cd70  (ATCC)

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: In vitro characterization of [ 68 Ga]Ga-NOTA-anti-CD70 VHH. A Analysis of CD70 protein expression measured by flow cytometry using in the upper trace anti-CD70 VHH-Hilyte Fluor 488 (107B8) or control VHH-Hilyte Fluor 488 and in the lower trace PE mouse anti-human CD70 or PE mouse IgG3 isotype control. B Relative amount of cell associated activity of [ 68 Ga]Ga-NOTA-anti-CD70 VHH (5 nM, 0.04 µg) on CD70 high and CD70 low cells (1 × 10 6 ), or in the presence of excess unlabeled VHH (50 µg, block). (C) Specific binding of [ 68 Ga]Ga-NOTA-anti-CD70 VHH on Raji cells (5 × 10 5 ). (**** p < 0.0001)

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: In Vitro, Expressing, Flow Cytometry, Control, Activity Assay, Blocking Assay, Binding Assay

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: Biodistribution analysis of [ 68 Ga]Ga-NOTA-anti-CD70 VHH at 15, 30, 60 and 90 min post radiotracer injection. (%ID/g = % injected dose/ gram, injected radiotracer dose = 1–4.7 µg; ~ 1.5–2.5 MBq/mouse, n = 3/time point)

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Injection

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: PET/CT imaging using [ 68 Ga]Ga-NOTA-anti-CD70 VHH fragment— A Representative coronal µPET/CT images (30–60 min post injection timeframe summed activity, slice thickness 0.78 mm) of mice bearing CD70 high (786-O (left panel) and with block (middle panel)) and CD70 low tumors (NCl-H1975, right panel). B Time-activity curves for radiotracer distribution in the tumors are shown. (%ID/mL = % injected dose/ mL, injected radiotracer dose = 4–6.7 µg; ~ 6.4–7.4 MBq/mouse; n = 7, data are presented as mean ± SEM. **** p < 0.0001; #### p = 0.0029)

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Injection, Activity Assay, Blocking Assay

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: Ex vivo analysis of 786-O and NCl-H1975 tumors. Representative autoradiography (ARG) and microscopy images of adjacent histologic tumor slices stained for CD70 using a biotinylated anti-CD70 VHH

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Ex Vivo, Autoradiography, Microscopy, Staining

Journal: EJNMMI Radiopharmacy and Chemistry

Article Title: Site-specific 68 Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models

doi: 10.1186/s41181-023-00194-3

Figure Lengend Snippet: Binding affinity kinetics (equilibrium dissociation constant (K D ), association (k a ) and dissociation (k d ) rates) of anti-CD70 VHHs as determined by SPR

Article Snippet: CD70 expression on human Burkitt's lymphoma Raji (ATCC: CCL-86), renal cell carcinoma 786-O (ATCC: CRL-1932), and lung adenocarcinoma NCl-H1975 (ATCC: CRL-5908) cell lines was evaluated using flow cytometry analysis.

Techniques: Binding Assay

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: Schematic diagram showing conventional bivalent monospecific anti-CD4 and anti-CD70 IgGs along with anti-CD4/CD70 monovalent bispecific DuetMab.

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques:

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: Cell binding and ADCC activity of anti-CD4/CD70 DuetMab. ( A ) Anti-CD4/CD70 DuetMab exhibits preferential cell binding to CD4 + /CD70 + T cells via concurrent engagement to CD4 and CD70 on a single cell. ( B ) Anti-CD4/CD70 DuetMab preferentially kills CD4 + /CD70 + T cells as measured by ADCC. Each point represents the mean value of triplicate wells and the standard deviation is represented by error bars.

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques: Binding Assay, Activity Assay, Standard Deviation

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: CD4 and CD70 receptor density on human lymphocytes

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques:

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: Binding affinity of IgG and DuetMab to CD4 and CD70

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques: Binding Assay

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: Cell binding of various DuetMabs variants. Binding of anti-CD4/CD70 DuetMab variants to ( A ) CD4 + /CD70 + , ( B ) CD4 − /CD70 + and ( C ) CD4 + /CD70 − lymphocytes in a mixture of all 3 cell types. All variants with reduced affinity to CD4 exhibited improved binding selectivity over the parental DuetMab whereas their binding to target CD4 + /CD70 + T cells was not substantially impaired. Each point represents the mean values of triplicate wells and the standard deviation is represented by error bars.

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques: Binding Assay, Standard Deviation

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: ADCC activity of various DuetMabs variants. ( A ) Selective ADCC depletion of CD4 + /CD70 + T cells in a cell-mixture also containing non-target CD4 + /CD70 − T cells and CD4 − /CD70 + B cells. ( B ) ADCC activity of parental and anti-CD4 VκY94A+V H Y99A/CD70 DuetMabs against individual populations of CD4 + /CD70 + and CD4 + /CD70 − T-lymphocytes at varying E:T ratios. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques: Activity Assay, Standard Deviation

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: Cytotoxicity of various CD4 affinity-reduced DuetMab variants

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques:

Journal: mAbs

Article Title: Insights into the molecular basis of a bispecific antibody's target selectivity

doi: 10.1080/19420862.2015.1022695

Figure Lengend Snippet: Effect of antibody valence on cell binding and ADCC activity. ( A ) Cell binding and ( B ) ADCC activity of anti-CD4 VκY94A+V H Y99A/CD70 and 2 monospecific (anti-CD4 VκY94A+V H Y99A/NMGC and anti-CD70/NMGC) DuetMabs at equimolar concentration against CD4 + /CD70 + T cells alone. ( C ) Non-target ADCC activity of anti-CD4 variants formatted as either monovalent anti-CD4/CD70 DuetMab or bivalent anti-CD4 IgG against CD4 + /CD70 − T cells alone. Each point in these studies represents the mean values of triplicate wells and the standard deviation is represented by error bars.

Article Snippet: T1B-196 CD4 − /CD70 + B cells were obtained from the American Type Culture Collection and cultured in RPMI-1640 supplemented with 15% heat-inactivated (HI) fetal bovine serum (FBS).

Techniques: Binding Assay, Activity Assay, Concentration Assay, Standard Deviation