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Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor <t>p53</t> <t>expression</t> levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.
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Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor <t>p53</t> <t>expression</t> levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.
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Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor <t>p53</t> <t>expression</t> levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.
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Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor <t>p53</t> <t>expression</t> levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.
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Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor <t>p53</t> <t>expression</t> levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.
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Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor p53 expression levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.

Journal: International Journal of Cell Biology

Article Title: Casein Kinase I γ 2 Impairs Fibroblasts Actin Stress Fibers Formation and Delays Cell Cycle Progression in G1

doi: 10.1155/2012/684684

Figure Lengend Snippet: Effect of CKI γ 2 overexpression on the CDK inhibitors p21 Cip1 and p27 Kip1 and the tumor suppressor p53 expression levels. (a) From indicated cell lines, equivalent amount of total cell lysate proteins was subjected to Western Blot analysis using specific antibodies against p21 Cip1 , p27 Kip1 , and p53. Nck Western Blot was used as loading control. (b) Similar samples were probed by western blot with anti-HA antibodies.

Article Snippet: For western blot analysis, the following antibodies were used: HA Y-11 (Santa Cruz); p53 FL-393 (Santa Cruz), Nck 1794 (in house [ ]), p21 Cip1 C-10 (Santa Cruz); p27 Waf1 C-19 (Santa Cruz) and RhoA F-1 (Santa Cruz).

Techniques: Over Expression, Expressing, Western Blot