96 well polystyrene microtiter plates  (Thermo Fisher)


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    Thermo Fisher 96 well polystyrene microtiter plates
    96 Well Polystyrene Microtiter Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well polystyrene microtiter plates/product/Thermo Fisher
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    96 well polystyrene microtiter plates - by Bioz Stars, 2020-02
    92/100 stars

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    Blocking Assay:

    Article Title: Epitope Mapping of SERCA2a Identifies an Antigenic Determinant that Induces Mainly Atrial Myocarditis in A/J mice
    Article Snippet: In brief, 96-well polystyrene microtiter plates were coated with or without SERCA2a 971-990 or irrelevant control (RNase 43-56) (10 μg/ml) in 1x coating buffer (eBioscience) and the plates were incubated at 4°C overnight. .. After washing with 1x PBS/0.05% Tween-20 and blocking with 1x PBS/2% BSA/5% normal goat serum for 1.5 hours at RT, serum samples (1:100) were added in duplicates, and the plates were incubated at 37°C for 1 hour.

    Mouse Assay:

    Article Title: Epitope Mapping of SERCA2a Identifies an Antigenic Determinant that Induces Mainly Atrial Myocarditis in A/J mice
    Article Snippet: Serum samples were collected from mice immunized with or without SERCA2a 971-990 on day 21 post-immunization for measurement of SERCA2a-reactive Abs by ELISA. .. In brief, 96-well polystyrene microtiter plates were coated with or without SERCA2a 971-990 or irrelevant control (RNase 43-56) (10 μg/ml) in 1x coating buffer (eBioscience) and the plates were incubated at 4°C overnight.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Epitope Mapping of SERCA2a Identifies an Antigenic Determinant that Induces Mainly Atrial Myocarditis in A/J mice
    Article Snippet: Serum samples were collected from mice immunized with or without SERCA2a 971-990 on day 21 post-immunization for measurement of SERCA2a-reactive Abs by ELISA. .. In brief, 96-well polystyrene microtiter plates were coated with or without SERCA2a 971-990 or irrelevant control (RNase 43-56) (10 μg/ml) in 1x coating buffer (eBioscience) and the plates were incubated at 4°C overnight.

    Concentration Assay:

    Article Title: A Multilaboratory, Multicountry Study To Determine MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing of Selected First-Line Antituberculosis Drugs, Second-Line Injectables, Fluoroquinolones, Clofazimine, and Linezolid
    Article Snippet: .. The frozen 96-well polystyrene microtiter plates contained prediluted drugs at 2× the final concentration made in 2× 7H9-S (Thermo Fisher Sensititre for research use only). .. The concentration ranges in the MIC frozen panels for the drugs tested are summarized in .

    Incubation:

    Article Title: Epitope Mapping of SERCA2a Identifies an Antigenic Determinant that Induces Mainly Atrial Myocarditis in A/J mice
    Article Snippet: .. In brief, 96-well polystyrene microtiter plates were coated with or without SERCA2a 971-990 or irrelevant control (RNase 43-56) (10 μg/ml) in 1x coating buffer (eBioscience) and the plates were incubated at 4°C overnight. .. After washing with 1x PBS/0.05% Tween-20 and blocking with 1x PBS/2% BSA/5% normal goat serum for 1.5 hours at RT, serum samples (1:100) were added in duplicates, and the plates were incubated at 37°C for 1 hour.

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    Thermo Fisher 96 well polystyrene microtiter plates
    Characterization of the DGC PA3177. (A) E. coli BL21-pET3177 and the vector control strain BL21-pET23a were grown in LB broth supplemented with 0.4 mM IPTG for 5 h at 37°C followed by nucleotide extraction and quantification of intracellular c-di-GMP by LC-MS. Levels of c-di-GMP were normalized to the total protein content of the respective sample and compared to c-di-GMP levels in empty vector control strain BL21-pET23a ( n = 6). The chromatogram shows c-di-GMP peaks of one representative measurement. (B) Attachment of P. aeruginosa during PA3177 overexpression was evaluated by crystal violet staining. PAO1-pJN3177 and empty vector control strain PAO1-pJN105 were incubated in <t>96-well</t> <t>microtiter</t> plates for 2 h at 37°C followed by biomass quantification. For recombinant gene expression, BM2 was supplemented with 0.1% (w/v) arabinose (+Ara). Cultures without arabinose (–Ara) served as negative controls. Experiments were carried out in triplicate, each with six wells per strain and condition ( n = 18). (C) Planktonic growth during 5 h at 37°C. (D) Overnight cultures of PA3177-overexpressing strain P. aeruginosa PA01-pJN3177 and vector control PAO1-pJN105 were diluted in LB broth supplemented with 0.1% (w/v) arabinose and grown for 5 h at 37°C followed by evaluation of swimming, swarming and twitching motility. Assays were carried out with three independent bacterial cultures and at least four agar plates per experiment ( n ≥ 12). In all experiments statistical significance was evaluated by the Mann-Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.
    96 Well Polystyrene Microtiter Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well polystyrene microtiter plates/product/Thermo Fisher
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    96 well polystyrene microtiter plates - by Bioz Stars, 2020-02
    90/100 stars
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    79
    Thermo Fisher black welled polystyrene microtitre plate
    Expression of the viscA gene per biomass unit in biofilms (blue diamonds) and planktonic cells (red squares) in the initial state of biofilms grown in <t>microtitre</t> wells. Data represent mean ± sd for a representative experiment with five replicates. *Significant difference between biofilm and planktonic cells at each time point ( P
    Black Welled Polystyrene Microtitre Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black welled polystyrene microtitre plate/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    black welled polystyrene microtitre plate - by Bioz Stars, 2020-02
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    Characterization of the DGC PA3177. (A) E. coli BL21-pET3177 and the vector control strain BL21-pET23a were grown in LB broth supplemented with 0.4 mM IPTG for 5 h at 37°C followed by nucleotide extraction and quantification of intracellular c-di-GMP by LC-MS. Levels of c-di-GMP were normalized to the total protein content of the respective sample and compared to c-di-GMP levels in empty vector control strain BL21-pET23a ( n = 6). The chromatogram shows c-di-GMP peaks of one representative measurement. (B) Attachment of P. aeruginosa during PA3177 overexpression was evaluated by crystal violet staining. PAO1-pJN3177 and empty vector control strain PAO1-pJN105 were incubated in 96-well microtiter plates for 2 h at 37°C followed by biomass quantification. For recombinant gene expression, BM2 was supplemented with 0.1% (w/v) arabinose (+Ara). Cultures without arabinose (–Ara) served as negative controls. Experiments were carried out in triplicate, each with six wells per strain and condition ( n = 18). (C) Planktonic growth during 5 h at 37°C. (D) Overnight cultures of PA3177-overexpressing strain P. aeruginosa PA01-pJN3177 and vector control PAO1-pJN105 were diluted in LB broth supplemented with 0.1% (w/v) arabinose and grown for 5 h at 37°C followed by evaluation of swimming, swarming and twitching motility. Assays were carried out with three independent bacterial cultures and at least four agar plates per experiment ( n ≥ 12). In all experiments statistical significance was evaluated by the Mann-Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Journal: Frontiers in Microbiology

    Article Title: The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

    doi: 10.3389/fmicb.2017.02311

    Figure Lengend Snippet: Characterization of the DGC PA3177. (A) E. coli BL21-pET3177 and the vector control strain BL21-pET23a were grown in LB broth supplemented with 0.4 mM IPTG for 5 h at 37°C followed by nucleotide extraction and quantification of intracellular c-di-GMP by LC-MS. Levels of c-di-GMP were normalized to the total protein content of the respective sample and compared to c-di-GMP levels in empty vector control strain BL21-pET23a ( n = 6). The chromatogram shows c-di-GMP peaks of one representative measurement. (B) Attachment of P. aeruginosa during PA3177 overexpression was evaluated by crystal violet staining. PAO1-pJN3177 and empty vector control strain PAO1-pJN105 were incubated in 96-well microtiter plates for 2 h at 37°C followed by biomass quantification. For recombinant gene expression, BM2 was supplemented with 0.1% (w/v) arabinose (+Ara). Cultures without arabinose (–Ara) served as negative controls. Experiments were carried out in triplicate, each with six wells per strain and condition ( n = 18). (C) Planktonic growth during 5 h at 37°C. (D) Overnight cultures of PA3177-overexpressing strain P. aeruginosa PA01-pJN3177 and vector control PAO1-pJN105 were diluted in LB broth supplemented with 0.1% (w/v) arabinose and grown for 5 h at 37°C followed by evaluation of swimming, swarming and twitching motility. Assays were carried out with three independent bacterial cultures and at least four agar plates per experiment ( n ≥ 12). In all experiments statistical significance was evaluated by the Mann-Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Article Snippet: Attachment Assay in Microtiter Plates Attachment assays in 96-well polystyrene microtiter plates (Nunc, Thermo Fisher Scientific, St. Leon-Rot, Germany) were performed as previously described ( ) with the following minor modifications.

    Techniques: Plasmid Preparation, Liquid Chromatography with Mass Spectroscopy, Over Expression, Staining, Incubation, Recombinant, Expressing, Acetylene Reduction Assay, MANN-WHITNEY

    Implication of c-di-GMP in the response of P. aeruginosa PAO1 to NaClO. (A) P. aeruginosa PAO1 was incubated with NaClO (8 μg/ml, OD 600 = 1.0, BM2) for 1h followed by nucleotide extraction and quantification of intracellular c-di-GMP by LC-MS. Levels of c-di-GMP were normalized to the total protein content of the respective sample and compared to c-di-GMP levels in untreated controls ( n = 6). The chromatogram shows c-di-GMP peaks of one representative measurement. (B) Attachment of P. aeruginosa in response to NaClO during overexpression of the c-di-GMP-degrading PDE PA2133 was evaluated by crystal violet staining. PAO1-pJN2133 and the vector control strain PAO1-pJN105 were incubated in 96-well microtiter plates either in the presence or in the absence of NaClO (2 μg/ml) for 2 h at 37°C followed by the quantification of biofilm biomass. BM2 was supplemented with 0.1% (w/v) arabinose to induce recombinant gene expression. Experiments were repeated four times, each with six wells per strain and condition ( n = 24). Absorbance at 595nm (A 595 ) was determined and obtained values for NaClO-treated samples were normalized against the A 595 of respective untreated controls (=relative biomass). Statistical significance was evaluated by the Mann–Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Journal: Frontiers in Microbiology

    Article Title: The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

    doi: 10.3389/fmicb.2017.02311

    Figure Lengend Snippet: Implication of c-di-GMP in the response of P. aeruginosa PAO1 to NaClO. (A) P. aeruginosa PAO1 was incubated with NaClO (8 μg/ml, OD 600 = 1.0, BM2) for 1h followed by nucleotide extraction and quantification of intracellular c-di-GMP by LC-MS. Levels of c-di-GMP were normalized to the total protein content of the respective sample and compared to c-di-GMP levels in untreated controls ( n = 6). The chromatogram shows c-di-GMP peaks of one representative measurement. (B) Attachment of P. aeruginosa in response to NaClO during overexpression of the c-di-GMP-degrading PDE PA2133 was evaluated by crystal violet staining. PAO1-pJN2133 and the vector control strain PAO1-pJN105 were incubated in 96-well microtiter plates either in the presence or in the absence of NaClO (2 μg/ml) for 2 h at 37°C followed by the quantification of biofilm biomass. BM2 was supplemented with 0.1% (w/v) arabinose to induce recombinant gene expression. Experiments were repeated four times, each with six wells per strain and condition ( n = 24). Absorbance at 595nm (A 595 ) was determined and obtained values for NaClO-treated samples were normalized against the A 595 of respective untreated controls (=relative biomass). Statistical significance was evaluated by the Mann–Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Article Snippet: Attachment Assay in Microtiter Plates Attachment assays in 96-well polystyrene microtiter plates (Nunc, Thermo Fisher Scientific, St. Leon-Rot, Germany) were performed as previously described ( ) with the following minor modifications.

    Techniques: Incubation, Liquid Chromatography with Mass Spectroscopy, Over Expression, Staining, Plasmid Preparation, Recombinant, Expressing, MANN-WHITNEY

    Attachment and DGC expression in PAO1-PA3177Ω in response to NaClO. (A) Attachment of P. aeruginosa mutant strain PAO1-PA3177Ω and wildtype PAO1 after 2 h incubation with half-MIC concentrations of NaClO (2 μg/ml) compared to untreated controls was assayed in 96-well microtiter plates by crystal violet staining. To show alterations in attachment caused by NaClO, A 595 of treated samples was normalized to A 595 of untreated controls for each strain (=relative biomass). Statistical significance was evaluated by the Mann–Whitney test ( ∗∗∗ p ≤ 0.001, n ≥ 12). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers. (B) P. aeruginosa mutant strain PAO1-PA3177Ω was treated with 2 μg/ml NaClO and relative gene expression levels of genes encoding DGCs in comparison to untreated control cultures were analyzed by qRT-PCR. The figure shows mean averages and standard deviations calculated from three experiments, each analyzed in duplicate ( n = 6). Ct values were normalized against the expression of housekeeping genes rpoD and fabD . The red line corresponds to a relative gene expression level of 1, which means no change in gene expression. Blue bars indicate relative gene expression values ≥ 3.

    Journal: Frontiers in Microbiology

    Article Title: The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

    doi: 10.3389/fmicb.2017.02311

    Figure Lengend Snippet: Attachment and DGC expression in PAO1-PA3177Ω in response to NaClO. (A) Attachment of P. aeruginosa mutant strain PAO1-PA3177Ω and wildtype PAO1 after 2 h incubation with half-MIC concentrations of NaClO (2 μg/ml) compared to untreated controls was assayed in 96-well microtiter plates by crystal violet staining. To show alterations in attachment caused by NaClO, A 595 of treated samples was normalized to A 595 of untreated controls for each strain (=relative biomass). Statistical significance was evaluated by the Mann–Whitney test ( ∗∗∗ p ≤ 0.001, n ≥ 12). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers. (B) P. aeruginosa mutant strain PAO1-PA3177Ω was treated with 2 μg/ml NaClO and relative gene expression levels of genes encoding DGCs in comparison to untreated control cultures were analyzed by qRT-PCR. The figure shows mean averages and standard deviations calculated from three experiments, each analyzed in duplicate ( n = 6). Ct values were normalized against the expression of housekeeping genes rpoD and fabD . The red line corresponds to a relative gene expression level of 1, which means no change in gene expression. Blue bars indicate relative gene expression values ≥ 3.

    Article Snippet: Attachment Assay in Microtiter Plates Attachment assays in 96-well polystyrene microtiter plates (Nunc, Thermo Fisher Scientific, St. Leon-Rot, Germany) were performed as previously described ( ) with the following minor modifications.

    Techniques: Expressing, Mutagenesis, Incubation, Staining, MANN-WHITNEY, Quantitative RT-PCR

    Attachment assays with PAO1 mutants. (A) Attachment of P. aeruginosa PAO1 wildtype (WT) or PAO1 mutant strains during overexpression of PA3177 was evaluated by crystal violet staining. Bacteria were incubated in 96-well microtiter plates for 2 h at 37°C prior to biomass quantification. For recombinant gene expression, L was supplemented with 0.1% (w/v) arabinose (+). Cultures without arabinose (-) served as negative controls. (B) Attachment of P. aeruginosa PAO1 WT or PAO1 deletion mutants during NaClO treatment (2 μg/ml in BM2, 2 h) was evaluated by crystal violet staining. All experiments were carried out in triplicate, each with six wells per strain and condition ( n = 18). Statistical significance was evaluated by the Mann–Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Journal: Frontiers in Microbiology

    Article Title: The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

    doi: 10.3389/fmicb.2017.02311

    Figure Lengend Snippet: Attachment assays with PAO1 mutants. (A) Attachment of P. aeruginosa PAO1 wildtype (WT) or PAO1 mutant strains during overexpression of PA3177 was evaluated by crystal violet staining. Bacteria were incubated in 96-well microtiter plates for 2 h at 37°C prior to biomass quantification. For recombinant gene expression, L was supplemented with 0.1% (w/v) arabinose (+). Cultures without arabinose (-) served as negative controls. (B) Attachment of P. aeruginosa PAO1 WT or PAO1 deletion mutants during NaClO treatment (2 μg/ml in BM2, 2 h) was evaluated by crystal violet staining. All experiments were carried out in triplicate, each with six wells per strain and condition ( n = 18). Statistical significance was evaluated by the Mann–Whitney test ( ∗∗∗ p ≤ 0.001, ∗∗ p ≤ 0.01, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Article Snippet: Attachment Assay in Microtiter Plates Attachment assays in 96-well polystyrene microtiter plates (Nunc, Thermo Fisher Scientific, St. Leon-Rot, Germany) were performed as previously described ( ) with the following minor modifications.

    Techniques: Mutagenesis, Over Expression, Staining, Incubation, Recombinant, Expressing, MANN-WHITNEY

    Attachment of P. aeruginosa PAO1 in the presence of different oxidants. Attachment of P. aeruginosa PAO1 during 2 h incubation in the presence of sublethal NaClO concentrations (A–C) or half-MIC concentrations of oxidants NaClO (2 μg/ml), Ca(ClO) 2 (2 μg/ml), NH 2 Cl (equivalent to 2 μg/ml NaClO), H 2 O 2 (50 μg/ml) and paraquat (1 μg/ml) (D) . (A,B,D) Attachment was assayed in 96-well microtiter plates by crystal violet staining and subsequent measurement of A 595 . Obtained values for treated samples were normalized against A 595 of untreated controls (=relative biomass). Experiments were performed at least in triplicate, each with multiple wells per condition ( n ≥ 18). (C) P. aeruginosa PAO1 was incubated with sub-MIC concentrations of NaClO (2 μg/ml) in BM2 for 2 h in 50 ml reaction tubes containing glass microscope slides. Adhered bacteria were stained with the DNA-intercalating dye SYTO9 prior to visualization by fluorescence microscopy at 100× magnification (scale bar: 100 μm). Average surface coverages from 36 pictures per condition were calculated using ImageJ . Experiments were performed in triplicate. For all experiments, statistical significance was evaluated by the Mann-Whitney test ( ∗∗∗ p ≤ 0.001, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Journal: Frontiers in Microbiology

    Article Title: The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa

    doi: 10.3389/fmicb.2017.02311

    Figure Lengend Snippet: Attachment of P. aeruginosa PAO1 in the presence of different oxidants. Attachment of P. aeruginosa PAO1 during 2 h incubation in the presence of sublethal NaClO concentrations (A–C) or half-MIC concentrations of oxidants NaClO (2 μg/ml), Ca(ClO) 2 (2 μg/ml), NH 2 Cl (equivalent to 2 μg/ml NaClO), H 2 O 2 (50 μg/ml) and paraquat (1 μg/ml) (D) . (A,B,D) Attachment was assayed in 96-well microtiter plates by crystal violet staining and subsequent measurement of A 595 . Obtained values for treated samples were normalized against A 595 of untreated controls (=relative biomass). Experiments were performed at least in triplicate, each with multiple wells per condition ( n ≥ 18). (C) P. aeruginosa PAO1 was incubated with sub-MIC concentrations of NaClO (2 μg/ml) in BM2 for 2 h in 50 ml reaction tubes containing glass microscope slides. Adhered bacteria were stained with the DNA-intercalating dye SYTO9 prior to visualization by fluorescence microscopy at 100× magnification (scale bar: 100 μm). Average surface coverages from 36 pictures per condition were calculated using ImageJ . Experiments were performed in triplicate. For all experiments, statistical significance was evaluated by the Mann-Whitney test ( ∗∗∗ p ≤ 0.001, n.s., not significant). Boxes include median (thick horizontal line), 25th and 75th percentiles. Dots indicate extreme values considered as outliers.

    Article Snippet: Attachment Assay in Microtiter Plates Attachment assays in 96-well polystyrene microtiter plates (Nunc, Thermo Fisher Scientific, St. Leon-Rot, Germany) were performed as previously described ( ) with the following minor modifications.

    Techniques: Incubation, Staining, Microscopy, Fluorescence, MANN-WHITNEY

    Adherence of S. pneumoniae to human epithelial cells ( A ) and in vitro growth ( B ). Detroit nasopharyngeal epithelial cell lines were exposed to non-Ec- Sp containing or lacking ICE 1 Sp ST344 and RD 1 Sp ST344, respectively ( A ). Adherence was determined at 30 min and calculated as the proportion of recovered bacteria to the inoculum. Experiments were repeated three times (three times on three different days: Indicated are mean and SD (standard deviation)). Swiss and Thai strains were classic and sporadic non-Ec- Sp , respectively. For the nine adherence experiments, ordinary one-way ANOVA resulted in P = 0.0052. See text for details. MLST, multilocus sequence type. Isolates of the BC3-NT lineage have been recently defined ( Chewapreecha et al. 2014 ). Measurement of planktonic growth was done in 96-well microtiter polystyrene plates and OD450nm was measured on an ELISA plate reader every 30 min for 22 h ( B ). Each experiment included three technical replicates and each experiment was performed three times.

    Journal: Genome Biology and Evolution

    Article Title: Global Phylogenomic Analysis of Nonencapsulated Streptococcus pneumoniae Reveals a Deep-Branching Classic Lineage That Is Distinct from Multiple Sporadic Lineages

    doi: 10.1093/gbe/evu263

    Figure Lengend Snippet: Adherence of S. pneumoniae to human epithelial cells ( A ) and in vitro growth ( B ). Detroit nasopharyngeal epithelial cell lines were exposed to non-Ec- Sp containing or lacking ICE 1 Sp ST344 and RD 1 Sp ST344, respectively ( A ). Adherence was determined at 30 min and calculated as the proportion of recovered bacteria to the inoculum. Experiments were repeated three times (three times on three different days: Indicated are mean and SD (standard deviation)). Swiss and Thai strains were classic and sporadic non-Ec- Sp , respectively. For the nine adherence experiments, ordinary one-way ANOVA resulted in P = 0.0052. See text for details. MLST, multilocus sequence type. Isolates of the BC3-NT lineage have been recently defined ( Chewapreecha et al. 2014 ). Measurement of planktonic growth was done in 96-well microtiter polystyrene plates and OD450nm was measured on an ELISA plate reader every 30 min for 22 h ( B ). Each experiment included three technical replicates and each experiment was performed three times.

    Article Snippet: Adherence to Human Epithelial Cell Line Detroit 562 and Analysis of Planktonic Growth As for measuring planktonic growth, 96-well microtiter polystyrene plates (Thermo Fisher Scientific, Denmark) were used for the isolates Switzerland 4, 11, Thailand 1 and 2, respectively.

    Techniques: In Vitro, Standard Deviation, Sequencing, Enzyme-linked Immunosorbent Assay

    Biofilm formation and rapid attachment. Bacteria were grown for 24 h (A) or for 1 h (B) at 37°C in 96-well microtiter plates containing BM2 biofilm medium. Biofilm formation and adhesion ability were analyzed by staining of the adherent biomass with crystal violet, followed by quantification ( A 595 ) for the stained wells. Shown are the results of at least three independent biological experiments, each with six technical repeats. The statistical significance of differences between the PA4398 − mutant and WT PA14, as well as between the PA4398c and pUCP20 strains, was determined by the Mann-Whitney test (***, P ≤ 0.001; n. s., not significant). The highest and lowest outliers are indicated by “×.”

    Journal: Applied and Environmental Microbiology

    Article Title: Sensor Kinase PA4398 Modulates Swarming Motility and Biofilm Formation in Pseudomonas aeruginosa PA14

    doi: 10.1128/AEM.02832-14

    Figure Lengend Snippet: Biofilm formation and rapid attachment. Bacteria were grown for 24 h (A) or for 1 h (B) at 37°C in 96-well microtiter plates containing BM2 biofilm medium. Biofilm formation and adhesion ability were analyzed by staining of the adherent biomass with crystal violet, followed by quantification ( A 595 ) for the stained wells. Shown are the results of at least three independent biological experiments, each with six technical repeats. The statistical significance of differences between the PA4398 − mutant and WT PA14, as well as between the PA4398c and pUCP20 strains, was determined by the Mann-Whitney test (***, P ≤ 0.001; n. s., not significant). The highest and lowest outliers are indicated by “×.”

    Article Snippet: Biofilm formation was analyzed in 96-well polystyrene microtiter plates (Nunc, Thermo Fisher Scientific, St. Leon-Rot, Germany) by using an abiotic solid-surface assay ( ).

    Techniques: Staining, Mutagenesis, MANN-WHITNEY

    Expression of the viscA gene per biomass unit in biofilms (blue diamonds) and planktonic cells (red squares) in the initial state of biofilms grown in microtitre wells. Data represent mean ± sd for a representative experiment with five replicates. *Significant difference between biofilm and planktonic cells at each time point ( P

    Journal: Microbiology

    Article Title: Lipopeptide biosurfactant viscosin enhances dispersal of Pseudomonas fluorescens SBW25 biofilms

    doi: 10.1099/mic.0.000191

    Figure Lengend Snippet: Expression of the viscA gene per biomass unit in biofilms (blue diamonds) and planktonic cells (red squares) in the initial state of biofilms grown in microtitre wells. Data represent mean ± sd for a representative experiment with five replicates. *Significant difference between biofilm and planktonic cells at each time point ( P

    Article Snippet: The culture was aliquoted (125 μl) into wells of a black-welled polystyrene microtitre plate (MicroWell 96-well optical-bottom, non-treated; Thermo Scientific Nunc) and biofilm was allowed to develop at room temperature as described previously.

    Techniques: Expressing

    Biofilm formation of P. fluorescens SBW25 (dark bars) and P. fluorescens SBW25Δ viscA (light bars) in AB minimal medium with citrate. The biofilm was quantified from 7.5 to 17.5 h using the crystal violet assay. The A 590 value represents crystal violet-stained biofilm attached to the walls of the microtitre wells and is an indirect measure of the biofilm formed. Data represent mean ± sd for a representative experiment with eight replicates. *Significant difference between strains at each time point ( P

    Journal: Microbiology

    Article Title: Lipopeptide biosurfactant viscosin enhances dispersal of Pseudomonas fluorescens SBW25 biofilms

    doi: 10.1099/mic.0.000191

    Figure Lengend Snippet: Biofilm formation of P. fluorescens SBW25 (dark bars) and P. fluorescens SBW25Δ viscA (light bars) in AB minimal medium with citrate. The biofilm was quantified from 7.5 to 17.5 h using the crystal violet assay. The A 590 value represents crystal violet-stained biofilm attached to the walls of the microtitre wells and is an indirect measure of the biofilm formed. Data represent mean ± sd for a representative experiment with eight replicates. *Significant difference between strains at each time point ( P

    Article Snippet: The culture was aliquoted (125 μl) into wells of a black-welled polystyrene microtitre plate (MicroWell 96-well optical-bottom, non-treated; Thermo Scientific Nunc) and biofilm was allowed to develop at room temperature as described previously.

    Techniques: Crystal Violet Assay, Staining