Dmso, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing"
Article Title: Dysfunctional TRPM8 signalling in the vascular response to environmental cold in ageing
Figure Legend Snippet: TRPA1 and TRPM8 are involved in cold-induced vascular response. Vascular responses with cold (4 °C) water treatment in mice pre-treated with combined TRPA1 antagonist A967079 (100 mg kg –1 ) and TRPM8 antagonist AMTB (10 mg kg –1 ), or vehicle control (Veh - 10 % DMSO, 10 % Tween in saline) i.p. 30 min before cold treatment. ( a–c ) % change in hindpaw blood flow from baseline to 0–2 min following cold treatment (maximum vasoconstriction) in mice treated with combined antagonist ( a ) A967079+ AMTB, ( b ) A967079, and ( c ) AMTB. ( d-f ) Maximum vasoconstriction caused by cold water treatment in mice treated with combined antagonist ( d ) A967079+ AMTB, ( e ) A967079, and ( f ) AMTB normalised against vehicle treated mice. (n = 5–10) ( g–h ) RT-PCR CT analysis shows fold change of ( g ) Trpa1 and ( h ) Trpm8 normalised to three housekeeping genes in DRG. (n = 14–15) ( i ) Representative western blot of TRPM8 in DRG of young and aged mice and densitometric analysis normalised to Tubulin (Y = young, A = aged) (n = 9). All results are shown as mean ± s.e.m. *p
Techniques Used: Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot