pco1  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ATCC pco1
    Bacterial strains and plasmids used in this study
    Pco1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pco1/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pco1 - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Regulation of the sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor"

    Article Title: Regulation of the sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor

    Journal:

    doi:

    Bacterial strains and plasmids used in this study
    Figure Legend Snippet: Bacterial strains and plasmids used in this study

    Techniques Used: Plasmid Preparation

    Schematic representations of the plasmids pSOLR (a), pCO1 (b), and pO1X (c). P is the solR promoter, and T1 and T2 are transcriptional terminators identified previously (42) downstream of solR. solR′ is the 0.9-kb internal fragment of solR (see construction of pO1X in Materials and Methods).
    Figure Legend Snippet: Schematic representations of the plasmids pSOLR (a), pCO1 (b), and pO1X (c). P is the solR promoter, and T1 and T2 are transcriptional terminators identified previously (42) downstream of solR. solR′ is the 0.9-kb internal fragment of solR (see construction of pO1X in Materials and Methods).

    Techniques Used:

    Product concentration and optical density (OD) profiles for controlled-pH (pH ≥ 4.5) batch fermentations with C. acetobutylicum strain ATCC 824(pCO1) (a) and mutant B (b). Zero time indicates the time at which the bioreactor was inoculated with a 1/10 (vol/vol) preculture. Symbols: ⊞, glucose; •, ethanol; ▴, acetone; ■, butanol; ○, acetate; □, butyrate; ▵, optical density at 600 nm; ⊙, pH.
    Figure Legend Snippet: Product concentration and optical density (OD) profiles for controlled-pH (pH ≥ 4.5) batch fermentations with C. acetobutylicum strain ATCC 824(pCO1) (a) and mutant B (b). Zero time indicates the time at which the bioreactor was inoculated with a 1/10 (vol/vol) preculture. Symbols: ⊞, glucose; •, ethanol; ▴, acetone; ■, butanol; ○, acetate; □, butyrate; ▵, optical density at 600 nm; ⊙, pH.

    Techniques Used: Concentration Assay, Mutagenesis

    Final product levels in C. acetobutylicum fermentor experiments at pH 4.5
    Figure Legend Snippet: Final product levels in C. acetobutylicum fermentor experiments at pH 4.5

    Techniques Used: Mutagenesis

    Primer extension analysis. Primer extension products made with primer BORFU-PE complementary to the N-terminal end of solR are shown. RNA for these experiments was obtained from C. acetobutylicum ATCC 824 cells (lanes 1 and 2) and from C. acetobutylicum ATCC 824(pCO1) cells (lanes 7 and 8). Cell samples were collected during early exponential (stage A, 5 h) and late exponential (stage B, 10 h) growth phases. The late exponential growth phase is also the solventogenic phase of ATCC 824 cells. Regions of the plasmid pCO1 were sequenced with the same primer, and the resulting DNA sequences are shown in lanes 3 to 6. The corresponding 5′-to-3′ DNA sequence of the complementary (coding) strand is indicated to the right of the gel (sequence continues onto a second line), wherein the boxed nucleotide is the transcriptional start site. The arrow indicates the position of the nearly invisible bands in lanes 1 and 2. The total RNA (20 μg) used for lanes 7 and 8 corresponding to strain ATCC 824(pCO1) was the same as that loaded in primer extension reactions performed previously (42) to map the transcriptional start site of aad. However, low transcriptional levels are characteristic of regulatory proteins, since they are present in low copy numbers and hence, on this suspicion, twice the standard amount of total RNA (40 μg) was used for lanes 1 and 2 (corresponding to wild-type strain ATCC 824) in order to obtain visible bands.
    Figure Legend Snippet: Primer extension analysis. Primer extension products made with primer BORFU-PE complementary to the N-terminal end of solR are shown. RNA for these experiments was obtained from C. acetobutylicum ATCC 824 cells (lanes 1 and 2) and from C. acetobutylicum ATCC 824(pCO1) cells (lanes 7 and 8). Cell samples were collected during early exponential (stage A, 5 h) and late exponential (stage B, 10 h) growth phases. The late exponential growth phase is also the solventogenic phase of ATCC 824 cells. Regions of the plasmid pCO1 were sequenced with the same primer, and the resulting DNA sequences are shown in lanes 3 to 6. The corresponding 5′-to-3′ DNA sequence of the complementary (coding) strand is indicated to the right of the gel (sequence continues onto a second line), wherein the boxed nucleotide is the transcriptional start site. The arrow indicates the position of the nearly invisible bands in lanes 1 and 2. The total RNA (20 μg) used for lanes 7 and 8 corresponding to strain ATCC 824(pCO1) was the same as that loaded in primer extension reactions performed previously (42) to map the transcriptional start site of aad. However, low transcriptional levels are characteristic of regulatory proteins, since they are present in low copy numbers and hence, on this suspicion, twice the standard amount of total RNA (40 μg) was used for lanes 1 and 2 (corresponding to wild-type strain ATCC 824) in order to obtain visible bands.

    Techniques Used: Plasmid Preparation, Sequencing

    Time course primer extension analysis of ATCC 824(pCO1) cells. RNA for the time course primer extension experiments was isolated from ATCC 824(pCO1) cells isolated during the early exponential growth (stage A, 5 h), late exponential growth (stage B, 10 h), early stationary (stage C, 25 h), and late stationary (stage D, 50 h) stages in a batch fermentation with a controlled pH (pH 4.5). The presence of mRNA corresponding to solR (lanes 1 to 4) aad (lanes 5 to 8) and adc (lanes 9 to 12) genes in each of the above four stages was verified by performing primer extension reactions with 20 μg of total RNA, using end-labeled 20-mer synthetic oligonucleotides BORFU-PE, BYDH-PE, and N-ADC that are complementary to the N-terminal ends of the respective genes.
    Figure Legend Snippet: Time course primer extension analysis of ATCC 824(pCO1) cells. RNA for the time course primer extension experiments was isolated from ATCC 824(pCO1) cells isolated during the early exponential growth (stage A, 5 h), late exponential growth (stage B, 10 h), early stationary (stage C, 25 h), and late stationary (stage D, 50 h) stages in a batch fermentation with a controlled pH (pH 4.5). The presence of mRNA corresponding to solR (lanes 1 to 4) aad (lanes 5 to 8) and adc (lanes 9 to 12) genes in each of the above four stages was verified by performing primer extension reactions with 20 μg of total RNA, using end-labeled 20-mer synthetic oligonucleotides BORFU-PE, BYDH-PE, and N-ADC that are complementary to the N-terminal ends of the respective genes.

    Techniques Used: Isolation, Labeling

    pco1  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    ATCC pco1
    Bacterial strains and plasmids used in this study
    Pco1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pco1/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pco1 - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Regulation of the sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor"

    Article Title: Regulation of the sol Locus Genes for Butanol and Acetone Formation in Clostridium acetobutylicum ATCC 824 by a Putative Transcriptional Repressor

    Journal:

    doi:

    Bacterial strains and plasmids used in this study
    Figure Legend Snippet: Bacterial strains and plasmids used in this study

    Techniques Used: Plasmid Preparation

    Schematic representations of the plasmids pSOLR (a), pCO1 (b), and pO1X (c). P is the solR promoter, and T1 and T2 are transcriptional terminators identified previously (42) downstream of solR. solR′ is the 0.9-kb internal fragment of solR (see construction of pO1X in Materials and Methods).
    Figure Legend Snippet: Schematic representations of the plasmids pSOLR (a), pCO1 (b), and pO1X (c). P is the solR promoter, and T1 and T2 are transcriptional terminators identified previously (42) downstream of solR. solR′ is the 0.9-kb internal fragment of solR (see construction of pO1X in Materials and Methods).

    Techniques Used:

    Product concentration and optical density (OD) profiles for controlled-pH (pH ≥ 4.5) batch fermentations with C. acetobutylicum strain ATCC 824(pCO1) (a) and mutant B (b). Zero time indicates the time at which the bioreactor was inoculated with a 1/10 (vol/vol) preculture. Symbols: ⊞, glucose; •, ethanol; ▴, acetone; ■, butanol; ○, acetate; □, butyrate; ▵, optical density at 600 nm; ⊙, pH.
    Figure Legend Snippet: Product concentration and optical density (OD) profiles for controlled-pH (pH ≥ 4.5) batch fermentations with C. acetobutylicum strain ATCC 824(pCO1) (a) and mutant B (b). Zero time indicates the time at which the bioreactor was inoculated with a 1/10 (vol/vol) preculture. Symbols: ⊞, glucose; •, ethanol; ▴, acetone; ■, butanol; ○, acetate; □, butyrate; ▵, optical density at 600 nm; ⊙, pH.

    Techniques Used: Concentration Assay, Mutagenesis

    Final product levels in C. acetobutylicum fermentor experiments at pH 4.5
    Figure Legend Snippet: Final product levels in C. acetobutylicum fermentor experiments at pH 4.5

    Techniques Used: Mutagenesis

    Primer extension analysis. Primer extension products made with primer BORFU-PE complementary to the N-terminal end of solR are shown. RNA for these experiments was obtained from C. acetobutylicum ATCC 824 cells (lanes 1 and 2) and from C. acetobutylicum ATCC 824(pCO1) cells (lanes 7 and 8). Cell samples were collected during early exponential (stage A, 5 h) and late exponential (stage B, 10 h) growth phases. The late exponential growth phase is also the solventogenic phase of ATCC 824 cells. Regions of the plasmid pCO1 were sequenced with the same primer, and the resulting DNA sequences are shown in lanes 3 to 6. The corresponding 5′-to-3′ DNA sequence of the complementary (coding) strand is indicated to the right of the gel (sequence continues onto a second line), wherein the boxed nucleotide is the transcriptional start site. The arrow indicates the position of the nearly invisible bands in lanes 1 and 2. The total RNA (20 μg) used for lanes 7 and 8 corresponding to strain ATCC 824(pCO1) was the same as that loaded in primer extension reactions performed previously (42) to map the transcriptional start site of aad. However, low transcriptional levels are characteristic of regulatory proteins, since they are present in low copy numbers and hence, on this suspicion, twice the standard amount of total RNA (40 μg) was used for lanes 1 and 2 (corresponding to wild-type strain ATCC 824) in order to obtain visible bands.
    Figure Legend Snippet: Primer extension analysis. Primer extension products made with primer BORFU-PE complementary to the N-terminal end of solR are shown. RNA for these experiments was obtained from C. acetobutylicum ATCC 824 cells (lanes 1 and 2) and from C. acetobutylicum ATCC 824(pCO1) cells (lanes 7 and 8). Cell samples were collected during early exponential (stage A, 5 h) and late exponential (stage B, 10 h) growth phases. The late exponential growth phase is also the solventogenic phase of ATCC 824 cells. Regions of the plasmid pCO1 were sequenced with the same primer, and the resulting DNA sequences are shown in lanes 3 to 6. The corresponding 5′-to-3′ DNA sequence of the complementary (coding) strand is indicated to the right of the gel (sequence continues onto a second line), wherein the boxed nucleotide is the transcriptional start site. The arrow indicates the position of the nearly invisible bands in lanes 1 and 2. The total RNA (20 μg) used for lanes 7 and 8 corresponding to strain ATCC 824(pCO1) was the same as that loaded in primer extension reactions performed previously (42) to map the transcriptional start site of aad. However, low transcriptional levels are characteristic of regulatory proteins, since they are present in low copy numbers and hence, on this suspicion, twice the standard amount of total RNA (40 μg) was used for lanes 1 and 2 (corresponding to wild-type strain ATCC 824) in order to obtain visible bands.

    Techniques Used: Plasmid Preparation, Sequencing

    Time course primer extension analysis of ATCC 824(pCO1) cells. RNA for the time course primer extension experiments was isolated from ATCC 824(pCO1) cells isolated during the early exponential growth (stage A, 5 h), late exponential growth (stage B, 10 h), early stationary (stage C, 25 h), and late stationary (stage D, 50 h) stages in a batch fermentation with a controlled pH (pH 4.5). The presence of mRNA corresponding to solR (lanes 1 to 4) aad (lanes 5 to 8) and adc (lanes 9 to 12) genes in each of the above four stages was verified by performing primer extension reactions with 20 μg of total RNA, using end-labeled 20-mer synthetic oligonucleotides BORFU-PE, BYDH-PE, and N-ADC that are complementary to the N-terminal ends of the respective genes.
    Figure Legend Snippet: Time course primer extension analysis of ATCC 824(pCO1) cells. RNA for the time course primer extension experiments was isolated from ATCC 824(pCO1) cells isolated during the early exponential growth (stage A, 5 h), late exponential growth (stage B, 10 h), early stationary (stage C, 25 h), and late stationary (stage D, 50 h) stages in a batch fermentation with a controlled pH (pH 4.5). The presence of mRNA corresponding to solR (lanes 1 to 4) aad (lanes 5 to 8) and adc (lanes 9 to 12) genes in each of the above four stages was verified by performing primer extension reactions with 20 μg of total RNA, using end-labeled 20-mer synthetic oligonucleotides BORFU-PE, BYDH-PE, and N-ADC that are complementary to the N-terminal ends of the respective genes.

    Techniques Used: Isolation, Labeling

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars

    • Image Search Results