y pestis 33 83 53 np 404241 1 s6  (ATCC)


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    ATCC y pestis 33 83 53 np 404241 1 s6
    Y Pestis 33 83 53 Np 404241 1 S6, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    single crispr locus  (ATCC)


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    ATCC single crispr locus
    <t>CRISPR</t> adaptation to HHPV-2 infection. ( A ) Depiction of the single CRISPR structure and the preceding cas operon carried by the <t>H.</t> <t>hispanica</t> ATCC 33960 genome. Primers used to examine CRISPR expansion (in panel B) are shown as black arrows and listed in Supplementary Table S2 . ( B ) PCR assay to detect CRISPR expansion at the leader end (L1–L2), the inner part (I1–I2) or the distal end (D1–D2). DNA sampled from infected (+) or uninfected (−) cells was used as PCR templates. Lane M, dsDNA size marker. ( C ) The sequence logo showing the conserved PAM of TTC. The 20 nt upstream of each protospacer observed during HHPV-2 infection were collected and analyzed with WebLogo ( http://weblogo.berkeley.edu/logo.cgi ).
    Single Crispr Locus, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process"

    Article Title: Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkt1154

    CRISPR adaptation to HHPV-2 infection. ( A ) Depiction of the single CRISPR structure and the preceding cas operon carried by the H. hispanica ATCC 33960 genome. Primers used to examine CRISPR expansion (in panel B) are shown as black arrows and listed in Supplementary Table S2 . ( B ) PCR assay to detect CRISPR expansion at the leader end (L1–L2), the inner part (I1–I2) or the distal end (D1–D2). DNA sampled from infected (+) or uninfected (−) cells was used as PCR templates. Lane M, dsDNA size marker. ( C ) The sequence logo showing the conserved PAM of TTC. The 20 nt upstream of each protospacer observed during HHPV-2 infection were collected and analyzed with WebLogo ( http://weblogo.berkeley.edu/logo.cgi ).
    Figure Legend Snippet: CRISPR adaptation to HHPV-2 infection. ( A ) Depiction of the single CRISPR structure and the preceding cas operon carried by the H. hispanica ATCC 33960 genome. Primers used to examine CRISPR expansion (in panel B) are shown as black arrows and listed in Supplementary Table S2 . ( B ) PCR assay to detect CRISPR expansion at the leader end (L1–L2), the inner part (I1–I2) or the distal end (D1–D2). DNA sampled from infected (+) or uninfected (−) cells was used as PCR templates. Lane M, dsDNA size marker. ( C ) The sequence logo showing the conserved PAM of TTC. The 20 nt upstream of each protospacer observed during HHPV-2 infection were collected and analyzed with WebLogo ( http://weblogo.berkeley.edu/logo.cgi ).

    Techniques Used: CRISPR, Infection, Marker, Sequencing

    A preexisting spacer (spacer13) matching the HHPV-2 genome was required. ( A ) Depiction of the imperfect match between spacer13 and an HHPV-2 fragment. Positions of the fragment on the HHPV-2 genome are indicated under the sequence. Three upstream nucleotides corresponding to PAM motif are underlined. ( B ) Depiction of the spacer content of CRISPR variants. Δsp2–6, Δsp7–13, Δsp2–12, Δsp2–13 and Δsp1–14 are designated according to the spacers (or repeat) bordering the omitted region (dashed lines). In Δsp13, spacer13 and the immediately upstream repeat were deleted. ( C ) Adaptation of variant CRISPRs to HHPV-2 infection. Lane M, dsDNA size marker.
    Figure Legend Snippet: A preexisting spacer (spacer13) matching the HHPV-2 genome was required. ( A ) Depiction of the imperfect match between spacer13 and an HHPV-2 fragment. Positions of the fragment on the HHPV-2 genome are indicated under the sequence. Three upstream nucleotides corresponding to PAM motif are underlined. ( B ) Depiction of the spacer content of CRISPR variants. Δsp2–6, Δsp7–13, Δsp2–12, Δsp2–13 and Δsp1–14 are designated according to the spacers (or repeat) bordering the omitted region (dashed lines). In Δsp13, spacer13 and the immediately upstream repeat were deleted. ( C ) Adaptation of variant CRISPRs to HHPV-2 infection. Lane M, dsDNA size marker.

    Techniques Used: Sequencing, CRISPR, Variant Assay, Infection, Marker

    Adaptation to engineered plasmids. ( A ) Adaptation to a modified pWL502 plasmid (pVS) carrying a viral sequence that is partially matched by spacer13. The empty plasmid (pWL502) was used as a control. ( B ) Adaptation to modified pWL502 plasmids carrying different artificial priming protospacers. ( C ) Information of the engineered plasmids in panel B. The plasmid designations indicate their PAM sequences (CTC, TCC or TAC) and different priming protospacers (partially matched by spacer1 or spacer13) on different strands (+ or −). Within the priming protospacer sequence, nucleotides that were designed mismatching the corresponding spacer are shown in bold and underlined. The plus (+) and minus (−) strands correspond, respectively, to the coding and template strands of the pyrF gene. These engineered plasmids were transformed into wild-type DF60 cells and examined for CRISPR expansion. Land Ms, dsDNA size markers.
    Figure Legend Snippet: Adaptation to engineered plasmids. ( A ) Adaptation to a modified pWL502 plasmid (pVS) carrying a viral sequence that is partially matched by spacer13. The empty plasmid (pWL502) was used as a control. ( B ) Adaptation to modified pWL502 plasmids carrying different artificial priming protospacers. ( C ) Information of the engineered plasmids in panel B. The plasmid designations indicate their PAM sequences (CTC, TCC or TAC) and different priming protospacers (partially matched by spacer1 or spacer13) on different strands (+ or −). Within the priming protospacer sequence, nucleotides that were designed mismatching the corresponding spacer are shown in bold and underlined. The plus (+) and minus (−) strands correspond, respectively, to the coding and template strands of the pyrF gene. These engineered plasmids were transformed into wild-type DF60 cells and examined for CRISPR expansion. Land Ms, dsDNA size markers.

    Techniques Used: Modification, Plasmid Preparation, Sequencing, Transformation Assay, CRISPR

    Imperfect matches between preexisting spacers and viral (or phage) DNA. ( A ) S. thermophilus DGCC7710 CRISPR1 (under the GenBank accession number EF434469) contains preexisting spacers matching the Ф858 and Ф2972 genomes. ( B ) Representative matches between haloarchaeal spacers and haloviral genomes. The CRISPR IDs used in the CRISPRdb database ( http://crispr.u-psud.fr/crispr/ ) are given. The match between H. hispanica spacer13 and HHPV-2 is also shown as a reference. The E -value of each match is shown. Dots in the viral (or phage) sequences indicate nucleotides identical to spacers at these positions. The genome positions of the viral (or phage) sequences are indicated by two numbers separated by a colon.
    Figure Legend Snippet: Imperfect matches between preexisting spacers and viral (or phage) DNA. ( A ) S. thermophilus DGCC7710 CRISPR1 (under the GenBank accession number EF434469) contains preexisting spacers matching the Ф858 and Ф2972 genomes. ( B ) Representative matches between haloarchaeal spacers and haloviral genomes. The CRISPR IDs used in the CRISPRdb database ( http://crispr.u-psud.fr/crispr/ ) are given. The match between H. hispanica spacer13 and HHPV-2 is also shown as a reference. The E -value of each match is shown. Dots in the viral (or phage) sequences indicate nucleotides identical to spacers at these positions. The genome positions of the viral (or phage) sequences are indicated by two numbers separated by a colon.

    Techniques Used: CRISPR

    y pestis 33 83 53 np 404241 1 s6  (ATCC)


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    ATCC y pestis 33 83 53 np 404241 1 s6
    Y Pestis 33 83 53 Np 404241 1 S6, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y pestis 33 83 53 np 404241 1 s6/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    y pestis 33 83 53 np 404241 1 s6 - by Bioz Stars, 2024-03
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