96 well flat bottom uv transparent plate  (Thermo Fisher)


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    Thermo Fisher 96 well flat bottom uv transparent plate
    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values are a comparison between treated vs. untreated cells ( n  = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph.  Abbreviations:  SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.
    96 Well Flat Bottom Uv Transparent Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well flat bottom uv transparent plate/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    96 well flat bottom uv transparent plate - by Bioz Stars, 2020-09
    92/100 stars

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    varioskan flash microplate reader

    Images

    1) Product Images from "Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core"

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    Journal: Nanomaterials

    doi: 10.3390/nano8080588

    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values are a comparison between treated vs. untreated cells ( n  = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph.  Abbreviations:  SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.
    Figure Legend Snippet: Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Techniques Used:

    Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values vs. untreated cells ( n  = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured.  Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.
    Figure Legend Snippet: Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values vs. untreated cells ( n = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured. Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Techniques Used: Multiple Displacement Amplification

    2) Product Images from "Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core"

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    Journal: Nanomaterials

    doi: 10.3390/nano8080588

    Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values vs. untreated cells ( n  = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured.  Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.
    Figure Legend Snippet: Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values vs. untreated cells ( n = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured. Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Techniques Used: Multiple Displacement Amplification

    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values are a comparison between treated vs. untreated cells ( n  = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph.  Abbreviations:  SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.
    Figure Legend Snippet: Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Techniques Used:

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    Thermo Fisher 96 well flat bottom uv transparent plate
    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values are a comparison between treated vs. untreated cells ( n  = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph.  Abbreviations:  SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.
    96 Well Flat Bottom Uv Transparent Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well flat bottom uv transparent plate/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    96 well flat bottom uv transparent plate - by Bioz Stars, 2020-09
    92/100 stars
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    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values are a comparison between treated vs. untreated cells ( n  = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph.  Abbreviations:  SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Journal: Nanomaterials

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    doi: 10.3390/nano8080588

    Figure Lengend Snippet: Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Article Snippet: An amount of 100 μL of the dissolved precipitate was transferred in triplicate to a 96-well flat bottom UV-transparent plate and was measured using Thermo Scientific Varioskan Flash Microplate Reader.

    Techniques:

    Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values vs. untreated cells ( n  = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured.  Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Journal: Nanomaterials

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    doi: 10.3390/nano8080588

    Figure Lengend Snippet: Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values vs. untreated cells ( n = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured. Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Article Snippet: An amount of 100 μL of the dissolved precipitate was transferred in triplicate to a 96-well flat bottom UV-transparent plate and was measured using Thermo Scientific Varioskan Flash Microplate Reader.

    Techniques: Multiple Displacement Amplification

    Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values vs. untreated cells ( n  = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured.  Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Journal: Nanomaterials

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    doi: 10.3390/nano8080588

    Figure Lengend Snippet: Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values vs. untreated cells ( n = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured. Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Article Snippet: An amount of 100 μL of the dissolved precipitate was transferred in triplicate to a 96-well flat bottom UV-transparent plate and was measured using Thermo Scientific Varioskan Flash Microplate Reader.

    Techniques: Multiple Displacement Amplification

    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3  where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results.  Notes:  The control is represented by the spheroids that were untreated. Indicated  p -values are a comparison between treated vs. untreated cells ( n  = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph.  Abbreviations:  SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Journal: Nanomaterials

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    doi: 10.3390/nano8080588

    Figure Lengend Snippet: Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Article Snippet: An amount of 100 μL of the dissolved precipitate was transferred in triplicate to a 96-well flat bottom UV-transparent plate and was measured using Thermo Scientific Varioskan Flash Microplate Reader.

    Techniques: