Journal: eLife

Article Title: A tonic nicotinic brake controls spike timing in striatal spiny projection neurons

doi: 10.7554/eLife.75829

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Article Snippet: Chemical compound, drug , Naspm trihydrochloride , Alomone Labs , CAS: 1049731-36-3 , .

Techniques: Plasmid Preparation, Recombinant

Journal: iScience

Article Title: Encephalopathy-causing mutations in Gβ 1 ( GNB1 ) alter regulation of neuronal GIRK channels

doi: 10.1016/j.isci.2021.103018

Figure Lengend Snippet: Rescue of GIRK channel activity by VU0529331 and ML297 in the presence of LoF mutants I80N and I80T Oocytes expressed GIRK2 (2 ng RNA) or GIRK1/3 (3 ng) channels, with or without Gβ WT (5 ng), I80N and I80T (10 ng), and Gγ (1/5 of Gβ). (A) Representative records of GIRK2 currents and their activation by 40 μM of VU0529331 in oocytes expressing Gβγ. (B and C) Summary of GIRK2 currents with and without Gβγ before (B) and after (C) the application of 40 μM VU0529331. (D) Fold activation of GIRK2 by 40 μM of VU0529331. (E) GIRK1/3 currents and their activation by 10 μM of ML297 in the presence of Gβγ. (F and G) Summary of GIRK1/3 currents with and without Gβγ before (F) and after (G) the application of 10 μM ML297. (H) Fold activation of GIRK1/3 by 10 μM of ML297 (N = 1).

Article Snippet: ML297 , Alomone labs , M-215.

Techniques: Activity Assay, Activation Assay, Expressing

Journal: iScience

Article Title: Encephalopathy-causing mutations in Gβ 1 ( GNB1 ) alter regulation of neuronal GIRK channels

doi: 10.1016/j.isci.2021.103018

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Article Snippet: ML297 , Alomone labs , M-215.

Techniques: Recombinant, Modification, Mutagenesis, Software

Journal: Nature cell biology

Article Title: STAT3-BDNF-TrkB signaling promotes alveolar epithelial regeneration after lung injury

doi: 10.1038/s41556-020-0569-x

Figure Lengend Snippet: (A-C) Analysis of scRNA-seq of mesenchymal cells from uninjured mice showed that TrkB expression is enriched in mesenchymal alveolar niche cells. (D) Ingenuity pathways analysis show that mesenchymal cells expressing TrkB are enriched with expression of genes that control respiratory system development, organ development and tissue morphology. (E) Expression of TrkB in GFP+ and GFP− cells from TrkBEGFP mice (n=4 mice per group). (F) Expression of TrkB on mesenchymal cells was verified by staining lung tissue form TrkBEGFP mice for PDFRα. (G) Quantification of GFP and PDFRα co-positive cells in TrkBEGFP mice 24 hours after acid-induced lung injury (n=4 mice per group) The gating strategy is shown in Extended Figure 8. (H & I) AT2 cells isolated from a tamoxifen treated SftpcCreERT2-Rosa26mTmG mouse were co-cultured with PDGFRα+ mesenchymal cells for four weeks. ANA-12 abrogated organoid forming efficiency of AT2 cells (n=3 wells per condition). (J & K) The organoid forming capacity of primary human AT2 cells was abrogated by the addition of ANA-12 to the media. Cultures were grown for four weeks. Right-hand panels show average organoid forming efficiency and size from n=3 different donors. For panels (E), (G), (K) and (I), data is shown as the mean +/− SEM. Statistical significance was determined with a two-tailed Student’s t-test (E), (G), (K) and (I). Scale bar, 2.6mm (H, J), 20μm (F).

Article Snippet: After two days of culture, Y276632 was removed and ligand treatments of organoids were performed using the following reagents at the indicated concentrations: ANA-12 0.02μg/mL (Alomone Labs catalog #A-215), recombinant human BDNF 0.05μg/mL (Alomone Labs catalog #B-250) and recombinant murine FGF7 0.01μg/mL (R&D Systems catalog #5028-KG).

Techniques: Expressing, Staining, Isolation, Cell Culture, Two Tailed Test