ba1 insensitive j k skeeles f241 95 931 clinical  (ATCC)

Journal:

Article Title: Discovery, Purification, and Characterization of a Temperate Transducing Bacteriophage for Bordetella avium

doi:

Figure Lengend Snippet: Bacterial and bacteriophage strains

Article Snippet: Phage sensitivity spot tests with these two strains employed a minimal medium (Stainer-Scholte) described by Hewlett and Wolff ( 16 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain Description Source or reference B. avium 197N Parental strain; relevant phenotype: Ba1 s Ba2 s Nal r Str s Lac − Kan s Mot + Hag + 37 197N2 Spontaneous streptomycin-resistant mutant of 197N This study G145 197N except hag ::mini-Tn 5lacZ2 Hag − Kan r Lac + This study G146 197N except mot ::mini-Tn 5lacZ2 Mot − Kan r Lac + 37 1B1 197N with random insertion (Ω1) using mini-Tn 5 This study 1B4 197N with random insertion (Ω2) using mini-Tn 5 This study 1B5 197N with random insertion (Ω3) using mini-Tn 5 This study AP21 197N lysogenized by Ba1 This study GOBL271 a Strain screened for temperate phage (Ba1 and Ba2, sensitive) 15 35086 Strain screened for temperate phage (lysogenic for Ba2; resistant to Ba1 and Ba2) ATCC b Wampler Strain screened for temperate phage (lysogenic for Ba1; Ba2 sensitive) 22 F247-P4164 Clinical isolate; Ba1 sensitive J. K. Skeeles F242-95-950 Clinical isolate; Ba1 insensitive J. K. Skeeles F241-95-931 Clinical isolate; Ba1 sensitive J. K. Skeeles F243-P4485 Clinical isolate; Ba1 sensitive J. K. Skeeles TR96-1212 Clinical isolate; Ba1 insensitive M. Blakeley Ba169 Clinical isolate; Ba1 sensitive 19 Ba198 Clinical isolate; Ba1 sensitive 19 Ba177 Clinical isolate; Ba1 insensitive; lysogenic 19 Ba002 Clinical isolate; Ba1 sensitive 19 B. hinzii Ba008 Clinical isolate; Ba1 insensitive Y. M. Saif B. bronchiseptica 17640 Clinical isolate; Ba1 insensitive 23 110NH Clinical isolate; Ba1 insensitive 4 110H Clinical isolate; Ba1 insensitive 4 R-5 Clinical isolate; Ba1 insensitive 6 Romark Clinical isolate; Ba1 insensitive 24 87 Clinical isolate; Ba1 insensitive 4 64-C-0406 Clinical isolate; Ba1 insensitive Laboratory collection JS34682 Clinical isolate; Ba1 insensitive 24 BB213 Clinical isolate; Ba1 insensitive E. Hewlett BB361 Clinical isolate; Ba1 insensitive 24 B. pertussis 388 Ba1 insensitive 40 B. parapertussis 253 Ba1 insensitive 25 Bacteriophage Ba1 Wild type; isolated from B. avium strain Wampler This study Ba1c1 Clear-plaque mutant of Ba1 This study Ba2 Temperate B. avium phage isolated from ATCC strain 35086 This study Open in a separate window a GOBL271 is derived from the same lineage as 197N (i.e., the parental strain of both is 197 [ 14 ]).

Techniques: Mutagenesis, Isolation

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Effects of CAPRIN2 on the ferroptosis and survival of ECM-detached NPC cells and the migration and invasion of NPC cells. (A) Viability assay of ECM-detached NPC cell lines treated with erastin (5 μM) and/or ferrostatin-1 (1 μM) for 24 h (B) Viability assay of the indicated stable 5-8F or C666-1 cell lines cultured under ECM detachment conditions for 72 h For (A, B) , the experiments were repeated three times, and the data are shown as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. (C, D) Migration and invasion assays of the indicated stable 5-8F (C) and C666-1 (D) cell lines. These assays were conducted in triplicate. Representative images are displayed. The data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Migration, Viability Assay, Cell Culture

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: CAPRIN2 promotes the ferroptosis resistance, survival, migration and invasion of NPC cells through HMGCR. (A) Overexpression of HMGCR partially reverses the effects of CAPRIN2 on the ferroptosis of ECM-detached 5-8F (left panel) and C666-1 (right panel) cells. The NPC cell lines were treated with erastin (5 μM) for 24 h (B, C) MDA assay (B) and GSH assay (C) results of erastin-treated NPC stable cell lines as indicated. (D) Ectopic expression of HMGCR partially rescues the effects of CAPRIN2 knockdown on ECM-detached NPC cell survival. For (A–C) and (D) , the experiments were conducted in triplicate, and the data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. (E, F) Stable overexpression of HMGCR partially reverses the effects of CAPRIN2 knockdown on 5-8F (E) and C666-1 (F) cell migration and invasion. Representative images of three independent experiments are shown. The data are expressed as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Migration, Over Expression, Multiple Displacement Amplification, GSH Assay, Stable Transfection, Expressing

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Regulation of lung colonization capacity via the CAPRIN2/HMGCR axis in NPC cells. (A, B) The inhibitory effect of erastin on the lung metastasis of NPC cells was enhanced by knockdown of the CAPRIN2/HMGCR axis. (C, D) CAPRIN2 promotes the lung colonization of NPC cells through HMGCR. For (A, C) , representative images of lungs and HE staining are shown. The location of lung metastatic nodules is indicated by the arrow. For (B, D) , the number of lung metastases (left panel) and the weight of the lungs (right panel) are given. * p < 0.05, ** p < 0.01.

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Staining

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: LINC00941 acts as an upstream molecule to regulate the biological functions of CAPRIN2. (A) LINC00941 downregulation promoted the ferroptosis of ECM-detached NPC cells, which was partially rescued by CAPRIN2 overexpression. The NPC cells were treated with erastin (5 μM) for 24 h (B) Knockdown of LINC00941 decreased the survival of ECM-detached NPC cells, which could be partially reversed by CAPRIN2 overexpression. For (A) and (B) , the assays were conducted in triplicate, and the data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. (C, D) LINC00941 knockdown inhibited the migration and invasion of 5-8F (C) and C666-1 (D) cells, and overexpression of CAPRIN2 partially reversed this effect. Representative images are shown. The data are provided as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Over Expression, Migration

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: CAPRIN2 is overactivated in NPC tissues and is associated with a poor prognosis in patients. (A) The expression level of CAPRIN2 in 20 NPC tissues and 5 nasopharyngeal tissues. (B) The correlation between LINC00941/CAPRIN2, CAPRIN2/HMGCR or LINC00941/HMGCR in 20 NPC tissues. For (A) and (B) , the expression levels of CAPRIN2, HMGCR and LINC00941 were determined by qRT-PCR. The levels were normalized to those of β-actin and shown as the mean ± SEM. * p < 0.05. (C) Representative immunohistochemical images of normal nasopharyngeal tissues (left panel) and NPC tissues (middle panel). The boxes represent the magnified region. The representative image of negative stained control (right panel, top) shows the negative staining result of NPC tissues incubated with antibody-free serum. The representative image of positive stained control (right panel, bottom) shows the positive staining result of CAPRIN2 in NPC tissues incubated with the primary antibody of CAPRIN2. (D) Kaplan-Meier survival analysis of the association between CAPRIN2 expression and the PFS or OS of NPC patients ( log-rank test ).

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Staining, Negative Staining, Incubation

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Correlations between CAPRIN2 expression and clinicopathological characteristics.

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Univariate and multivariate analysis for OS.

Article Snippet: The siRNAs applied in the study were all products of Santa Cruz (Shanghai, China) and are listed as follows: CAPRIN2 siRNA, HMGCR siRNA and negative control siRNA.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Effects of CAPRIN2 on the ferroptosis and survival of ECM-detached NPC cells and the migration and invasion of NPC cells. (A) Viability assay of ECM-detached NPC cell lines treated with erastin (5 μM) and/or ferrostatin-1 (1 μM) for 24 h (B) Viability assay of the indicated stable 5-8F or C666-1 cell lines cultured under ECM detachment conditions for 72 h For (A, B) , the experiments were repeated three times, and the data are shown as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. (C, D) Migration and invasion assays of the indicated stable 5-8F (C) and C666-1 (D) cell lines. These assays were conducted in triplicate. Representative images are displayed. The data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Migration, Viability Assay, Cell Culture

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: CAPRIN2 promotes the ferroptosis resistance, survival, migration and invasion of NPC cells through HMGCR. (A) Overexpression of HMGCR partially reverses the effects of CAPRIN2 on the ferroptosis of ECM-detached 5-8F (left panel) and C666-1 (right panel) cells. The NPC cell lines were treated with erastin (5 μM) for 24 h (B, C) MDA assay (B) and GSH assay (C) results of erastin-treated NPC stable cell lines as indicated. (D) Ectopic expression of HMGCR partially rescues the effects of CAPRIN2 knockdown on ECM-detached NPC cell survival. For (A–C) and (D) , the experiments were conducted in triplicate, and the data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. (E, F) Stable overexpression of HMGCR partially reverses the effects of CAPRIN2 knockdown on 5-8F (E) and C666-1 (F) cell migration and invasion. Representative images of three independent experiments are shown. The data are expressed as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Migration, Over Expression, Multiple Displacement Amplification, GSH Assay, Stable Transfection, Expressing

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Regulation of lung colonization capacity via the CAPRIN2/HMGCR axis in NPC cells. (A, B) The inhibitory effect of erastin on the lung metastasis of NPC cells was enhanced by knockdown of the CAPRIN2/HMGCR axis. (C, D) CAPRIN2 promotes the lung colonization of NPC cells through HMGCR. For (A, C) , representative images of lungs and HE staining are shown. The location of lung metastatic nodules is indicated by the arrow. For (B, D) , the number of lung metastases (left panel) and the weight of the lungs (right panel) are given. * p < 0.05, ** p < 0.01.

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Staining

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: LINC00941 acts as an upstream molecule to regulate the biological functions of CAPRIN2. (A) LINC00941 downregulation promoted the ferroptosis of ECM-detached NPC cells, which was partially rescued by CAPRIN2 overexpression. The NPC cells were treated with erastin (5 μM) for 24 h (B) Knockdown of LINC00941 decreased the survival of ECM-detached NPC cells, which could be partially reversed by CAPRIN2 overexpression. For (A) and (B) , the assays were conducted in triplicate, and the data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01. (C, D) LINC00941 knockdown inhibited the migration and invasion of 5-8F (C) and C666-1 (D) cells, and overexpression of CAPRIN2 partially reversed this effect. Representative images are shown. The data are provided as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Over Expression, Migration

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: CAPRIN2 is overactivated in NPC tissues and is associated with a poor prognosis in patients. (A) The expression level of CAPRIN2 in 20 NPC tissues and 5 nasopharyngeal tissues. (B) The correlation between LINC00941/CAPRIN2, CAPRIN2/HMGCR or LINC00941/HMGCR in 20 NPC tissues. For (A) and (B) , the expression levels of CAPRIN2, HMGCR and LINC00941 were determined by qRT-PCR. The levels were normalized to those of β-actin and shown as the mean ± SEM. * p < 0.05. (C) Representative immunohistochemical images of normal nasopharyngeal tissues (left panel) and NPC tissues (middle panel). The boxes represent the magnified region. The representative image of negative stained control (right panel, top) shows the negative staining result of NPC tissues incubated with antibody-free serum. The representative image of positive stained control (right panel, bottom) shows the positive staining result of CAPRIN2 in NPC tissues incubated with the primary antibody of CAPRIN2. (D) Kaplan-Meier survival analysis of the association between CAPRIN2 expression and the PFS or OS of NPC patients ( log-rank test ).

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Staining, Negative Staining, Incubation

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Correlations between CAPRIN2 expression and clinicopathological characteristics.

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: CAPRIN2 upregulation by LINC00941 promotes nasopharyngeal carcinoma ferroptosis resistance and metastatic colonization through HMGCR

doi: 10.3389/fonc.2022.931749

Figure Lengend Snippet: Univariate and multivariate analysis for OS.

Article Snippet: To construct stable cell lines with knockdown of CAPRIN2 or HMGCR, lentiviruses carrying CAPRIN2 shRNA, HMGCR shRNA or scramble shRNA were purchased from Santa Cruz (CA, USA) and used to infect the indicated NPC cell lines for 48 h. The sequence of human LINC00941 shRNA was 5′- GAGACAGTTGATAGCCAAA -3′ , and the constructs were cloned into pHBLV-U6-MCS-PGK-PURO, named pHBLV-shLINC00941. pHBLV-shLINC00941 was transfected into 293T cells along with the corresponding packaging vector PMD2.G and pSPAX2.

Techniques: Expressing