e09 21 gid  (ATCC)


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    ATCC e09 21 gid
    E09 21 Gid, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e09 21 gid  (ATCC)


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    ATCC e09 21 gid
    E09 21 Gid, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1997b monilinia baccarum 951 2 norway vaccinium myrtillus z73773 z73746 holst jensen  (ATCC)


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    ATCC 1997b monilinia baccarum 951 2 norway vaccinium myrtillus z73773 z73746 holst jensen
    1997b Monilinia Baccarum 951 2 Norway Vaccinium Myrtillus Z73773 Z73746 Holst Jensen, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    951 amino acid protein  (ATCC)


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    ATCC 951 amino acid protein
    951 Amino Acid Protein, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dsm 2046  (ATCC)


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    ATCC dsm 2046
    Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ <xref ref-type= 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]." width="250" height="auto" />
    Dsm 2046, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enteropathogenic Potential of Bacillus thuringiensis Isolates from Soil, Animals, Food and Biopesticides"

    Article Title: Enteropathogenic Potential of Bacillus thuringiensis Isolates from Soil, Animals, Food and Biopesticides

    Journal: Foods

    doi: 10.3390/foods9101484

    Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ <xref ref-type= 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]." title="Overview of the 24 B. thuringiensis strains used in this study. Origin of ... " property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ].

    Techniques Used: Enzyme Immunoassay

    b thuringiensis  (ATCC)


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    ATCC b thuringiensis
    B Thuringiensis, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    decrease 3 log10 levels b thuringiensis 6 dsm 350 dsm 2046 dsm 5724 dsm 5815 dsm 6022 dsm 6087  (ATCC)


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    ATCC decrease 3 log10 levels b thuringiensis 6 dsm 350 dsm 2046 dsm 5724 dsm 5815 dsm 6022 dsm 6087
    Decrease 3 Log10 Levels B Thuringiensis 6 Dsm 350 Dsm 2046 Dsm 5724 Dsm 5815 Dsm 6022 Dsm 6087, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dsm 2046  (ATCC)


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    ATCC dsm 2046
    Labeling of Bacillus spp. cells with RBP reporters 1 .
    Dsm 2046, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Rapid Microscopic Detection of Bacillus anthracis by Fluorescent Receptor Binding Proteins of Bacteriophages"

    Article Title: Rapid Microscopic Detection of Bacillus anthracis by Fluorescent Receptor Binding Proteins of Bacteriophages

    Journal: Microorganisms

    doi: 10.3390/microorganisms8060934

    Labeling of Bacillus spp. cells with RBP reporters 1 .
    Figure Legend Snippet: Labeling of Bacillus spp. cells with RBP reporters 1 .

    Techniques Used: Labeling

    staining for βmhc  (ATCC)


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    ATCC staining for βmhc
    Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 <t>(Delta).</t> <t>Cardiomyocyte</t> proliferation was measured by histology as double-positive <t>βMHC</t> + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .
    Staining For βmhc, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Delta-1 Functionalized Hydrogel Promotes hESC-Cardiomyocyte Graft Proliferation and Maintains Heart Function Post-Injury"

    Article Title: Delta-1 Functionalized Hydrogel Promotes hESC-Cardiomyocyte Graft Proliferation and Maintains Heart Function Post-Injury

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1016/j.omtm.2020.04.011

    Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 (Delta). Cardiomyocyte proliferation was measured by histology as double-positive βMHC + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .
    Figure Legend Snippet: Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 (Delta). Cardiomyocyte proliferation was measured by histology as double-positive βMHC + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .

    Techniques Used: Activation Assay, Luciferase, Expressing

    Notch Signaling Enhances hESC-CM Engraftment and Proliferation at 1 Month Post-Implantation (A) Experimental timeline. Four days after ischemia/reperfusion injury, 5 × 10 6 hESC-CMs were transplanted in either IgG- or Delta-1-modified gel (control + hESC-CMs and Delta + hESC-CMs, respectively), and tissues were harvested after 4 weeks. (B and B′) Collagenous scar area is identified by picrosirius red staining with a fast green counterstain to label healthy myocardium. Outline identifies region of interest. Scale bars, 1 mm. (C and C′) In serial sections, human myocardial grafts are identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain. Scale bars, 1 mm. (D and D′) Regions of interest outlined in previous panels are shown at higher magnification with staining for βMHC. Scale bars, 250 μm. (E) Proliferation of transplanted hESC-MCs is identified by histology. Tissue sections are stained with antibodies to detect βMHC (green), BrdU (pink), and nuclear counterstain of DAPI (blue). Scale bar, 200 μm. Region of interest outlined in (E) is shown at higher magnification in (E′). (F) Graft area normalized to left ventricular area is significantly augmented in Delta + hESC-CMs. (G) Graft area normalized to infarct area is significantly augmented in Delta + hESC-CMs. (H) Engrafted cardiomyocyte proliferation identified by βMHC + /BrdU + cells is significantly enhanced in Delta + hESC-CMs. For (F)–(H), p-values were calculated using an unpaired two-tailed t-test. ∗p < 0.05, ∗∗p < 0.0001. Error bars represent SEM. See also .
    Figure Legend Snippet: Notch Signaling Enhances hESC-CM Engraftment and Proliferation at 1 Month Post-Implantation (A) Experimental timeline. Four days after ischemia/reperfusion injury, 5 × 10 6 hESC-CMs were transplanted in either IgG- or Delta-1-modified gel (control + hESC-CMs and Delta + hESC-CMs, respectively), and tissues were harvested after 4 weeks. (B and B′) Collagenous scar area is identified by picrosirius red staining with a fast green counterstain to label healthy myocardium. Outline identifies region of interest. Scale bars, 1 mm. (C and C′) In serial sections, human myocardial grafts are identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain. Scale bars, 1 mm. (D and D′) Regions of interest outlined in previous panels are shown at higher magnification with staining for βMHC. Scale bars, 250 μm. (E) Proliferation of transplanted hESC-MCs is identified by histology. Tissue sections are stained with antibodies to detect βMHC (green), BrdU (pink), and nuclear counterstain of DAPI (blue). Scale bar, 200 μm. Region of interest outlined in (E) is shown at higher magnification in (E′). (F) Graft area normalized to left ventricular area is significantly augmented in Delta + hESC-CMs. (G) Graft area normalized to infarct area is significantly augmented in Delta + hESC-CMs. (H) Engrafted cardiomyocyte proliferation identified by βMHC + /BrdU + cells is significantly enhanced in Delta + hESC-CMs. For (F)–(H), p-values were calculated using an unpaired two-tailed t-test. ∗p < 0.05, ∗∗p < 0.0001. Error bars represent SEM. See also .

    Techniques Used: Modification, Staining, Two Tailed Test

    Notch Signaling Enhances Vascularization with Similar Infarct Size at 1 Month Post-Implantation (A) Average infarct area is assessed by histology and normalized to left ventricular (LV) area at 4 weeks. (B) Anterior wall thickness by histology is shown for an area approximately 4 mm from the apex of the heart. Values are in mm. (C and D) Picrosirius red and fast green counterstain were used to identify fibrotic regions that are quantified in (A). Representative images are shown for both control + hESC-CMs (C) and Delta + hESC-CMs (D), where the black arrow identifies a region of human myocardial graft within the infarct. Scale bars, 2.5 mm. (E and F) Inflammatory response at 4 weeks by CD68 staining normalized to infarct area. Outlined regions of interest in (C) and (D) are shown at higher magnification in (E) and (F). Serial sections are stained with CD68 antibody to label monocytes and macrophages and visualized with diaminobenzidine (DAB) (brown). Scale bars, 200 μm. (G and H) Host-derived vessels are identified by staining with CD31 antibody (red) (G and H) with a double stain for βMHC (green) (G) to identify hESC-CM grafts within the infarct regions. Scale bar, 200 μm. (I) Level of inflammatory response is expressed as CD68 + area normalized to scar area by picrosirius red at 2 and 4 weeks. Values for 2 week time point were obtained through pilot study experiments shown in <xref ref-type=Figure S4 . (J) Level of neovascularization is quantified as CD31 + lumens within βMHC + graft regions. p-values were calculated using an unpaired two-tailed t-test. For (A), (B), (F), and (G), ∗p < 0.05. Error bars indicate SEM. " title="... (G and H) with a double stain for βMHC (green) (G) to identify hESC-CM grafts within the ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Notch Signaling Enhances Vascularization with Similar Infarct Size at 1 Month Post-Implantation (A) Average infarct area is assessed by histology and normalized to left ventricular (LV) area at 4 weeks. (B) Anterior wall thickness by histology is shown for an area approximately 4 mm from the apex of the heart. Values are in mm. (C and D) Picrosirius red and fast green counterstain were used to identify fibrotic regions that are quantified in (A). Representative images are shown for both control + hESC-CMs (C) and Delta + hESC-CMs (D), where the black arrow identifies a region of human myocardial graft within the infarct. Scale bars, 2.5 mm. (E and F) Inflammatory response at 4 weeks by CD68 staining normalized to infarct area. Outlined regions of interest in (C) and (D) are shown at higher magnification in (E) and (F). Serial sections are stained with CD68 antibody to label monocytes and macrophages and visualized with diaminobenzidine (DAB) (brown). Scale bars, 200 μm. (G and H) Host-derived vessels are identified by staining with CD31 antibody (red) (G and H) with a double stain for βMHC (green) (G) to identify hESC-CM grafts within the infarct regions. Scale bar, 200 μm. (I) Level of inflammatory response is expressed as CD68 + area normalized to scar area by picrosirius red at 2 and 4 weeks. Values for 2 week time point were obtained through pilot study experiments shown in Figure S4 . (J) Level of neovascularization is quantified as CD31 + lumens within βMHC + graft regions. p-values were calculated using an unpaired two-tailed t-test. For (A), (B), (F), and (G), ∗p < 0.05. Error bars indicate SEM.

    Techniques Used: Staining, Derivative Assay, Two Tailed Test

    Human Myocardial Graft Area is Significantly Increased 3 Months after Implantation with Notch Signaling (A) Experimental timeline. Four days after ischemia/reperfusion injury, 2.5 × 10 6 hESC-CMs were transplanted within the IgG control gel or the Delta-1 gel (control + hESC-CMs and Delta + hESC-CMs, respectively), with two additional gel-only control groups (control-no cells and Delta-no cells). (B and B′) Representative images of hESC-CM grafts identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain for control + hESC-CMs (B) and Delta + hESC-CMs (B′). Scale bars, 200 μm. (C) βMHC + graft area is normalized to the LV area. There is a substantial increase in graft area with the Delta + hESC-CMs. (D) Proliferating hESC-CMs are identified by double-labeled βMHC + /BrdU + cells, and quantification is shown here. There is a significant increase in Delta + hESC-CM proliferation. (E) Infarct area, quantified by the picrosirius red area, is normalized to the LV area. There is a modest, non-significant reduction in infarct size with Delta + hESC-CM treatment. (F) Anterior wall thickness is shown in mm. There is a modest, non-significant increase in anterior wall thickness in the Delta + hESC-CMs. For (C)–(F), ∗p < 0.05. Error bars indicate SEM. p-values were calculated for (C) and (D) using an unpaired t-test and for (E) and (F) using a one-way ANOVA followed by Sidak’s multiple comparison test comparing control-no cells versus Delta-no cells, control-no cells versus control + hESC-CMs, Delta-no cells versus Delta + hESC-CMs, and control + hESC-CMs versus Delta + hESC-CMs.
    Figure Legend Snippet: Human Myocardial Graft Area is Significantly Increased 3 Months after Implantation with Notch Signaling (A) Experimental timeline. Four days after ischemia/reperfusion injury, 2.5 × 10 6 hESC-CMs were transplanted within the IgG control gel or the Delta-1 gel (control + hESC-CMs and Delta + hESC-CMs, respectively), with two additional gel-only control groups (control-no cells and Delta-no cells). (B and B′) Representative images of hESC-CM grafts identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain for control + hESC-CMs (B) and Delta + hESC-CMs (B′). Scale bars, 200 μm. (C) βMHC + graft area is normalized to the LV area. There is a substantial increase in graft area with the Delta + hESC-CMs. (D) Proliferating hESC-CMs are identified by double-labeled βMHC + /BrdU + cells, and quantification is shown here. There is a significant increase in Delta + hESC-CM proliferation. (E) Infarct area, quantified by the picrosirius red area, is normalized to the LV area. There is a modest, non-significant reduction in infarct size with Delta + hESC-CM treatment. (F) Anterior wall thickness is shown in mm. There is a modest, non-significant increase in anterior wall thickness in the Delta + hESC-CMs. For (C)–(F), ∗p < 0.05. Error bars indicate SEM. p-values were calculated for (C) and (D) using an unpaired t-test and for (E) and (F) using a one-way ANOVA followed by Sidak’s multiple comparison test comparing control-no cells versus Delta-no cells, control-no cells versus control + hESC-CMs, Delta-no cells versus Delta + hESC-CMs, and control + hESC-CMs versus Delta + hESC-CMs.

    Techniques Used: Staining, Labeling

    beta myosin heavy chain  (ATCC)


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    ATCC beta myosin heavy chain
    Beta Myosin Heavy Chain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a fumigatus iam 2046  (ATCC)


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    ATCC a fumigatus iam 2046
    Antibacterial activity of guanidyl-polyol macrolide antibiotics.
    A Fumigatus Iam 2046, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Guanidine-Containing Polyhydroxyl Macrolides: Chemistry, Biology, and Structure-Activity Relationship"

    Article Title: Guanidine-Containing Polyhydroxyl Macrolides: Chemistry, Biology, and Structure-Activity Relationship

    Journal: Molecules

    doi: 10.3390/molecules24213913

    Antibacterial activity of guanidyl-polyol macrolide antibiotics.
    Figure Legend Snippet: Antibacterial activity of guanidyl-polyol macrolide antibiotics.

    Techniques Used: Activity Assay, Inhibition

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    ATCC 951 amino acid protein
    951 Amino Acid Protein, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC dsm 2046
    Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ <xref ref-type= 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]." width="250" height="auto" />
    Dsm 2046, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC b thuringiensis
    Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ <xref ref-type= 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]." width="250" height="auto" />
    B Thuringiensis, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC decrease 3 log10 levels b thuringiensis 6 dsm 350 dsm 2046 dsm 5724 dsm 5815 dsm 6022 dsm 6087
    Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ <xref ref-type= 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]." width="250" height="auto" />
    Decrease 3 Log10 Levels B Thuringiensis 6 Dsm 350 Dsm 2046 Dsm 5724 Dsm 5815 Dsm 6022 Dsm 6087, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC staining for βmhc
    Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 <t>(Delta).</t> <t>Cardiomyocyte</t> proliferation was measured by histology as double-positive <t>βMHC</t> + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .
    Staining For βmhc, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC beta myosin heavy chain
    Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 <t>(Delta).</t> <t>Cardiomyocyte</t> proliferation was measured by histology as double-positive <t>βMHC</t> + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .
    Beta Myosin Heavy Chain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC a fumigatus iam 2046
    Antibacterial activity of guanidyl-polyol macrolide antibiotics.
    A Fumigatus Iam 2046, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ <xref ref-type= 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]." width="100%" height="100%">

    Journal: Foods

    Article Title: Enteropathogenic Potential of Bacillus thuringiensis Isolates from Soil, Animals, Food and Biopesticides

    doi: 10.3390/foods9101484

    Figure Lengend Snippet: Overview of the 24 B. thuringiensis strains used in this study. Origin of the isolates: A: animal, F: food, P: pesticide, S: soil, U: undefined. +: positive PCR result for toxin gene. −: negative PCR result for toxin gene. *: NheB production determined in sandwich enzyme immunoassays (EIAs) after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ]. **: Cytotoxicity towards CaCo-2 cells after growth under simulated intestinal conditions according to Jessberger et al. [ 15 ].

    Article Snippet: 2873 , B. thuringiensis Berliner 1915, ATCC ® 10792™, DSM 2046 , Mediterranean flour moth , A , IV , 1 , + , + , − , − , + , High , High.

    Techniques: Enzyme Immunoassay

    Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 (Delta). Cardiomyocyte proliferation was measured by histology as double-positive βMHC + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Delta-1 Functionalized Hydrogel Promotes hESC-Cardiomyocyte Graft Proliferation and Maintains Heart Function Post-Injury

    doi: 10.1016/j.omtm.2020.04.011

    Figure Lengend Snippet: Prolonged Notch Activation in 3D Gels Is Dose-Dependent and Promotes hESC-CM Proliferation (A) Time course analysis of Notch-mediated luciferase expression in U2OS CSLluc/ren Notch reporter cell line indicates a prolonged Notch signal response in 3D culture conditions compared to the 2D Delta-1 platform. Luciferase signal is expressed normalized to IgG controls (2D or 3D) and plotted against days in culture. IgG controls were normalized to 1 (dashed line). (B) Dose-dependent Notch activation. Culturing the U2OS CSLluc/ren Notch reporter cell line in the 3D Delta-1 gel results in Delta-1 dose-dependent activation of Notch signaling, as indicated by fold luciferase expression compared to IgG control gels. (C) hESC-CMs in 3D-engineered cardiac tissues proliferate in response to Delta-1 (Delta). Cardiomyocyte proliferation was measured by histology as double-positive βMHC + /BrdU + cells, which results in a significant increase in response to Delta with day 15 cells and day 30 cells over IgG controls (control). Note that although proliferation slows over time, significant augmentation by Delta is still possible at day 30. For (B), p-values were calculated using a one-way ANOVA with all samples compared to 0 μg/mL followed by Dunnett’s multiple comparison test. For (C), p-values were calculated using a multiple unpaired t-test without assuming a consistent SD. ∗p < 0.05, ∗∗p < 0.005. Error bars denote SEM. See also and .

    Article Snippet: Human cardiomyocyte grafts were identified by staining for βMHC (hybridoma supernatant, ATCC #CRL-2046) and visualized using either Alexa Fluor 488 goat anti-mouse (1:100, Molecular Probes) or an avidin-biotin goat anti-mouse antibody (1:100, Vector Laboratories) developed with diaminobenzidene (Vector Laboratories).

    Techniques: Activation Assay, Luciferase, Expressing

    Notch Signaling Enhances hESC-CM Engraftment and Proliferation at 1 Month Post-Implantation (A) Experimental timeline. Four days after ischemia/reperfusion injury, 5 × 10 6 hESC-CMs were transplanted in either IgG- or Delta-1-modified gel (control + hESC-CMs and Delta + hESC-CMs, respectively), and tissues were harvested after 4 weeks. (B and B′) Collagenous scar area is identified by picrosirius red staining with a fast green counterstain to label healthy myocardium. Outline identifies region of interest. Scale bars, 1 mm. (C and C′) In serial sections, human myocardial grafts are identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain. Scale bars, 1 mm. (D and D′) Regions of interest outlined in previous panels are shown at higher magnification with staining for βMHC. Scale bars, 250 μm. (E) Proliferation of transplanted hESC-MCs is identified by histology. Tissue sections are stained with antibodies to detect βMHC (green), BrdU (pink), and nuclear counterstain of DAPI (blue). Scale bar, 200 μm. Region of interest outlined in (E) is shown at higher magnification in (E′). (F) Graft area normalized to left ventricular area is significantly augmented in Delta + hESC-CMs. (G) Graft area normalized to infarct area is significantly augmented in Delta + hESC-CMs. (H) Engrafted cardiomyocyte proliferation identified by βMHC + /BrdU + cells is significantly enhanced in Delta + hESC-CMs. For (F)–(H), p-values were calculated using an unpaired two-tailed t-test. ∗p < 0.05, ∗∗p < 0.0001. Error bars represent SEM. See also .

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Delta-1 Functionalized Hydrogel Promotes hESC-Cardiomyocyte Graft Proliferation and Maintains Heart Function Post-Injury

    doi: 10.1016/j.omtm.2020.04.011

    Figure Lengend Snippet: Notch Signaling Enhances hESC-CM Engraftment and Proliferation at 1 Month Post-Implantation (A) Experimental timeline. Four days after ischemia/reperfusion injury, 5 × 10 6 hESC-CMs were transplanted in either IgG- or Delta-1-modified gel (control + hESC-CMs and Delta + hESC-CMs, respectively), and tissues were harvested after 4 weeks. (B and B′) Collagenous scar area is identified by picrosirius red staining with a fast green counterstain to label healthy myocardium. Outline identifies region of interest. Scale bars, 1 mm. (C and C′) In serial sections, human myocardial grafts are identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain. Scale bars, 1 mm. (D and D′) Regions of interest outlined in previous panels are shown at higher magnification with staining for βMHC. Scale bars, 250 μm. (E) Proliferation of transplanted hESC-MCs is identified by histology. Tissue sections are stained with antibodies to detect βMHC (green), BrdU (pink), and nuclear counterstain of DAPI (blue). Scale bar, 200 μm. Region of interest outlined in (E) is shown at higher magnification in (E′). (F) Graft area normalized to left ventricular area is significantly augmented in Delta + hESC-CMs. (G) Graft area normalized to infarct area is significantly augmented in Delta + hESC-CMs. (H) Engrafted cardiomyocyte proliferation identified by βMHC + /BrdU + cells is significantly enhanced in Delta + hESC-CMs. For (F)–(H), p-values were calculated using an unpaired two-tailed t-test. ∗p < 0.05, ∗∗p < 0.0001. Error bars represent SEM. See also .

    Article Snippet: Human cardiomyocyte grafts were identified by staining for βMHC (hybridoma supernatant, ATCC #CRL-2046) and visualized using either Alexa Fluor 488 goat anti-mouse (1:100, Molecular Probes) or an avidin-biotin goat anti-mouse antibody (1:100, Vector Laboratories) developed with diaminobenzidene (Vector Laboratories).

    Techniques: Modification, Staining, Two Tailed Test

    Notch Signaling Enhances Vascularization with Similar Infarct Size at 1 Month Post-Implantation (A) Average infarct area is assessed by histology and normalized to left ventricular (LV) area at 4 weeks. (B) Anterior wall thickness by histology is shown for an area approximately 4 mm from the apex of the heart. Values are in mm. (C and D) Picrosirius red and fast green counterstain were used to identify fibrotic regions that are quantified in (A). Representative images are shown for both control + hESC-CMs (C) and Delta + hESC-CMs (D), where the black arrow identifies a region of human myocardial graft within the infarct. Scale bars, 2.5 mm. (E and F) Inflammatory response at 4 weeks by CD68 staining normalized to infarct area. Outlined regions of interest in (C) and (D) are shown at higher magnification in (E) and (F). Serial sections are stained with CD68 antibody to label monocytes and macrophages and visualized with diaminobenzidine (DAB) (brown). Scale bars, 200 μm. (G and H) Host-derived vessels are identified by staining with CD31 antibody (red) (G and H) with a double stain for βMHC (green) (G) to identify hESC-CM grafts within the infarct regions. Scale bar, 200 μm. (I) Level of inflammatory response is expressed as CD68 + area normalized to scar area by picrosirius red at 2 and 4 weeks. Values for 2 week time point were obtained through pilot study experiments shown in <xref ref-type=Figure S4 . (J) Level of neovascularization is quantified as CD31 + lumens within βMHC + graft regions. p-values were calculated using an unpaired two-tailed t-test. For (A), (B), (F), and (G), ∗p < 0.05. Error bars indicate SEM. " width="100%" height="100%">

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Delta-1 Functionalized Hydrogel Promotes hESC-Cardiomyocyte Graft Proliferation and Maintains Heart Function Post-Injury

    doi: 10.1016/j.omtm.2020.04.011

    Figure Lengend Snippet: Notch Signaling Enhances Vascularization with Similar Infarct Size at 1 Month Post-Implantation (A) Average infarct area is assessed by histology and normalized to left ventricular (LV) area at 4 weeks. (B) Anterior wall thickness by histology is shown for an area approximately 4 mm from the apex of the heart. Values are in mm. (C and D) Picrosirius red and fast green counterstain were used to identify fibrotic regions that are quantified in (A). Representative images are shown for both control + hESC-CMs (C) and Delta + hESC-CMs (D), where the black arrow identifies a region of human myocardial graft within the infarct. Scale bars, 2.5 mm. (E and F) Inflammatory response at 4 weeks by CD68 staining normalized to infarct area. Outlined regions of interest in (C) and (D) are shown at higher magnification in (E) and (F). Serial sections are stained with CD68 antibody to label monocytes and macrophages and visualized with diaminobenzidine (DAB) (brown). Scale bars, 200 μm. (G and H) Host-derived vessels are identified by staining with CD31 antibody (red) (G and H) with a double stain for βMHC (green) (G) to identify hESC-CM grafts within the infarct regions. Scale bar, 200 μm. (I) Level of inflammatory response is expressed as CD68 + area normalized to scar area by picrosirius red at 2 and 4 weeks. Values for 2 week time point were obtained through pilot study experiments shown in Figure S4 . (J) Level of neovascularization is quantified as CD31 + lumens within βMHC + graft regions. p-values were calculated using an unpaired two-tailed t-test. For (A), (B), (F), and (G), ∗p < 0.05. Error bars indicate SEM.

    Article Snippet: Human cardiomyocyte grafts were identified by staining for βMHC (hybridoma supernatant, ATCC #CRL-2046) and visualized using either Alexa Fluor 488 goat anti-mouse (1:100, Molecular Probes) or an avidin-biotin goat anti-mouse antibody (1:100, Vector Laboratories) developed with diaminobenzidene (Vector Laboratories).

    Techniques: Staining, Derivative Assay, Two Tailed Test

    Human Myocardial Graft Area is Significantly Increased 3 Months after Implantation with Notch Signaling (A) Experimental timeline. Four days after ischemia/reperfusion injury, 2.5 × 10 6 hESC-CMs were transplanted within the IgG control gel or the Delta-1 gel (control + hESC-CMs and Delta + hESC-CMs, respectively), with two additional gel-only control groups (control-no cells and Delta-no cells). (B and B′) Representative images of hESC-CM grafts identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain for control + hESC-CMs (B) and Delta + hESC-CMs (B′). Scale bars, 200 μm. (C) βMHC + graft area is normalized to the LV area. There is a substantial increase in graft area with the Delta + hESC-CMs. (D) Proliferating hESC-CMs are identified by double-labeled βMHC + /BrdU + cells, and quantification is shown here. There is a significant increase in Delta + hESC-CM proliferation. (E) Infarct area, quantified by the picrosirius red area, is normalized to the LV area. There is a modest, non-significant reduction in infarct size with Delta + hESC-CM treatment. (F) Anterior wall thickness is shown in mm. There is a modest, non-significant increase in anterior wall thickness in the Delta + hESC-CMs. For (C)–(F), ∗p < 0.05. Error bars indicate SEM. p-values were calculated for (C) and (D) using an unpaired t-test and for (E) and (F) using a one-way ANOVA followed by Sidak’s multiple comparison test comparing control-no cells versus Delta-no cells, control-no cells versus control + hESC-CMs, Delta-no cells versus Delta + hESC-CMs, and control + hESC-CMs versus Delta + hESC-CMs.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Delta-1 Functionalized Hydrogel Promotes hESC-Cardiomyocyte Graft Proliferation and Maintains Heart Function Post-Injury

    doi: 10.1016/j.omtm.2020.04.011

    Figure Lengend Snippet: Human Myocardial Graft Area is Significantly Increased 3 Months after Implantation with Notch Signaling (A) Experimental timeline. Four days after ischemia/reperfusion injury, 2.5 × 10 6 hESC-CMs were transplanted within the IgG control gel or the Delta-1 gel (control + hESC-CMs and Delta + hESC-CMs, respectively), with two additional gel-only control groups (control-no cells and Delta-no cells). (B and B′) Representative images of hESC-CM grafts identified by staining for β myosin heavy chain (βMHC, brown) with hematoxylin counterstain for control + hESC-CMs (B) and Delta + hESC-CMs (B′). Scale bars, 200 μm. (C) βMHC + graft area is normalized to the LV area. There is a substantial increase in graft area with the Delta + hESC-CMs. (D) Proliferating hESC-CMs are identified by double-labeled βMHC + /BrdU + cells, and quantification is shown here. There is a significant increase in Delta + hESC-CM proliferation. (E) Infarct area, quantified by the picrosirius red area, is normalized to the LV area. There is a modest, non-significant reduction in infarct size with Delta + hESC-CM treatment. (F) Anterior wall thickness is shown in mm. There is a modest, non-significant increase in anterior wall thickness in the Delta + hESC-CMs. For (C)–(F), ∗p < 0.05. Error bars indicate SEM. p-values were calculated for (C) and (D) using an unpaired t-test and for (E) and (F) using a one-way ANOVA followed by Sidak’s multiple comparison test comparing control-no cells versus Delta-no cells, control-no cells versus control + hESC-CMs, Delta-no cells versus Delta + hESC-CMs, and control + hESC-CMs versus Delta + hESC-CMs.

    Article Snippet: Human cardiomyocyte grafts were identified by staining for βMHC (hybridoma supernatant, ATCC #CRL-2046) and visualized using either Alexa Fluor 488 goat anti-mouse (1:100, Molecular Probes) or an avidin-biotin goat anti-mouse antibody (1:100, Vector Laboratories) developed with diaminobenzidene (Vector Laboratories).

    Techniques: Staining, Labeling

    Antibacterial activity of guanidyl-polyol macrolide antibiotics.

    Journal: Molecules

    Article Title: Guanidine-Containing Polyhydroxyl Macrolides: Chemistry, Biology, and Structure-Activity Relationship

    doi: 10.3390/molecules24213913

    Figure Lengend Snippet: Antibacterial activity of guanidyl-polyol macrolide antibiotics.

    Article Snippet: 28 , Five isolates of C. albicans (1.56~12.5), Trichophyton gypseum , (1.56–15.6), Fusarium graminarum (3.12~7.8), B. subtillis (6.25). 15.6), B. subtilis (31.25)., T. mentagrophytes (3.91–10), T. rubrum (1.95), T. asteroids UC-4775 (1), M. canis (0.48), M. canis UC-1395 (10), Cryptococcus immnzitis UC-1119 (1), C. neoformans UC-1139 (1), A. niger (3.91), A. fumigatus IAM 2046 (12.5), B. subtilis ATCC 6633 (16), B. subtilis UC-564 (4), B. dermatitidlis UC-1911 (1), F. culmorum JP 15 (25), Geotrichutim sp. UC-1207 (1), Hormodendrulmn compactum UC-1222 (1), Hormodendrulmn capsulatum UC-1220 (0.1), Nocardia asteroidles UC-2052 (10), Phialophora verrlucosai UC-1807 (1), 9 isolates of S. aureus (6.25–16), Streptococcus hemolyticus UC- 15 (31), S. pyogenes 308 (25), S. pyogenes 77 A (25), S. faecium D (50), Staphylococcus faecalis UC-3235 (31), 3 isolates of S. epidermidis (32), S. schenlckii UC-1364 (10), 7 isolates of Enterococcus faecalis (32~64), A. fumigatus IAM 2046 (MCC, >200) , [ , , , , , , ] .

    Techniques: Activity Assay, Inhibition