1-positive microglia (brown arrow) with minimal P2RY12 immunoreactivity. P2RY12-positive, IBA-1-positive microglia (arrowheads ( E , F )). All P2RY12 immunoreactive microglia showed some IBA-1 immunoreactivity. ( F ). IBA-1 positive cluster surrounded by P2RY12 microglia. ( G – I ). Different morphologies of P2RY12-positive microglia interacting with Aβ plaques. ( G ). P2RY12-positive microglia with long processes interacting with diffuse plaques in HPND case. ( H , I ). P2RY12-positve microglia with activated morphologies (large cell bodies, short processes) interacting with dense Aβ plaques. ( J , K ). P2RY12-positive rod-shaped microglia in LPND ( J ) and HPND ( K ) sections. ( L ) P2RY12-positive microglial processes show interactions with neurons. Scale bars represent 50 μm.
Article Snippet: In addition, comparisons of immunostaining patterns were carried using an independent antibody to P2RY12 (1:250, Alomone Labs), prepared against an 18-amino acid peptide sequence that did not overlap with the immunizing sequence of the Novus antibody.
Techniques: Expressing, Immunohistochemistry, Staining