r6 5  (ATCC)


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    ATCC r6 5
    R6 5, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    r6 5  (ATCC)


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    ATCC r6 5
    R6 5, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hybridoma cells  (ATCC)


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    ATCC hybridoma cells
    Hybridoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hb 9580 hybridoma  (ATCC)


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    ATCC hb 9580 hybridoma
    Hb 9580 Hybridoma, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hb 9580 hybridoma  (ATCC)


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    ATCC hb 9580 hybridoma
    Hb 9580 Hybridoma, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    r6 5 anti icam  (ATCC)


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    ATCC r6 5 anti icam
    Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to <t>ICAM-1</t> <t>(anti-ICAM</t> NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).
    R6 5 Anti Icam, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r6 5 anti icam/product/ATCC
    Average 92 stars, based on 1 article reviews
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    1) Product Images from "δ -Tocopherol Effect on Endocytosis and Its Combination with Enzyme Replacement Therapy for Lysosomal Disorders: A New Type of Drug Interaction? "

    Article Title: δ -Tocopherol Effect on Endocytosis and Its Combination with Enzyme Replacement Therapy for Lysosomal Disorders: A New Type of Drug Interaction?

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    doi: 10.1124/jpet.119.257345

    Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to ICAM-1 (anti-ICAM NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).
    Figure Legend Snippet: Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to ICAM-1 (anti-ICAM NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).

    Techniques Used: Microscopy, Incubation, Labeling

    δ-Tocopherol modulation of the TNFα effect on endocytosis in diseased endothelial cells. Imipramine-diseased endothelial cells (Dis) treated for 48 hours with 40 µM δ-tocopherol were left quiescent or activated overnight with TNFα to mimic inflammation. Cells were then incubated for 3 hours with (A) fluorescent anti-ICAM NCs or (B) fluorescent ligands of all individual endocytic pathways described in Fig. 3. Cells were washed and fixed, and cell surface ligands were fluorescently immunostained in a different color to distinguish internalized vs. surface-bound counterparts. (A) Binding was quantified as the total number of cell-associated fluorescent NCs, of which also the percentage of NCs internalized was measured. Data were normalized to untreated diseased cells (horizontal solid line). (B) Uptake of fluorescent ligands in TNFα-activated cells was normalized to nonactivated cells (horizontal solid line). (A and B) Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with nonactivated cells (P < 0.05 by Student’s t test).
    Figure Legend Snippet: δ-Tocopherol modulation of the TNFα effect on endocytosis in diseased endothelial cells. Imipramine-diseased endothelial cells (Dis) treated for 48 hours with 40 µM δ-tocopherol were left quiescent or activated overnight with TNFα to mimic inflammation. Cells were then incubated for 3 hours with (A) fluorescent anti-ICAM NCs or (B) fluorescent ligands of all individual endocytic pathways described in Fig. 3. Cells were washed and fixed, and cell surface ligands were fluorescently immunostained in a different color to distinguish internalized vs. surface-bound counterparts. (A) Binding was quantified as the total number of cell-associated fluorescent NCs, of which also the percentage of NCs internalized was measured. Data were normalized to untreated diseased cells (horizontal solid line). (B) Uptake of fluorescent ligands in TNFα-activated cells was normalized to nonactivated cells (horizontal solid line). (A and B) Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with nonactivated cells (P < 0.05 by Student’s t test).

    Techniques Used: Incubation, Binding Assay

    Effect of δ-tocopherol on uptake of recombinant ASM via the clathrin vs. CAM pathways. Imipramine-diseased endothelial cells (Dis), activated overnight with TNFα and treated for 48 hours with 40 µM δ-tocopherol, were incubated for 2 hours with 2.1 µg/ml naked 125I-ASM or 125I-ASM coupled to anti-ICAM NCs (anti-ICAM/ASM NCs). After washing cells, an acid glycine solution was used to elute noninternalized ASM from the cell surface. ASM delivered into cells was then measured in the cell lysates. (A) Internalized ASM in cell lysates. (B) ASM uptake after treatment with δ-tocopherol as a percentage of untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with naked ASM (P < 0.05 by Student’s t test).
    Figure Legend Snippet: Effect of δ-tocopherol on uptake of recombinant ASM via the clathrin vs. CAM pathways. Imipramine-diseased endothelial cells (Dis), activated overnight with TNFα and treated for 48 hours with 40 µM δ-tocopherol, were incubated for 2 hours with 2.1 µg/ml naked 125I-ASM or 125I-ASM coupled to anti-ICAM NCs (anti-ICAM/ASM NCs). After washing cells, an acid glycine solution was used to elute noninternalized ASM from the cell surface. ASM delivered into cells was then measured in the cell lysates. (A) Internalized ASM in cell lysates. (B) ASM uptake after treatment with δ-tocopherol as a percentage of untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with naked ASM (P < 0.05 by Student’s t test).

    Techniques Used: Recombinant, Incubation

    r6 5 anti icam  (ATCC)


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    ATCC r6 5 anti icam
    Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to <t>ICAM-1</t> <t>(anti-ICAM</t> NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).
    R6 5 Anti Icam, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r6 5 anti icam/product/ATCC
    Average 92 stars, based on 1 article reviews
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    r6 5 anti icam - by Bioz Stars, 2024-03
    92/100 stars

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    1) Product Images from "δ -Tocopherol Effect on Endocytosis and Its Combination with Enzyme Replacement Therapy for Lysosomal Disorders: A New Type of Drug Interaction? "

    Article Title: δ -Tocopherol Effect on Endocytosis and Its Combination with Enzyme Replacement Therapy for Lysosomal Disorders: A New Type of Drug Interaction?

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    doi: 10.1124/jpet.119.257345

    Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to ICAM-1 (anti-ICAM NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).
    Figure Legend Snippet: Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to ICAM-1 (anti-ICAM NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).

    Techniques Used: Microscopy, Incubation, Labeling

    δ-Tocopherol modulation of the TNFα effect on endocytosis in diseased endothelial cells. Imipramine-diseased endothelial cells (Dis) treated for 48 hours with 40 µM δ-tocopherol were left quiescent or activated overnight with TNFα to mimic inflammation. Cells were then incubated for 3 hours with (A) fluorescent anti-ICAM NCs or (B) fluorescent ligands of all individual endocytic pathways described in Fig. 3. Cells were washed and fixed, and cell surface ligands were fluorescently immunostained in a different color to distinguish internalized vs. surface-bound counterparts. (A) Binding was quantified as the total number of cell-associated fluorescent NCs, of which also the percentage of NCs internalized was measured. Data were normalized to untreated diseased cells (horizontal solid line). (B) Uptake of fluorescent ligands in TNFα-activated cells was normalized to nonactivated cells (horizontal solid line). (A and B) Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with nonactivated cells (P < 0.05 by Student’s t test).
    Figure Legend Snippet: δ-Tocopherol modulation of the TNFα effect on endocytosis in diseased endothelial cells. Imipramine-diseased endothelial cells (Dis) treated for 48 hours with 40 µM δ-tocopherol were left quiescent or activated overnight with TNFα to mimic inflammation. Cells were then incubated for 3 hours with (A) fluorescent anti-ICAM NCs or (B) fluorescent ligands of all individual endocytic pathways described in Fig. 3. Cells were washed and fixed, and cell surface ligands were fluorescently immunostained in a different color to distinguish internalized vs. surface-bound counterparts. (A) Binding was quantified as the total number of cell-associated fluorescent NCs, of which also the percentage of NCs internalized was measured. Data were normalized to untreated diseased cells (horizontal solid line). (B) Uptake of fluorescent ligands in TNFα-activated cells was normalized to nonactivated cells (horizontal solid line). (A and B) Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with nonactivated cells (P < 0.05 by Student’s t test).

    Techniques Used: Incubation, Binding Assay

    Effect of δ-tocopherol on uptake of recombinant ASM via the clathrin vs. CAM pathways. Imipramine-diseased endothelial cells (Dis), activated overnight with TNFα and treated for 48 hours with 40 µM δ-tocopherol, were incubated for 2 hours with 2.1 µg/ml naked 125I-ASM or 125I-ASM coupled to anti-ICAM NCs (anti-ICAM/ASM NCs). After washing cells, an acid glycine solution was used to elute noninternalized ASM from the cell surface. ASM delivered into cells was then measured in the cell lysates. (A) Internalized ASM in cell lysates. (B) ASM uptake after treatment with δ-tocopherol as a percentage of untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with naked ASM (P < 0.05 by Student’s t test).
    Figure Legend Snippet: Effect of δ-tocopherol on uptake of recombinant ASM via the clathrin vs. CAM pathways. Imipramine-diseased endothelial cells (Dis), activated overnight with TNFα and treated for 48 hours with 40 µM δ-tocopherol, were incubated for 2 hours with 2.1 µg/ml naked 125I-ASM or 125I-ASM coupled to anti-ICAM NCs (anti-ICAM/ASM NCs). After washing cells, an acid glycine solution was used to elute noninternalized ASM from the cell surface. ASM delivered into cells was then measured in the cell lysates. (A) Internalized ASM in cell lysates. (B) ASM uptake after treatment with δ-tocopherol as a percentage of untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with naked ASM (P < 0.05 by Student’s t test).

    Techniques Used: Recombinant, Incubation

    r6 5 anti icam  (ATCC)


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    ATCC r6 5 anti icam
    Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to <t>ICAM-1</t> <t>(anti-ICAM</t> NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).
    R6 5 Anti Icam, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r6 5 anti icam/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r6 5 anti icam - by Bioz Stars, 2024-03
    92/100 stars

    Images

    1) Product Images from "δ -Tocopherol Effect on Endocytosis and Its Combination with Enzyme Replacement Therapy for Lysosomal Disorders: A New Type of Drug Interaction? "

    Article Title: δ -Tocopherol Effect on Endocytosis and Its Combination with Enzyme Replacement Therapy for Lysosomal Disorders: A New Type of Drug Interaction?

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    doi: 10.1124/jpet.119.257345

    Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to ICAM-1 (anti-ICAM NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).
    Figure Legend Snippet: Effect of δ-tocopherol on receptor-mediated uptake in diseased endothelial cells. (A) Microscopy quantification of the uptake (2-hour) of fluorescent ligands of individual endocytic pathways in imipramine-diseased endothelial cells (Dis) vs. control (Ctrl) cells. (B) Uptake of fluorescent ligands (3-hour) in imiprimine-diseased cells treated for 48 hours with 40 µM δ-tocopherol under noninflammatory or (C) inflammatory-like conditions (overnight incubation with TNFα). Ligands were CTB (caveolae-mediated endocytosis), Tf (clathrin-mediated endocytosis), 200 nm polymer nanocarriers targeted to ICAM-1 (anti-ICAM NCs; CAM-mediated endocytosis), and 1 µm IgG-coated microparticles (phagocytosis; Phag.). (A–C) After ligand incubation, cells were washed and fixed, and cell surface–bound ligands were immunostained with antibodies fluorescently labeled in a different color to distinguish internalized vs. surface-bound localization (see Materials and Methods). Data are mean ± S.E.M. (n ≥ 4 independent wells), normalized to conditions shown in the horizontal solid lines. *Comparison with untreated diseased cells; †comparison with untreated diseased cells; #comparison with the CAM pathway; $comparison with the clathrin pathway (P < 0.05 by Student’s t test).

    Techniques Used: Microscopy, Incubation, Labeling

    δ-Tocopherol modulation of the TNFα effect on endocytosis in diseased endothelial cells. Imipramine-diseased endothelial cells (Dis) treated for 48 hours with 40 µM δ-tocopherol were left quiescent or activated overnight with TNFα to mimic inflammation. Cells were then incubated for 3 hours with (A) fluorescent anti-ICAM NCs or (B) fluorescent ligands of all individual endocytic pathways described in Fig. 3. Cells were washed and fixed, and cell surface ligands were fluorescently immunostained in a different color to distinguish internalized vs. surface-bound counterparts. (A) Binding was quantified as the total number of cell-associated fluorescent NCs, of which also the percentage of NCs internalized was measured. Data were normalized to untreated diseased cells (horizontal solid line). (B) Uptake of fluorescent ligands in TNFα-activated cells was normalized to nonactivated cells (horizontal solid line). (A and B) Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with nonactivated cells (P < 0.05 by Student’s t test).
    Figure Legend Snippet: δ-Tocopherol modulation of the TNFα effect on endocytosis in diseased endothelial cells. Imipramine-diseased endothelial cells (Dis) treated for 48 hours with 40 µM δ-tocopherol were left quiescent or activated overnight with TNFα to mimic inflammation. Cells were then incubated for 3 hours with (A) fluorescent anti-ICAM NCs or (B) fluorescent ligands of all individual endocytic pathways described in Fig. 3. Cells were washed and fixed, and cell surface ligands were fluorescently immunostained in a different color to distinguish internalized vs. surface-bound counterparts. (A) Binding was quantified as the total number of cell-associated fluorescent NCs, of which also the percentage of NCs internalized was measured. Data were normalized to untreated diseased cells (horizontal solid line). (B) Uptake of fluorescent ligands in TNFα-activated cells was normalized to nonactivated cells (horizontal solid line). (A and B) Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with nonactivated cells (P < 0.05 by Student’s t test).

    Techniques Used: Incubation, Binding Assay

    Effect of δ-tocopherol on uptake of recombinant ASM via the clathrin vs. CAM pathways. Imipramine-diseased endothelial cells (Dis), activated overnight with TNFα and treated for 48 hours with 40 µM δ-tocopherol, were incubated for 2 hours with 2.1 µg/ml naked 125I-ASM or 125I-ASM coupled to anti-ICAM NCs (anti-ICAM/ASM NCs). After washing cells, an acid glycine solution was used to elute noninternalized ASM from the cell surface. ASM delivered into cells was then measured in the cell lysates. (A) Internalized ASM in cell lysates. (B) ASM uptake after treatment with δ-tocopherol as a percentage of untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with naked ASM (P < 0.05 by Student’s t test).
    Figure Legend Snippet: Effect of δ-tocopherol on uptake of recombinant ASM via the clathrin vs. CAM pathways. Imipramine-diseased endothelial cells (Dis), activated overnight with TNFα and treated for 48 hours with 40 µM δ-tocopherol, were incubated for 2 hours with 2.1 µg/ml naked 125I-ASM or 125I-ASM coupled to anti-ICAM NCs (anti-ICAM/ASM NCs). After washing cells, an acid glycine solution was used to elute noninternalized ASM from the cell surface. ASM delivered into cells was then measured in the cell lysates. (A) Internalized ASM in cell lysates. (B) ASM uptake after treatment with δ-tocopherol as a percentage of untreated diseased cells (horizontal solid line). Data are mean ± S.E.M. (n ≥ 4 independent wells). *Comparison with untreated diseased cells; †comparison with naked ASM (P < 0.05 by Student’s t test).

    Techniques Used: Recombinant, Incubation

    hb 9580 hybridoma  (ATCC)


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    ATCC hb 9580 hybridoma
    Hb 9580 Hybridoma, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hb 9580 hybridoma/product/ATCC
    Average 92 stars, based on 1 article reviews
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    hb 9580 hybridoma - by Bioz Stars, 2024-03
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    mouse hb9580 r6 5 antibody against human icam 1  (ATCC)


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  • 92

    Structured Review

    ATCC mouse hb9580 r6 5 antibody against human icam 1
    Mouse Hb9580 R6 5 Antibody Against Human Icam 1, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse hb9580 r6 5 antibody against human icam 1/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse hb9580 r6 5 antibody against human icam 1 - by Bioz Stars, 2024-03
    92/100 stars

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    mouse hb9580 r6 5 antibody against human icam 1  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
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  • Bioz Stars
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  • 92

    Structured Review

    ATCC mouse hb9580 r6 5 antibody against human icam 1
    Mouse Hb9580 R6 5 Antibody Against Human Icam 1, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse hb9580 r6 5 antibody against human icam 1/product/ATCC
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse hb9580 r6 5 antibody against human icam 1 - by Bioz Stars, 2024-03
    92/100 stars

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