Journal: Journal of Cellular and Molecular Medicine
Article Title: Up‐regulation of miR‐195 contributes to cardiac hypertrophy‐induced arrhythmia by targeting calcium and potassium channels, et al. Up‐regulation of miR‐195 contributes to cardiac hypertrophy‐induced arrhythmia by targeting calcium and potassium channels
Figure Lengend Snippet: Direct interaction between miR‐195 and Cavβ1, Kir2.1 and Kv4.3. (A) Direct interaction between miR‐195 and Cavβ1. A fragment of miR‐195 that binds to CACNB1. miR‐195 is complementary to the CACNB1 gene 943‐949, which encodes Cavβ1, and the corresponding mutant sequence is designed based on the binding site. (B) Luciferase reporter with a CACNB1 fragment capable of binding to miR‐195. The gene was co‐transfected with miR‐195 into HEK293 cells, and miR‐195 reduced the activity of the luciferase reporter gene, ** P
Article Snippet: After the addition of the miR‐195 inhibitor, the down‐regulation of Cavβ1 (#P < .05 vs. miR‐195 mimics), Kir2.1 (#P < .05 vs. miR‐195) and Kv4.3 (#P < .05 vs. miR‐195) was restored to normal level (Figure ,E).
Techniques: Mutagenesis, Sequencing, Binding Assay, Luciferase, Transfection, Activity Assay