ana 12  (Alomone Labs)

Journal: iScience

Article Title: Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels

doi: 10.1016/j.isci.2021.103018

Figure Lengend Snippet: Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).

Article Snippet: ML297 (Alomone Labs; M-215) was dissolved in 100% DMSO to a final concentration of 25 mM.

Techniques: Activity Assay, Activation Assay, Expressing

Journal: bioRxiv

Article Title: Gain control by sparse, ultra-slow glycinergic synapses

doi: 10.1101/2021.05.03.442480

Figure Lengend Snippet: GlyR α 4 receptors in SACs mediate slow and delayed sIPSCs. A. sIPSCs measured from ON SACs in wild-type mice at 0 mV in control ( left panel ). Normalized average fast (blue) and slow (red) sIPSCs ( middle panel ). Frequency distribution of rise, decay time and amplitude of the population events ( right panels ; n = 5 SACs). B-C . Same as A, but in gabazine (B) and strychnine (C) in wild-type SACs. D. same as A B except SAC recordings were made in Glra4 KO retina.

Article Snippet: TTX and gabazine were purchased from Alamone Labs and Tocris, respectively.

Techniques: Mouse Assay

Journal: bioRxiv

Article Title: Gain control by sparse, ultra-slow glycinergic synapses

doi: 10.1101/2021.05.03.442480

Figure Lengend Snippet: Glycine, not GABA receptors, mediate the dominant form of inhibition to SACs. A. IPSCs recorded from an ON SAC (at ∼0mV) evoked by small stationary spots (2 s;100 μm diameter) in control (black), in the presence of GlyR antagonist (1 µM strychnine, red) and following addition of the GABA A R antagonist, gabazine (grey). Inset shows the transient response in the boxed region at higher magnification. B. Average ON ( top and middle ) and OFF ( bottom ) inhibitory responses measured across 6 cells (black-mean ± SEM, grey-individual cells) in three conditions as shown in A. The middle panel plots the same data as the top panel and is magnified to highlight the significant reduction of ON inhibitory charge in gabazine. (* p

Article Snippet: TTX and gabazine were purchased from Alamone Labs and Tocris, respectively.

Techniques: Inhibition