trypsin  (ATCC)


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    Structured Review

    ATCC trypsin
    Trypsin, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 10 article reviews
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    trypsin - by Bioz Stars, 2022-10
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    ATCC penicillin streptomycin amphotericin b solution
    Analysis of the potential of fenbendazole to control animal cryptococcosis. A-D. Antifungal effects of fenbendazole and other benzimidazoles in comparison with their liver metabolites. All benzimidazoles had clear antifungal activity, in contrast to their liver metabolites of fenbendazole (A), mebendazole (B), flubendazole (C) and albendazole (D). E. Treatment of lethally infected mice with intranasally delivered fenbendazole or intraperitoneally administered <t>amphotericin</t> B (AmB). All vehicle-treated animals died 26 days post-infection. Drug-treated animals were all alive 27 days post infection.
    Penicillin Streptomycin Amphotericin B Solution, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC human vaginal epithelial hve cells
    (A) Optical images (10× scale, 2 μm) and (B) cell proliferation (by <t>MTT</t> assay) of <t>HVE</t> cells after treatment with FLG-C (FLG), NR latex thin film, and FLG-C-NR latex thin film. The arrows indicate the presence of FLG.
    Human Vaginal Epithelial Hve Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Analysis of the potential of fenbendazole to control animal cryptococcosis. A-D. Antifungal effects of fenbendazole and other benzimidazoles in comparison with their liver metabolites. All benzimidazoles had clear antifungal activity, in contrast to their liver metabolites of fenbendazole (A), mebendazole (B), flubendazole (C) and albendazole (D). E. Treatment of lethally infected mice with intranasally delivered fenbendazole or intraperitoneally administered amphotericin B (AmB). All vehicle-treated animals died 26 days post-infection. Drug-treated animals were all alive 27 days post infection.

    Journal: bioRxiv

    Article Title: Fenbendazole controls in vitro growth, virulence potential and animal infection in the Cryptococcus model

    doi: 10.1101/2020.02.13.948745

    Figure Lengend Snippet: Analysis of the potential of fenbendazole to control animal cryptococcosis. A-D. Antifungal effects of fenbendazole and other benzimidazoles in comparison with their liver metabolites. All benzimidazoles had clear antifungal activity, in contrast to their liver metabolites of fenbendazole (A), mebendazole (B), flubendazole (C) and albendazole (D). E. Treatment of lethally infected mice with intranasally delivered fenbendazole or intraperitoneally administered amphotericin B (AmB). All vehicle-treated animals died 26 days post-infection. Drug-treated animals were all alive 27 days post infection.

    Article Snippet: Similar protocols were used for determination of MICs for amphotericin B, fluconazole and benzimidazole derivatives.

    Techniques: Activity Assay, Infection, Mouse Assay

    Antifungal effect and toxicity of fenbendazole (FBZ). A. Determination of the minimum inhibitory concentration (MIC) of fenbendazole against C. neoformans (strain H99) and C. gattii (strain R265). Both strains were sensitive to a MIC of 0.012 μg/ml (boxed area). B. FBZ is fungicidal, as concluded from its ability to drastically reduce the numbers of colony forming units (CFU) of C. neoformans and C. gattii at the MIC. C. Dose-dependent profile of toxicity of fenbendazole against RAW 264.7 macrophages. The dotted line represents the 50% cut off of cellular viability. D. Antifungal effects of subinhibitory doses of fenbendazole (0.003 and 0.006 μg/ml) in combination with variable concentrations of amphotericin B (AmB) against C. neoformans and C. gattii . In both cases, the presence of fenbendazole results in decreased concentrations of amphotericin B required for growth inhibition. E. Analysis of the potential of fenbendazole and amphotericin B alone or in combination to kill RAW 264.7 macrophages. Control cells consisted of mammalian cultures treated with vehicle (DMSO) alone. Lysis control consisted of cells treated with 1x lysis solution (provided by the manufacturer). No significant (n.s.) differences were found between the antifungal preparations.

    Journal: bioRxiv

    Article Title: Fenbendazole controls in vitro growth, virulence potential and animal infection in the Cryptococcus model

    doi: 10.1101/2020.02.13.948745

    Figure Lengend Snippet: Antifungal effect and toxicity of fenbendazole (FBZ). A. Determination of the minimum inhibitory concentration (MIC) of fenbendazole against C. neoformans (strain H99) and C. gattii (strain R265). Both strains were sensitive to a MIC of 0.012 μg/ml (boxed area). B. FBZ is fungicidal, as concluded from its ability to drastically reduce the numbers of colony forming units (CFU) of C. neoformans and C. gattii at the MIC. C. Dose-dependent profile of toxicity of fenbendazole against RAW 264.7 macrophages. The dotted line represents the 50% cut off of cellular viability. D. Antifungal effects of subinhibitory doses of fenbendazole (0.003 and 0.006 μg/ml) in combination with variable concentrations of amphotericin B (AmB) against C. neoformans and C. gattii . In both cases, the presence of fenbendazole results in decreased concentrations of amphotericin B required for growth inhibition. E. Analysis of the potential of fenbendazole and amphotericin B alone or in combination to kill RAW 264.7 macrophages. Control cells consisted of mammalian cultures treated with vehicle (DMSO) alone. Lysis control consisted of cells treated with 1x lysis solution (provided by the manufacturer). No significant (n.s.) differences were found between the antifungal preparations.

    Article Snippet: Similar protocols were used for determination of MICs for amphotericin B, fluconazole and benzimidazole derivatives.

    Techniques: Concentration Assay, Inhibition, Lysis

    Effects of fenbendazole (FBZ) on the intracellular fate of Cryptococcus . Treatment with fenbendazole decreased the intracellular proliferation rates of C. neoformans (A) and C. gattii (B). Similar profiles of inhibitory effects were observed when vomocytosis was analyzed in macrophages infected with C. neoformans (C) or C. gattii (D). The recovery of significantly lower viable fungal cells from FBZ-treated macrophages (0.012 μg/ml) indicated intracellular activity against C. neoformans (E) and C. gattii (F). Similar results were obtained with an antifungal concentration range of amphotericin B (AmB) or with a combination of subinhibitory doses of AmB and FBZ. **P = 0.01; ***P = 0.001; ****P

    Journal: bioRxiv

    Article Title: Fenbendazole controls in vitro growth, virulence potential and animal infection in the Cryptococcus model

    doi: 10.1101/2020.02.13.948745

    Figure Lengend Snippet: Effects of fenbendazole (FBZ) on the intracellular fate of Cryptococcus . Treatment with fenbendazole decreased the intracellular proliferation rates of C. neoformans (A) and C. gattii (B). Similar profiles of inhibitory effects were observed when vomocytosis was analyzed in macrophages infected with C. neoformans (C) or C. gattii (D). The recovery of significantly lower viable fungal cells from FBZ-treated macrophages (0.012 μg/ml) indicated intracellular activity against C. neoformans (E) and C. gattii (F). Similar results were obtained with an antifungal concentration range of amphotericin B (AmB) or with a combination of subinhibitory doses of AmB and FBZ. **P = 0.01; ***P = 0.001; ****P

    Article Snippet: Similar protocols were used for determination of MICs for amphotericin B, fluconazole and benzimidazole derivatives.

    Techniques: Infection, Activity Assay, Concentration Assay

    Antifungal activity of amphotericin B, caspofungin, fluconazole and voriconazole against  D. rugosa  complex planktonic cells.

    Journal: bioRxiv

    Article Title: Diutina (Candida) rugosa complex: The biofilm ultrastructure, extracellular matrix, cell wall component and antifungal susceptibility to amphotericin B, caspofungin, fluconazole and voriconazole

    doi: 10.1101/2020.03.30.016246

    Figure Lengend Snippet: Antifungal activity of amphotericin B, caspofungin, fluconazole and voriconazole against D. rugosa complex planktonic cells.

    Article Snippet: The MIC50 of Diutina complex planktonics cells were 32 µg/mL for amphotericin B, 8 µg/mL for caspofungin, 64 to 128 µg/mL for fluconazole and 256 to 2048 µg/mL voriconazole. shows the graphs for the antifungal susceptibility tests against Diutina complex planktonic cells.

    Techniques: Activity Assay

    Antifungal activity of amphotericin B, caspofungin, fluconazole and voriconazole against  D. rugosa  complex biofilm.

    Journal: bioRxiv

    Article Title: Diutina (Candida) rugosa complex: The biofilm ultrastructure, extracellular matrix, cell wall component and antifungal susceptibility to amphotericin B, caspofungin, fluconazole and voriconazole

    doi: 10.1101/2020.03.30.016246

    Figure Lengend Snippet: Antifungal activity of amphotericin B, caspofungin, fluconazole and voriconazole against D. rugosa complex biofilm.

    Article Snippet: The MIC50 of Diutina complex planktonics cells were 32 µg/mL for amphotericin B, 8 µg/mL for caspofungin, 64 to 128 µg/mL for fluconazole and 256 to 2048 µg/mL voriconazole. shows the graphs for the antifungal susceptibility tests against Diutina complex planktonic cells.

    Techniques: Activity Assay

    (A) Optical images (10× scale, 2 μm) and (B) cell proliferation (by MTT assay) of HVE cells after treatment with FLG-C (FLG), NR latex thin film, and FLG-C-NR latex thin film. The arrows indicate the presence of FLG.

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Cytotoxicity of Formulated Graphene and Its Natural Rubber Nanocomposite Thin Film in Human Vaginal Epithelial Cells: An Influence of Noncovalent Interaction

    doi: 10.1021/acsbiomaterials.9b01897

    Figure Lengend Snippet: (A) Optical images (10× scale, 2 μm) and (B) cell proliferation (by MTT assay) of HVE cells after treatment with FLG-C (FLG), NR latex thin film, and FLG-C-NR latex thin film. The arrows indicate the presence of FLG.

    Article Snippet: HeLA and HVE Cells Viability by [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) Assay Initially, HeLA cells of density 5000 cells/well were taken in a 96-well plate and incubated at 37 °C in a CO2 incubator for 24 h. Twenty microliters of FLG-C(FLG) [0(0), 50(12), 100(24), 250(60), and 500(121) μg/mL]/extracts of NR latex and FLG-C-NR latex thin film (0, 1:1, 1:2, 1:4, 1:8, and 1:16 dilution)/additives (0, 50, 100, 250, 500, and 1000 μg/mL) were added and incubated at 37 °C in the CO2 incubator for 48 h. Then, the MTT assay on human vaginal epithelial (HVE) cells [obtained from ATCC (American Type Culture Collection), #PCS-999-003, #PCS-999-004] was conducted as described above with slight modifications, such as 4000 cells/well in 96-well plates and incubated overnight in 200 μL of media (ATCC, #PCS-480-040 and #PCS-480-030).

    Techniques: MTT Assay