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latimer 2023  (ATCC)


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    ATCC latimer 2023
    Latimer 2023, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/latimer 2023/product/ATCC
    Average 93 stars, based on 1 article reviews
    latimer 2023 - by Bioz Stars, 2025-07
    93/100 stars

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    SLAMF1 expression is increased in MTX-resistant choriocarcinoma cells. ( A ). The mass spectrum of SLAMF1 unique peptide (SLENSVENK) identified by TMT labeling and LC-MS/MS. ( B ). Relative expression of SLAMF1 protein in JEG3 and its MTX-resistant subline JEG3/MTX. *P<0.05. ( C ). Western blotting validation on SLAMF1 expression in MTX-resistant choriocarcinoma sublines and their parental cell lines. β-actin was used as loading control.

    Journal: Cancer Management and Research

    Article Title: SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells

    doi: 10.2147/CMAR.S278012

    Figure Lengend Snippet: SLAMF1 expression is increased in MTX-resistant choriocarcinoma cells. ( A ). The mass spectrum of SLAMF1 unique peptide (SLENSVENK) identified by TMT labeling and LC-MS/MS. ( B ). Relative expression of SLAMF1 protein in JEG3 and its MTX-resistant subline JEG3/MTX. *P<0.05. ( C ). Western blotting validation on SLAMF1 expression in MTX-resistant choriocarcinoma sublines and their parental cell lines. β-actin was used as loading control.

    Article Snippet: Primary antibodies used in this study were provided by the following sources: SLAMF1 (PA5-96046), Invitrogen (Carlsbad, CA, USA); β-actin (#58169), LC3B (#2775), p62 (#8025), and cleaved Caspase-3 (#9664), Cell Signaling (Danvers, MA, USA).

    Techniques: Expressing, Labeling, Liquid Chromatography with Mass Spectroscopy, Western Blot

    Knockdown of SLAMF1 enhanced MTX chemosensitivity in MTX-resistant choriocarcinoma cell lines. ( A ). SLAMF1 expression was markedly attenuated by gene-specific shRNA in JEG3/MTX and JAR/MTX cells. ( B ). Knockdown of SLAMF1 increased MTX sensitivity in JEG3/MTX and JAR/MTX cells. The cell viability in Scr or shSLAMF1 without MTX treatment was regarded as 100%, respectively. n=3,*P<0.01. ( C ). Knockdown of SLAMF1 attenuated the proliferative potential of JEG3/MTX and JAR/MTX cells after MTX treatment for 48 hours. The BrdU incorporation in Scr without MTX treatment was regarded as 100%. n=3, *P<0.05, as compared with Scr without MTX treatment. **P<0.05, as compared with Scr with MTX treatment. ( D ). Knockdown of SLAMF1 impaired soft agar clonogenesis after MTX treatment in JEG3/MTX and JAR/MTX cells. The colony formation in Scr group without MTX treatment was regarded as 100%, respectively. n=3, *P<0.05, as compared with Scr without MTX treatment. **P<0.05, as compared with Scr with MTX treatment.

    Journal: Cancer Management and Research

    Article Title: SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells

    doi: 10.2147/CMAR.S278012

    Figure Lengend Snippet: Knockdown of SLAMF1 enhanced MTX chemosensitivity in MTX-resistant choriocarcinoma cell lines. ( A ). SLAMF1 expression was markedly attenuated by gene-specific shRNA in JEG3/MTX and JAR/MTX cells. ( B ). Knockdown of SLAMF1 increased MTX sensitivity in JEG3/MTX and JAR/MTX cells. The cell viability in Scr or shSLAMF1 without MTX treatment was regarded as 100%, respectively. n=3,*P<0.01. ( C ). Knockdown of SLAMF1 attenuated the proliferative potential of JEG3/MTX and JAR/MTX cells after MTX treatment for 48 hours. The BrdU incorporation in Scr without MTX treatment was regarded as 100%. n=3, *P<0.05, as compared with Scr without MTX treatment. **P<0.05, as compared with Scr with MTX treatment. ( D ). Knockdown of SLAMF1 impaired soft agar clonogenesis after MTX treatment in JEG3/MTX and JAR/MTX cells. The colony formation in Scr group without MTX treatment was regarded as 100%, respectively. n=3, *P<0.05, as compared with Scr without MTX treatment. **P<0.05, as compared with Scr with MTX treatment.

    Article Snippet: Primary antibodies used in this study were provided by the following sources: SLAMF1 (PA5-96046), Invitrogen (Carlsbad, CA, USA); β-actin (#58169), LC3B (#2775), p62 (#8025), and cleaved Caspase-3 (#9664), Cell Signaling (Danvers, MA, USA).

    Techniques: Expressing, shRNA, BrdU Incorporation Assay

    Over-expression of SLAMF1 promoted chemoresistance to MTX in JEG3 and JAR cells. ( A ). SLAMF1 expression was considerably increased in JEG3 and JAR cells. ( B ). Over-expression of SLAMF1 promoted chemoresistance to MTX in JEG3 and JAR cells. n=3, *P<0.05. ( C ). Over-expression of SLAMF1 enhanced the proliferative potential of choriocarcinoma cells after MTX treatment. The BrdU incorporation in EV of each cell line without MTX treatment was regarded as 100%, respectively. n=3, *P<0.05, as compared with EV without MTX treatment; **P<0.05, as compared with EV with MTX treatment. ( D ). Over-expression of SLAMF1 rescued soft agar clonogenesis after MTX treatment in choriocarcinoma cell lines. The colony formation in EV of each cell line without MTX treatment was regarded as 100%, respectively. n=3, *P<0.05, as compared with EV without MTX treatment; **P<0.05, as compared with EV with MTX treatment.

    Journal: Cancer Management and Research

    Article Title: SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells

    doi: 10.2147/CMAR.S278012

    Figure Lengend Snippet: Over-expression of SLAMF1 promoted chemoresistance to MTX in JEG3 and JAR cells. ( A ). SLAMF1 expression was considerably increased in JEG3 and JAR cells. ( B ). Over-expression of SLAMF1 promoted chemoresistance to MTX in JEG3 and JAR cells. n=3, *P<0.05. ( C ). Over-expression of SLAMF1 enhanced the proliferative potential of choriocarcinoma cells after MTX treatment. The BrdU incorporation in EV of each cell line without MTX treatment was regarded as 100%, respectively. n=3, *P<0.05, as compared with EV without MTX treatment; **P<0.05, as compared with EV with MTX treatment. ( D ). Over-expression of SLAMF1 rescued soft agar clonogenesis after MTX treatment in choriocarcinoma cell lines. The colony formation in EV of each cell line without MTX treatment was regarded as 100%, respectively. n=3, *P<0.05, as compared with EV without MTX treatment; **P<0.05, as compared with EV with MTX treatment.

    Article Snippet: Primary antibodies used in this study were provided by the following sources: SLAMF1 (PA5-96046), Invitrogen (Carlsbad, CA, USA); β-actin (#58169), LC3B (#2775), p62 (#8025), and cleaved Caspase-3 (#9664), Cell Signaling (Danvers, MA, USA).

    Techniques: Over Expression, Expressing, BrdU Incorporation Assay

    SLAMF1 regulates autophagy in choriocarcinoma cells. ( A ). Knockdown of SLAMF1 attenuated the expression of autophagy-related genes (LC3-II and p62) in MTX-resistant choriocarcinoma sublines. ( B ). MDC incorporation in MTX-resistant choriocarcinoma sublines with or without SLAMF1 depletion. n=3, ***P<0.001. ( C ). SLAMF1 over-expression increased the expression of autophagy-related genes (LC3-II and p62) in choriocarcinoma cells. β-actin was used as loading control. ( D ). MDC incorporation in choriocarcinoma cell lines with or without SLAMF1 expression. n=3, *P<0.05; **P<0.01.

    Journal: Cancer Management and Research

    Article Title: SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells

    doi: 10.2147/CMAR.S278012

    Figure Lengend Snippet: SLAMF1 regulates autophagy in choriocarcinoma cells. ( A ). Knockdown of SLAMF1 attenuated the expression of autophagy-related genes (LC3-II and p62) in MTX-resistant choriocarcinoma sublines. ( B ). MDC incorporation in MTX-resistant choriocarcinoma sublines with or without SLAMF1 depletion. n=3, ***P<0.001. ( C ). SLAMF1 over-expression increased the expression of autophagy-related genes (LC3-II and p62) in choriocarcinoma cells. β-actin was used as loading control. ( D ). MDC incorporation in choriocarcinoma cell lines with or without SLAMF1 expression. n=3, *P<0.05; **P<0.01.

    Article Snippet: Primary antibodies used in this study were provided by the following sources: SLAMF1 (PA5-96046), Invitrogen (Carlsbad, CA, USA); β-actin (#58169), LC3B (#2775), p62 (#8025), and cleaved Caspase-3 (#9664), Cell Signaling (Danvers, MA, USA).

    Techniques: Expressing, Over Expression

    Depletion of SLAMF1 induced apoptosis in MTX-resistant choriocarcinoma cells treated with MTX. Western blotting analysis detects the expression of LC3-II, p62 and cleaved caspase-3 after MTX treatment in shSLAMF1 or Scr group.

    Journal: Cancer Management and Research

    Article Title: SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells

    doi: 10.2147/CMAR.S278012

    Figure Lengend Snippet: Depletion of SLAMF1 induced apoptosis in MTX-resistant choriocarcinoma cells treated with MTX. Western blotting analysis detects the expression of LC3-II, p62 and cleaved caspase-3 after MTX treatment in shSLAMF1 or Scr group.

    Article Snippet: Primary antibodies used in this study were provided by the following sources: SLAMF1 (PA5-96046), Invitrogen (Carlsbad, CA, USA); β-actin (#58169), LC3B (#2775), p62 (#8025), and cleaved Caspase-3 (#9664), Cell Signaling (Danvers, MA, USA).

    Techniques: Western Blot, Expressing