Isradipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels"
Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels
Journal: Molecular psychiatry
Figure Legend Snippet: LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p
Techniques Used: Conditioned Place Preference, SPR Assay, Mouse Assay
Figure Legend Snippet: Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p
Techniques Used: Mouse Assay, Injection, Expressing, Imaging, SPR Assay, Blocking Assay
2) Product Images from "CaMKII inactivation by extracellular Ca2+ depletion in dorsal root ganglion neurons"
Article Title: CaMKII inactivation by extracellular Ca2+ depletion in dorsal root ganglion neurons
Journal: Cell calcium
Figure Legend Snippet: Activation of CaMKII is independent of VGCC-mediated Ca 2+ influx. Pre-incubation with either the L-type channel blockers isradipine or SR 33805 ( n = 6) at 10 μM failed to block CaMKII activation. Additionally, the Na + /Ca 2+ -exchanger inhibitor KB-R7943 at 10 μM ( n = 6) also did not affect activation of CaMKII by Ca 2+ O -stimulation. Basal phosphorylation at 286/287 Thr in 0 Ca 2+ was 12 ± 1% of stimulation by 1.8 Ca 2+ O . Neither compound significantly affected Ca 2+ -stimulation ( n = 6) suggesting that changes in [Ca 2+ ] O act at a site independent of VGCC.
Techniques Used: Activation Assay, Incubation, Blocking Assay, Activated Clotting Time Assay