clear flat bottom immuno nonsterile 96 well plates  (Thermo Fisher)


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    Name:
    Clear Flat Bottom Immuno Nonsterile 96 Well Plates
    Description:
    Choose from four binding surfaces for reliable and reproducible results in colormetric assays with Thermo Scientific Clear Flat Bottom Immuno Nonsterile 96 Well Plates which feature Thermo Scientific Nunc Immulon and Microtiter clear plates that are ideal for quantitative and qualitative solid phase immunoassays
    Catalog Number:
    3355
    Price:
    None
    Applications:
    Clinical|Diagnostic Development
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    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher clear flat bottom immuno nonsterile 96 well plates
    Choose from four binding surfaces for reliable and reproducible results in colormetric assays with Thermo Scientific Clear Flat Bottom Immuno Nonsterile 96 Well Plates which feature Thermo Scientific Nunc Immulon and Microtiter clear plates that are ideal for quantitative and qualitative solid phase immunoassays
    https://www.bioz.com/result/clear flat bottom immuno nonsterile 96 well plates/product/Thermo Fisher
    Average 90 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    clear flat bottom immuno nonsterile 96 well plates - by Bioz Stars, 2020-01
    90/100 stars

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    Centrifugation:

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: Soluble proteins were recovered in the supernatant after centrifugation. .. The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6).

    Blocking Assay:

    Article Title: “Glowing Head” Mice: A Genetic Tool Enabling Reliable Preclinical Image-Based Evaluation of Cancers in Immunocompetent Allografts
    Article Snippet: To analyze anti-GFP antibody in serum, ELISA plates (Nunc MaxiSorp, cat# 439454, Thermo Scientific, Waltham, MA, USA) were coated with 31.25 ng of recombinant GFP (MB-0752, Vector Laboratory, Burlingame, CA, USA) in each well overnight at 4°C. .. The next day, sera and control monoclonal anti-GFP antibody (11814460001, Roche Applied Science, Indianapolis, IN, USA) were subjected to serial dilution with blocking solution (3% milk in phosphate-buffered saline [PBS]) to reach the range 1∶25–1∶2000 for the former and 6.25–200 ng/ml for the latter.

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6). .. After overnight incubation, the plates were washed (1× PBS, 0.05% Tween 20) and incubated for 1 h at 37°C with 250 μl of blocking buffer (2% bovine serum albumin [BSA], 1× PBS, 0.05% Tween 20) per well.

    Article Title: An ELISA Assay for Quantifying Monomeric C-Reactive Protein in Plasma
    Article Snippet: ELISA Assay Quantifying mCRP The mouse antihuman CRP mAb CRP-8 (Sigma-Aldrich, St. Louis, MO, USA; catalog number: C1688; lot number: 025M4863V) was immobilized onto microtiter wells (Thermo Fisher Scientific; catalog number: 468667, 442404; lot number: 148860, 148034; Corning, NY, USA; catalog number: 42592; lot number: 10917007) at 1:1,000 in coating buffer (10-mM sodium carbonate/bicarbonate, pH 9.6) overnight at 4°C. .. Wells were washed with TBS containing 0.02% NP-40, and then blocked with 1% BSA in TBS (blocking buffer).

    Article Title: Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model
    Article Snippet: Human and mouse CD1d, hCD1dmu and muCD1dhu were coated on 96-well NuncTM MaxisorpTM ELISA Plates (ThermoFisher, 442404) at 1 μg/mL in PBS overnight at 4°C. .. The plates were blocked for 1 h at room temperature in blocking buffer (PBS + 1% BSA) before being washed 3 times.

    Article Title: An ELISA Assay for Quantifying Monomeric C-Reactive Protein in Plasma
    Article Snippet: The mouse antihuman CRP mAb CRP-8 (Sigma-Aldrich, St. Louis, MO, USA; catalog number: C1688; lot number: 025M4863V) was immobilized onto microtiter wells (Thermo Fisher Scientific; catalog number: 468667, 442404; lot number: 148860, 148034; Corning, NY, USA; catalog number: 42592; lot number: 10917007) at 1:1,000 in coating buffer (10-mM sodium carbonate/bicarbonate, pH 9.6) overnight at 4°C. .. Wells were washed with TBS containing 0.02% NP-40, and then blocked with 1% BSA in TBS (blocking buffer).

    Enzyme-linked Immunosorbent Assay:

    Article Title: “Glowing Head” Mice: A Genetic Tool Enabling Reliable Preclinical Image-Based Evaluation of Cancers in Immunocompetent Allografts
    Article Snippet: .. To analyze anti-GFP antibody in serum, ELISA plates (Nunc MaxiSorp, cat# 439454, Thermo Scientific, Waltham, MA, USA) were coated with 31.25 ng of recombinant GFP (MB-0752, Vector Laboratory, Burlingame, CA, USA) in each well overnight at 4°C. .. The next day, sera and control monoclonal anti-GFP antibody (11814460001, Roche Applied Science, Indianapolis, IN, USA) were subjected to serial dilution with blocking solution (3% milk in phosphate-buffered saline [PBS]) to reach the range 1∶25–1∶2000 for the former and 6.25–200 ng/ml for the latter.

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *
    Article Snippet: Paragraph title: ELISA ... For quantification of Aβx-40 and Aβx-42 , 96-well Maxisorp plates (catalog no. 442404, Nunc, Thermo Scientific) were used for ELISAs and coated ON at 4 °C on a shaker with a monoclonal capturing antibody that recognizes Aβ3–8 (1C3, 150 ng/well ( )).

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: Different muscle extracts were normalized over the total amount of proteins, and E2 expression was determined by enzyme-linked immunosorbent assay (ELISA). .. The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6).

    Article Title: Tau Antibody Targeting Pathological Species Blocks Neuronal Uptake and Interneuron Propagation of Tau in Vitro
    Article Snippet: Paragraph title: AT8 ELISA ... Briefly, a 96-well plate (442404; Nunc MaxiSorp) was precoated with 2 μg/mL AT8 antibody (MN1020; 50 μL/well; Thermo Scientific) in PBS overnight at 4°C.

    Article Title: A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1 [W]A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1 [W] [OA]
    Article Snippet: .. Flat-bottom 96-well plates tested for use in the ELISA were Immulon 1B, Immulon 2HB, Immulon 4HB, Nunc 269620, and Nunc 439454 (Thermo Fisher Scientific) and Costar 2507, Costar 3590, and Costar 3598 (Corning Life Sciences). .. Initial experiments were carried out with several polysaccharides over a broad concentration range (1 ng well−1 to 10 μ g well−1 ) in order to determine the maximum loading of polysaccharides onto the plates.

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: The binding of VH domains to Aβ and IAPP was characterized by an ELISA assay conducted in 96-well plates. .. The antigens were diluted to 2.5 μ m , and 100 μl was added to a Nunc-Immuno 96-well plate with MaxiSorp surface coating (442404, Thermo Fisher Scientific) and incubated overnight at room temperature.

    Article Title: Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
    Article Snippet: For measuring the concentration of ICAM-1 by ELISA in cell homogenates (per mg of protein), the MG-63 cells were then harvested by trypsin-EDTA solution in phosphate- buffered saline (Sigma) and RAW 264.7 cells by 0.05% EDTA in phosphate-buffered saline and counted in a Bürker hemocytometer. .. Aliquots of the cell homogenates corresponding to 1–50 μg of protein in 50 μL of water were adsorbed on 96-well microtiter plates (Nunc-Immuno™ Plates, F96 MaxiSorp, Cat. No. 442404, Nunc a/s, Roskilde, Denmark) at 4°C overnight.

    Article Title: Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Article Snippet: Paragraph title: Binding to recombinant cytokines by ELISA ... Plastic 96-well Maxisorp plates (ThermoFisher ref. 442404) were coated with 100 ng/well of recombinant cytokine, washed and then incubated with various concentrations of CALY-002 or control antibodies, tested in duplicate wells.

    Article Title: Ectopic Expression of Snail and Twist in Ph+ Leukemia Cells Upregulates CD44 Expression and Alters Their Differentiation Potential
    Article Snippet: Cellular autophosphorylation of BCR/ABL Cellular autophosphorylation assay was used to monitor levels of phosphorylated BCRABL in treated cells following the ELISA format. .. Using clear flat-bottom immuno nonsterile 96-well plates (Thermo Fisher Scientific), wells were coated with 50 ng/well of mouse monoclonal c-ABL (24-11) and incubated overnight at room temperature (RT).

    Article Title: An ELISA Assay for Quantifying Monomeric C-Reactive Protein in Plasma
    Article Snippet: .. ELISA Assay Quantifying mCRP The mouse antihuman CRP mAb CRP-8 (Sigma-Aldrich, St. Louis, MO, USA; catalog number: C1688; lot number: 025M4863V) was immobilized onto microtiter wells (Thermo Fisher Scientific; catalog number: 468667, 442404; lot number: 148860, 148034; Corning, NY, USA; catalog number: 42592; lot number: 10917007) at 1:1,000 in coating buffer (10-mM sodium carbonate/bicarbonate, pH 9.6) overnight at 4°C. ..

    Article Title: Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model
    Article Snippet: .. Human and mouse CD1d, hCD1dmu and muCD1dhu were coated on 96-well NuncTM MaxisorpTM ELISA Plates (ThermoFisher, 442404) at 1 μg/mL in PBS overnight at 4°C. ..

    Article Title: An ELISA Assay for Quantifying Monomeric C-Reactive Protein in Plasma
    Article Snippet: Paragraph title: ELISA Assay Quantifying mCRP ... The mouse antihuman CRP mAb CRP-8 (Sigma-Aldrich, St. Louis, MO, USA; catalog number: C1688; lot number: 025M4863V) was immobilized onto microtiter wells (Thermo Fisher Scientific; catalog number: 468667, 442404; lot number: 148860, 148034; Corning, NY, USA; catalog number: 42592; lot number: 10917007) at 1:1,000 in coating buffer (10-mM sodium carbonate/bicarbonate, pH 9.6) overnight at 4°C.

    Article Title: A general protocol for the generation of Nanobodies for structural biology
    Article Snippet: .. 245 × 245 × 25mm square dishes (Thermo Scientific, cat. no. 240835) 96-deepwell plates (Thermo Scientific, cat. no. AB-0932) 96-well conical bottom plates (Thermo Scientific, cat. no. 249944) 96-well U-bottom tissue culture plates with lid (BD, cat. no. 353077) Benchtop centrifuge for Falcon tubes and 96-well plates, swing out rotor (Eppendorf, cat. no. 5810R) Blood collection tubes: Venosafe® hematology (Terumo, cat. No. VF-109SDK) and serum gel (Terumo, cat. No. VF-108SAS) tubes Cell scraper (Costar, cat. no 3011) Centrifuge for 1.5 and 2 ml tubes (Eppendorf, cat. no. 5430R) Electroporator: E.coli pulser (Bio-rad, cat. no.1652103) Falcon conical centrifuge tubes (15 ml, 50 ml) (Fisher Scientific, cat. no. 14-959-49B, 14-432-22) Gene pulser electroporation cuvette 0.1 cm gap (Bio-rad, cat. no. 165-2089) Glass beads Ø (2.5-3.5 mm): (VWR, cat. no.2010087) Heidolph Titramax 1000 vibrating platform (Heidolph-instruments, cat. no. 544-12200-00) Incubators at 4 °C, 16 °C and 37 °C Incubators with orbital shaking platform at 37 °C, 170 -200 rpm Maxisorp 96-well Immuno plates (Nunc, cat. no. 439454) Multichannel pipettes: Finnpipette F2 8 Channels 30-300 μl (Thermo Scientific, cat. no. 4662030), Finnpipette F2 8 Channels 5-50 μl (Thermo Scientific, cat. no. 4662010) Nunc Microplate Sealing Tape 236366 (Thermo Scientific, cat. no. 10170671) PCR tube strips (8-tube strips, Greiner bio-one, cat. no.673210; cap strips, Greiner bio-one, cat. no.373270) RNase free microtubes (Eppendorf, cat no. 0030 121.589) Shake flask triple baffled (1 liter, 250 ml) (Sigma-Aldrich, cat. no. F0769, F0644) Spectrophotometer & ELISA plate reader Sterile gas permeable adhesive seal (Thermo Scientific, cat. no. AB-0718) Sterilization filters: 0.22 μM and 0.45 μM (Sarstedt , cat. no.83-1826-001, cat. no.83-1826) Uni-SepMAXI+ lymphocyte separation tubes (Gentaur, cat. No. NVU16) ..

    Sandwich ELISA:

    Article Title: A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma
    Article Snippet: 4.2 Enzyme linked immunosorbent assays CIZ1b-fibrinogen alpha chain complex was detected by sandwich ELISA after partial denaturation of 5 μl of plasma in 100 μl of 0.5% tween20, 1% SDS in PBS, supplemented with 0.5 mM PMSF and incubation at 20 °C for 30 min with repeated vortexing. .. Plates (Nunc Maxisorp 442404) were coated with 1 μg of 043 CIZ1b pAb protein A fraction (Pierce 20356), overnight at 4 °C in 100 μl of 50 mM carbonate pH 9.6, then blocked for 2 h at room temperature with 300 μl of filtered 1% BSA (Jackson ImmunoResearch 1000162) in PBS.

    Incubation:

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *
    Article Snippet: For quantification of Aβx-40 and Aβx-42 , 96-well Maxisorp plates (catalog no. 442404, Nunc, Thermo Scientific) were used for ELISAs and coated ON at 4 °C on a shaker with a monoclonal capturing antibody that recognizes Aβ3–8 (1C3, 150 ng/well ( )). .. Biotinylated polyclonal rabbit anti-Aβx-42 or anti-Aβx-40 detection antibody was incubated at a final concentration of 0.32 μg/ml or 0.66 μg/ml, respectively, for 1 h at room temperature on shaker before being detected with a 1:2000 dilution of streptavidin HRP (catalog no. 3310-9, Mabtech) for 30 min at room temperature on a shaker.

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6). .. After overnight incubation, the plates were washed (1× PBS, 0.05% Tween 20) and incubated for 1 h at 37°C with 250 μl of blocking buffer (2% bovine serum albumin [BSA], 1× PBS, 0.05% Tween 20) per well.

    Article Title: Tau Antibody Targeting Pathological Species Blocks Neuronal Uptake and Interneuron Propagation of Tau in Vitro
    Article Snippet: Briefly, a 96-well plate (442404; Nunc MaxiSorp) was precoated with 2 μg/mL AT8 antibody (MN1020; 50 μL/well; Thermo Scientific) in PBS overnight at 4°C. .. After washing with PBS-T three times, standards and samples were loaded onto the plate (50 μL/well) and incubated overnight at 4°C.

    Article Title: A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma
    Article Snippet: 4.2 Enzyme linked immunosorbent assays CIZ1b-fibrinogen alpha chain complex was detected by sandwich ELISA after partial denaturation of 5 μl of plasma in 100 μl of 0.5% tween20, 1% SDS in PBS, supplemented with 0.5 mM PMSF and incubation at 20 °C for 30 min with repeated vortexing. .. Plates (Nunc Maxisorp 442404) were coated with 1 μg of 043 CIZ1b pAb protein A fraction (Pierce 20356), overnight at 4 °C in 100 μl of 50 mM carbonate pH 9.6, then blocked for 2 h at room temperature with 300 μl of filtered 1% BSA (Jackson ImmunoResearch 1000162) in PBS.

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: .. The antigens were diluted to 2.5 μ m , and 100 μl was added to a Nunc-Immuno 96-well plate with MaxiSorp surface coating (442404, Thermo Fisher Scientific) and incubated overnight at room temperature. ..

    Article Title: Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Article Snippet: .. Plastic 96-well Maxisorp plates (ThermoFisher ref. 442404) were coated with 100 ng/well of recombinant cytokine, washed and then incubated with various concentrations of CALY-002 or control antibodies, tested in duplicate wells. .. Recombinant cytokines used were: human IL-15 produced in E. Coli (Prospec, ref. CYT-230), rhesus/cynomolgus monkey IL-15 produced in yeast (myBioSource ref MBS948894), rat IL-15 produced in E. Coli (Sigma ref. SRP-4172), mouse IL-15 produced in E. Coli (R & D Systems ref. 447-ML), and human IL-2 produced in E. Coli (R & D Systems ref. 202-IL).

    Article Title: Ectopic Expression of Snail and Twist in Ph+ Leukemia Cells Upregulates CD44 Expression and Alters Their Differentiation Potential
    Article Snippet: .. Using clear flat-bottom immuno nonsterile 96-well plates (Thermo Fisher Scientific), wells were coated with 50 ng/well of mouse monoclonal c-ABL (24-11) and incubated overnight at room temperature (RT). .. The next day, wells were washed five times with PBS + 0.05% (w/v) Tween 20 and blocking buffer (3% w/v bovine serum albumin [BSA] in PBS) was added for 2 h at RT.

    Expressing:

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: Paragraph title: E2 protein expression in mouse muscle. ... The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6).

    Electroporation:

    Article Title: A general protocol for the generation of Nanobodies for structural biology
    Article Snippet: .. 245 × 245 × 25mm square dishes (Thermo Scientific, cat. no. 240835) 96-deepwell plates (Thermo Scientific, cat. no. AB-0932) 96-well conical bottom plates (Thermo Scientific, cat. no. 249944) 96-well U-bottom tissue culture plates with lid (BD, cat. no. 353077) Benchtop centrifuge for Falcon tubes and 96-well plates, swing out rotor (Eppendorf, cat. no. 5810R) Blood collection tubes: Venosafe® hematology (Terumo, cat. No. VF-109SDK) and serum gel (Terumo, cat. No. VF-108SAS) tubes Cell scraper (Costar, cat. no 3011) Centrifuge for 1.5 and 2 ml tubes (Eppendorf, cat. no. 5430R) Electroporator: E.coli pulser (Bio-rad, cat. no.1652103) Falcon conical centrifuge tubes (15 ml, 50 ml) (Fisher Scientific, cat. no. 14-959-49B, 14-432-22) Gene pulser electroporation cuvette 0.1 cm gap (Bio-rad, cat. no. 165-2089) Glass beads Ø (2.5-3.5 mm): (VWR, cat. no.2010087) Heidolph Titramax 1000 vibrating platform (Heidolph-instruments, cat. no. 544-12200-00) Incubators at 4 °C, 16 °C and 37 °C Incubators with orbital shaking platform at 37 °C, 170 -200 rpm Maxisorp 96-well Immuno plates (Nunc, cat. no. 439454) Multichannel pipettes: Finnpipette F2 8 Channels 30-300 μl (Thermo Scientific, cat. no. 4662030), Finnpipette F2 8 Channels 5-50 μl (Thermo Scientific, cat. no. 4662010) Nunc Microplate Sealing Tape 236366 (Thermo Scientific, cat. no. 10170671) PCR tube strips (8-tube strips, Greiner bio-one, cat. no.673210; cap strips, Greiner bio-one, cat. no.373270) RNase free microtubes (Eppendorf, cat no. 0030 121.589) Shake flask triple baffled (1 liter, 250 ml) (Sigma-Aldrich, cat. no. F0769, F0644) Spectrophotometer & ELISA plate reader Sterile gas permeable adhesive seal (Thermo Scientific, cat. no. AB-0718) Sterilization filters: 0.22 μM and 0.45 μM (Sarstedt , cat. no.83-1826-001, cat. no.83-1826) Uni-SepMAXI+ lymphocyte separation tubes (Gentaur, cat. No. NVU16) ..

    Activation Assay:

    Article Title: Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
    Article Snippet: Paragraph title: Potential immune activation of cells on nanofibrous membranes ... Aliquots of the cell homogenates corresponding to 1–50 μg of protein in 50 μL of water were adsorbed on 96-well microtiter plates (Nunc-Immuno™ Plates, F96 MaxiSorp, Cat. No. 442404, Nunc a/s, Roskilde, Denmark) at 4°C overnight.

    Serial Dilution:

    Article Title: “Glowing Head” Mice: A Genetic Tool Enabling Reliable Preclinical Image-Based Evaluation of Cancers in Immunocompetent Allografts
    Article Snippet: To analyze anti-GFP antibody in serum, ELISA plates (Nunc MaxiSorp, cat# 439454, Thermo Scientific, Waltham, MA, USA) were coated with 31.25 ng of recombinant GFP (MB-0752, Vector Laboratory, Burlingame, CA, USA) in each well overnight at 4°C. .. The next day, sera and control monoclonal anti-GFP antibody (11814460001, Roche Applied Science, Indianapolis, IN, USA) were subjected to serial dilution with blocking solution (3% milk in phosphate-buffered saline [PBS]) to reach the range 1∶25–1∶2000 for the former and 6.25–200 ng/ml for the latter.

    Article Title: A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma
    Article Snippet: Plates (Nunc Maxisorp 442404) were coated with 1 μg of 043 CIZ1b pAb protein A fraction (Pierce 20356), overnight at 4 °C in 100 μl of 50 mM carbonate pH 9.6, then blocked for 2 h at room temperature with 300 μl of filtered 1% BSA (Jackson ImmunoResearch 1000162) in PBS. .. For measurement of fibrinogen capture antibody was chicken IgY (1 μg/well, Genway 15-288-22856), and analyte concentration was reduced five orders of magnitude by serial dilution.

    Cell Culture:

    Article Title: Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
    Article Snippet: The concentrations of TNF-α in cell culture media were determined from calibration curves and given in pg per 100,000 cells, ie, not per mL of the culture media, as usual., The reason was that the cell numbers on the tested materials were different. .. Aliquots of the cell homogenates corresponding to 1–50 μg of protein in 50 μL of water were adsorbed on 96-well microtiter plates (Nunc-Immuno™ Plates, F96 MaxiSorp, Cat. No. 442404, Nunc a/s, Roskilde, Denmark) at 4°C overnight.

    Polymerase Chain Reaction:

    Article Title: A general protocol for the generation of Nanobodies for structural biology
    Article Snippet: .. 245 × 245 × 25mm square dishes (Thermo Scientific, cat. no. 240835) 96-deepwell plates (Thermo Scientific, cat. no. AB-0932) 96-well conical bottom plates (Thermo Scientific, cat. no. 249944) 96-well U-bottom tissue culture plates with lid (BD, cat. no. 353077) Benchtop centrifuge for Falcon tubes and 96-well plates, swing out rotor (Eppendorf, cat. no. 5810R) Blood collection tubes: Venosafe® hematology (Terumo, cat. No. VF-109SDK) and serum gel (Terumo, cat. No. VF-108SAS) tubes Cell scraper (Costar, cat. no 3011) Centrifuge for 1.5 and 2 ml tubes (Eppendorf, cat. no. 5430R) Electroporator: E.coli pulser (Bio-rad, cat. no.1652103) Falcon conical centrifuge tubes (15 ml, 50 ml) (Fisher Scientific, cat. no. 14-959-49B, 14-432-22) Gene pulser electroporation cuvette 0.1 cm gap (Bio-rad, cat. no. 165-2089) Glass beads Ø (2.5-3.5 mm): (VWR, cat. no.2010087) Heidolph Titramax 1000 vibrating platform (Heidolph-instruments, cat. no. 544-12200-00) Incubators at 4 °C, 16 °C and 37 °C Incubators with orbital shaking platform at 37 °C, 170 -200 rpm Maxisorp 96-well Immuno plates (Nunc, cat. no. 439454) Multichannel pipettes: Finnpipette F2 8 Channels 30-300 μl (Thermo Scientific, cat. no. 4662030), Finnpipette F2 8 Channels 5-50 μl (Thermo Scientific, cat. no. 4662010) Nunc Microplate Sealing Tape 236366 (Thermo Scientific, cat. no. 10170671) PCR tube strips (8-tube strips, Greiner bio-one, cat. no.673210; cap strips, Greiner bio-one, cat. no.373270) RNase free microtubes (Eppendorf, cat no. 0030 121.589) Shake flask triple baffled (1 liter, 250 ml) (Sigma-Aldrich, cat. no. F0769, F0644) Spectrophotometer & ELISA plate reader Sterile gas permeable adhesive seal (Thermo Scientific, cat. no. AB-0718) Sterilization filters: 0.22 μM and 0.45 μM (Sarstedt , cat. no.83-1826-001, cat. no.83-1826) Uni-SepMAXI+ lymphocyte separation tubes (Gentaur, cat. No. NVU16) ..

    Sonication:

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: The IAPP fibrils were sonicated on ice for 10 s at 75% power (3 times) with 30 s of cooling between rounds of sonication. .. The antigens were diluted to 2.5 μ m , and 100 μl was added to a Nunc-Immuno 96-well plate with MaxiSorp surface coating (442404, Thermo Fisher Scientific) and incubated overnight at room temperature.

    Injection:

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: At different times after injection, animals were sacrificed and quadriceps muscles were removed and kept in liquid nitrogen. .. The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6).

    Recombinant:

    Article Title: “Glowing Head” Mice: A Genetic Tool Enabling Reliable Preclinical Image-Based Evaluation of Cancers in Immunocompetent Allografts
    Article Snippet: .. To analyze anti-GFP antibody in serum, ELISA plates (Nunc MaxiSorp, cat# 439454, Thermo Scientific, Waltham, MA, USA) were coated with 31.25 ng of recombinant GFP (MB-0752, Vector Laboratory, Burlingame, CA, USA) in each well overnight at 4°C. .. The next day, sera and control monoclonal anti-GFP antibody (11814460001, Roche Applied Science, Indianapolis, IN, USA) were subjected to serial dilution with blocking solution (3% milk in phosphate-buffered saline [PBS]) to reach the range 1∶25–1∶2000 for the former and 6.25–200 ng/ml for the latter.

    Article Title: Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Article Snippet: .. Plastic 96-well Maxisorp plates (ThermoFisher ref. 442404) were coated with 100 ng/well of recombinant cytokine, washed and then incubated with various concentrations of CALY-002 or control antibodies, tested in duplicate wells. .. Recombinant cytokines used were: human IL-15 produced in E. Coli (Prospec, ref. CYT-230), rhesus/cynomolgus monkey IL-15 produced in yeast (myBioSource ref MBS948894), rat IL-15 produced in E. Coli (Sigma ref. SRP-4172), mouse IL-15 produced in E. Coli (R & D Systems ref. 447-ML), and human IL-2 produced in E. Coli (R & D Systems ref. 202-IL).

    Labeling:

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: The antigens were diluted to 2.5 μ m , and 100 μl was added to a Nunc-Immuno 96-well plate with MaxiSorp surface coating (442404, Thermo Fisher Scientific) and incubated overnight at room temperature. .. VH domains that were labeled with a biotin tag (EZ-Link Sulfo-NHS-LC-Biotin, 21335, Thermo Fisher Scientific) were diluted to varying concentrations.

    Titration:

    Article Title: Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model
    Article Snippet: Human and mouse CD1d, hCD1dmu and muCD1dhu were coated on 96-well NuncTM MaxisorpTM ELISA Plates (ThermoFisher, 442404) at 1 μg/mL in PBS overnight at 4°C. .. NIB.2 or controls in diluent (PBS + 1% BSA + 0.05% Tween-20) were added to the wells in a half log titration starting from 10 μg/mL, and no antibody (0 μg/mL) was included as a negative control.

    Construct:

    Article Title: Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model
    Article Snippet: Paragraph title: CD1d human:mouse construct ELISA ... Human and mouse CD1d, hCD1dmu and muCD1dhu were coated on 96-well NuncTM MaxisorpTM ELISA Plates (ThermoFisher, 442404) at 1 μg/mL in PBS overnight at 4°C.

    Mouse Assay:

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *
    Article Snippet: For quantification of Aβx-40 and Aβx-42 , 96-well Maxisorp plates (catalog no. 442404, Nunc, Thermo Scientific) were used for ELISAs and coated ON at 4 °C on a shaker with a monoclonal capturing antibody that recognizes Aβ3–8 (1C3, 150 ng/well ( )). .. Tween and SDS extracts from brains from 2- to 3-month-old mice were mixed 1:1 and diluted 1:30 in 0.1% BSA for the Aβx-40 ELISA and 1:40 in 0.1% BSA for the Aβx-42 ELISA.

    Negative Control:

    Article Title: Potent neutralizing anti-CD1d antibody reduces lung cytokine release in primate asthma model
    Article Snippet: Human and mouse CD1d, hCD1dmu and muCD1dhu were coated on 96-well NuncTM MaxisorpTM ELISA Plates (ThermoFisher, 442404) at 1 μg/mL in PBS overnight at 4°C. .. NIB.2 or controls in diluent (PBS + 1% BSA + 0.05% Tween-20) were added to the wells in a half log titration starting from 10 μg/mL, and no antibody (0 μg/mL) was included as a negative control.

    Binding Assay:

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: Paragraph title: Antibody Binding Analysis ... The antigens were diluted to 2.5 μ m , and 100 μl was added to a Nunc-Immuno 96-well plate with MaxiSorp surface coating (442404, Thermo Fisher Scientific) and incubated overnight at room temperature.

    Article Title: Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
    Article Snippet: Aliquots of the cell homogenates corresponding to 1–50 μg of protein in 50 μL of water were adsorbed on 96-well microtiter plates (Nunc-Immuno™ Plates, F96 MaxiSorp, Cat. No. 442404, Nunc a/s, Roskilde, Denmark) at 4°C overnight. .. After washing twice with phosphate-buffered saline (100 μL/well), the nonspecific binding sites were blocked by 0.02% gelatin in phosphate-buffered saline (100 μL/well, 60 minutes).

    Article Title: Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Article Snippet: Paragraph title: Binding to recombinant cytokines by ELISA ... Plastic 96-well Maxisorp plates (ThermoFisher ref. 442404) were coated with 100 ng/well of recombinant cytokine, washed and then incubated with various concentrations of CALY-002 or control antibodies, tested in duplicate wells.

    Homogenization:

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: Extracts were prepared by homogenization (Polytron; KinematicaAG Littau-Luzern) of muscles in 1 ml of lysis buffer (catalog number 9581902; Promega Corporation, Madison, Wis.) and three cycles of freeze-thawing. .. The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6).

    Spectrophotometry:

    Article Title: A general protocol for the generation of Nanobodies for structural biology
    Article Snippet: .. 245 × 245 × 25mm square dishes (Thermo Scientific, cat. no. 240835) 96-deepwell plates (Thermo Scientific, cat. no. AB-0932) 96-well conical bottom plates (Thermo Scientific, cat. no. 249944) 96-well U-bottom tissue culture plates with lid (BD, cat. no. 353077) Benchtop centrifuge for Falcon tubes and 96-well plates, swing out rotor (Eppendorf, cat. no. 5810R) Blood collection tubes: Venosafe® hematology (Terumo, cat. No. VF-109SDK) and serum gel (Terumo, cat. No. VF-108SAS) tubes Cell scraper (Costar, cat. no 3011) Centrifuge for 1.5 and 2 ml tubes (Eppendorf, cat. no. 5430R) Electroporator: E.coli pulser (Bio-rad, cat. no.1652103) Falcon conical centrifuge tubes (15 ml, 50 ml) (Fisher Scientific, cat. no. 14-959-49B, 14-432-22) Gene pulser electroporation cuvette 0.1 cm gap (Bio-rad, cat. no. 165-2089) Glass beads Ø (2.5-3.5 mm): (VWR, cat. no.2010087) Heidolph Titramax 1000 vibrating platform (Heidolph-instruments, cat. no. 544-12200-00) Incubators at 4 °C, 16 °C and 37 °C Incubators with orbital shaking platform at 37 °C, 170 -200 rpm Maxisorp 96-well Immuno plates (Nunc, cat. no. 439454) Multichannel pipettes: Finnpipette F2 8 Channels 30-300 μl (Thermo Scientific, cat. no. 4662030), Finnpipette F2 8 Channels 5-50 μl (Thermo Scientific, cat. no. 4662010) Nunc Microplate Sealing Tape 236366 (Thermo Scientific, cat. no. 10170671) PCR tube strips (8-tube strips, Greiner bio-one, cat. no.673210; cap strips, Greiner bio-one, cat. no.373270) RNase free microtubes (Eppendorf, cat no. 0030 121.589) Shake flask triple baffled (1 liter, 250 ml) (Sigma-Aldrich, cat. no. F0769, F0644) Spectrophotometer & ELISA plate reader Sterile gas permeable adhesive seal (Thermo Scientific, cat. no. AB-0718) Sterilization filters: 0.22 μM and 0.45 μM (Sarstedt , cat. no.83-1826-001, cat. no.83-1826) Uni-SepMAXI+ lymphocyte separation tubes (Gentaur, cat. No. NVU16) ..

    Evaporation:

    Article Title: A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1 [W]A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1 [W] [OA]
    Article Snippet: Flat-bottom 96-well plates tested for use in the ELISA were Immulon 1B, Immulon 2HB, Immulon 4HB, Nunc 269620, and Nunc 439454 (Thermo Fisher Scientific) and Costar 2507, Costar 3590, and Costar 3598 (Corning Life Sciences). .. Polysaccharides were applied (50 μ L of 10 μ g mL−1 in deionized water per well) to 96-well plates and were dried to the well surfaces by evaporation overnight at 37°C.

    Produced:

    Article Title: Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Article Snippet: Plastic 96-well Maxisorp plates (ThermoFisher ref. 442404) were coated with 100 ng/well of recombinant cytokine, washed and then incubated with various concentrations of CALY-002 or control antibodies, tested in duplicate wells. .. Recombinant cytokines used were: human IL-15 produced in E. Coli (Prospec, ref. CYT-230), rhesus/cynomolgus monkey IL-15 produced in yeast (myBioSource ref MBS948894), rat IL-15 produced in E. Coli (Sigma ref. SRP-4172), mouse IL-15 produced in E. Coli (R & D Systems ref. 447-ML), and human IL-2 produced in E. Coli (R & D Systems ref. 202-IL).

    Concentration Assay:

    Article Title: Overexpression of Heparanase Lowers the Amyloid Burden in Amyloid-β Precursor Protein Transgenic Mice *
    Article Snippet: For quantification of Aβx-40 and Aβx-42 , 96-well Maxisorp plates (catalog no. 442404, Nunc, Thermo Scientific) were used for ELISAs and coated ON at 4 °C on a shaker with a monoclonal capturing antibody that recognizes Aβ3–8 (1C3, 150 ng/well ( )). .. Biotinylated polyclonal rabbit anti-Aβx-42 or anti-Aβx-40 detection antibody was incubated at a final concentration of 0.32 μg/ml or 0.66 μg/ml, respectively, for 1 h at room temperature on shaker before being detected with a 1:2000 dilution of streptavidin HRP (catalog no. 3310-9, Mabtech) for 30 min at room temperature on a shaker.

    Article Title: A quantitative immunoassay for lung cancer biomarker CIZ1b in patient plasma
    Article Snippet: Plates (Nunc Maxisorp 442404) were coated with 1 μg of 043 CIZ1b pAb protein A fraction (Pierce 20356), overnight at 4 °C in 100 μl of 50 mM carbonate pH 9.6, then blocked for 2 h at room temperature with 300 μl of filtered 1% BSA (Jackson ImmunoResearch 1000162) in PBS. .. For measurement of fibrinogen capture antibody was chicken IgY (1 μg/well, Genway 15-288-22856), and analyte concentration was reduced five orders of magnitude by serial dilution.

    Article Title: A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1 [W]A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies 1 [W] [OA]
    Article Snippet: Flat-bottom 96-well plates tested for use in the ELISA were Immulon 1B, Immulon 2HB, Immulon 4HB, Nunc 269620, and Nunc 439454 (Thermo Fisher Scientific) and Costar 2507, Costar 3590, and Costar 3598 (Corning Life Sciences). .. Initial experiments were carried out with several polysaccharides over a broad concentration range (1 ng well−1 to 10 μ g well−1 ) in order to determine the maximum loading of polysaccharides onto the plates.

    Article Title: Design and Optimization of Anti-amyloid Domain Antibodies Specific for β-Amyloid and Islet Amyloid Polypeptide *
    Article Snippet: After lyophilization, the dried peptide was dissolved in 20 m m Tris (pH 7.4) to achieve a final concentration of 32 μ m . .. The antigens were diluted to 2.5 μ m , and 100 μl was added to a Nunc-Immuno 96-well plate with MaxiSorp surface coating (442404, Thermo Fisher Scientific) and incubated overnight at room temperature.

    Article Title: Nanofibrous poly(lactide-co-glycolide) membranes loaded with diamond nanoparticles as promising substrates for bone tissue engineering
    Article Snippet: For measuring the concentration of ICAM-1 by ELISA in cell homogenates (per mg of protein), the MG-63 cells were then harvested by trypsin-EDTA solution in phosphate- buffered saline (Sigma) and RAW 264.7 cells by 0.05% EDTA in phosphate-buffered saline and counted in a Bürker hemocytometer. .. Aliquots of the cell homogenates corresponding to 1–50 μg of protein in 50 μL of water were adsorbed on 96-well microtiter plates (Nunc-Immuno™ Plates, F96 MaxiSorp, Cat. No. 442404, Nunc a/s, Roskilde, Denmark) at 4°C overnight.

    Marker:

    Article Title: “Glowing Head” Mice: A Genetic Tool Enabling Reliable Preclinical Image-Based Evaluation of Cancers in Immunocompetent Allografts
    Article Snippet: Paragraph title: Hormone and immunological marker analysis ... To analyze anti-GFP antibody in serum, ELISA plates (Nunc MaxiSorp, cat# 439454, Thermo Scientific, Waltham, MA, USA) were coated with 31.25 ng of recombinant GFP (MB-0752, Vector Laboratory, Burlingame, CA, USA) in each well overnight at 4°C.

    Lysis:

    Article Title: Enhancing B- and T-Cell Immune Response to a Hepatitis C Virus E2 DNA Vaccine by Intramuscular Electrical Gene Transfer
    Article Snippet: Extracts were prepared by homogenization (Polytron; KinematicaAG Littau-Luzern) of muscles in 1 ml of lysis buffer (catalog number 9581902; Promega Corporation, Madison, Wis.) and three cycles of freeze-thawing. .. The 96-well plates (Nunc-Immuno Plate MaxiSorp Surface, catalog number 439454; Nunc A/S, Roskilde, Denmark) were coated with 1 μg of Galanthus Nivalis Lectin (GNA; catalog number L-8275; Sigma Chemical Co., St. Louis, Mo.) per well in coating buffer (50 mM NaHCO3 , pH 9.6).

    Article Title: Ectopic Expression of Snail and Twist in Ph+ Leukemia Cells Upregulates CD44 Expression and Alters Their Differentiation Potential
    Article Snippet: Cells were collected, washed twice with cold PBS, and lysed in 1X cell lysis buffer (Cell Signaling Technologies). .. Using clear flat-bottom immuno nonsterile 96-well plates (Thermo Fisher Scientific), wells were coated with 50 ng/well of mouse monoclonal c-ABL (24-11) and incubated overnight at room temperature (RT).

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    Clear Flat Bottom Immuno Nonsterile 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher laminin
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