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    ATCC wild type 3 isp479c
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    wild type 3 isp479c  (ATCC)


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    ATCC wild type 3 isp479c
    Wild Type 3 Isp479c, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wild type 3 isp479c  (ATCC)


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    ATCC wild type 3 isp479c
    Strains and plasmids used in this study
    Wild Type 3 Isp479c, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure"

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00630-09

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Clone Assay

    SDS-PAGE analysis (with total protein extracts) of S. aureus strains Newman, 6850, COL, and ISP479C (A) and Newman versus Newman Δsae (B). (A) Changes in the total protein patterns of S. aureus strains Newman, 6850, COL, and ISP479C after treatment with Perform. Generally, a large number of proteins were downregulated, except in strain Newman. (B) Changes in the protein pattern of S. aureus strain Newman after treatment with Perform are dependent on the sae system. The upregulated proteins in SDS-PAGE were analyzed by mass spectrometry (Eap, extracellular adherence protein; LukFS, leukocidin FS; γ-toxin, γ-hemolysin component C; LukE, leukocidin E; ET, putative staphylococcal enterotoxin; Efb, extracellular fibrinogen-binding protein). Total staphylococcal protein extracts were separated by SDS-PAGE (12%). WT, wild type; C, control; P, 0.04% Perform.
    Figure Legend Snippet: SDS-PAGE analysis (with total protein extracts) of S. aureus strains Newman, 6850, COL, and ISP479C (A) and Newman versus Newman Δsae (B). (A) Changes in the total protein patterns of S. aureus strains Newman, 6850, COL, and ISP479C after treatment with Perform. Generally, a large number of proteins were downregulated, except in strain Newman. (B) Changes in the protein pattern of S. aureus strain Newman after treatment with Perform are dependent on the sae system. The upregulated proteins in SDS-PAGE were analyzed by mass spectrometry (Eap, extracellular adherence protein; LukFS, leukocidin FS; γ-toxin, γ-hemolysin component C; LukE, leukocidin E; ET, putative staphylococcal enterotoxin; Efb, extracellular fibrinogen-binding protein). Total staphylococcal protein extracts were separated by SDS-PAGE (12%). WT, wild type; C, control; P, 0.04% Perform.

    Techniques Used: SDS Page, Mass Spectrometry, Binding Assay

    Invasiveness of S. aureus strains Newman, 6850, COL, and ISP479C for 293 cells. (A) Unlike that of other staphylococcal wild-type strains, the invasiveness of S. aureus strain Newman grown in Perform and SDS containing medium increased. The results are means plus SEMs from at least three independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was determined. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on Eap but independent of FnBPs. The results are means plus SEMs from 13 (wild type [WT]), 5 (Δeap), and 3 (ΔfnbAB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.
    Figure Legend Snippet: Invasiveness of S. aureus strains Newman, 6850, COL, and ISP479C for 293 cells. (A) Unlike that of other staphylococcal wild-type strains, the invasiveness of S. aureus strain Newman grown in Perform and SDS containing medium increased. The results are means plus SEMs from at least three independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was determined. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on Eap but independent of FnBPs. The results are means plus SEMs from 13 (wild type [WT]), 5 (Δeap), and 3 (ΔfnbAB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.

    Techniques Used:

    SaeS alignment and invasiveness of S. aureus strains Newman and ISP479C for 293 cells. (A) Only strain Newman harbors a mutation in SaeS. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on the sae system. The results are means plus SEMs from 13 (wild type [WT]), 4 (Δsae), and 3 (sae33) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. (C) Increased invasiveness of S. aureus strain ISP479C after treatment with Perform and SDS is dependent on the Newman sae system. The results are means plus SEMs from four independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. Inset, SDS-PAGE for allele swapping of sae28 (saePQRSISP479C) and sae33 (saePQRSNewman) in strain ISP479C. Eap expression is most prominently increased, paralleling enhanced cellular invasiveness. (D) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is independent of agr and sarA. A sigB knockout seems to further enhance the invasion. The results are means plus SEMs from 13 (WT), 3 (Δagr), 3 (ΔsarA), and 3 (ΔsigB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.
    Figure Legend Snippet: SaeS alignment and invasiveness of S. aureus strains Newman and ISP479C for 293 cells. (A) Only strain Newman harbors a mutation in SaeS. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on the sae system. The results are means plus SEMs from 13 (wild type [WT]), 4 (Δsae), and 3 (sae33) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. (C) Increased invasiveness of S. aureus strain ISP479C after treatment with Perform and SDS is dependent on the Newman sae system. The results are means plus SEMs from four independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. Inset, SDS-PAGE for allele swapping of sae28 (saePQRSISP479C) and sae33 (saePQRSNewman) in strain ISP479C. Eap expression is most prominently increased, paralleling enhanced cellular invasiveness. (D) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is independent of agr and sarA. A sigB knockout seems to further enhance the invasion. The results are means plus SEMs from 13 (WT), 3 (Δagr), 3 (ΔsarA), and 3 (ΔsigB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.

    Techniques Used: Mutagenesis, SDS Page, Expressing, Knock-Out

    wild type 3 isp479c  (ATCC)


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    ATCC wild type 3 isp479c
    Strains and plasmids used in this study
    Wild Type 3 Isp479c, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wild type 3 isp479c/product/ATCC
    Average 90 stars, based on 1 article reviews
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    Images

    1) Product Images from "A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure "

    Article Title: A Point Mutation in the Sensor Histidine Kinase SaeS of Staphylococcus aureus Strain Newman Alters the Response to Biocide Exposure

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00630-09

    Strains and plasmids used in this study
    Figure Legend Snippet: Strains and plasmids used in this study

    Techniques Used: Plasmid Preparation, Clone Assay

    SDS-PAGE analysis (with total protein extracts) of S. aureus strains Newman, 6850, COL, and ISP479C (A) and Newman versus Newman Δsae (B). (A) Changes in the total protein patterns of S. aureus strains Newman, 6850, COL, and ISP479C after treatment with Perform. Generally, a large number of proteins were downregulated, except in strain Newman. (B) Changes in the protein pattern of S. aureus strain Newman after treatment with Perform are dependent on the sae system. The upregulated proteins in SDS-PAGE were analyzed by mass spectrometry (Eap, extracellular adherence protein; LukFS, leukocidin FS; γ-toxin, γ-hemolysin component C; LukE, leukocidin E; ET, putative staphylococcal enterotoxin; Efb, extracellular fibrinogen-binding protein). Total staphylococcal protein extracts were separated by SDS-PAGE (12%). WT, wild type; C, control; P, 0.04% Perform.
    Figure Legend Snippet: SDS-PAGE analysis (with total protein extracts) of S. aureus strains Newman, 6850, COL, and ISP479C (A) and Newman versus Newman Δsae (B). (A) Changes in the total protein patterns of S. aureus strains Newman, 6850, COL, and ISP479C after treatment with Perform. Generally, a large number of proteins were downregulated, except in strain Newman. (B) Changes in the protein pattern of S. aureus strain Newman after treatment with Perform are dependent on the sae system. The upregulated proteins in SDS-PAGE were analyzed by mass spectrometry (Eap, extracellular adherence protein; LukFS, leukocidin FS; γ-toxin, γ-hemolysin component C; LukE, leukocidin E; ET, putative staphylococcal enterotoxin; Efb, extracellular fibrinogen-binding protein). Total staphylococcal protein extracts were separated by SDS-PAGE (12%). WT, wild type; C, control; P, 0.04% Perform.

    Techniques Used: SDS Page, Mass Spectrometry, Binding Assay

    Invasiveness of S. aureus strains Newman, 6850, COL, and ISP479C for 293 cells. (A) Unlike that of other staphylococcal wild-type strains, the invasiveness of S. aureus strain Newman grown in Perform and SDS containing medium increased. The results are means plus SEMs from at least three independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was determined. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on Eap but independent of FnBPs. The results are means plus SEMs from 13 (wild type [WT]), 5 (Δeap), and 3 (ΔfnbAB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.
    Figure Legend Snippet: Invasiveness of S. aureus strains Newman, 6850, COL, and ISP479C for 293 cells. (A) Unlike that of other staphylococcal wild-type strains, the invasiveness of S. aureus strain Newman grown in Perform and SDS containing medium increased. The results are means plus SEMs from at least three independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was determined. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on Eap but independent of FnBPs. The results are means plus SEMs from 13 (wild type [WT]), 5 (Δeap), and 3 (ΔfnbAB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.

    Techniques Used:

    SaeS alignment and invasiveness of S. aureus strains Newman and ISP479C for 293 cells. (A) Only strain Newman harbors a mutation in SaeS. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on the sae system. The results are means plus SEMs from 13 (wild type [WT]), 4 (Δsae), and 3 (sae33) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. (C) Increased invasiveness of S. aureus strain ISP479C after treatment with Perform and SDS is dependent on the Newman sae system. The results are means plus SEMs from four independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. Inset, SDS-PAGE for allele swapping of sae28 (saePQRSISP479C) and sae33 (saePQRSNewman) in strain ISP479C. Eap expression is most prominently increased, paralleling enhanced cellular invasiveness. (D) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is independent of agr and sarA. A sigB knockout seems to further enhance the invasion. The results are means plus SEMs from 13 (WT), 3 (Δagr), 3 (ΔsarA), and 3 (ΔsigB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.
    Figure Legend Snippet: SaeS alignment and invasiveness of S. aureus strains Newman and ISP479C for 293 cells. (A) Only strain Newman harbors a mutation in SaeS. (B) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is dependent on the sae system. The results are means plus SEMs from 13 (wild type [WT]), 4 (Δsae), and 3 (sae33) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. (C) Increased invasiveness of S. aureus strain ISP479C after treatment with Perform and SDS is dependent on the Newman sae system. The results are means plus SEMs from four independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background. Inset, SDS-PAGE for allele swapping of sae28 (saePQRSISP479C) and sae33 (saePQRSNewman) in strain ISP479C. Eap expression is most prominently increased, paralleling enhanced cellular invasiveness. (D) Increased invasiveness of S. aureus strain Newman after treatment with Perform and SDS is independent of agr and sarA. A sigB knockout seems to further enhance the invasion. The results are means plus SEMs from 13 (WT), 3 (Δagr), 3 (ΔsarA), and 3 (ΔsigB) independent experiments run in duplicate and are expressed as relative invasiveness compared to strain Cowan I. The statistical significance of individual conditions in each strain background was in comparison with the respective conditions in the WT background.

    Techniques Used: Mutagenesis, SDS Page, Expressing, Knock-Out

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