phenomaster  (TSE systems)


Bioz Verified Symbol TSE systems is a verified supplier
Bioz Manufacturer Symbol TSE systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    TSE systems phenomaster
    Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the <t>PhenoMaster</t> ( Fig. 3 D). * p
    Phenomaster, supplied by TSE systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenomaster/product/TSE systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenomaster - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice"

    Article Title: Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice

    Journal: Behavioural Brain Research

    doi: 10.1016/j.bbr.2013.04.041

    Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the PhenoMaster ( Fig. 3 D). * p
    Figure Legend Snippet: Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the PhenoMaster ( Fig. 3 D). * p

    Techniques Used: Activity Assay, Mouse Assay

    Habituation of Mecp2 Stop and WT mice to novel home cages recorded by the PhenoMaster. Animals were recorded upon introduction to the novel cage in 10-min bins for 3 h (habituation complete in WT mice). Mean ± SEM. (A) Activity profiling confirmed higher activity in WT during early phases of habituation (asterisks are for 10–30 min, p
    Figure Legend Snippet: Habituation of Mecp2 Stop and WT mice to novel home cages recorded by the PhenoMaster. Animals were recorded upon introduction to the novel cage in 10-min bins for 3 h (habituation complete in WT mice). Mean ± SEM. (A) Activity profiling confirmed higher activity in WT during early phases of habituation (asterisks are for 10–30 min, p

    Techniques Used: Mouse Assay, Activity Assay

    Home cage activity as recorded by PhenoMaster. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active during the dark phase with a strong locomotor deficit apparent at 2–6 h of the dark phase. Indeed, nocturnal activity was significantly higher in WT mice (Bi), but not during light phases (Bii), and was due to reductions in both ambulatory (Ci) and fine movements (Cii). Automatically monitored food intake was higher in Mecp2 Stop mice on all recording days (Di) and increased cumulative food intake over 3 days was due to nocturnal eating (Dii). * p
    Figure Legend Snippet: Home cage activity as recorded by PhenoMaster. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active during the dark phase with a strong locomotor deficit apparent at 2–6 h of the dark phase. Indeed, nocturnal activity was significantly higher in WT mice (Bi), but not during light phases (Bii), and was due to reductions in both ambulatory (Ci) and fine movements (Cii). Automatically monitored food intake was higher in Mecp2 Stop mice on all recording days (Di) and increased cumulative food intake over 3 days was due to nocturnal eating (Dii). * p

    Techniques Used: Activity Assay, Mouse Assay

    2) Product Images from "A BAFF/APRIL axis regulates obesogenic diet-driven weight gain"

    Article Title: A BAFF/APRIL axis regulates obesogenic diet-driven weight gain

    Journal: Nature Communications

    doi: 10.1038/s41467-021-23084-1

    APRIL-deficient mice are protected from obesity development. a – j WT and APRIL −/− mice were fed HFD for 20 weeks. a Systemic BAFF concentration quantified by ELISA. b Weight gain. c Tissue weight distribution as indicated at time of harvest. d Food Intake. e Combined energy expenditure at 16 weeks of HFD analyzed by TSE Phenomaster system. f Fasting glucose at 20 weeks of HFD. g GTT at 14 weeks of HFD. h AUC. i Fasting insulin at 20 weeks of HFD. j Systemic ALT at 20 weeks of HFD. k Weight gain of WT, BAFF −/− , APRIL −/− , or BAFF −/− /APRIL −/− mice fed HFD for 20 weeks. a – j Representative of three independent experiments, n = 6-7/condition. a – j For bar and line graphs, data represent mean ± SEM. a , c – f , i – j Unpaired two-tailed Student’s t test. * p
    Figure Legend Snippet: APRIL-deficient mice are protected from obesity development. a – j WT and APRIL −/− mice were fed HFD for 20 weeks. a Systemic BAFF concentration quantified by ELISA. b Weight gain. c Tissue weight distribution as indicated at time of harvest. d Food Intake. e Combined energy expenditure at 16 weeks of HFD analyzed by TSE Phenomaster system. f Fasting glucose at 20 weeks of HFD. g GTT at 14 weeks of HFD. h AUC. i Fasting insulin at 20 weeks of HFD. j Systemic ALT at 20 weeks of HFD. k Weight gain of WT, BAFF −/− , APRIL −/− , or BAFF −/− /APRIL −/− mice fed HFD for 20 weeks. a – j Representative of three independent experiments, n = 6-7/condition. a – j For bar and line graphs, data represent mean ± SEM. a , c – f , i – j Unpaired two-tailed Student’s t test. * p

    Techniques Used: Mouse Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test

    BAFF axis regulates obesity development. a Weight gain of WT or RP105 −/− mice fed a high-fat diet (HFD) for 24 weeks. b – d WT, RP105 −/− or RP105 −/− /BAFF −/− mice were fed HFD for 16 weeks. b Weight gain. c Fasting glucose. d Glucose tolerance test (GTT). e Area under the curve (AUC). f Low-fat chow diet fed WT, RP105 −/− , or RP105 −/− /BAFF −/− were monitored in TSE Phenomaster systems for 3 days. Combined energy expenditure. g – h WT, RP105 −/− , μMT or BAFF-Tg mice fed HFD for 15 weeks. g Systemic BAFF concentration quantified by ELISA. h Weight gain. i – m WT or BAFF-Tg mice fed HFD for 20 weeks. i Fasting glucose at 20 weeks. j GTT at 12 weeks. k AUC. l Liver triglycerides quantified by colorimetric assay at harvest. m Systemic alanine transaminase (ALT) at harvest. a Representative of six independent experiments, n = 4–8/condition. b – e Representative of three independent experiments, n = 4–7/condition. f A single experiment, n = 5–6/condition. g – h Representative of three independent experiments, n = 3–4/condition. i – m Representative of three independent experiments, n = 3–6/condition. a – m For bar and line graphs, data represents mean±SEM. a – b , d – e , j – k Area under the curve. N.S = not significant, * p
    Figure Legend Snippet: BAFF axis regulates obesity development. a Weight gain of WT or RP105 −/− mice fed a high-fat diet (HFD) for 24 weeks. b – d WT, RP105 −/− or RP105 −/− /BAFF −/− mice were fed HFD for 16 weeks. b Weight gain. c Fasting glucose. d Glucose tolerance test (GTT). e Area under the curve (AUC). f Low-fat chow diet fed WT, RP105 −/− , or RP105 −/− /BAFF −/− were monitored in TSE Phenomaster systems for 3 days. Combined energy expenditure. g – h WT, RP105 −/− , μMT or BAFF-Tg mice fed HFD for 15 weeks. g Systemic BAFF concentration quantified by ELISA. h Weight gain. i – m WT or BAFF-Tg mice fed HFD for 20 weeks. i Fasting glucose at 20 weeks. j GTT at 12 weeks. k AUC. l Liver triglycerides quantified by colorimetric assay at harvest. m Systemic alanine transaminase (ALT) at harvest. a Representative of six independent experiments, n = 4–8/condition. b – e Representative of three independent experiments, n = 4–7/condition. f A single experiment, n = 5–6/condition. g – h Representative of three independent experiments, n = 3–4/condition. i – m Representative of three independent experiments, n = 3–6/condition. a – m For bar and line graphs, data represents mean±SEM. a – b , d – e , j – k Area under the curve. N.S = not significant, * p

    Techniques Used: Mouse Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Colorimetric Assay

    BAFF modifies brown adipose adaptive thermogenesis. a – b WT, RP105 −/− , or BAFF-Tg mice were fed HFD for 20 weeks. a BAT Ucp1 mRNA expression quantified by qPCR. b BAT mitochondria oxygen consumption. c – d Brown adipocytes were treated with saline (NS) or rBAFF (500 ng/ml) for 6 hours. c Ucp1 mRNA expression quantified by qPCR, relative expression to NS. d Oxygen consumption rate (OCR). e Oxygen consumption of WT mice treated with saline (NS) or rBAFF (2 μg/mouse) for 24 hours prior to norepinephrine (NE; 1 mg/kg) challenge. f – g Obese WT mice treated with rBAFF (2 μg/mouse) every other day for 1 week and monitored in TSE Phenomaster. f Monitoring of energy expenditure over 5 days. g Bar graph of combined energy expenditure. a – b Representative of n = 3–6/condition. c – d Representative of three independent experiments, n = 4–5/condition. e Representative of two independent experiments, n = 3–4/condition. f – g A single experiment, n = 3–5/condition. a – g For bar and line graphs, data represents mean±SEM. a – d Unpaired two-tailed Student’s t test. e Area under the curve. f – g analysis of covariance (ANCOVA) with body weight as covariate. * p
    Figure Legend Snippet: BAFF modifies brown adipose adaptive thermogenesis. a – b WT, RP105 −/− , or BAFF-Tg mice were fed HFD for 20 weeks. a BAT Ucp1 mRNA expression quantified by qPCR. b BAT mitochondria oxygen consumption. c – d Brown adipocytes were treated with saline (NS) or rBAFF (500 ng/ml) for 6 hours. c Ucp1 mRNA expression quantified by qPCR, relative expression to NS. d Oxygen consumption rate (OCR). e Oxygen consumption of WT mice treated with saline (NS) or rBAFF (2 μg/mouse) for 24 hours prior to norepinephrine (NE; 1 mg/kg) challenge. f – g Obese WT mice treated with rBAFF (2 μg/mouse) every other day for 1 week and monitored in TSE Phenomaster. f Monitoring of energy expenditure over 5 days. g Bar graph of combined energy expenditure. a – b Representative of n = 3–6/condition. c – d Representative of three independent experiments, n = 4–5/condition. e Representative of two independent experiments, n = 3–4/condition. f – g A single experiment, n = 3–5/condition. a – g For bar and line graphs, data represents mean±SEM. a – d Unpaired two-tailed Student’s t test. e Area under the curve. f – g analysis of covariance (ANCOVA) with body weight as covariate. * p

    Techniques Used: Mouse Assay, Expressing, Real-time Polymerase Chain Reaction, Two Tailed Test

    3) Product Images from "Reassessment of the involvement of Snord115 in the serotonin 2c receptor pathway in a genetically relevant mouse model"

    Article Title: Reassessment of the involvement of Snord115 in the serotonin 2c receptor pathway in a genetically relevant mouse model

    Journal: eLife

    doi: 10.7554/eLife.60862

    Snord115 -deficient mice show regular homeotic feeding behavior and energy balance. Body weight ( A ) and body composition measured by EchoMRI ( B ). Glucose tolerance test (GTT) of overnight (16 hr) fasted Snord115 -deficient mice (n=14) and their WT littermates (n=9) (black and white squares, respectively). The graph represents the fate of glucose versus time after i.p. glucose administration ( C ). Food intake ( D ), spontaneous locomotion ( E ), energy expenditure ( F ), and respiratory exchange ratio ( G ) of Snord115 -deficient mice and WT littermates were measured using the TSE PhenoMaster System. ( H-K ): Snord115 -deficient mice and WT littermates were overnight (18 hr) starved. Expression levels of Pomc, Agrp and Npy mRNAs in hypothalamus were measured (RT-qPCR relative to Gapdh ) ( H ). Weight loss after fasting ( I ), blood glucose ( J ) and ketone bodies ( K ) levels were measured in the fed and starved states. ( L ) Heatmap of the normalized mRNA-seq read counts from a list of genes of interest, as indicated below the panel. The normalized expression for each gene (column) and each genotype (row) is represented by colour code (yellow, lower value; red, higher value). Note that Gm24966 and Gm26498 match the deleted region. ( M ) Snord115 -deficient mice and WT littermates were overnight (16 hr) starved and food intake of mCPP- and NaCl-treated mice was recorded using the TSE Phenomaster system during the first and second hour after treatment. mCPP was i.p. administered (1 mg/kg, M-top) or (5 mg/kg, M-bottom). White and black bars represent WT and Snord115 -deficient mice, respectively. The number of individuals analysed are indicated within histograms. None of the analyses in panels A-K and M showed a significant effect of genotype (ANOVA p-values > 0.05 for genotype in each panel).
    Figure Legend Snippet: Snord115 -deficient mice show regular homeotic feeding behavior and energy balance. Body weight ( A ) and body composition measured by EchoMRI ( B ). Glucose tolerance test (GTT) of overnight (16 hr) fasted Snord115 -deficient mice (n=14) and their WT littermates (n=9) (black and white squares, respectively). The graph represents the fate of glucose versus time after i.p. glucose administration ( C ). Food intake ( D ), spontaneous locomotion ( E ), energy expenditure ( F ), and respiratory exchange ratio ( G ) of Snord115 -deficient mice and WT littermates were measured using the TSE PhenoMaster System. ( H-K ): Snord115 -deficient mice and WT littermates were overnight (18 hr) starved. Expression levels of Pomc, Agrp and Npy mRNAs in hypothalamus were measured (RT-qPCR relative to Gapdh ) ( H ). Weight loss after fasting ( I ), blood glucose ( J ) and ketone bodies ( K ) levels were measured in the fed and starved states. ( L ) Heatmap of the normalized mRNA-seq read counts from a list of genes of interest, as indicated below the panel. The normalized expression for each gene (column) and each genotype (row) is represented by colour code (yellow, lower value; red, higher value). Note that Gm24966 and Gm26498 match the deleted region. ( M ) Snord115 -deficient mice and WT littermates were overnight (16 hr) starved and food intake of mCPP- and NaCl-treated mice was recorded using the TSE Phenomaster system during the first and second hour after treatment. mCPP was i.p. administered (1 mg/kg, M-top) or (5 mg/kg, M-bottom). White and black bars represent WT and Snord115 -deficient mice, respectively. The number of individuals analysed are indicated within histograms. None of the analyses in panels A-K and M showed a significant effect of genotype (ANOVA p-values > 0.05 for genotype in each panel).

    Techniques Used: Mouse Assay, Expressing, Quantitative RT-PCR

    4) Product Images from "Testing Effects of Chronic Chemogenetic Neuronal Stimulation on Energy Balance by Indirect Calorimetry"

    Article Title: Testing Effects of Chronic Chemogenetic Neuronal Stimulation on Energy Balance by Indirect Calorimetry

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.2811

    A mouse housing box for the PhenoMaster TSE system Each box is equipped with tubing for gas in and out, weight sensors for water and food, and an infrared light beam frame for locomotor activity.
    Figure Legend Snippet: A mouse housing box for the PhenoMaster TSE system Each box is equipped with tubing for gas in and out, weight sensors for water and food, and an infrared light beam frame for locomotor activity.

    Techniques Used: Activity Assay

    The PhenoMaster TSE system One climate chamber can harbor 6 boxes that can measure O 2 , CO 2 , food intake, water consumption.
    Figure Legend Snippet: The PhenoMaster TSE system One climate chamber can harbor 6 boxes that can measure O 2 , CO 2 , food intake, water consumption.

    Techniques Used:

    5) Product Images from "Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice "

    Article Title: Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice

    Journal: Behavioural Brain Research

    doi: 10.1016/j.bbr.2013.04.041

    Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the PhenoMaster ( Fig. 3 D). * p
    Figure Legend Snippet: Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the PhenoMaster ( Fig. 3 D). * p

    Techniques Used: Activity Assay, Mouse Assay

    Habituation of Mecp2 Stop and WT mice to novel home cages recorded by the PhenoMaster. Animals were recorded upon introduction to the novel cage in 10-min bins for 3 h (habituation complete in WT mice). Mean ± SEM. (A) Activity profiling confirmed higher activity in WT during early phases of habituation (asterisks are for 10–30 min, p
    Figure Legend Snippet: Habituation of Mecp2 Stop and WT mice to novel home cages recorded by the PhenoMaster. Animals were recorded upon introduction to the novel cage in 10-min bins for 3 h (habituation complete in WT mice). Mean ± SEM. (A) Activity profiling confirmed higher activity in WT during early phases of habituation (asterisks are for 10–30 min, p

    Techniques Used: Mouse Assay, Activity Assay

    Home cage activity as recorded by PhenoMaster. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active during the dark phase with a strong locomotor deficit apparent at 2–6 h of the dark phase. Indeed, nocturnal activity was significantly higher in WT mice (Bi), but not during light phases (Bii), and was due to reductions in both ambulatory (Ci) and fine movements (Cii). Automatically monitored food intake was higher in Mecp2 Stop mice on all recording days (Di) and increased cumulative food intake over 3 days was due to nocturnal eating (Dii). * p
    Figure Legend Snippet: Home cage activity as recorded by PhenoMaster. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active during the dark phase with a strong locomotor deficit apparent at 2–6 h of the dark phase. Indeed, nocturnal activity was significantly higher in WT mice (Bi), but not during light phases (Bii), and was due to reductions in both ambulatory (Ci) and fine movements (Cii). Automatically monitored food intake was higher in Mecp2 Stop mice on all recording days (Di) and increased cumulative food intake over 3 days was due to nocturnal eating (Dii). * p

    Techniques Used: Activity Assay, Mouse Assay

    6) Product Images from "Adipose Tissue is a Critical Regulator of Osteoarthritis"

    Article Title: Adipose Tissue is a Critical Regulator of Osteoarthritis

    Journal: bioRxiv

    doi: 10.1101/2020.06.04.134601

    Timeline for study. First, male and female LD mice and WT littermate controls were fed a chow-control diet from weaning (4 weeks of age) and were challenged with DMM surgery at 16 weeks of age. Metabolic profiles were determined using insulin tolerance tests (ITT) and glucose tolerance tests (GTT) at 20 weeks of age, 4-weeks post DMM. At 22-24 weeks of age, metabolic testing using indirect calorimetry assessments were completed using a Phenomaster device. At 27-weeks of age, one week prior to sacrifice, body composition and behavior assays were completed. Animals were euthanized at 28-weeks of age, 12-weeks post DMM surgery. Tissues were preserved for joint histology and bone microstructure, and serum, synovial fluid, and fecal matter was stored at −80C for profiling. The same timeline was followed for male and female LD and WT HFD-fed mice, with the exception of indirect calorimetry metabolic testing – these animals were not evaluated using the Phenomaster. In the transplant groups, LD host mice either received wild type fat rescue at 6-8 weeks of age, or a cell-based injection of preadipocyte mouse embryonic fibroblasts at 3-5 weeks of age. Both transplant groups were maintained on chow diets, and followed the same protocol as the chow-fed LD and WT mice. The same euthanization and tissue preparation procedures were employed to all groups of mice at 28-weeks of age.
    Figure Legend Snippet: Timeline for study. First, male and female LD mice and WT littermate controls were fed a chow-control diet from weaning (4 weeks of age) and were challenged with DMM surgery at 16 weeks of age. Metabolic profiles were determined using insulin tolerance tests (ITT) and glucose tolerance tests (GTT) at 20 weeks of age, 4-weeks post DMM. At 22-24 weeks of age, metabolic testing using indirect calorimetry assessments were completed using a Phenomaster device. At 27-weeks of age, one week prior to sacrifice, body composition and behavior assays were completed. Animals were euthanized at 28-weeks of age, 12-weeks post DMM surgery. Tissues were preserved for joint histology and bone microstructure, and serum, synovial fluid, and fecal matter was stored at −80C for profiling. The same timeline was followed for male and female LD and WT HFD-fed mice, with the exception of indirect calorimetry metabolic testing – these animals were not evaluated using the Phenomaster. In the transplant groups, LD host mice either received wild type fat rescue at 6-8 weeks of age, or a cell-based injection of preadipocyte mouse embryonic fibroblasts at 3-5 weeks of age. Both transplant groups were maintained on chow diets, and followed the same protocol as the chow-fed LD and WT mice. The same euthanization and tissue preparation procedures were employed to all groups of mice at 28-weeks of age.

    Techniques Used: Mouse Assay, Injection

    Related Articles

    other:

    Article Title: Heterozygous Nme7 Mutation Affects Glucose Tolerance in Male Rats
    Article Snippet: One week later, indirect calorimetry measurement was performed using TSE Phenomaster system.

    Activity Assay:

    Article Title: Dnmt3b Deficiency in Myf5+-Brown Fat Precursor Cells Promotes Obesity in Female Mice
    Article Snippet: .. A PhenoMaster metabolic cage system (TSE Systems, Chesterfield, MO, USA) was used to measure oxygen consumption, carbon dioxide production, respiratory exchange ratio, locomotor activity and food/drink intake. ..

    Article Title: Dnmt3b Deficiency in Myf5+-Brown Fat Precursor Cells Promotes Obesity in Female Mice
    Article Snippet: .. We used a PhenoMaster metabolic cage system to characterize the energy metabolism and found that the female 3bKO mice exhibited lower oxygen consumption and energy expenditure ( D,E), which may largely account for increased body weight and adiposity in these female mice, as there were no differences in respiratory exchange ratio (RER), locomotor activity and food intake between the 3bKO and their fl/fl littermate control mice ( ). ..

    Article Title: Effects of Tryptophan Supplementation and Exercise on the Fate of Kynurenine Metabolites in Mice and Humans
    Article Snippet: .. Oxygen consumption and carbon dioxide production rate measurements as well as food and water intake were recorded every 3 min. Activity was assessed by beam breaks in TSE Phenomaster systems. ..

    Mouse Assay:

    Article Title: Dnmt3b Deficiency in Myf5+-Brown Fat Precursor Cells Promotes Obesity in Female Mice
    Article Snippet: .. We used a PhenoMaster metabolic cage system to characterize the energy metabolism and found that the female 3bKO mice exhibited lower oxygen consumption and energy expenditure ( D,E), which may largely account for increased body weight and adiposity in these female mice, as there were no differences in respiratory exchange ratio (RER), locomotor activity and food intake between the 3bKO and their fl/fl littermate control mice ( ). ..

    Article Title: Effects of Tryptophan Supplementation and Exercise on the Fate of Kynurenine Metabolites in Mice and Humans
    Article Snippet: .. At week 6 of diet, mice were individually housed in the TSE PhenoMaster home cage system (TSE Systems; Bad Homburg Germany) for 5 days in a controlled environment (12 h light/12 h dark, 24–25 °C, 45% humidity) with ad libitum access to food and water except for a 12 h fasting period during the last dark cycle. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TSE systems phenomaster
    Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the <t>PhenoMaster</t> ( Fig. 3 D). * p
    Phenomaster, supplied by TSE systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phenomaster/product/TSE systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phenomaster - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the PhenoMaster ( Fig. 3 D). * p

    Journal: Behavioural Brain Research

    Article Title: Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice

    doi: 10.1016/j.bbr.2013.04.041

    Figure Lengend Snippet: Home cage activity as recorded by PhenoTyper. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active relative to WT during the dark hours with a strong locomotor deficit apparent at 2–5 h of the dark phase. Nocturnal activity was significantly higher in WT mice (Bi), but not diurnal locomotion (Bii). Locomotion of WT mice was concentrated towards the edges of their home cage (patrolling zone: grey shaded area) in which they performed more patrolling (Ci), while Mecp2 Stop mice spent less time in this zone. This was not related to lower ambulatory activity since both WT and Mecp2 Stop mice entered the zone with the same frequency (Cii). A reliable difference was also evident for time spent in food zone (shaded area: in front of food hopper) (D) in keeping with food intake data from the PhenoMaster ( Fig. 3 D). * p

    Article Snippet: Fifty female mice bred at a commercial vendor (Harlan, UK) and delivered to our facility aged 6 months were used and assigned to two cohorts: Cohort 1: n = 30 (13 Mecp2 Stop and 17 WT) were tested in open-field and elevated plus maze; Cohort 2: n = 20 (10 Mecp2 stop and 10 WT) were scanned over 7 days in the PhenoTyper (Noldus IT, Wageningen, The Netherlands) and then over a similar time course in the PhenoMaster (TSE Systems, Bad Homburg, Germany) for circadian activity and food/water intake.

    Techniques: Activity Assay, Mouse Assay

    Habituation of Mecp2 Stop and WT mice to novel home cages recorded by the PhenoMaster. Animals were recorded upon introduction to the novel cage in 10-min bins for 3 h (habituation complete in WT mice). Mean ± SEM. (A) Activity profiling confirmed higher activity in WT during early phases of habituation (asterisks are for 10–30 min, p

    Journal: Behavioural Brain Research

    Article Title: Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice

    doi: 10.1016/j.bbr.2013.04.041

    Figure Lengend Snippet: Habituation of Mecp2 Stop and WT mice to novel home cages recorded by the PhenoMaster. Animals were recorded upon introduction to the novel cage in 10-min bins for 3 h (habituation complete in WT mice). Mean ± SEM. (A) Activity profiling confirmed higher activity in WT during early phases of habituation (asterisks are for 10–30 min, p

    Article Snippet: Fifty female mice bred at a commercial vendor (Harlan, UK) and delivered to our facility aged 6 months were used and assigned to two cohorts: Cohort 1: n = 30 (13 Mecp2 Stop and 17 WT) were tested in open-field and elevated plus maze; Cohort 2: n = 20 (10 Mecp2 stop and 10 WT) were scanned over 7 days in the PhenoTyper (Noldus IT, Wageningen, The Netherlands) and then over a similar time course in the PhenoMaster (TSE Systems, Bad Homburg, Germany) for circadian activity and food/water intake.

    Techniques: Mouse Assay, Activity Assay

    Home cage activity as recorded by PhenoMaster. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active during the dark phase with a strong locomotor deficit apparent at 2–6 h of the dark phase. Indeed, nocturnal activity was significantly higher in WT mice (Bi), but not during light phases (Bii), and was due to reductions in both ambulatory (Ci) and fine movements (Cii). Automatically monitored food intake was higher in Mecp2 Stop mice on all recording days (Di) and increased cumulative food intake over 3 days was due to nocturnal eating (Dii). * p

    Journal: Behavioural Brain Research

    Article Title: Long-term home cage activity scans reveal lowered exploratory behaviour in symptomatic female Rett mice

    doi: 10.1016/j.bbr.2013.04.041

    Figure Lengend Snippet: Home cage activity as recorded by PhenoMaster. Means ± SEM. (A) Circadian activity over a 24-h period (shaded area indicates dark hours, 19:00–07:00). Mecp2 Stop mice were less active during the dark phase with a strong locomotor deficit apparent at 2–6 h of the dark phase. Indeed, nocturnal activity was significantly higher in WT mice (Bi), but not during light phases (Bii), and was due to reductions in both ambulatory (Ci) and fine movements (Cii). Automatically monitored food intake was higher in Mecp2 Stop mice on all recording days (Di) and increased cumulative food intake over 3 days was due to nocturnal eating (Dii). * p

    Article Snippet: Fifty female mice bred at a commercial vendor (Harlan, UK) and delivered to our facility aged 6 months were used and assigned to two cohorts: Cohort 1: n = 30 (13 Mecp2 Stop and 17 WT) were tested in open-field and elevated plus maze; Cohort 2: n = 20 (10 Mecp2 stop and 10 WT) were scanned over 7 days in the PhenoTyper (Noldus IT, Wageningen, The Netherlands) and then over a similar time course in the PhenoMaster (TSE Systems, Bad Homburg, Germany) for circadian activity and food/water intake.

    Techniques: Activity Assay, Mouse Assay