96 well flat bottomed microtiter plates  (Thermo Fisher)


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    Name:
    96 Well Plate Individual
    Description:
    Thermo Scientific Abgene polypropylene microplates and deepwell plates offer storage security for assays compound libraries or storing samples for either intermediate or long term use Abgene microtiter plates are manufactured to exacting specifications in our Class 100 000 clean room ISO 9001 conditions using high quality medical grade virgin polypropylene resins which ensure confidence in the quality and performance of the storage plates Designed to ANSI standards these microplates and deepwell plates are compatible with a variety of automated liquid handling for high throughput workflows The polypropylene storage plates are offered with spindle pyramidal U and V bottom wells along with a wide range of volumes to accommodate most applications
    Catalog Number:
    ab0765
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    Laboratory Plastics and Supplies|Microplates, Dishes and Flasks
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    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher 96 well flat bottomed microtiter plates
    Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in <t>96-well</t> flat-bottomed <t>microtiter</t> plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.
    Thermo Scientific Abgene polypropylene microplates and deepwell plates offer storage security for assays compound libraries or storing samples for either intermediate or long term use Abgene microtiter plates are manufactured to exacting specifications in our Class 100 000 clean room ISO 9001 conditions using high quality medical grade virgin polypropylene resins which ensure confidence in the quality and performance of the storage plates Designed to ANSI standards these microplates and deepwell plates are compatible with a variety of automated liquid handling for high throughput workflows The polypropylene storage plates are offered with spindle pyramidal U and V bottom wells along with a wide range of volumes to accommodate most applications
    https://www.bioz.com/result/96 well flat bottomed microtiter plates/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    96 well flat bottomed microtiter plates - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP"

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    Journal: Oncotarget

    doi: 10.18632/oncotarget.16703

    Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.
    Figure Legend Snippet: Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.

    Techniques Used: Imaging

    Vac leads to caspase 3/7 activation in glioma cells, counter regulated by ATP Caspase 3/7 activity given as relative color units (RCU) per μM 2 /image. Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control; 7 μM Vac; Vac+ATP 1 mM; and 1 mM ATP alone. Caspase 3/7 activity was monitored over 16 h (x-axis) (IncuCyteZOOM®) by adding IncuCyte® Caspase-3/7 reagent (y-axis, RCU×μM 2 /image) (A) . Quantitative analysis at 2 h for glioma cells (* in A). Vac vs. control and Vac vs. Vac+ATP (both p
    Figure Legend Snippet: Vac leads to caspase 3/7 activation in glioma cells, counter regulated by ATP Caspase 3/7 activity given as relative color units (RCU) per μM 2 /image. Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control; 7 μM Vac; Vac+ATP 1 mM; and 1 mM ATP alone. Caspase 3/7 activity was monitored over 16 h (x-axis) (IncuCyteZOOM®) by adding IncuCyte® Caspase-3/7 reagent (y-axis, RCU×μM 2 /image) (A) . Quantitative analysis at 2 h for glioma cells (* in A). Vac vs. control and Vac vs. Vac+ATP (both p

    Techniques Used: Activation Assay, Activity Assay

    Vac- vs STS-mediated cell death Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 2×10 −3 DMSO diluted in medium served as control, 7 μM Vac or 1 μM STS. Cell death was monitored over 20 h (x-axis) by PI staining (y-axis, RCU×μM 2 /image). All values are given as means ± SD (8× replicates) (A) . All imaging was performed using IncuCyteZOOM® at 10× objective. Caspase 3/7 activity was determined by luminescence assay (RLU, y-axis by Caspase Glo 3/7 assay, Promega.com ) after 4 h (*, A); control vs. Vac (t-test, p=0.0002); control vs. STS (t-test, p
    Figure Legend Snippet: Vac- vs STS-mediated cell death Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 2×10 −3 DMSO diluted in medium served as control, 7 μM Vac or 1 μM STS. Cell death was monitored over 20 h (x-axis) by PI staining (y-axis, RCU×μM 2 /image). All values are given as means ± SD (8× replicates) (A) . All imaging was performed using IncuCyteZOOM® at 10× objective. Caspase 3/7 activity was determined by luminescence assay (RLU, y-axis by Caspase Glo 3/7 assay, Promega.com ) after 4 h (*, A); control vs. Vac (t-test, p=0.0002); control vs. STS (t-test, p

    Techniques Used: Staining, Imaging, Activity Assay, Luminescence Assay, Caspase-Glo Assay, T-Test

    ATP concentrations interfering with Vac-induced cell death, inhibition by purinergic receptor inhibitors Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 7 μM Vac with or without 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM ATP, or DMSO diluted in medium at 10 −4 served as control. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 17 h (x-axis). All values are means of PI fluorescence ± SD (triplicate values) (A) . Vac-induced cell death (7 μM) (y-axis, RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the universal purinergic inhibitor Suramin (30 μM). All values are means of PI fluorescence ± SD (triplicate values) (B) . Vac-induced cell death (7 μM) (y-axis: RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the selective P2×7 inhibitor A-438079 (100 μM). All values are means of PI fluorescence ± SD (6× replicates) (C) . All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 2 independent experiments.
    Figure Legend Snippet: ATP concentrations interfering with Vac-induced cell death, inhibition by purinergic receptor inhibitors Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 7 μM Vac with or without 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM ATP, or DMSO diluted in medium at 10 −4 served as control. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 17 h (x-axis). All values are means of PI fluorescence ± SD (triplicate values) (A) . Vac-induced cell death (7 μM) (y-axis, RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the universal purinergic inhibitor Suramin (30 μM). All values are means of PI fluorescence ± SD (triplicate values) (B) . Vac-induced cell death (7 μM) (y-axis: RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the selective P2×7 inhibitor A-438079 (100 μM). All values are means of PI fluorescence ± SD (6× replicates) (C) . All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 2 independent experiments.

    Techniques Used: Inhibition, Fluorescence, Imaging

    2) Product Images from "4-1BB Costimulatory Signals Preferentially Induce CD8+ T Cell Proliferation and Lead to the Amplification In Vivo of Cytotoxic T Cell Responses"

    Article Title: 4-1BB Costimulatory Signals Preferentially Induce CD8+ T Cell Proliferation and Lead to the Amplification In Vivo of Cytotoxic T Cell Responses

    Journal: The Journal of Experimental Medicine

    doi:

    Anti–4-1BB mAbs costimulate anti-CD3–induced T cell proliferation. BALB/c T cells were cultured for 72 h at 2 × 10 5 /well in 96-well flat-bottomed microtiter plates with the indicated concentrations of 145.2C11 with PBS control (-•-) or with the following: anti–4-1BB mAbs 15B9 (-□-), 1D8 (--○--), 21E5 (--▴--), 3B8 (...•...), 3H3 (.-♦.-), and 3E1 (..-▿-..), each at 10 μg/ ml. Results are expressed as the mean ± SD of triplicate wells.
    Figure Legend Snippet: Anti–4-1BB mAbs costimulate anti-CD3–induced T cell proliferation. BALB/c T cells were cultured for 72 h at 2 × 10 5 /well in 96-well flat-bottomed microtiter plates with the indicated concentrations of 145.2C11 with PBS control (-•-) or with the following: anti–4-1BB mAbs 15B9 (-□-), 1D8 (--○--), 21E5 (--▴--), 3B8 (...•...), 3H3 (.-♦.-), and 3E1 (..-▿-..), each at 10 μg/ ml. Results are expressed as the mean ± SD of triplicate wells.

    Techniques Used: Cell Culture

    ( A ) Anti–4-1BB mAbs differentially costimulate in vitro proliferation of CD4 + and CD8 + T cell subsets. Purified resting CD4 + or CD8 + BALB/c splenic T cells were cultured for 72 h in 96-well microtiter plates at 1 × 10 5 /well with 0.5% APC ± anti– 4-1BB mAb at 10 μg/ml and the indicated concentration of anti-CD3 mAb. Cells were pulsed with 0.5 μCi/well [ 3 H]thymidine for the final 12 h of culture, harvested, and counted by liquid scintillation spectroscopy. ( B ) 4-1BB–Ig fusion protein inhibits anti-CD3– induced T cell proliferation. CD4 + and CD8 + T cells with APC were stimulated with anti-CD3 mAb at 300 ng/ml in the absence or presence of soluble 4-1BB–Ig fusion protein. Cells were pulsed during the final 12 h of culture with [ 3 H]thymidine, harvested, and counted by liquid scintillation spectroscopy.
    Figure Legend Snippet: ( A ) Anti–4-1BB mAbs differentially costimulate in vitro proliferation of CD4 + and CD8 + T cell subsets. Purified resting CD4 + or CD8 + BALB/c splenic T cells were cultured for 72 h in 96-well microtiter plates at 1 × 10 5 /well with 0.5% APC ± anti– 4-1BB mAb at 10 μg/ml and the indicated concentration of anti-CD3 mAb. Cells were pulsed with 0.5 μCi/well [ 3 H]thymidine for the final 12 h of culture, harvested, and counted by liquid scintillation spectroscopy. ( B ) 4-1BB–Ig fusion protein inhibits anti-CD3– induced T cell proliferation. CD4 + and CD8 + T cells with APC were stimulated with anti-CD3 mAb at 300 ng/ml in the absence or presence of soluble 4-1BB–Ig fusion protein. Cells were pulsed during the final 12 h of culture with [ 3 H]thymidine, harvested, and counted by liquid scintillation spectroscopy.

    Techniques Used: In Vitro, Purification, Cell Culture, Concentration Assay, Spectroscopy

    Related Articles

    Neutralization:

    Article Title: Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
    Article Snippet: .. SARS-CoV-2 Neutralization Assay The day prior to infection VeroE6 cells were seeded at 1×104 cells/well into 96-well plates. .. Plasma samples and antibodies were serial diluted in BA-1, consisting of medium 199 (Lonza, Inc.) supplemented with 1% bovine serum albumin (BSA) and 1x penicillin/streptomycin.

    Construct:

    Article Title: Different categories of fluorescent proteins result in GEVIs with similar characteristics
    Article Snippet: .. In all cases, for transient expression of GEVI constructs we used 0.1 µg per 96-well or 0.4 µg per 12 mm coverslip of DNA, and 0.25 µL per 96-well or 1 µL per per 12 mm coverslip of Lipofectamine2000 (Invitrogen). .. The experiments with the both lines of HEK293 cells were performed 24-48h post transfection.

    Evaporation:

    Article Title: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease
    Article Snippet: .. Phospholipid ELISA assay A PolySorp, 96-well ELISA plate (Thermo Scientific) was coated with a mixture of phospholipids dissolved in methanol in a final amount of 100 μg/well and incubated overnight at 4 °C for complete evaporation of methanol. .. Blocking was performed with 100 μl/well of 1% BSA (fatty acid-free) in PBS for one hour at 37 °C, followed by one wash with PBS.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease
    Article Snippet: .. Phospholipid ELISA assay A PolySorp, 96-well ELISA plate (Thermo Scientific) was coated with a mixture of phospholipids dissolved in methanol in a final amount of 100 μg/well and incubated overnight at 4 °C for complete evaporation of methanol. .. Blocking was performed with 100 μl/well of 1% BSA (fatty acid-free) in PBS for one hour at 37 °C, followed by one wash with PBS.

    Incubation:

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: .. Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

    Article Title: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease
    Article Snippet: .. Phospholipid ELISA assay A PolySorp, 96-well ELISA plate (Thermo Scientific) was coated with a mixture of phospholipids dissolved in methanol in a final amount of 100 μg/well and incubated overnight at 4 °C for complete evaporation of methanol. .. Blocking was performed with 100 μl/well of 1% BSA (fatty acid-free) in PBS for one hour at 37 °C, followed by one wash with PBS.

    Activity Assay:

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. The antifungal activity against C. albicans biofilms was determined in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. Cells (105 ) in 100 μL 0.05× PDB were distributed per well and plates were incubated at 37°C for up to 24 h to allow biofilm formation.

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. Antifungal Activity Determination The MIC of all protein and peptide variants was determined on C. albicans with broth microdilution assays in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. From a fresh C. albicans overnight culture on YPD agar, cells were harvested with an inoculation loop and suspended in 0.05× PDB (Sigma-Aldrich) ( ).

    Infection:

    Article Title: Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
    Article Snippet: .. SARS-CoV-2 Neutralization Assay The day prior to infection VeroE6 cells were seeded at 1×104 cells/well into 96-well plates. .. Plasma samples and antibodies were serial diluted in BA-1, consisting of medium 199 (Lonza, Inc.) supplemented with 1% bovine serum albumin (BSA) and 1x penicillin/streptomycin.

    Expressing:

    Article Title: Different categories of fluorescent proteins result in GEVIs with similar characteristics
    Article Snippet: .. In all cases, for transient expression of GEVI constructs we used 0.1 µg per 96-well or 0.4 µg per 12 mm coverslip of DNA, and 0.25 µL per 96-well or 1 µL per per 12 mm coverslip of Lipofectamine2000 (Invitrogen). .. The experiments with the both lines of HEK293 cells were performed 24-48h post transfection.

    Proliferation Assay:

    Article Title: ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells
    Article Snippet: .. For the proliferation assay shown in , control and ASH2L depleted L1236 cells were plated in 96-well plates at 1000 cells per well. ..

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  • 99
    Thermo Fisher 96 well flat bottomed microtiter plates
    Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in <t>96-well</t> flat-bottomed <t>microtiter</t> plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.
    96 Well Flat Bottomed Microtiter Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well flat bottomed microtiter plates/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    96 well flat bottomed microtiter plates - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Imaging

    Vac leads to caspase 3/7 activation in glioma cells, counter regulated by ATP Caspase 3/7 activity given as relative color units (RCU) per μM 2 /image. Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control; 7 μM Vac; Vac+ATP 1 mM; and 1 mM ATP alone. Caspase 3/7 activity was monitored over 16 h (x-axis) (IncuCyteZOOM®) by adding IncuCyte® Caspase-3/7 reagent (y-axis, RCU×μM 2 /image) (A) . Quantitative analysis at 2 h for glioma cells (* in A). Vac vs. control and Vac vs. Vac+ATP (both p

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: Vac leads to caspase 3/7 activation in glioma cells, counter regulated by ATP Caspase 3/7 activity given as relative color units (RCU) per μM 2 /image. Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control; 7 μM Vac; Vac+ATP 1 mM; and 1 mM ATP alone. Caspase 3/7 activity was monitored over 16 h (x-axis) (IncuCyteZOOM®) by adding IncuCyte® Caspase-3/7 reagent (y-axis, RCU×μM 2 /image) (A) . Quantitative analysis at 2 h for glioma cells (* in A). Vac vs. control and Vac vs. Vac+ATP (both p

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Activation Assay, Activity Assay

    Vac- vs STS-mediated cell death Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 2×10 −3 DMSO diluted in medium served as control, 7 μM Vac or 1 μM STS. Cell death was monitored over 20 h (x-axis) by PI staining (y-axis, RCU×μM 2 /image). All values are given as means ± SD (8× replicates) (A) . All imaging was performed using IncuCyteZOOM® at 10× objective. Caspase 3/7 activity was determined by luminescence assay (RLU, y-axis by Caspase Glo 3/7 assay, Promega.com ) after 4 h (*, A); control vs. Vac (t-test, p=0.0002); control vs. STS (t-test, p

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: Vac- vs STS-mediated cell death Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 2×10 −3 DMSO diluted in medium served as control, 7 μM Vac or 1 μM STS. Cell death was monitored over 20 h (x-axis) by PI staining (y-axis, RCU×μM 2 /image). All values are given as means ± SD (8× replicates) (A) . All imaging was performed using IncuCyteZOOM® at 10× objective. Caspase 3/7 activity was determined by luminescence assay (RLU, y-axis by Caspase Glo 3/7 assay, Promega.com ) after 4 h (*, A); control vs. Vac (t-test, p=0.0002); control vs. STS (t-test, p

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Staining, Imaging, Activity Assay, Luminescence Assay, Caspase-Glo Assay, T-Test

    ATP concentrations interfering with Vac-induced cell death, inhibition by purinergic receptor inhibitors Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 7 μM Vac with or without 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM ATP, or DMSO diluted in medium at 10 −4 served as control. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 17 h (x-axis). All values are means of PI fluorescence ± SD (triplicate values) (A) . Vac-induced cell death (7 μM) (y-axis, RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the universal purinergic inhibitor Suramin (30 μM). All values are means of PI fluorescence ± SD (triplicate values) (B) . Vac-induced cell death (7 μM) (y-axis: RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the selective P2×7 inhibitor A-438079 (100 μM). All values are means of PI fluorescence ± SD (6× replicates) (C) . All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 2 independent experiments.

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: ATP concentrations interfering with Vac-induced cell death, inhibition by purinergic receptor inhibitors Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 7 μM Vac with or without 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM ATP, or DMSO diluted in medium at 10 −4 served as control. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 17 h (x-axis). All values are means of PI fluorescence ± SD (triplicate values) (A) . Vac-induced cell death (7 μM) (y-axis, RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the universal purinergic inhibitor Suramin (30 μM). All values are means of PI fluorescence ± SD (triplicate values) (B) . Vac-induced cell death (7 μM) (y-axis: RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the selective P2×7 inhibitor A-438079 (100 μM). All values are means of PI fluorescence ± SD (6× replicates) (C) . All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 2 independent experiments.

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Inhibition, Fluorescence, Imaging