Journal: ACS Biomaterials Science & Engineering
Article Title: Cytotoxicity of Formulated Graphene and Its Natural Rubber Nanocomposite Thin Film in Human Vaginal Epithelial Cells: An Influence of Noncovalent Interaction
Figure Lengend Snippet: (A) Optical images (10× scale, 2 μm) and (B) cell proliferation (by MTT assay) of HVE cells after treatment with FLG-C (FLG), NR latex thin film, and FLG-C-NR latex thin film. The arrows indicate the presence of FLG.
Article Snippet: HeLA and HVE Cells Viability by [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) Assay Initially, HeLA cells of density 5000 cells/well were taken in a 96-well plate and incubated at 37 °C in a CO2 incubator for 24 h. Twenty microliters of FLG-C(FLG) [0(0), 50(12), 100(24), 250(60), and 500(121) μg/mL]/extracts of NR latex and FLG-C-NR latex thin film (0, 1:1, 1:2, 1:4, 1:8, and 1:16 dilution)/additives (0, 50, 100, 250, 500, and 1000 μg/mL) were added and incubated at 37 °C in the CO2 incubator for 48 h. Then, the MTT assay on human vaginal epithelial (HVE) cells [obtained from ATCC (American Type Culture Collection), #PCS-999-003, #PCS-999-004] was conducted as described above with slight modifications, such as 4000 cells/well in 96-well plates and incubated overnight in 200 μL of media (ATCC, #PCS-480-040 and #PCS-480-030).
Techniques: MTT Assay