96 well immulon plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 96 well immulon plates
    96 Well Immulon Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well immulon plates/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    96 well immulon plates - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Positive Control:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Synthesized:

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Following pre-incubation at 37°C for 30 min, a bradykinin-based quenched fluorescent substrate (QFS), (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2, 4-dinitrophenyl) (custom synthesized by Auspep; Johnson and Ahn, ), was added to a final concentration of 40 μM.

    Blocking Assay:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. All plates were blocked with the blocking buffer (3% fatty acid free BSA, 20 mM Tris, 140 mM sodium chloride, 5 mM calcium chloride, pH 7.4) for 1 h at room temperature (RT) under humid conditions.

    Real-time Polymerase Chain Reaction:

    Article Title: Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma
    Article Snippet: .. Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR. ..

    Incubation:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity. .. After 4 h, Gibco Dulbecco's modified Eagle's medium (Thermo Fisher Scientific, Inc.), supplemented with 10% equine serum, was removed from the plates, and the cell cultures were incubated with 2.0% B-27 neurobasal medium for 4 days.

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen). .. PEI and plasmids were mixed together and incubated for 30 min at room temperature.

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: .. Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: Antifungal Activity Determination The MIC of all protein and peptide variants was determined on C. albicans with broth microdilution assays in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. In each well, 103 cells were incubated with increasing concentrations of antifungal proteins or peptides in a final volume of 200 μL 0.05× PDB.

    Activity Assay:

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. The antifungal activity against C. albicans biofilms was determined in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. Cells (105 ) in 100 μL 0.05× PDB were distributed per well and plates were incubated at 37°C for up to 24 h to allow biofilm formation.

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. Antifungal Activity Determination The MIC of all protein and peptide variants was determined on C. albicans with broth microdilution assays in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. From a fresh C. albicans overnight culture on YPD agar, cells were harvested with an inoculation loop and suspended in 0.05× PDB (Sigma-Aldrich) ( ).

    Expressing:

    Article Title: Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma
    Article Snippet: .. Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR. ..

    Modification:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: The sections were then placed into a 0.25% pancreatic enzyme EDTA solution at 37°C for 20 min, followed by termination of digestion by addition of 5 ml Dulbecco's Modified Eagle medium (DMEM; Gibco, Thermo Fisher Scientific, Inc.) supplemented with 10% horse serum and 10% fetal bovine serum for 5 min. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Transfection:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: Cell transfection was performed with a 1 mg/ml poly-ethylenimine (PEI “Max”; Polyscience) solution adjusted at pH = 7. .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Activation Assay:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: Paragraph title: Allogeneic and anti-CD3/CD28–induced CD4+ T-cell activation ... Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway).

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: Paragraph title: T-Cell Activation Assay. ... Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium.

    Transferring:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: The cells were isolated from the sections by a mechanical method using a Pasteur pipette, and passed through a 200 mesh stainless steel sieve. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Cell Culture:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: .. Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway). ..

    Generated:

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Fluorescence generated by cleavage of the QFS was continuously monitored (λ ex = 320 nm, λ em = 405 nm) in a FLUOStar Optima plate reader (BMG LabTech, Offenburg, Germany) maintained at 37°C.

    Imaging:

    Article Title: Plaque2.0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion
    Article Snippet: .. All live imaging experiments were performed in 96-well black plates (Matrix; Thermo Fisher Scientific, Lausanne, Switzerland) and the entire wells were acquired in four sites by a motorized stage, allowing precise, high-speed selection of overlapping tiles. .. Confocal microscopy Confocal fluorescence microscopy was conducted with a Leica SP5 confocal laser scanning microscope (Leica Microsystems, Germany) equipped with a 63x objective (oil immersion; NA 1.4), a UV laser (355 nm), an argon laser (488 nm), and a diode laser (561 nm).

    Protein Concentration:

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: The MIC was defined as the protein concentration that inhibited growth by ≥90% compared to the untreated control, which was referred as 100% growth. .. The antifungal activity against C. albicans biofilms was determined in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific).

    Recombinant:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    MTT Assay:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity. .. Subsequently, the cells were incubated with RDPS (0.025, 0.05, 0.10, 0.25, 0.50, 1.0, 2.0, 4.0 or 8.0 g/l) for 48 h, after which the culture medium (0.1 ml) was removed and added to 96-well plates containing 0.5% MTT for 4 h. Following removal of the culture media, 0.15 ml dimethyl sulfoxide (DMSO; Sigma-Aldrich) was added to the wells, and the plates were agitated for 10 min. Absorbance [optical density (OD)] values were measured at 490 nm using a microplate reader (EL×800™; BioTek Instruments, Inc., Winooski, VT, USA).

    Fluorescence:

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Fluorescence generated by cleavage of the QFS was continuously monitored (λ ex = 320 nm, λ em = 405 nm) in a FLUOStar Optima plate reader (BMG LabTech, Offenburg, Germany) maintained at 37°C.

    Irradiation:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: .. Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. To confirm that NCRD binding was independent of the UV exposure, ELISA experiments were also performed without UV treatment with no significant differences seen in the results (data not shown).

    Multiple Displacement Amplification:

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

    Isolation:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: The cells were isolated from the sections by a mechanical method using a Pasteur pipette, and passed through a 200 mesh stainless steel sieve. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: .. Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. Peptides used were the immunodominant H-2Kb –restricted MHC class I OVA-derived peptide SIINFEKL (InvivoGen) or the H-2 I-Ab MHC class II-restricted peptide ISQAVHAAHAEINEAGR (InvivoGen).

    Detection Assay:

    Article Title: Sulforaphane induces apoptosis in rhabdomyosarcoma and restores TRAIL-sensitivity in the aggressive alveolar subtype leading to tumor elimination in mice
    Article Snippet: Cell viability was determined using the Luminescence ATP Detection Assay System (ATPlite) (PerkinElmer). .. Cells were plated in triplicate using 96-well cluster plates: 20 × 103 CCA cells were plated with 100 μL of OPTI-MEM (GIBCO BRL) containing 4% FCS, 2 mM l -glutamine, and 1% penicillin/streptomycin.

    Microscopy:

    Article Title: Plaque2.0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion
    Article Snippet: Paragraph title: Time-lapse multi-site, multi-channel microscopy ... All live imaging experiments were performed in 96-well black plates (Matrix; Thermo Fisher Scientific, Lausanne, Switzerland) and the entire wells were acquired in four sites by a motorized stage, allowing precise, high-speed selection of overlapping tiles.

    Purification:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: .. Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway). ..

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. Alternatively, 50 μL containing 0.1 μg/μL of purified mycobacterial cell wall components were dried in ethanol onto duplicate or triplicate wells of a 96-well microtiter plate (EIA/RIA, CoStar, Corning, NY).

    Selection:

    Article Title: Plaque2.0—A High-Throughput Analysis Framework to Score Virus-Cell Transmission and Clonal Cell Expansion
    Article Snippet: .. All live imaging experiments were performed in 96-well black plates (Matrix; Thermo Fisher Scientific, Lausanne, Switzerland) and the entire wells were acquired in four sites by a motorized stage, allowing precise, high-speed selection of overlapping tiles. .. Confocal microscopy Confocal fluorescence microscopy was conducted with a Leica SP5 confocal laser scanning microscope (Leica Microsystems, Germany) equipped with a 63x objective (oil immersion; NA 1.4), a UV laser (355 nm), an argon laser (488 nm), and a diode laser (561 nm).

    Mouse Assay:

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: .. Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. Peptides used were the immunodominant H-2Kb –restricted MHC class I OVA-derived peptide SIINFEKL (InvivoGen) or the H-2 I-Ab MHC class II-restricted peptide ISQAVHAAHAEINEAGR (InvivoGen).

    Viability Assay:

    Article Title: Sulforaphane induces apoptosis in rhabdomyosarcoma and restores TRAIL-sensitivity in the aggressive alveolar subtype leading to tumor elimination in mice
    Article Snippet: Paragraph title: Viability assay ... Cells were plated in triplicate using 96-well cluster plates: 20 × 103 CCA cells were plated with 100 μL of OPTI-MEM (GIBCO BRL) containing 4% FCS, 2 mM l -glutamine, and 1% penicillin/streptomycin.

    Plasmid Preparation:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen). .. In parallel, 53 µg of poly(ethyleneimine) from Sigma-Aldrich (PEI) were diluted in 500 µl of DMEM (Gibco-BRL).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Paragraph title: ELISA ... Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria.

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. After 5 d in culture, the concentration of 1:20 diluted supernatant IFN-γ was quantified using IFN-γ ELISA kits (BD Biosciences).

    Negative Control:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Binding Assay:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: Binding of SP-D NCRD mutants to mycobacteria and/or their cell envelope components was performed by ELISA (Schlesinger et al. ). .. Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria.

    Concentration Assay:

    Article Title: Neuroprotective effects of Rhizoma Dioscoreae polysaccharides against neuronal apoptosis induced by in vitro hypoxia
    Article Snippet: Next, the cells were counted and adjusted to a concentration of 5×106 /ml using DMEM. .. Subsequently, 0.1 ml cells were seeded at a density of 5×105 cells/ml into 96-well culture plates coated with polylysine, and were subsequently stored in a 5% CO2 incubator (Thermo Fisher Scientific, Inc.) at 37°C with saturated humidity.

    Article Title: Protein Kinase C Regulates the Cell Surface Activity of Endothelin-Converting Enzyme-1
    Article Snippet: Cell-based Quenched Fluorescent Substrate Assay EA.hy926 cells grown in 96-well microtiter plates were washed with Opti-MEM (Invitrogen Australia, Mt. .. Following pre-incubation at 37°C for 30 min, a bradykinin-based quenched fluorescent substrate (QFS), (7-methoxycoumarin-4-yl)acetyl-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(2, 4-dinitrophenyl) (custom synthesized by Auspep; Johnson and Ahn, ), was added to a final concentration of 40 μM.

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: For each plate, columns 1 and 12 were dedicated to controls: culture wells were alternatively spiked with 1 µl of DMSO alone (negative control) or supplemented with recombinant IFN-β so that final concentration equals 1,000 IU/ml (positive control). .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen).

    Article Title: Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
    Article Snippet: Splenocytes were isolated from mice and plated at a density of 10 million cells per milliliter in 96-well culture plates in the presence of growth medium [all components were from Life Technologies unless otherwise indicated: RPMI 1620 with GlutaMAX supplemented with 8% FBS, 1 mM nonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50 μM 2-mercaptoethanol (Sigma), and penicillin/streptomycin] only or 2 μg/mL OVA-derived peptide in growth medium. .. After 5 d in culture, the concentration of 1:20 diluted supernatant IFN-γ was quantified using IFN-γ ELISA kits (BD Biosciences).

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

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  • 94
    Thermo Fisher immulon 2hb 96 well plates
    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of <t>Immulon</t> 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.
    Immulon 2hb 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon 2hb 96 well plates/product/Thermo Fisher
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    immulon 2hb 96 well plates - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    86
    Thermo Fisher immulon ii hb 96 well elisa plates
    Binding of MS17-57 to lysates of MKN45, BGC823 and GES-1 cell lines in indirect <t>ELISA.</t> MKN45, BGC823, SGC7901, and MKN28 cells were used in experiments, but MKN45 and BGC823 cell lines were randomly selected as examples in this figure. MS17-57 expressed strong binding signals to MKN45 cells and moderate binding signals to BGC823 cells and GES-1 cells. Cell lysates were coated with 1.0 µg/mL PBS onto <t>Immulon-II</t> HB 96-well ELISA plates (100 µL/well). The protein concentrations of these cell lysates were balanced, but not for the binding targets (Ags) that could be a big variation. Irrelevant mAb was used as an isotype control.
    Immulon Ii Hb 96 Well Elisa Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon ii hb 96 well elisa plates/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immulon ii hb 96 well elisa plates - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    91
    Thermo Fisher immulon 2 hb 96 well plates
    Binding of MS17-57 to lysates of MKN45, BGC823 and GES-1 cell lines in indirect <t>ELISA.</t> MKN45, BGC823, SGC7901, and MKN28 cells were used in experiments, but MKN45 and BGC823 cell lines were randomly selected as examples in this figure. MS17-57 expressed strong binding signals to MKN45 cells and moderate binding signals to BGC823 cells and GES-1 cells. Cell lysates were coated with 1.0 µg/mL PBS onto <t>Immulon-II</t> HB 96-well ELISA plates (100 µL/well). The protein concentrations of these cell lysates were balanced, but not for the binding targets (Ags) that could be a big variation. Irrelevant mAb was used as an isotype control.
    Immulon 2 Hb 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immulon 2 hb 96 well plates/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    immulon 2 hb 96 well plates - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of Immulon 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.

    Journal: The Journal of Biological Chemistry

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *

    doi: 10.1074/jbc.M112.353144

    Figure Lengend Snippet: Binding of wild-type α1 I domain to Toolkits II and III and to selected shorter triple-helical peptides. The recombinant wild-type α1 I domain was incubated in wells of Immulon 2HB 96-well plates coated with peptides, and adhesion was measured in the presence of either 2 m m Mg 2+ ( black bars ) or 2 m m EDTA ( white bars ) as described under “Experimental Procedures.” BSA, GPP10, and GFOGER served as control surface coatings. Experiments were performed in triplicate using 0.1 μg of protein/well. a , adhesion to Toolkit II; b , adhesion to Toolkit III; c , adhesion to selected shorter peptides, formatted with the indicated sequence flanked by (GPP) 5 host peptides. Long GFPGER has the same sequence as II-28 but with “O” in its GFOGER motif replaced with “P.” Data are the mean ± S.E. of at least six independent experiments. Col , collagen.

    Article Snippet: Peptides were coated at 10 μg/ml for 1 h at 22 °C on Immulon 2HB 96-well plates (Thermo Scientific) and blocked for 1 h with 200 μl of TBS containing 50 mg/ml BSA.

    Techniques: Binding Assay, Recombinant, Incubation, Sequencing

    Binding of PC12 cells to Toolkits II and III and selected peptides. Immulon 2HB 96-well plates or E-Plates TM for the xCELLigence instrument were coated with the indicated peptides as described under “Experimental Procedures.” a and b , PC12 cells were seeded at 10 5 /well, and SPBA was performed after 1 h as described using the colorimetric lactate dehydrogenase assay kit. c and d , PC12 cells were seeded onto E-Plates at 2.5 × 10 4 cells/well, and the cell index was measured continually. PC12 cell binding to Toolkits II ( a ) and III ( b ) is shown. PC12 cell adhesion to the indicated Toolkit peptides ( c ) and to selected shorter integrin-binding peptides ( d ) is shown as end point data after 2 h of adhesion. All experiments were performed in triplicate in the presence of either 2 m m Mg 2+ or EDTA as indicated. Data are the mean ± S.E. of three independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin ?1?1 Using Collagen Toolkits II and III *

    doi: 10.1074/jbc.M112.353144

    Figure Lengend Snippet: Binding of PC12 cells to Toolkits II and III and selected peptides. Immulon 2HB 96-well plates or E-Plates TM for the xCELLigence instrument were coated with the indicated peptides as described under “Experimental Procedures.” a and b , PC12 cells were seeded at 10 5 /well, and SPBA was performed after 1 h as described using the colorimetric lactate dehydrogenase assay kit. c and d , PC12 cells were seeded onto E-Plates at 2.5 × 10 4 cells/well, and the cell index was measured continually. PC12 cell binding to Toolkits II ( a ) and III ( b ) is shown. PC12 cell adhesion to the indicated Toolkit peptides ( c ) and to selected shorter integrin-binding peptides ( d ) is shown as end point data after 2 h of adhesion. All experiments were performed in triplicate in the presence of either 2 m m Mg 2+ or EDTA as indicated. Data are the mean ± S.E. of three independent experiments.

    Article Snippet: Peptides were coated at 10 μg/ml for 1 h at 22 °C on Immulon 2HB 96-well plates (Thermo Scientific) and blocked for 1 h with 200 μl of TBS containing 50 mg/ml BSA.

    Techniques: Binding Assay, Lactate Dehydrogenase Assay

    Binding of MS17-57 to lysates of MKN45, BGC823 and GES-1 cell lines in indirect ELISA. MKN45, BGC823, SGC7901, and MKN28 cells were used in experiments, but MKN45 and BGC823 cell lines were randomly selected as examples in this figure. MS17-57 expressed strong binding signals to MKN45 cells and moderate binding signals to BGC823 cells and GES-1 cells. Cell lysates were coated with 1.0 µg/mL PBS onto Immulon-II HB 96-well ELISA plates (100 µL/well). The protein concentrations of these cell lysates were balanced, but not for the binding targets (Ags) that could be a big variation. Irrelevant mAb was used as an isotype control.

    Journal: PLoS ONE

    Article Title: Generation of Monoclonal Antibody MS17-57 Targeting Secreted Alkaline Phosphatase Ectopically Expressed on the Surface of Gastrointestinal Cancer Cells

    doi: 10.1371/journal.pone.0077398

    Figure Lengend Snippet: Binding of MS17-57 to lysates of MKN45, BGC823 and GES-1 cell lines in indirect ELISA. MKN45, BGC823, SGC7901, and MKN28 cells were used in experiments, but MKN45 and BGC823 cell lines were randomly selected as examples in this figure. MS17-57 expressed strong binding signals to MKN45 cells and moderate binding signals to BGC823 cells and GES-1 cells. Cell lysates were coated with 1.0 µg/mL PBS onto Immulon-II HB 96-well ELISA plates (100 µL/well). The protein concentrations of these cell lysates were balanced, but not for the binding targets (Ags) that could be a big variation. Irrelevant mAb was used as an isotype control.

    Article Snippet: Indirect ELISA Ag (protein) (0.2 µg/mL in PBS) was coated onto Immulon-II HB 96-well ELISA plates (Thermo Fisher Scientific, Waltham, MA, USA) and incubated in a wet-box overnight at room temperature (RT).

    Techniques: Binding Assay, Indirect ELISA, Enzyme-linked Immunosorbent Assay