96 well pcr plates  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Thermo Fisher 96 well pcr plates
    Workflow outlining the major steps of the study. SYBR Green I-stained single sperm cells were isolated by fluorescence-activated cell sorting (FACS) to <t>96-well</t> <t>PCR</t> plates; DNA was amplified and used for next-generation sequencing
    96 Well Pcr Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well pcr plates/product/Thermo Fisher
    Average 95 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    96 well pcr plates - by Bioz Stars, 2020-05
    95/100 stars

    Images

    1) Product Images from "Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing"

    Article Title: Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing

    Journal: Journal of Assisted Reproduction and Genetics

    doi: 10.1007/s10815-018-1340-0

    Workflow outlining the major steps of the study. SYBR Green I-stained single sperm cells were isolated by fluorescence-activated cell sorting (FACS) to 96-well PCR plates; DNA was amplified and used for next-generation sequencing
    Figure Legend Snippet: Workflow outlining the major steps of the study. SYBR Green I-stained single sperm cells were isolated by fluorescence-activated cell sorting (FACS) to 96-well PCR plates; DNA was amplified and used for next-generation sequencing

    Techniques Used: SYBR Green Assay, Staining, Isolation, Fluorescence, FACS, Polymerase Chain Reaction, Amplification, Next-Generation Sequencing

    Related Articles

    SYBR Green Assay:

    Article Title: Interferon-β Stimulation Elicited by the Influenza Virus Is Regulated by the Histone Methylase Dot1L through the RIG-I-TRIM25 Signaling Axis
    Article Snippet: .. PCR was performed in 96-well PCR plates using the SYBR green PCR master mix (Applied Biosystems). .. PCR was carried out in a PRISM 7500 Sequence detection system (Applied Biosystems).

    Quantitative RT-PCR:

    Article Title: Gene Expression Dynamics at the Neurovascular Unit During Early Regeneration After Cerebral Ischemia/Reperfusion Injury in Mice
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) was performed using TaqMan Gene Expression Assays in Applied Biosystems 96-well PCR plates (Thermo Fisher Scientific). .. Plates were sealed with Clear Seal Diamond Heat Sealing Film (Thermo Fisher Scientific) and run in an AB7500 fast system (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, United States).

    Real-time Polymerase Chain Reaction:

    Article Title: Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulator
    Article Snippet: .. Thermal self-quenching assays were conducted in plate format (96- or 384-well, 10 μl solution volume) using the Mx3500P qPCR system (Agilent Technologies) for 96-well PCR plates and the ViiA 7 real-time PCR system (Life Technologies, Inc.) for 384-well PCR plates. .. Both systems used a heating rate of 3 °C/min and fluorescence excitation and emission wavelengths of 545 and 568 nm, respectively.

    Article Title: Gene Expression Dynamics at the Neurovascular Unit During Early Regeneration After Cerebral Ischemia/Reperfusion Injury in Mice
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) was performed using TaqMan Gene Expression Assays in Applied Biosystems 96-well PCR plates (Thermo Fisher Scientific). .. Plates were sealed with Clear Seal Diamond Heat Sealing Film (Thermo Fisher Scientific) and run in an AB7500 fast system (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, United States).

    Article Title: Identification of microRNAs involved in NOD-dependent induction of pro-inflammatory genes in pulmonary endothelial cells.
    Article Snippet: .. Real-time PCR was performed in duplicate in a total volume of 20 μL using Power SYBR green PCR master mixture (Thermo Fisher Scientific) or real-time PCR probes and TaqMan Fast Advanced MasterMix (Thermo Fisher Scientific) on a Step One Plus real-time PCR system (Applied Biosystems, Foster City, CA) in 96-well PCR plates (Applied Biosystems). .. SYBR Green or FAM/ BHQ-1 fluorescence emissions were monitored after each cycle.

    Article Title: Identification of microRNAs involved in NOD-dependent induction of pro-inflammatory genes in pulmonary endothelial cells.
    Article Snippet: .. Real-time PCR was performed in a total volume of 20 μL on a Step One Plus real-time PCR system (Applied Biosystems) in 96-well PCR plates (Applied Biosystems). .. FAM/MGB-NFQ fluorescence emissions were monitored after each cycle.

    Expressing:

    Article Title: Gene Expression Dynamics at the Neurovascular Unit During Early Regeneration After Cerebral Ischemia/Reperfusion Injury in Mice
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) was performed using TaqMan Gene Expression Assays in Applied Biosystems 96-well PCR plates (Thermo Fisher Scientific). .. Plates were sealed with Clear Seal Diamond Heat Sealing Film (Thermo Fisher Scientific) and run in an AB7500 fast system (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, United States).

    Polymerase Chain Reaction:

    Article Title: Interferon-β Stimulation Elicited by the Influenza Virus Is Regulated by the Histone Methylase Dot1L through the RIG-I-TRIM25 Signaling Axis
    Article Snippet: .. PCR was performed in 96-well PCR plates using the SYBR green PCR master mix (Applied Biosystems). .. PCR was carried out in a PRISM 7500 Sequence detection system (Applied Biosystems).

    Article Title: Total mRNA Quantification in Single Cells: Sarcoma Cell Heterogeneity
    Article Snippet: .. Single cells were sorted into 96-well PCR plates (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) with 5 µL lysis buffer containing 1 µg/µL bovine serum albumin supplied in 2.5% glycerol and 0.2% Triton X-100. ..

    Article Title: Ligand binding to a remote site thermodynamically corrects the F508del mutation in the human cystic fibrosis transmembrane conductance regulator
    Article Snippet: .. Thermal self-quenching assays were conducted in plate format (96- or 384-well, 10 μl solution volume) using the Mx3500P qPCR system (Agilent Technologies) for 96-well PCR plates and the ViiA 7 real-time PCR system (Life Technologies, Inc.) for 384-well PCR plates. .. Both systems used a heating rate of 3 °C/min and fluorescence excitation and emission wavelengths of 545 and 568 nm, respectively.

    Article Title: Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing
    Article Snippet: .. Single sperms were collected into 96-well PCR plates (Thermo Fisher Scientific, USA) containing 3 μL PBS per well. ..

    Article Title: Gene Expression Dynamics at the Neurovascular Unit During Early Regeneration After Cerebral Ischemia/Reperfusion Injury in Mice
    Article Snippet: .. Quantitative Real Time PCR (qRT-PCR) was performed using TaqMan Gene Expression Assays in Applied Biosystems 96-well PCR plates (Thermo Fisher Scientific). .. Plates were sealed with Clear Seal Diamond Heat Sealing Film (Thermo Fisher Scientific) and run in an AB7500 fast system (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, United States).

    Article Title: Acute effects of viral respiratory tract infections on sputum bacterial density during CF pulmonary exacerbations
    Article Snippet: .. PCR was performed in 10 μl volumes in 96-well PCR plates using TaqMan Fast Advanced master mix (Life Technologies). .. Thermocycling was performed using a ViiA7 thermocycler (Life Technologies) in a fast mode, with an initial 20 seconds (s) at 95 °C followed by 40 cycles of two-temperature cycling at 95 °C for 3 s, followed by 60 °C for 30 s. Cycle threshold (Ct) values were obtained using the ViiA7 software, and then exported to Excel for analysis.

    Article Title: Identification of microRNAs involved in NOD-dependent induction of pro-inflammatory genes in pulmonary endothelial cells.
    Article Snippet: .. Real-time PCR was performed in a total volume of 20 μL on a Step One Plus real-time PCR system (Applied Biosystems) in 96-well PCR plates (Applied Biosystems). .. FAM/MGB-NFQ fluorescence emissions were monitored after each cycle.

    Lysis:

    Article Title: Total mRNA Quantification in Single Cells: Sarcoma Cell Heterogeneity
    Article Snippet: .. Single cells were sorted into 96-well PCR plates (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) with 5 µL lysis buffer containing 1 µg/µL bovine serum albumin supplied in 2.5% glycerol and 0.2% Triton X-100. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher taqman array 96 well human wnt pathway plates
    Inhibition of miR-194 results in downregulation of <t>Wnt/β-catenin</t> signalling-associated genes. (A) Transfection efficiency of β-catenin siRNA was evaluated prior to the <t>TaqMan</t> ® Wnt plate assay. BGC-823 cells were transfected with NC siRNA or β-catenin siRNA, and 48 h post-transfection, the cell lysates were immunoblotted with a β-catenin antibody. (B) RT-qPCR of β-catenin was performed on BGC-823 cells 48 h post-transfection with β-catenin siRNA. Student's t-test was performed for statistical analysis. *P
    Taqman Array 96 Well Human Wnt Pathway Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman array 96 well human wnt pathway plates/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    taqman array 96 well human wnt pathway plates - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    95
    Thermo Fisher 96 well pcr plates
    Workflow outlining the major steps of the study. SYBR Green I-stained single sperm cells were isolated by fluorescence-activated cell sorting (FACS) to <t>96-well</t> <t>PCR</t> plates; DNA was amplified and used for next-generation sequencing
    96 Well Pcr Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well pcr plates/product/Thermo Fisher
    Average 95 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    96 well pcr plates - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    93
    Thermo Fisher control sirna
    Silencing of <t>CDKN2B</t> and cell proliferation. CCL210 fibroblasts were treated with either control <t>siRNA</t> or siRNA targeted against CDKN2B. A) Levels of CDKN2B mRNA was assayed by RT-PCR (n = 3). B) Cell proliferation was measured by the CyQuant assay. Shown are the mean data from 6 replicates of a representative experiment.
    Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control sirna/product/Thermo Fisher
    Average 93 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    control sirna - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of miR-194 results in downregulation of Wnt/β-catenin signalling-associated genes. (A) Transfection efficiency of β-catenin siRNA was evaluated prior to the TaqMan ® Wnt plate assay. BGC-823 cells were transfected with NC siRNA or β-catenin siRNA, and 48 h post-transfection, the cell lysates were immunoblotted with a β-catenin antibody. (B) RT-qPCR of β-catenin was performed on BGC-823 cells 48 h post-transfection with β-catenin siRNA. Student's t-test was performed for statistical analysis. *P

    Journal: Oncology Reports

    Article Title: Inhibition of miR-194 suppresses the Wnt/β-catenin signalling pathway in gastric cancer

    doi: 10.3892/or.2018.6773

    Figure Lengend Snippet: Inhibition of miR-194 results in downregulation of Wnt/β-catenin signalling-associated genes. (A) Transfection efficiency of β-catenin siRNA was evaluated prior to the TaqMan ® Wnt plate assay. BGC-823 cells were transfected with NC siRNA or β-catenin siRNA, and 48 h post-transfection, the cell lysates were immunoblotted with a β-catenin antibody. (B) RT-qPCR of β-catenin was performed on BGC-823 cells 48 h post-transfection with β-catenin siRNA. Student's t-test was performed for statistical analysis. *P

    Article Snippet: TaqMan® Array 96-well Human WNT Pathway Plates (Thermo Fisher Scientific, Inc.) were used to quantify the expression levels of Wnt signalling-associated genes, according to the manufacturer's protocols.

    Techniques: Inhibition, Transfection, Quantitative RT-PCR

    Workflow outlining the major steps of the study. SYBR Green I-stained single sperm cells were isolated by fluorescence-activated cell sorting (FACS) to 96-well PCR plates; DNA was amplified and used for next-generation sequencing

    Journal: Journal of Assisted Reproduction and Genetics

    Article Title: Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing

    doi: 10.1007/s10815-018-1340-0

    Figure Lengend Snippet: Workflow outlining the major steps of the study. SYBR Green I-stained single sperm cells were isolated by fluorescence-activated cell sorting (FACS) to 96-well PCR plates; DNA was amplified and used for next-generation sequencing

    Article Snippet: Single sperms were collected into 96-well PCR plates (Thermo Fisher Scientific, USA) containing 3 μL PBS per well.

    Techniques: SYBR Green Assay, Staining, Isolation, Fluorescence, FACS, Polymerase Chain Reaction, Amplification, Next-Generation Sequencing

    Silencing of CDKN2B and cell proliferation. CCL210 fibroblasts were treated with either control siRNA or siRNA targeted against CDKN2B. A) Levels of CDKN2B mRNA was assayed by RT-PCR (n = 3). B) Cell proliferation was measured by the CyQuant assay. Shown are the mean data from 6 replicates of a representative experiment.

    Journal: PLoS ONE

    Article Title: Lung Fibroblasts from Patients with Idiopathic Pulmonary Fibrosis Exhibit Genome-Wide Differences in DNA Methylation Compared to Fibroblasts from Nonfibrotic Lung

    doi: 10.1371/journal.pone.0107055

    Figure Lengend Snippet: Silencing of CDKN2B and cell proliferation. CCL210 fibroblasts were treated with either control siRNA or siRNA targeted against CDKN2B. A) Levels of CDKN2B mRNA was assayed by RT-PCR (n = 3). B) Cell proliferation was measured by the CyQuant assay. Shown are the mean data from 6 replicates of a representative experiment.

    Article Snippet: Cell proliferation Cells were plated at 2×103 cells/well in 96-well plates and treated with either control siRNA or siRNA targeting CDKN2B (ON-TARGETplus SMARTpool, Thermo Scientific).

    Techniques: Reverse Transcription Polymerase Chain Reaction, CyQUANT Assay