amg9810  (Alomone Labs)


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  • 90

    Structured Review

    Alomone Labs amg9810
    Amg9810, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amg9810/product/Alomone Labs
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    amg9810 - by Bioz Stars, 2022-12
    90/100 stars

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    Alomone Labs β pompilidotoxin pomp
    Effect of tefluthin, β-pompilidoxin, tefluthrin plus sparsentan, <t>β-pompilidotoxin</t> plus sparsentan on I Na in GH 3 cells. The voltage-clamp experiments were undertaken, as cells were voltage-clamped at −80 mV and the depolarizing pulse to −10 mV was imposed to them. ( A ) Representative current traces obtained in the control period (a, sparsentan was not present), and during exposure to 3 μM sparsentan (b) or to 3 μM sparsentan plus 3 μM tefluthrin (c). The top panel indicates the voltage-clamp protocol applied. Of note, the deactivating I Na was additionally detected as membrane potential was returned to −50 mV with a duration of 40 ms. ( B ) Summary bar graph demonstrating effect of 3 μM tefluthrin, 3 μM β-pompilidotoxn, 3 μM tefluthrin plus 3 μM sparsentan and 3 μM β-pompilidotoxin plus 3 μM sparsentan on the peak amplitude of I Na (mean ± SEM; n = 8 for each bar). The peak amplitude was measured at the start of 40 ms depolarizing command voltage from −80 to −10 mV. Tef: tefluthrin; Pomp: β-pompilidotoxin; Spar, sparsentan. Statistical analysis was performed by one-way ANOVA ( p
    β Pompilidotoxin Pomp, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    93
    Alomone Labs ns309
    PV RBCs show increased Gárdos channel activity. ( A ) CCCP method analysis of PV and CT RBC membrane potential changes upon 100 µM <t>NS309</t> addition. At the end of the experiment, cells were lysed with 3M NaCl 1% Triton X lysis solution to obtain the zero membrane potential (pHi = pHo) for absolute calculation of membrane potential. CT (mean—black line; SD—grey) and PV (mean—red line; SD—pink) RBCs. Data are displayed as mean with 95% confidence interval; CT ( n = 6) and PV ( n = 8). ( B ) Cell volume assay on Gárdos activity; 0.05% RBCs suspension was prepared in PBS with a final concertation of 0.2% BSA, 1 mM CaCl 2 , and 100 µM NS309. RBC size was measured using CASY before and 2.5, 5, 7.5, and 10 min after 100 μM NS309 addition. Mean with SD, n = 6, Mann–Whitney test. ( C – E ) Patch-clamp analysis. Upon cell catch external solution was added to the wells followed by 10 µM NS3623, 10 µM NS309, 5 µM TRAM-34, and 30 µM GdCl 3 . Currents were measured at room temperature applying −100 to +80 mV ramp voltage protocol for 300 ms, at a holding potential of −30 mV. The cell response was measured in pA at +80 mV. Statistical analysis of the currents at +80 mV in NS309 and TRAM-34 responding cells in ( C ) CT and PV RBCs ( n = 17; n = 48, respectively), ( D ) BaF3 EpoR JAK2 WT ( n = 20) and BaF3 EpoR JAK2 V617F ( n = 48), and ( E ) HEL ( n = 61) and HEL cells treated with 0.3 µM ruxolitinib for 24 h ( n = 63). Cell was considered responsive if it displayed at least a 20% current change. The left panel represents the cell current upon the addition of NS3623 (baseline), NS309, and TRAM-34. The central panel displays NS309-induced current increase, while the right panels represent TRAM-34-induced current decrease. The data are presented as median and box plots (25–75%) with whiskers (10–90%). Mann–Whitney test or Wilcoxon test, * p
    Ns309, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    Effect of tefluthin, β-pompilidoxin, tefluthrin plus sparsentan, β-pompilidotoxin plus sparsentan on I Na in GH 3 cells. The voltage-clamp experiments were undertaken, as cells were voltage-clamped at −80 mV and the depolarizing pulse to −10 mV was imposed to them. ( A ) Representative current traces obtained in the control period (a, sparsentan was not present), and during exposure to 3 μM sparsentan (b) or to 3 μM sparsentan plus 3 μM tefluthrin (c). The top panel indicates the voltage-clamp protocol applied. Of note, the deactivating I Na was additionally detected as membrane potential was returned to −50 mV with a duration of 40 ms. ( B ) Summary bar graph demonstrating effect of 3 μM tefluthrin, 3 μM β-pompilidotoxn, 3 μM tefluthrin plus 3 μM sparsentan and 3 μM β-pompilidotoxin plus 3 μM sparsentan on the peak amplitude of I Na (mean ± SEM; n = 8 for each bar). The peak amplitude was measured at the start of 40 ms depolarizing command voltage from −80 to −10 mV. Tef: tefluthrin; Pomp: β-pompilidotoxin; Spar, sparsentan. Statistical analysis was performed by one-way ANOVA ( p

    Journal: Biomedicines

    Article Title: The Evidence for Sparsentan-Mediated Inhibition of INa and IK(erg): Possibly Unlinked to Its Antagonism of Angiotensin II or Endothelin Type a Receptor

    doi: 10.3390/biomedicines10010086

    Figure Lengend Snippet: Effect of tefluthin, β-pompilidoxin, tefluthrin plus sparsentan, β-pompilidotoxin plus sparsentan on I Na in GH 3 cells. The voltage-clamp experiments were undertaken, as cells were voltage-clamped at −80 mV and the depolarizing pulse to −10 mV was imposed to them. ( A ) Representative current traces obtained in the control period (a, sparsentan was not present), and during exposure to 3 μM sparsentan (b) or to 3 μM sparsentan plus 3 μM tefluthrin (c). The top panel indicates the voltage-clamp protocol applied. Of note, the deactivating I Na was additionally detected as membrane potential was returned to −50 mV with a duration of 40 ms. ( B ) Summary bar graph demonstrating effect of 3 μM tefluthrin, 3 μM β-pompilidotoxn, 3 μM tefluthrin plus 3 μM sparsentan and 3 μM β-pompilidotoxin plus 3 μM sparsentan on the peak amplitude of I Na (mean ± SEM; n = 8 for each bar). The peak amplitude was measured at the start of 40 ms depolarizing command voltage from −80 to −10 mV. Tef: tefluthrin; Pomp: β-pompilidotoxin; Spar, sparsentan. Statistical analysis was performed by one-way ANOVA ( p

    Article Snippet: Angiotensin II (AngII), endothelin 1, tefluthrin (Tef), tetraethylammonium chloride (TEA) were supplied by Sigma-Aldrich (Merck, Taipei, Taiwan) and β-pompilidotoxin (Pomp) and tetrodotoxin (TTX) were by Alomone (Jerusalem, Israel).

    Techniques:

    PV RBCs show increased Gárdos channel activity. ( A ) CCCP method analysis of PV and CT RBC membrane potential changes upon 100 µM NS309 addition. At the end of the experiment, cells were lysed with 3M NaCl 1% Triton X lysis solution to obtain the zero membrane potential (pHi = pHo) for absolute calculation of membrane potential. CT (mean—black line; SD—grey) and PV (mean—red line; SD—pink) RBCs. Data are displayed as mean with 95% confidence interval; CT ( n = 6) and PV ( n = 8). ( B ) Cell volume assay on Gárdos activity; 0.05% RBCs suspension was prepared in PBS with a final concertation of 0.2% BSA, 1 mM CaCl 2 , and 100 µM NS309. RBC size was measured using CASY before and 2.5, 5, 7.5, and 10 min after 100 μM NS309 addition. Mean with SD, n = 6, Mann–Whitney test. ( C – E ) Patch-clamp analysis. Upon cell catch external solution was added to the wells followed by 10 µM NS3623, 10 µM NS309, 5 µM TRAM-34, and 30 µM GdCl 3 . Currents were measured at room temperature applying −100 to +80 mV ramp voltage protocol for 300 ms, at a holding potential of −30 mV. The cell response was measured in pA at +80 mV. Statistical analysis of the currents at +80 mV in NS309 and TRAM-34 responding cells in ( C ) CT and PV RBCs ( n = 17; n = 48, respectively), ( D ) BaF3 EpoR JAK2 WT ( n = 20) and BaF3 EpoR JAK2 V617F ( n = 48), and ( E ) HEL ( n = 61) and HEL cells treated with 0.3 µM ruxolitinib for 24 h ( n = 63). Cell was considered responsive if it displayed at least a 20% current change. The left panel represents the cell current upon the addition of NS3623 (baseline), NS309, and TRAM-34. The central panel displays NS309-induced current increase, while the right panels represent TRAM-34-induced current decrease. The data are presented as median and box plots (25–75%) with whiskers (10–90%). Mann–Whitney test or Wilcoxon test, * p

    Journal: Cells

    Article Title: Altered Ca2+ Homeostasis in Red Blood Cells of Polycythemia Vera Patients Following Disturbed Organelle Sorting during Terminal Erythropoiesis

    doi: 10.3390/cells11010049

    Figure Lengend Snippet: PV RBCs show increased Gárdos channel activity. ( A ) CCCP method analysis of PV and CT RBC membrane potential changes upon 100 µM NS309 addition. At the end of the experiment, cells were lysed with 3M NaCl 1% Triton X lysis solution to obtain the zero membrane potential (pHi = pHo) for absolute calculation of membrane potential. CT (mean—black line; SD—grey) and PV (mean—red line; SD—pink) RBCs. Data are displayed as mean with 95% confidence interval; CT ( n = 6) and PV ( n = 8). ( B ) Cell volume assay on Gárdos activity; 0.05% RBCs suspension was prepared in PBS with a final concertation of 0.2% BSA, 1 mM CaCl 2 , and 100 µM NS309. RBC size was measured using CASY before and 2.5, 5, 7.5, and 10 min after 100 μM NS309 addition. Mean with SD, n = 6, Mann–Whitney test. ( C – E ) Patch-clamp analysis. Upon cell catch external solution was added to the wells followed by 10 µM NS3623, 10 µM NS309, 5 µM TRAM-34, and 30 µM GdCl 3 . Currents were measured at room temperature applying −100 to +80 mV ramp voltage protocol for 300 ms, at a holding potential of −30 mV. The cell response was measured in pA at +80 mV. Statistical analysis of the currents at +80 mV in NS309 and TRAM-34 responding cells in ( C ) CT and PV RBCs ( n = 17; n = 48, respectively), ( D ) BaF3 EpoR JAK2 WT ( n = 20) and BaF3 EpoR JAK2 V617F ( n = 48), and ( E ) HEL ( n = 61) and HEL cells treated with 0.3 µM ruxolitinib for 24 h ( n = 63). Cell was considered responsive if it displayed at least a 20% current change. The left panel represents the cell current upon the addition of NS3623 (baseline), NS309, and TRAM-34. The central panel displays NS309-induced current increase, while the right panels represent TRAM-34-induced current decrease. The data are presented as median and box plots (25–75%) with whiskers (10–90%). Mann–Whitney test or Wilcoxon test, * p

    Article Snippet: Gárdos channel activity in the presence of NS309 was significantly increased in BaF3 JAK2V617F cells (n = 48) compared with in BaF3 JAK2WT (n = 20) ( D left and middle panels).

    Techniques: Activity Assay, Lysis, MANN-WHITNEY, Patch Clamp