melanoma cell line bowes  (ATCC)


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    ATCC melanoma cell line bowes
    The proliferation, zinc content, and metallothionein II A (MT-IIA) expression in human <t>explant</t> <t>melanoma</t> cells, <t>Bowes</t> cell line and normal human melanocytes HEM. Established explant human melanoma cultures (labeled M1–M10), human melanoma cell line Bowes and normal human melanocytes HEM were maintained upon standard laboratory conditions and their proliferation was determined by ( A ) colorimetric WST-1 assay measuring the cleavage of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenases in viable cells and by ( B ) measuring the proportion of S-phase cells via EdU-specific fluorescence. Values represent means ± SD of at least three experiments. ( C ) Total zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM. Zinc content was determined by absorption spectrometry. ( D ) Free (labile) zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM as measured by microfluorometry of the zinc-specific dye Newport Green diacetate. Values represent means ± SD of at least three experiments. ( E ) The expression of metallothionein II-A (MT-IIA) in melanoma cell lysates as determined by immunoblotting analysis. The numbers in the blot image refer to fold increase or decrease in the density of the particular protein compared to the density of the same protein in HEM cells. Shown is one typical result of at least four experiments.
    Melanoma Cell Line Bowes, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/melanoma cell line bowes/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    melanoma cell line bowes - by Bioz Stars, 2023-12
    93/100 stars

    Images

    1) Product Images from "Acute Increases in Intracellular Zinc Lead to an Increased Lysosomal and Mitochondrial Autophagy and Subsequent Cell Demise in Malignant Melanoma"

    Article Title: Acute Increases in Intracellular Zinc Lead to an Increased Lysosomal and Mitochondrial Autophagy and Subsequent Cell Demise in Malignant Melanoma

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22020667

    The proliferation, zinc content, and metallothionein II A (MT-IIA) expression in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. Established explant human melanoma cultures (labeled M1–M10), human melanoma cell line Bowes and normal human melanocytes HEM were maintained upon standard laboratory conditions and their proliferation was determined by ( A ) colorimetric WST-1 assay measuring the cleavage of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenases in viable cells and by ( B ) measuring the proportion of S-phase cells via EdU-specific fluorescence. Values represent means ± SD of at least three experiments. ( C ) Total zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM. Zinc content was determined by absorption spectrometry. ( D ) Free (labile) zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM as measured by microfluorometry of the zinc-specific dye Newport Green diacetate. Values represent means ± SD of at least three experiments. ( E ) The expression of metallothionein II-A (MT-IIA) in melanoma cell lysates as determined by immunoblotting analysis. The numbers in the blot image refer to fold increase or decrease in the density of the particular protein compared to the density of the same protein in HEM cells. Shown is one typical result of at least four experiments.
    Figure Legend Snippet: The proliferation, zinc content, and metallothionein II A (MT-IIA) expression in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. Established explant human melanoma cultures (labeled M1–M10), human melanoma cell line Bowes and normal human melanocytes HEM were maintained upon standard laboratory conditions and their proliferation was determined by ( A ) colorimetric WST-1 assay measuring the cleavage of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenases in viable cells and by ( B ) measuring the proportion of S-phase cells via EdU-specific fluorescence. Values represent means ± SD of at least three experiments. ( C ) Total zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM. Zinc content was determined by absorption spectrometry. ( D ) Free (labile) zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM as measured by microfluorometry of the zinc-specific dye Newport Green diacetate. Values represent means ± SD of at least three experiments. ( E ) The expression of metallothionein II-A (MT-IIA) in melanoma cell lysates as determined by immunoblotting analysis. The numbers in the blot image refer to fold increase or decrease in the density of the particular protein compared to the density of the same protein in HEM cells. Shown is one typical result of at least four experiments.

    Techniques Used: Expressing, Labeling, WST-1 Assay, Fluorescence, Western Blot

    Autophagic activity in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. ( A ) Autophagic flux (the rate of formation of autophagosomes and autophagolysosomes) in individual cells was determined with help of RFP-GFP-LC3B reporter system following cell transduction and subsequent quantitation of yellow (LC3B positive autophagosomes) and red (LC3B positive autophagolysosome) fluorescence with the determined ratio of yellow/red puncta per cell. ( B , C ) The expression of proteins Beclin-1 and LC3B in the examined cells were determined fluorimetrically. Values represent means ± SD of at least three experiments.
    Figure Legend Snippet: Autophagic activity in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. ( A ) Autophagic flux (the rate of formation of autophagosomes and autophagolysosomes) in individual cells was determined with help of RFP-GFP-LC3B reporter system following cell transduction and subsequent quantitation of yellow (LC3B positive autophagosomes) and red (LC3B positive autophagolysosome) fluorescence with the determined ratio of yellow/red puncta per cell. ( B , C ) The expression of proteins Beclin-1 and LC3B in the examined cells were determined fluorimetrically. Values represent means ± SD of at least three experiments.

    Techniques Used: Activity Assay, Transduction, Quantitation Assay, Fluorescence, Expressing

    melanoma cell line bowes  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
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    • 93
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    Structured Review

    ATCC melanoma cell line bowes
    The proliferation, zinc content, and metallothionein II A (MT-IIA) expression in human <t>explant</t> <t>melanoma</t> cells, <t>Bowes</t> cell line and normal human melanocytes HEM. Established explant human melanoma cultures (labeled M1–M10), human melanoma cell line Bowes and normal human melanocytes HEM were maintained upon standard laboratory conditions and their proliferation was determined by ( A ) colorimetric WST-1 assay measuring the cleavage of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenases in viable cells and by ( B ) measuring the proportion of S-phase cells via EdU-specific fluorescence. Values represent means ± SD of at least three experiments. ( C ) Total zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM. Zinc content was determined by absorption spectrometry. ( D ) Free (labile) zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM as measured by microfluorometry of the zinc-specific dye Newport Green diacetate. Values represent means ± SD of at least three experiments. ( E ) The expression of metallothionein II-A (MT-IIA) in melanoma cell lysates as determined by immunoblotting analysis. The numbers in the blot image refer to fold increase or decrease in the density of the particular protein compared to the density of the same protein in HEM cells. Shown is one typical result of at least four experiments.
    Melanoma Cell Line Bowes, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/melanoma cell line bowes/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    melanoma cell line bowes - by Bioz Stars, 2023-12
    93/100 stars

    Images

    1) Product Images from "Acute Increases in Intracellular Zinc Lead to an Increased Lysosomal and Mitochondrial Autophagy and Subsequent Cell Demise in Malignant Melanoma"

    Article Title: Acute Increases in Intracellular Zinc Lead to an Increased Lysosomal and Mitochondrial Autophagy and Subsequent Cell Demise in Malignant Melanoma

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22020667

    The proliferation, zinc content, and metallothionein II A (MT-IIA) expression in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. Established explant human melanoma cultures (labeled M1–M10), human melanoma cell line Bowes and normal human melanocytes HEM were maintained upon standard laboratory conditions and their proliferation was determined by ( A ) colorimetric WST-1 assay measuring the cleavage of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenases in viable cells and by ( B ) measuring the proportion of S-phase cells via EdU-specific fluorescence. Values represent means ± SD of at least three experiments. ( C ) Total zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM. Zinc content was determined by absorption spectrometry. ( D ) Free (labile) zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM as measured by microfluorometry of the zinc-specific dye Newport Green diacetate. Values represent means ± SD of at least three experiments. ( E ) The expression of metallothionein II-A (MT-IIA) in melanoma cell lysates as determined by immunoblotting analysis. The numbers in the blot image refer to fold increase or decrease in the density of the particular protein compared to the density of the same protein in HEM cells. Shown is one typical result of at least four experiments.
    Figure Legend Snippet: The proliferation, zinc content, and metallothionein II A (MT-IIA) expression in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. Established explant human melanoma cultures (labeled M1–M10), human melanoma cell line Bowes and normal human melanocytes HEM were maintained upon standard laboratory conditions and their proliferation was determined by ( A ) colorimetric WST-1 assay measuring the cleavage of tetrazolium salt WST-1 by mitochondrial succinate dehydrogenases in viable cells and by ( B ) measuring the proportion of S-phase cells via EdU-specific fluorescence. Values represent means ± SD of at least three experiments. ( C ) Total zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM. Zinc content was determined by absorption spectrometry. ( D ) Free (labile) zinc content in human explant melanoma cells (M1–M10), Bowes cell line and normal human melanocytes HEM as measured by microfluorometry of the zinc-specific dye Newport Green diacetate. Values represent means ± SD of at least three experiments. ( E ) The expression of metallothionein II-A (MT-IIA) in melanoma cell lysates as determined by immunoblotting analysis. The numbers in the blot image refer to fold increase or decrease in the density of the particular protein compared to the density of the same protein in HEM cells. Shown is one typical result of at least four experiments.

    Techniques Used: Expressing, Labeling, WST-1 Assay, Fluorescence, Western Blot

    Autophagic activity in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. ( A ) Autophagic flux (the rate of formation of autophagosomes and autophagolysosomes) in individual cells was determined with help of RFP-GFP-LC3B reporter system following cell transduction and subsequent quantitation of yellow (LC3B positive autophagosomes) and red (LC3B positive autophagolysosome) fluorescence with the determined ratio of yellow/red puncta per cell. ( B , C ) The expression of proteins Beclin-1 and LC3B in the examined cells were determined fluorimetrically. Values represent means ± SD of at least three experiments.
    Figure Legend Snippet: Autophagic activity in human explant melanoma cells, Bowes cell line and normal human melanocytes HEM. ( A ) Autophagic flux (the rate of formation of autophagosomes and autophagolysosomes) in individual cells was determined with help of RFP-GFP-LC3B reporter system following cell transduction and subsequent quantitation of yellow (LC3B positive autophagosomes) and red (LC3B positive autophagolysosome) fluorescence with the determined ratio of yellow/red puncta per cell. ( B , C ) The expression of proteins Beclin-1 and LC3B in the examined cells were determined fluorimetrically. Values represent means ± SD of at least three experiments.

    Techniques Used: Activity Assay, Transduction, Quantitation Assay, Fluorescence, Expressing

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