96 well flat bottom plates  (Thermo Fisher)


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    Name:
    96 Well Plate Individual
    Description:
    Thermo Scientific Abgene polypropylene microplates and deepwell plates offer storage security for assays compound libraries or storing samples for either intermediate or long term use Abgene microtiter plates are manufactured to exacting specifications in our Class 100 000 clean room ISO 9001 conditions using high quality medical grade virgin polypropylene resins which ensure confidence in the quality and performance of the storage plates Designed to ANSI standards these microplates and deepwell plates are compatible with a variety of automated liquid handling for high throughput workflows The polypropylene storage plates are offered with spindle pyramidal U and V bottom wells along with a wide range of volumes to accommodate most applications
    Catalog Number:
    ab0765
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    Applications:
    Laboratory Plastics and Supplies|Microplates, Dishes and Flasks
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    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher 96 well flat bottom plates
    Effect of TPT on the proliferative response of bidirectional MLR and anti-CD3/CD28–stimulated CD4 + T cells. Allogeneic human PBMCs from 2 different donors (3 × 10 5 cells/well each) were cocultured in <t>96-well</t> flat-bottom plates in the presence
    Thermo Scientific Abgene polypropylene microplates and deepwell plates offer storage security for assays compound libraries or storing samples for either intermediate or long term use Abgene microtiter plates are manufactured to exacting specifications in our Class 100 000 clean room ISO 9001 conditions using high quality medical grade virgin polypropylene resins which ensure confidence in the quality and performance of the storage plates Designed to ANSI standards these microplates and deepwell plates are compatible with a variety of automated liquid handling for high throughput workflows The polypropylene storage plates are offered with spindle pyramidal U and V bottom wells along with a wide range of volumes to accommodate most applications
    https://www.bioz.com/result/96 well flat bottom plates/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    96 well flat bottom plates - by Bioz Stars, 2020-08
    99/100 stars

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    Images

    1) Product Images from "Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking"

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking

    Journal:

    doi: 10.1182/blood-2005-03-0854

    Effect of TPT on the proliferative response of bidirectional MLR and anti-CD3/CD28–stimulated CD4 + T cells. Allogeneic human PBMCs from 2 different donors (3 × 10 5 cells/well each) were cocultured in 96-well flat-bottom plates in the presence
    Figure Legend Snippet: Effect of TPT on the proliferative response of bidirectional MLR and anti-CD3/CD28–stimulated CD4 + T cells. Allogeneic human PBMCs from 2 different donors (3 × 10 5 cells/well each) were cocultured in 96-well flat-bottom plates in the presence

    Techniques Used:

    2) Product Images from "Identification and characterization of noncalcemic, tissue-selective, nonsecosteroidal vitamin D receptor modulators"

    Article Title: Identification and characterization of noncalcemic, tissue-selective, nonsecosteroidal vitamin D receptor modulators

    Journal: Journal of Clinical Investigation

    doi: 10.1172/JCI25901

    Nonsecosteroidal VDR ligands are potent agonists in keratinocytes and PBMCs. ( A ) LY2108491 and LY2109866 are potent inhibitors of keratinocyte proliferation. KerTr cells plated in 96-well plates were dosed with various concentrations of 1,25-(OH) 2 D 3 , LY2108491, or LY2109866 for 72 hours at 37°C before BrdU incorporation into DNA was analyzed as a measure of cell proliferation. Results (mean α SEM) of experiments performed in triplicate are shown. ( B ) Nonsecosteroidal VDR ligands are potent inducers of CYP24 gene expression in keratinocytes. TaqMan quantitative RT-PCR (Q-PCR) was performed on total RNA prepared from KerTr cells treated with various concentrations of 1,25-(OH) 2 D 3 , LY2108491, or LY2109866 for 24 hours. Levels of GAPDH mRNA were measured in all the samples, and the results were normalized and presented as fold induction (α SEM) compared with normalized CYP24 levels in vehicle-treated cells. ( C ) Nonsecosteroidal VDR ligands are efficacious in TPA- and PHA-activated PBMCs. Primary cells isolated from donors were stimulated with TPA (100 ng/ml) and PHA (25 μl/ml) and treated with vehicle or 100 nM each of 1,25-(OH) 2 D 3 , LY2108491, or LY2109866 for 24 hours. TaqMan Q-PCR was performed on RNA obtained from vehicle-treated or VDR ligand–treated samples, using primer pairs and probes for IL-2, IL-4, IL-10, GATA3, and GAPDH. The amount of IL-2, IL-4, IL-10, and GATA3 transcripts relative to GAPDH transcripts is shown as mean α SEM of quadruplicate experimes.
    Figure Legend Snippet: Nonsecosteroidal VDR ligands are potent agonists in keratinocytes and PBMCs. ( A ) LY2108491 and LY2109866 are potent inhibitors of keratinocyte proliferation. KerTr cells plated in 96-well plates were dosed with various concentrations of 1,25-(OH) 2 D 3 , LY2108491, or LY2109866 for 72 hours at 37°C before BrdU incorporation into DNA was analyzed as a measure of cell proliferation. Results (mean α SEM) of experiments performed in triplicate are shown. ( B ) Nonsecosteroidal VDR ligands are potent inducers of CYP24 gene expression in keratinocytes. TaqMan quantitative RT-PCR (Q-PCR) was performed on total RNA prepared from KerTr cells treated with various concentrations of 1,25-(OH) 2 D 3 , LY2108491, or LY2109866 for 24 hours. Levels of GAPDH mRNA were measured in all the samples, and the results were normalized and presented as fold induction (α SEM) compared with normalized CYP24 levels in vehicle-treated cells. ( C ) Nonsecosteroidal VDR ligands are efficacious in TPA- and PHA-activated PBMCs. Primary cells isolated from donors were stimulated with TPA (100 ng/ml) and PHA (25 μl/ml) and treated with vehicle or 100 nM each of 1,25-(OH) 2 D 3 , LY2108491, or LY2109866 for 24 hours. TaqMan Q-PCR was performed on RNA obtained from vehicle-treated or VDR ligand–treated samples, using primer pairs and probes for IL-2, IL-4, IL-10, GATA3, and GAPDH. The amount of IL-2, IL-4, IL-10, and GATA3 transcripts relative to GAPDH transcripts is shown as mean α SEM of quadruplicate experimes.

    Techniques Used: BrdU Incorporation Assay, Expressing, Quantitative RT-PCR, Polymerase Chain Reaction, Isolation

    3) Product Images from "Effect of azithromycin on a red complex polymicrobial biofilm"

    Article Title: Effect of azithromycin on a red complex polymicrobial biofilm

    Journal: Journal of Oral Microbiology

    doi: 10.1080/20002297.2017.1339579

    Effect of azithromycin up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Azithromycin at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h under anaerobic conditions. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.
    Figure Legend Snippet: Effect of azithromycin up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Azithromycin at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h under anaerobic conditions. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Techniques Used: Incubation

    Formation of mono- and polymicrobial biofilms in a 96-well plate model after 48 h of incubation at 37°C under anaerobic condition. Native bacterial growth with addition of uncultured growth medium and no antibiotic served as controls. Adherent biofilms were stained with 0.1% crystal violet and the optical density at AU 620 was measured. Data represent the mean AU 620 value of a minimum of three biological replicates.
    Figure Legend Snippet: Formation of mono- and polymicrobial biofilms in a 96-well plate model after 48 h of incubation at 37°C under anaerobic condition. Native bacterial growth with addition of uncultured growth medium and no antibiotic served as controls. Adherent biofilms were stained with 0.1% crystal violet and the optical density at AU 620 was measured. Data represent the mean AU 620 value of a minimum of three biological replicates.

    Techniques Used: Incubation, Staining

    Effects of azithromycin and amoxicillin + metronidazole (1:1 ratio) up to 5.0 mg/L on formation polymicrobial biofilms after 48 h of anaerobic incubation at 37°C in a 96-well plate model. Azithromycin and amoxicillin + metronidazole (1:1 ratio) at concentrations 0–100 mg/L were incubated with bacterial cultures. Data points represent the mean AU 620 value of a minimum of three biological replicates and the standard deviation. * p
    Figure Legend Snippet: Effects of azithromycin and amoxicillin + metronidazole (1:1 ratio) up to 5.0 mg/L on formation polymicrobial biofilms after 48 h of anaerobic incubation at 37°C in a 96-well plate model. Azithromycin and amoxicillin + metronidazole (1:1 ratio) at concentrations 0–100 mg/L were incubated with bacterial cultures. Data points represent the mean AU 620 value of a minimum of three biological replicates and the standard deviation. * p

    Techniques Used: Incubation, Standard Deviation

    Effect of amoxicillin + metronidazole up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Amoxicillin + metronidazole in a 1:1 ratio at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h at 37°C anaerobically. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.
    Figure Legend Snippet: Effect of amoxicillin + metronidazole up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Amoxicillin + metronidazole in a 1:1 ratio at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h at 37°C anaerobically. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Techniques Used: Incubation

    4) Product Images from "Effect of azithromycin on a red complex polymicrobial biofilm"

    Article Title: Effect of azithromycin on a red complex polymicrobial biofilm

    Journal: Journal of Oral Microbiology

    doi: 10.1080/20002297.2017.1339579

    Effect of azithromycin up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Azithromycin at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h under anaerobic conditions. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.
    Figure Legend Snippet: Effect of azithromycin up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Azithromycin at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h under anaerobic conditions. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Techniques Used: Incubation

    Formation of mono- and polymicrobial biofilms in a 96-well plate model after 48 h of incubation at 37°C under anaerobic condition. Native bacterial growth with addition of uncultured growth medium and no antibiotic served as controls. Adherent biofilms were stained with 0.1% crystal violet and the optical density at AU 620 was measured. Data represent the mean AU 620 value of a minimum of three biological replicates.
    Figure Legend Snippet: Formation of mono- and polymicrobial biofilms in a 96-well plate model after 48 h of incubation at 37°C under anaerobic condition. Native bacterial growth with addition of uncultured growth medium and no antibiotic served as controls. Adherent biofilms were stained with 0.1% crystal violet and the optical density at AU 620 was measured. Data represent the mean AU 620 value of a minimum of three biological replicates.

    Techniques Used: Incubation, Staining

    Effects of azithromycin and amoxicillin + metronidazole (1:1 ratio) up to 5.0 mg/L on formation polymicrobial biofilms after 48 h of anaerobic incubation at 37°C in a 96-well plate model. Azithromycin and amoxicillin + metronidazole (1:1 ratio) at concentrations 0–100 mg/L were incubated with bacterial cultures. Data points represent the mean AU 620 value of a minimum of three biological replicates and the standard deviation. * p
    Figure Legend Snippet: Effects of azithromycin and amoxicillin + metronidazole (1:1 ratio) up to 5.0 mg/L on formation polymicrobial biofilms after 48 h of anaerobic incubation at 37°C in a 96-well plate model. Azithromycin and amoxicillin + metronidazole (1:1 ratio) at concentrations 0–100 mg/L were incubated with bacterial cultures. Data points represent the mean AU 620 value of a minimum of three biological replicates and the standard deviation. * p

    Techniques Used: Incubation, Standard Deviation

    Effect of amoxicillin + metronidazole up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Amoxicillin + metronidazole in a 1:1 ratio at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h at 37°C anaerobically. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.
    Figure Legend Snippet: Effect of amoxicillin + metronidazole up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Amoxicillin + metronidazole in a 1:1 ratio at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h at 37°C anaerobically. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Techniques Used: Incubation

    5) Product Images from "Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge"

    Article Title: Intranasal Delivery of Cationic PLGA Nano/Microparticles- Loaded FMDV DNA Vaccine Encoding IL-6 Elicited Protective Immunity against FMDV Challenge

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0027605

    Lymphocyte proliferation. Single lymphocyte suspensions were isolated from rats (n = 6) 7 days after the second immunization, plated in triplicate in a 96-well plate and stimulated in vitro for 48 h with inactivated FMDV, Con A (positive control) or with BSA. Means were compared by non-parametric ANOVA. Proliferation was analyzed using the MTT colorimetric assay and proliferation expressed as stimulated index. Significant results between pA-immunized animals and control groups are indicated by #.
    Figure Legend Snippet: Lymphocyte proliferation. Single lymphocyte suspensions were isolated from rats (n = 6) 7 days after the second immunization, plated in triplicate in a 96-well plate and stimulated in vitro for 48 h with inactivated FMDV, Con A (positive control) or with BSA. Means were compared by non-parametric ANOVA. Proliferation was analyzed using the MTT colorimetric assay and proliferation expressed as stimulated index. Significant results between pA-immunized animals and control groups are indicated by #.

    Techniques Used: Isolation, In Vitro, Positive Control, MTT Assay, Colorimetric Assay

    6) Product Images from "Activated neutrophils exert myeloid-derived suppressor cell activity damaging T cells beyond repair"

    Article Title: Activated neutrophils exert myeloid-derived suppressor cell activity damaging T cells beyond repair

    Journal: Blood Advances

    doi: 10.1182/bloodadvances.2019031609

    Activated neutrophils suppress T-cell proliferation. Purified T cells (either CD4 + or CD8 + ) were cultured in the presence or absence of anti-CD3 antibody or anti-CD28 antibody with unstimulated or fMLF-activated neutrophils (unless otherwise indicated). Cells were harvested after 5 to 6 days and analyzed by flow cytometry for CFSE dilution. (A) Representative fluorescence-activated cell sorting (FACS) plots of CFSE dilution of CD4 + T cells. (B) Quantification of CD4 + (left) and CD8 + (right) T-cell proliferation (n = 17). (C) Titration of the cell ratio with 4000 (5:1 ratio), 20 000 (1:1), 40 000 (1:2), 60 000 (1:3), 100 000 (1:5), or 160 000 (1:8) neutrophils per well of a 96-well plate (n = 3-17). (D) Purified T cells were cultured in the presence or absence of IL-15 with unstimulated or fMLF-activated neutrophils (n = 5). (E) Purified T cells were cultured with anti-CD3 and anti-CD28 antibodies (red bars), and in the presence of neutrophils (blue bars) and/or indicated stimuli. Three to 19 donors were tested in duplicate per stimulus. Error bars indicate standard error of the mean (SEM); **** P
    Figure Legend Snippet: Activated neutrophils suppress T-cell proliferation. Purified T cells (either CD4 + or CD8 + ) were cultured in the presence or absence of anti-CD3 antibody or anti-CD28 antibody with unstimulated or fMLF-activated neutrophils (unless otherwise indicated). Cells were harvested after 5 to 6 days and analyzed by flow cytometry for CFSE dilution. (A) Representative fluorescence-activated cell sorting (FACS) plots of CFSE dilution of CD4 + T cells. (B) Quantification of CD4 + (left) and CD8 + (right) T-cell proliferation (n = 17). (C) Titration of the cell ratio with 4000 (5:1 ratio), 20 000 (1:1), 40 000 (1:2), 60 000 (1:3), 100 000 (1:5), or 160 000 (1:8) neutrophils per well of a 96-well plate (n = 3-17). (D) Purified T cells were cultured in the presence or absence of IL-15 with unstimulated or fMLF-activated neutrophils (n = 5). (E) Purified T cells were cultured with anti-CD3 and anti-CD28 antibodies (red bars), and in the presence of neutrophils (blue bars) and/or indicated stimuli. Three to 19 donors were tested in duplicate per stimulus. Error bars indicate standard error of the mean (SEM); **** P

    Techniques Used: Purification, Cell Culture, Flow Cytometry, Cytometry, Fluorescence, FACS, Titration

    7) Product Images from "Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral Cytotoxicity"

    Article Title: Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral Cytotoxicity

    Journal: Journal of Immunology Research

    doi: 10.1155/2016/9540975

    The luciferase IVT RNA-based assay efficiently assesses mAb-induced ADCC and CDC of tumour cell lines. ADCC assay using (a) KATO-III and (b) NUGC-4 cells. KATO-III and NUGC-4 cells endogenously expressing hCLDN18.2 were transfected with 7 μ g luc2 IVT RNA and seeded into 96-well plates independently. 4 h later, IMAB 362 at different concentrations and human PBMCs (E : T ratio = 40 : 1) from 6 different donors were added to the target cells and incubated for 24 h. ADCC was determined 40 and 45 min after addition of D-luciferin substrate to the KATO-III and NUGC-4 cells, respectively. (c) CDC assay. CHO-K1 cells stably expressing hCLDN18.2 were transfected with 7 μ g luc2 IVT RNA and seeded into 96-well plates. 24 h later, cells were incubated for 80 min with IMAB 362 diluted in human serum (final concentration of 20%) from 6 different healthy donors. CDC was determined 45 min after addition of D-luciferin substrate. Results are the mean ± SD ( n = 3).
    Figure Legend Snippet: The luciferase IVT RNA-based assay efficiently assesses mAb-induced ADCC and CDC of tumour cell lines. ADCC assay using (a) KATO-III and (b) NUGC-4 cells. KATO-III and NUGC-4 cells endogenously expressing hCLDN18.2 were transfected with 7 μ g luc2 IVT RNA and seeded into 96-well plates independently. 4 h later, IMAB 362 at different concentrations and human PBMCs (E : T ratio = 40 : 1) from 6 different donors were added to the target cells and incubated for 24 h. ADCC was determined 40 and 45 min after addition of D-luciferin substrate to the KATO-III and NUGC-4 cells, respectively. (c) CDC assay. CHO-K1 cells stably expressing hCLDN18.2 were transfected with 7 μ g luc2 IVT RNA and seeded into 96-well plates. 24 h later, cells were incubated for 80 min with IMAB 362 diluted in human serum (final concentration of 20%) from 6 different healthy donors. CDC was determined 45 min after addition of D-luciferin substrate. Results are the mean ± SD ( n = 3).

    Techniques Used: Luciferase, ADCC Assay, Expressing, Transfection, Incubation, CDC Assay, Stable Transfection, Concentration Assay

    Electroporation of firefly luciferase IVT RNA into DCs and K562-A2 cells is nontoxic and leads to strong and long-lasting gene expression without affecting target cell phenotype. (a) Optimised luc2 reporter vector: composed of a gene-optimized synthetic firefly luciferase reporter gene cloned in front of two human β -globin 3′ untranslated regions (UTRs) fused head to tail and an unmasked free poly(A) tail of 120 bp. (b) Kinetics of luc2 expression in K562-A2 cells ( n = 1), human iDCs ( n = 3), and mDCs ( n = 3). Cells transfected with 8 pmol of luc2 -encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 10 4 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μ g eGFP- or luc2 -encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no e'p) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 10 4 viable cells was measured by luminescence in triplicate.
    Figure Legend Snippet: Electroporation of firefly luciferase IVT RNA into DCs and K562-A2 cells is nontoxic and leads to strong and long-lasting gene expression without affecting target cell phenotype. (a) Optimised luc2 reporter vector: composed of a gene-optimized synthetic firefly luciferase reporter gene cloned in front of two human β -globin 3′ untranslated regions (UTRs) fused head to tail and an unmasked free poly(A) tail of 120 bp. (b) Kinetics of luc2 expression in K562-A2 cells ( n = 1), human iDCs ( n = 3), and mDCs ( n = 3). Cells transfected with 8 pmol of luc2 -encoding IVT RNA were harvested at different time points to measure luminescence from 1 × 10 4 cells (Bright-Glo Luciferase Assay Kit for 96-well plates (Promega)). Results are the mean ± SD luminescence. Percent luminescence is relative to the highest luminescence signal obtained in each experiment. (c) Viability, reporter gene expression of iDCs and mDCs after eGFP and luc2 electroporation and phenotype after electroporation are depicted in descending order, respectively. iDCs (left panel) and mDCs (right panel) of 2 different donors were transfected with 10 μ g eGFP- or luc2 -encoding IVT RNA. Negative controls: cells electroporated without RNA (mock) and unelectroporated (no e'p) cells. Cells were harvested at different time points. Viability and HLA-DR, CD83, and eGFP expression levels were determined by flow cytometry. Luciferase activity of 1 × 10 4 viable cells was measured by luminescence in triplicate.

    Techniques Used: Electroporation, Luciferase, Expressing, Plasmid Preparation, Clone Assay, Transfection, Flow Cytometry, Cytometry, Activity Assay

    8) Product Images from "Serum Amyloid P Component Bound to Gram-Negative Bacteria Prevents Lipopolysaccharide-Mediated Classical Pathway Complement Activation"

    Article Title: Serum Amyloid P Component Bound to Gram-Negative Bacteria Prevents Lipopolysaccharide-Mediated Classical Pathway Complement Activation

    Journal: Infection and Immunity

    doi:

    SAP inhibits the lysis of serovar Copenhagen Re by serum. Log phase-grown serovar Copenhagen Re was incubated overnight in HBSS–0.2% albumin–10% Mueller-Hinton broth in different concentrations of SAP − serum in the absence or presence of SAP (20 μg/ml) at 37°C in 96-well flat-bottom plates. The increase in optical density at 665 nm (OD 665 ) was used to determine bacterial growth. Data are means for three separate experiments ± SEM.
    Figure Legend Snippet: SAP inhibits the lysis of serovar Copenhagen Re by serum. Log phase-grown serovar Copenhagen Re was incubated overnight in HBSS–0.2% albumin–10% Mueller-Hinton broth in different concentrations of SAP − serum in the absence or presence of SAP (20 μg/ml) at 37°C in 96-well flat-bottom plates. The increase in optical density at 665 nm (OD 665 ) was used to determine bacterial growth. Data are means for three separate experiments ± SEM.

    Techniques Used: Lysis, Incubation

    Related Articles

    Irradiation:

    Article Title: Critical role of amino acid position 343 of surfactant protein-D in the selective binding of glycolipids from Mycobacterium tuberculosis
    Article Snippet: .. Bacterial single cell suspensions (5 × 105 ) in 50 μL TBS (50 mM Tris-hydrochloride + 150 mM sodium chloride, pH 7.5) were added and dried onto triplicate wells of a 96-well microtiter plate (Immulon 1; Thermo Electron Corporation, Milford, MA) followed by overnight UV irradiation treatment to kill the bacteria. .. To confirm that NCRD binding was independent of the UV exposure, ELISA experiments were also performed without UV treatment with no significant differences seen in the results (data not shown).

    Cell Culture:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: .. Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway). ..

    Purification:

    Article Title: Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking
    Article Snippet: .. Purified CD4+ T cells (105 /well) were cultured in 96-well flat-bottom plates with CD3/CD28 T-cell expansion Dynabeads (5 × 103 /well; Dynal ASA, Oslo, Norway). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease
    Article Snippet: .. Phospholipid ELISA assay A PolySorp, 96-well ELISA plate (Thermo Scientific) was coated with a mixture of phospholipids dissolved in methanol in a final amount of 100 μg/well and incubated overnight at 4 °C for complete evaporation of methanol. .. Blocking was performed with 100 μl/well of 1% BSA (fatty acid-free) in PBS for one hour at 37 °C, followed by one wash with PBS.

    Incubation:

    Article Title: Tumor cells and their crosstalk with endothelial cells in 3D spheroids
    Article Snippet: .. Spheroids formation using multi-well agarose-coated plates Agarose hydrogel 1.5% (100 µl) was added to each well of a 96-well culture plate (Thermo Fisher Scientific, Denmark), and incubated at 37 °C for 2 hours. .. A375, BxPC3, PANC1 and MDA-MB-231cells were seeded in 100 µl growth medium at a concentration of 5,000 cells per well.

    Article Title: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease
    Article Snippet: .. Phospholipid ELISA assay A PolySorp, 96-well ELISA plate (Thermo Scientific) was coated with a mixture of phospholipids dissolved in methanol in a final amount of 100 μg/well and incubated overnight at 4 °C for complete evaporation of methanol. .. Blocking was performed with 100 μl/well of 1% BSA (fatty acid-free) in PBS for one hour at 37 °C, followed by one wash with PBS.

    Activity Assay:

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. The antifungal activity against C. albicans biofilms was determined in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. Cells (105 ) in 100 μL 0.05× PDB were distributed per well and plates were incubated at 37°C for up to 24 h to allow biofilm formation.

    Article Title: The Evolutionary Conserved γ-Core Motif Influences the Anti-Candida Activity of the Penicillium chrysogenum Antifungal Protein PAF
    Article Snippet: .. Antifungal Activity Determination The MIC of all protein and peptide variants was determined on C. albicans with broth microdilution assays in 96-well microtiter plates (Nunclon Delta, Thermo Fisher Scientific). .. From a fresh C. albicans overnight culture on YPD agar, cells were harvested with an inoculation loop and suspended in 0.05× PDB (Sigma-Aldrich) ( ).

    Evaporation:

    Article Title: Total and Proteinase K-Resistant α-Synuclein Levels in Erythrocytes, Determined by their Ability to Bind Phospholipids, Associate with Parkinson’s Disease
    Article Snippet: .. Phospholipid ELISA assay A PolySorp, 96-well ELISA plate (Thermo Scientific) was coated with a mixture of phospholipids dissolved in methanol in a final amount of 100 μg/well and incubated overnight at 4 °C for complete evaporation of methanol. .. Blocking was performed with 100 μl/well of 1% BSA (fatty acid-free) in PBS for one hour at 37 °C, followed by one wash with PBS.

    Plasmid Preparation:

    Article Title: Inhibition of Pyrimidine Biosynthesis Pathway Suppresses Viral Growth through Innate Immunity
    Article Snippet: .. For one 96-well culture plate, 17 µg of plasmid were diluted in 500 µl of DMEM (Gibco-Invitrogen). .. In parallel, 53 µg of poly(ethyleneimine) from Sigma-Aldrich (PEI) were diluted in 500 µl of DMEM (Gibco-BRL).

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    • 96 well flat bottomed microtiter plates  (Thermo Fisher)
    99
    Thermo Fisher 96 well flat bottomed microtiter plates
    Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in <t>96-well</t> flat-bottomed <t>microtiter</t> plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.
    96 Well Flat Bottomed Microtiter Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    96 well flat bottomed microtiter plates - by Bioz Stars, 2020-08
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    96 well flat bottom uv transparent plate  (Thermo Fisher)
    92
    Thermo Fisher 96 well flat bottom uv transparent plate
    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.
    96 Well Flat Bottom Uv Transparent Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: Carvacrol influences the ATP-recovery effect on Vac-induced cell death Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control, Vac (7 μM), Vac+ATP or ATP without carvacrol or with 50 μM and 100 μM carvacrol. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 24 h (x-axis) (IncuCyteZOOM®) (A, B) . Quantitative analysis at 8 h for glioma cell lines (* in A and B) (C) . Vac+ATP vs. Vac+ATP+carvacrol 100 μM (multiple comparison two way ANOVA, p=0.02). All values are given as means ± SD (6× replicates). All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 3 independent experiments.

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Imaging

    Vac leads to caspase 3/7 activation in glioma cells, counter regulated by ATP Caspase 3/7 activity given as relative color units (RCU) per μM 2 /image. Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control; 7 μM Vac; Vac+ATP 1 mM; and 1 mM ATP alone. Caspase 3/7 activity was monitored over 16 h (x-axis) (IncuCyteZOOM®) by adding IncuCyte® Caspase-3/7 reagent (y-axis, RCU×μM 2 /image) (A) . Quantitative analysis at 2 h for glioma cells (* in A). Vac vs. control and Vac vs. Vac+ATP (both p

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: Vac leads to caspase 3/7 activation in glioma cells, counter regulated by ATP Caspase 3/7 activity given as relative color units (RCU) per μM 2 /image. Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with DMSO diluted in medium at 10 −4 served as control; 7 μM Vac; Vac+ATP 1 mM; and 1 mM ATP alone. Caspase 3/7 activity was monitored over 16 h (x-axis) (IncuCyteZOOM®) by adding IncuCyte® Caspase-3/7 reagent (y-axis, RCU×μM 2 /image) (A) . Quantitative analysis at 2 h for glioma cells (* in A). Vac vs. control and Vac vs. Vac+ATP (both p

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Activation Assay, Activity Assay

    Vac- vs STS-mediated cell death Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 2×10 −3 DMSO diluted in medium served as control, 7 μM Vac or 1 μM STS. Cell death was monitored over 20 h (x-axis) by PI staining (y-axis, RCU×μM 2 /image). All values are given as means ± SD (8× replicates) (A) . All imaging was performed using IncuCyteZOOM® at 10× objective. Caspase 3/7 activity was determined by luminescence assay (RLU, y-axis by Caspase Glo 3/7 assay, Promega.com ) after 4 h (*, A); control vs. Vac (t-test, p=0.0002); control vs. STS (t-test, p

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: Vac- vs STS-mediated cell death Semi-confluent glioma cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 2×10 −3 DMSO diluted in medium served as control, 7 μM Vac or 1 μM STS. Cell death was monitored over 20 h (x-axis) by PI staining (y-axis, RCU×μM 2 /image). All values are given as means ± SD (8× replicates) (A) . All imaging was performed using IncuCyteZOOM® at 10× objective. Caspase 3/7 activity was determined by luminescence assay (RLU, y-axis by Caspase Glo 3/7 assay, Promega.com ) after 4 h (*, A); control vs. Vac (t-test, p=0.0002); control vs. STS (t-test, p

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Staining, Imaging, Activity Assay, Luminescence Assay, Caspase-Glo Assay, T-Test

    ATP concentrations interfering with Vac-induced cell death, inhibition by purinergic receptor inhibitors Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 7 μM Vac with or without 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM ATP, or DMSO diluted in medium at 10 −4 served as control. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 17 h (x-axis). All values are means of PI fluorescence ± SD (triplicate values) (A) . Vac-induced cell death (7 μM) (y-axis, RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the universal purinergic inhibitor Suramin (30 μM). All values are means of PI fluorescence ± SD (triplicate values) (B) . Vac-induced cell death (7 μM) (y-axis: RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the selective P2×7 inhibitor A-438079 (100 μM). All values are means of PI fluorescence ± SD (6× replicates) (C) . All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 2 independent experiments.

    Journal: Oncotarget

    Article Title: Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

    doi: 10.18632/oncotarget.16703

    Figure Lengend Snippet: ATP concentrations interfering with Vac-induced cell death, inhibition by purinergic receptor inhibitors Semi-confluent #12537-GB cells (seeded in 96-well flat-bottomed microtiter plates) were treated with 7 μM Vac with or without 10 nM, 100 nM, 1 μM, 10 μM, 100 μM and 1 mM ATP, or DMSO diluted in medium at 10 −4 served as control. PI-positive (dead) cells are given as RCU (y-axis, RCU×μM 2 /image). Glioma cells were followed for 17 h (x-axis). All values are means of PI fluorescence ± SD (triplicate values) (A) . Vac-induced cell death (7 μM) (y-axis, RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the universal purinergic inhibitor Suramin (30 μM). All values are means of PI fluorescence ± SD (triplicate values) (B) . Vac-induced cell death (7 μM) (y-axis: RCU×μM 2 /image) recorded for 24 h (x-axis) was attenuated by ATP (1 mM) but was further increased by ATP in the presence of the selective P2×7 inhibitor A-438079 (100 μM). All values are means of PI fluorescence ± SD (6× replicates) (C) . All imaging was performed using IncuCyteZOOM® at 10× objective. Results are representative of 2 independent experiments.

    Article Snippet: Semi-confluent cell layers seeded into 96-well flat-bottomed microtiter plates ( ThermoFisher.com ) were treated with a final concentration of 7 μM Vac in the presence of 10 μg/ml PI ( Sigma.com ) with and without ATP, carvacrol ( Sigma.com ), suramin ( Tocris/Biotechné.com , UK) or A-438079 ( Sellekchem.com ).

    Techniques: Inhibition, Fluorescence, Imaging

    Effect of azithromycin up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Azithromycin at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h under anaerobic conditions. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Journal: Journal of Oral Microbiology

    Article Title: Effect of azithromycin on a red complex polymicrobial biofilm

    doi: 10.1080/20002297.2017.1339579

    Figure Lengend Snippet: Effect of azithromycin up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Azithromycin at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h under anaerobic conditions. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Article Snippet: Two hundred microliters of P. gingivalis , T. denticola , or T. forsythia as a monospecies inoculum and the combination of each two bacterial species at equal volumes (100 µL each), as well as all three species (67 µL each) as a polymicrobial inoculum, were aliquoted into 96-well flat-bottom plates (Nunc; Thermo Scientific) to provide the same total number of bacterial cells per inoculum.

    Techniques: Incubation

    Formation of mono- and polymicrobial biofilms in a 96-well plate model after 48 h of incubation at 37°C under anaerobic condition. Native bacterial growth with addition of uncultured growth medium and no antibiotic served as controls. Adherent biofilms were stained with 0.1% crystal violet and the optical density at AU 620 was measured. Data represent the mean AU 620 value of a minimum of three biological replicates.

    Journal: Journal of Oral Microbiology

    Article Title: Effect of azithromycin on a red complex polymicrobial biofilm

    doi: 10.1080/20002297.2017.1339579

    Figure Lengend Snippet: Formation of mono- and polymicrobial biofilms in a 96-well plate model after 48 h of incubation at 37°C under anaerobic condition. Native bacterial growth with addition of uncultured growth medium and no antibiotic served as controls. Adherent biofilms were stained with 0.1% crystal violet and the optical density at AU 620 was measured. Data represent the mean AU 620 value of a minimum of three biological replicates.

    Article Snippet: Two hundred microliters of P. gingivalis , T. denticola , or T. forsythia as a monospecies inoculum and the combination of each two bacterial species at equal volumes (100 µL each), as well as all three species (67 µL each) as a polymicrobial inoculum, were aliquoted into 96-well flat-bottom plates (Nunc; Thermo Scientific) to provide the same total number of bacterial cells per inoculum.

    Techniques: Incubation, Staining

    Effects of azithromycin and amoxicillin + metronidazole (1:1 ratio) up to 5.0 mg/L on formation polymicrobial biofilms after 48 h of anaerobic incubation at 37°C in a 96-well plate model. Azithromycin and amoxicillin + metronidazole (1:1 ratio) at concentrations 0–100 mg/L were incubated with bacterial cultures. Data points represent the mean AU 620 value of a minimum of three biological replicates and the standard deviation. * p

    Journal: Journal of Oral Microbiology

    Article Title: Effect of azithromycin on a red complex polymicrobial biofilm

    doi: 10.1080/20002297.2017.1339579

    Figure Lengend Snippet: Effects of azithromycin and amoxicillin + metronidazole (1:1 ratio) up to 5.0 mg/L on formation polymicrobial biofilms after 48 h of anaerobic incubation at 37°C in a 96-well plate model. Azithromycin and amoxicillin + metronidazole (1:1 ratio) at concentrations 0–100 mg/L were incubated with bacterial cultures. Data points represent the mean AU 620 value of a minimum of three biological replicates and the standard deviation. * p

    Article Snippet: Two hundred microliters of P. gingivalis , T. denticola , or T. forsythia as a monospecies inoculum and the combination of each two bacterial species at equal volumes (100 µL each), as well as all three species (67 µL each) as a polymicrobial inoculum, were aliquoted into 96-well flat-bottom plates (Nunc; Thermo Scientific) to provide the same total number of bacterial cells per inoculum.

    Techniques: Incubation, Standard Deviation

    Effect of amoxicillin + metronidazole up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Amoxicillin + metronidazole in a 1:1 ratio at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h at 37°C anaerobically. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Journal: Journal of Oral Microbiology

    Article Title: Effect of azithromycin on a red complex polymicrobial biofilm

    doi: 10.1080/20002297.2017.1339579

    Figure Lengend Snippet: Effect of amoxicillin + metronidazole up to 5.0 mg/L on the red complex mono- and polymicrobial biofilms in a 96-well plate model. Amoxicillin + metronidazole in a 1:1 ratio at concentrations 0–100 mg/L was incubated with bacterial cultures for 48 h at 37°C anaerobically. Data points represent the mean AU 620 value of a minimum of three biological replicates. Note the categorical scale.

    Article Snippet: Two hundred microliters of P. gingivalis , T. denticola , or T. forsythia as a monospecies inoculum and the combination of each two bacterial species at equal volumes (100 µL each), as well as all three species (67 µL each) as a polymicrobial inoculum, were aliquoted into 96-well flat-bottom plates (Nunc; Thermo Scientific) to provide the same total number of bacterial cells per inoculum.

    Techniques: Incubation

    Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Journal: Nanomaterials

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    doi: 10.3390/nano8080588

    Figure Lengend Snippet: Changes in PANC-1 cell aggregate volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for cell aggregate formation followed by 3 days of treatment). ( B ) Cell aggregate volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Cell aggregate volume shown in the graph represents a cell aggregate volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values are a comparison between treated vs. untreated cells ( n = 26 cell aggregates). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not a complete representation of all the spheroids that were measured and plotted in the bar graph. Abbreviations: SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin; CA, cell aggregate.

    Article Snippet: An amount of 100 μL of the dissolved precipitate was transferred in triplicate to a 96-well flat bottom UV-transparent plate and was measured using Thermo Scientific Varioskan Flash Microplate Reader.

    Techniques:

    Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values vs. untreated cells ( n = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured. Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Journal: Nanomaterials

    Article Title: Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core

    doi: 10.3390/nano8080588

    Figure Lengend Snippet: Changes in MDA-MB231 spheroid volume following exposure to 3 μM empty and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. ( A ) Phase contrast images at 4× objective of changes in spheroid volume over the course of three days following exposure to 3 μM unloaded and curcumin loaded SMA and FA-DABA-SMA and 3 μM curcumin only. A total of 10,000 cells were plated per well in a 96 well plate for a total of 7 days (4 days for spheroid formation followed by 3 days of treatment). ( B ) Spheroid volume was measured daily following treatment using V = (4/3) πr 3 where π = 3.1415 and r = average radius (μm). Radius was measured using a scale bar. Spheroid volume shown in the graph represent spheroid volume ± standard error (error bars). Results were compared by a one-way ANOVA at 95% confidence using Fisher’s LSD test. The data presented in the graph are the combined results of two independent experiments that showed similar results. Notes: The control is represented by the spheroids that were untreated. Indicated p -values vs. untreated cells ( n = 38). The image scale bar represents 100 μm. The insets are magnified 300%. Phase contrast images are not the complete representation of all the spheroids that were measured. Abbreviations : SMA, poly(styrene- alt -maleic anhydride); FA, folic acid; DABA, 2,4-diaminobutyric acid; Cur, curcumin.

    Article Snippet: An amount of 100 μL of the dissolved precipitate was transferred in triplicate to a 96-well flat bottom UV-transparent plate and was measured using Thermo Scientific Varioskan Flash Microplate Reader.

    Techniques: Multiple Displacement Amplification